LOCUS       NM_008585               2923 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus meprin 1 alpha (Mep1a), mRNA.
ACCESSION   NM_008585
VERSION     NM_008585.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Bulck,C., Nystrom,E.E.L., Koudelka,T., Mannbar-Frahm,M.,
            Andresen,G., Radhouani,M., Tran,F., Scharfenberg,F., Schrell,F.,
            Armbrust,F., Dahlke,E., Zhao,B., Vervaeke,A., Theilig,F.,
            Rosenstiel,P., Starkl,P., Rosshart,S.P., Fickenscher,H., Tholey,A.,
            Hansson,G.C. and Becker-Pauly,C.
  TITLE     Proteolytic processing of galectin-3 by meprin metalloproteases is
            crucial for host-microbiome homeostasis
  JOURNAL   Sci Adv 9 (13), eadf4055 (2023)
   PUBMED   37000885
REFERENCE   3  (bases 1 to 2923)
  AUTHORS   Grainger,A.T., Pilar,N., Li,J., Chen,M.H., Abramson,A.M.,
            Becker-Pauly,C. and Shi,W.
  TITLE     Identification of Mep1a as a susceptibility gene for
            atherosclerosis in mice
  JOURNAL   Genetics 219 (4) (2021)
   PUBMED   34849841
  REMARK    GeneRIF: Identification of Mep1a as a susceptibility gene for
            atherosclerosis in mice.
REFERENCE   4  (bases 1 to 2923)
  AUTHORS   Ge,W., Hou,C., Zhang,W., Guo,X., Gao,P., Song,X., Gao,R., Liu,Y.,
            Guo,W., Li,B., Zhao,H. and Wang,J.
  TITLE     Mep1a contributes to Ang II-induced cardiac remodeling by promoting
            cardiac hypertrophy, fibrosis and inflammation
  JOURNAL   J Mol Cell Cardiol 152, 52-68 (2021)
   PUBMED   33301800
  REMARK    GeneRIF: Mep1a contributes to Ang II-induced cardiac remodeling by
            promoting cardiac hypertrophy, fibrosis and inflammation.
REFERENCE   5  (bases 1 to 2923)
  AUTHORS   Ahmed,F., Mwiza,J.M., Fernander,M., Yahaya,I., Abousaad,S. and
            Ongeri,E.M.
  TITLE     Meprin-beta activity modulates the beta-catalytic subunit of
            protein kinase A in ischemia-reperfusion-induced acute kidney
            injury
  JOURNAL   Am J Physiol Renal Physiol 318 (5), F1147-F1159 (2020)
   PUBMED   32174142
REFERENCE   6  (bases 1 to 2923)
  AUTHORS   Jiang,W., Gorbea,C.M., Flannery,A.V., Beynon,R.J., Grant,G.A. and
            Bond,J.S.
  TITLE     The alpha subunit of meprin A. Molecular cloning and sequencing,
            differential expression in inbred mouse strains, and evidence for
            divergent evolution of the alpha and beta subunits
  JOURNAL   J Biol Chem 267 (13), 9185-9193 (1992)
   PUBMED   1374387
  REMARK    Erratum:[J Biol Chem 1992 Jul 5;267(19):13779]
REFERENCE   7  (bases 1 to 2923)
  AUTHORS   Dumermuth,E., Sterchi,E.E., Jiang,W.P., Wolz,R.L., Bond,J.S.,
            Flannery,A.V. and Beynon,R.J.
  TITLE     The astacin family of metalloendopeptidases
  JOURNAL   J Biol Chem 266 (32), 21381-21385 (1991)
   PUBMED   1939172
REFERENCE   8  (bases 1 to 2923)
  AUTHORS   Gorbea,C.M., Flannery,A.V. and Bond,J.S.
  TITLE     Homo- and heterotetrameric forms of the membrane-bound
            metalloendopeptidases meprin A and B
  JOURNAL   Arch Biochem Biophys 290 (2), 549-553 (1991)
   PUBMED   1929422
REFERENCE   9  (bases 1 to 2923)
  AUTHORS   Wolz,R.L., Harris,R.B. and Bond,J.S.
  TITLE     Mapping the active site of meprin-A with peptide substrates and
            inhibitors
  JOURNAL   Biochemistry 30 (34), 8488-8493 (1991)
   PUBMED   1883833
REFERENCE   10 (bases 1 to 2923)
  AUTHORS   Craig,S.S., Mader,C. and Bond,J.S.
  TITLE     Immunohistochemical localization of the metalloproteinase meprin in
            salivary glands of male and female mice
  JOURNAL   J Histochem Cytochem 39 (1), 123-129 (1991)
   PUBMED   1701182
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT010585.12.
            
            On Jan 22, 2019 this sequence version replaced NM_008585.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC015258.1, AK143906.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-121               CT010585.12        114928-115048       c
            122-146             CT010585.12        114816-114840       c
            147-197             CT010585.12        114663-114713       c
            198-238             CT010585.12        111666-111706       c
            239-314             CT010585.12        110175-110250       c
            315-432             CT010585.12        107134-107251       c
            433-608             CT010585.12        103855-104030       c
            609-830             CT010585.12        99364-99585         c
            831-980             CT010585.12        98486-98635         c
            981-1196            CT010585.12        94446-94661         c
            1197-1667           CT010585.12        91081-91551         c
            1668-1841           CT010585.12        90373-90546         c
            1842-2142           CT010585.12        89352-89652         c
            2143-2923           CT010585.12        86604-87384         c
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 19.73 cM"
     gene            1..2923
                     /gene="Mep1a"
                     /gene_synonym="Mep-1; Mep-1a; Mep1"
                     /note="meprin 1 alpha"
                     /db_xref="GeneID:17287"
                     /db_xref="MGI:MGI:96963"
     exon            1..121
                     /gene="Mep1a"
                     /gene_synonym="Mep-1; Mep-1a; Mep1"
                     /inference="alignment:Splign:2.1.0"
     CDS             17..2299
                     /gene="Mep1a"
                     /gene_synonym="Mep-1; Mep-1a; Mep1"
                     /EC_number="3.4.24.18"
                     /note="meprin A subunit alpha; endopeptidase-2; meprin A
                     alpha-subunit; meprin alpha"
                     /codon_start=1
                     /product="meprin A subunit alpha precursor"
                     /protein_id="NP_032611.2"
                     /db_xref="CCDS:CCDS37623.1"
                     /db_xref="GeneID:17287"
                     /db_xref="MGI:MGI:96963"
                     /translation="MARRLGRSSSFAIMLWIQPACLLSLIFSAHIAAVSIKHLLNGSD
                     HDTDVGEQKDIFEINLAAGLNLFQGDILLPRTRNAMRDPSSRWKLPIPYILADNLELN
                     AKGAILHAFEMFRLKSCVDFKPYEGESSYIIFQKLSGCWSMIGDQQVGQNISIGEGCD
                     FKATIEHEILHALGFFHEQSRTDRDDYVNIWWDQIITDYEHNFNTYDDNTITDLNTPY
                     DYESLMHYGPFSFNKNESIPTITTKIPEFNTIIGQLPDFSAIDLIRLNRMYNCTATHT
                     LLDHCDFEKTNVCGMIQGTRDDADWAHGDSSQPEQVDHTLVGQCKGAGYFMFFNTSLG
                     ARGEAALLESRILYPKRKQQCLQFFYKMTGSPADRFEVWVRRDDNAGKVRQLAKIQTF
                     QGDSDHNWKIAHVTLNEEKKFRYVFLGTKGDPGNSSGGIYLDDITLTETPCPAGVWTI
                     RNISQILENTVKGDKLVSPRFYNSEGYGVGVTLYPNGRITSNSGFLGLTFHLYSGDND
                     AILEWPVENRQAIMTILDQEADTRNRMSLTLMFTTSKNQTSSAINGSVIWDRPSKVGV
                     YDKDCDCFRSLDWGWGQAISHQLLKRRNFLKGDSLIIFVDFKDLTHLNRTEVPASARS
                     TMPRGLLLQGQESPALGESSRKAMLEESLPSSLGQRHPSRQKRSVENTGPMEDHNWPQ
                     YFRDPCDPNPCQNEGTCVNVKGMASCRCVSGHAFFYAGERCQAMHVHGSLLGLLIGCI
                     AGLIFLTFVTFSTTNGKLRQ"
     exon            122..146
                     /gene="Mep1a"
                     /gene_synonym="Mep-1; Mep-1a; Mep1"
                     /inference="alignment:Splign:2.1.0"
     exon            147..197
                     /gene="Mep1a"
                     /gene_synonym="Mep-1; Mep-1a; Mep1"
                     /inference="alignment:Splign:2.1.0"
     exon            198..238
                     /gene="Mep1a"
                     /gene_synonym="Mep-1; Mep-1a; Mep1"
                     /inference="alignment:Splign:2.1.0"
     exon            239..314
                     /gene="Mep1a"
                     /gene_synonym="Mep-1; Mep-1a; Mep1"
                     /inference="alignment:Splign:2.1.0"
     exon            315..432
                     /gene="Mep1a"
                     /gene_synonym="Mep-1; Mep-1a; Mep1"
                     /inference="alignment:Splign:2.1.0"
     exon            433..608
                     /gene="Mep1a"
                     /gene_synonym="Mep-1; Mep-1a; Mep1"
                     /inference="alignment:Splign:2.1.0"
     exon            609..830
                     /gene="Mep1a"
                     /gene_synonym="Mep-1; Mep-1a; Mep1"
                     /inference="alignment:Splign:2.1.0"
     exon            831..980
                     /gene="Mep1a"
                     /gene_synonym="Mep-1; Mep-1a; Mep1"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1196
                     /gene="Mep1a"
                     /gene_synonym="Mep-1; Mep-1a; Mep1"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1667
                     /gene="Mep1a"
                     /gene_synonym="Mep-1; Mep-1a; Mep1"
                     /inference="alignment:Splign:2.1.0"
     exon            1668..1841
                     /gene="Mep1a"
                     /gene_synonym="Mep-1; Mep-1a; Mep1"
                     /inference="alignment:Splign:2.1.0"
     exon            1842..2142
                     /gene="Mep1a"
                     /gene_synonym="Mep-1; Mep-1a; Mep1"
                     /inference="alignment:Splign:2.1.0"
     exon            2143..2923
                     /gene="Mep1a"
                     /gene_synonym="Mep-1; Mep-1a; Mep1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2902..2907
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mep1a"
                     /gene_synonym="Mep-1; Mep-1a; Mep1"
                     /note="hexamer: AATAAA"
     polyA_site      2923
                     /gene="Mep1a"
                     /gene_synonym="Mep-1; Mep-1a; Mep1"
                     /note="major polyA site"
ORIGIN      
        1 ggtccaatag gtttttatgg caaggaggct tggcagatcc agttcatttg caataatgct
       61 ttggatacaa cctgcgtgtc ttctctcact gattttttct gcccacatag cagctgtatc
      121 gattaagcat cttttgaatg gatctgatca cgatacagat gttggtgaac agaaggatat
      181 tttcgaaatc aatttagctg caggcctgaa cctctttcaa ggggacatcc ttctgcccag
      241 aaccagaaat gccatgcgag atccctcaag cagatggaag ctccccattc catacattct
      301 ggctgacaat ctggagctga atgccaaagg agccatcctc catgcctttg agatgttccg
      361 cctcaagtct tgtgtggatt tcaagcccta cgaaggggag agttcgtata tcatcttcca
      421 gaaactttct gggtgctggt ctatgattgg tgatcaacag gtgggacaga acatttccat
      481 tggtgaggga tgtgacttta aggccaccat tgaacatgaa atcctgcatg ctctgggatt
      541 cttccatgag cagtcaagga ctgaccggga tgattatgtg aacatttggt gggatcaaat
      601 cattacagac tatgaacata atttcaacac atatgacgac aacaccatca cagacctcaa
      661 cacaccctat gattatgagt ccctgatgca ctacgggcca ttttcgttta acaagaacga
      721 aagcatccct accattacta ccaagatccc tgagtttaac accatcattg gacaactccc
      781 ggacttcagt gccattgatc taataagact gaatcgaatg tacaactgca ccgcaacaca
      841 tactctgttg gaccactgtg actttgagaa gaccaacgtc tgtgggatga tccagggaac
      901 cagagatgat gctgattggg ctcatgggga cagcagtcag cctgaacaag tggatcacac
      961 cttggtggga cagtgtaaag gtgccggcta cttcatgttc ttcaatacca gcttgggggc
     1021 aagaggagag gcagcccttc tggagtctcg aatcctttat cccaagagga agcagcagtg
     1081 tttgcagttt ttttataaga tgactggaag ccctgcagac agatttgaag tctgggtgag
     1141 aagggacgac aacgcgggca aggtgcgaca gctggccaag atccagacct ttcaaggaga
     1201 ctctgatcac aactggaaaa ttgctcatgt gacactcaat gaagaaaaga aatttcgcta
     1261 tgttttcctg ggtaccaaag gggaccctgg taactcaagt ggtggtattt accttgatga
     1321 catcactctg acagaaaccc cctgccctgc aggggtttgg accatccgga atatctccca
     1381 gatccttgag aacacagtga aaggggacaa gctcgtgagc cctcggttct acaattctga
     1441 gggttacggt gtgggggtga ccttgtaccc caatggtaga atcaccagta actctggctt
     1501 cctgggactc acttttcact tgtacagtgg ggataacgat gcgattctgg agtggccagt
     1561 ggaaaacaga caggccataa tgaccatcct ggaccaggaa gctgatacca ggaacaggat
     1621 gtccctgacc ttgatgttca ctacctccaa gaaccaaaca tcttcagcta taaatggctc
     1681 tgtcatctgg gacaggccat ccaaggtggg agtctatgat aaggactgtg attgtttcag
     1741 aagccttgac tggggctggg gtcaggccat ctcccaccag ctgctgaaga gaagaaattt
     1801 tcttaaaggc gacagcctca tcattttcgt ggactttaaa gacctcaccc acctgaaccg
     1861 gactgaagtt ccagcttcag ccagaagcac gatgcccaga ggcctccttc tgcaaggcca
     1921 ggagtctcct gccttgggag aaagctccag aaaagccatg ctggaggaat ccctgccaag
     1981 cagcctgggc cagagacatc ccagcagaca gaagcgctcg gtggagaaca ctggccccat
     2041 ggaggaccac aactggccac agtacttcag agacccctgt gacccaaacc cttgccagaa
     2101 tgaaggcacc tgtgtgaacg taaagggaat ggccagctgc aggtgcgtct ctgggcacgc
     2161 ctttttctac gcaggggagc gctgtcaggc catgcacgtg cacggcagct tgctgggcct
     2221 gctcattggc tgcatcgctg gcttgatctt cttgaccttc gtcacattct ctaccaccaa
     2281 cggaaagctg cggcagtgag cagctacccg cggatgtcca cccagacttt ccttcctgtg
     2341 gtcagaacac tctgggggta gttttcatca aacatgttga gagggcctcc aaagccaaag
     2401 ccccaaacct cctgcggggt tctggccact ccatccctgt acagttctgc tatgcttctg
     2461 cgaattcctg gcgaatccat tgcgtcctaa caacagtcat catatttcat tataaacgct
     2521 tggcttgctt gcccttctcc ccttaaatca caaagtctag ttgggtaggg gcatgagtta
     2581 tcttcagcta ctacactgac cccagtgcta gaagcgtgac tgactggtgt attgtgggta
     2641 ctcagtaagc attaaatgaa tgaaccaata agcgagtgaa tagctgtggt ctagtaagtc
     2701 ttttgtttct acctttgtat cataaaacat atgattaagt ctatatgcac gtgtgttaga
     2761 accgaatact cagttttcac cagacacagg agtcttatcc ctcatgtcgt accttgtaaa
     2821 atcacttcag ggattcctct ggtcagctgg atttccacag catggacttt ctttcttgct
     2881 ctggttttaa ggaaaaaact taataaaacc actaacgatg caa
//
