LOCUS       NM_010790               2501 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus maternal embryonic leucine zipper kinase (Melk),
            transcript variant 1, mRNA.
ACCESSION   NM_010790
VERSION     NM_010790.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Ye,J., Deng,W., Zhong,Y., Liu,H., Guo,B., Qin,Z., Li,P., Zhong,X.
            and Wang,L.
  TITLE     MELK predicts poor prognosis and promotes metastasis in esophageal
            squamous cell carcinoma via activating the NF-kappaB pathway
  JOURNAL   Int J Oncol 61 (2) (2022)
   PUBMED   35730614
  REMARK    GeneRIF: MELK predicts poor prognosis and promotes metastasis in
            esophageal squamous cell carcinoma via activating the NFkappaB
            pathway.
REFERENCE   3  (bases 1 to 2501)
  AUTHORS   Seong,H.A., Manoharan,R. and Ha,H.
  TITLE     DAXX ameliorates metabolic dysfunction in mice with diet-induced
            obesity by activating the AMP-activated protein kinase-related
            kinase MPK38/MELK
  JOURNAL   Biochem Biophys Res Commun 572, 164-170 (2021)
   PUBMED   34365141
  REMARK    GeneRIF: DAXX ameliorates metabolic dysfunction in mice with
            diet-induced obesity by activating the AMP-activated protein
            kinase-related kinase MPK38/MELK.
REFERENCE   4  (bases 1 to 2501)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2501)
  AUTHORS   Seong,H.A. and Ha,H.
  TITLE     Ablation of AMPK-Related Kinase MPK38/MELK Leads to Male-Specific
            Obesity in Aged Mature Adult Mice
  JOURNAL   Diabetes 70 (2), 386-399 (2021)
   PUBMED   33268463
  REMARK    GeneRIF: Ablation of AMPK-Related Kinase MPK38/MELK Leads to
            Male-Specific Obesity in Aged Mature Adult Mice.
REFERENCE   6  (bases 1 to 2501)
  AUTHORS   Cuajungco,M.P., Leyne,M., Mull,J., Gill,S.P., Gusella,J.F. and
            Slaugenhaupt,S.A.
  TITLE     Cloning, characterization, and genomic structure of the mouse
            Ikbkap gene
  JOURNAL   DNA Cell Biol 20 (9), 579-586 (2001)
   PUBMED   11747609
REFERENCE   7  (bases 1 to 2501)
  AUTHORS   Oh,B., Hwang,S., McLaughlin,J., Solter,D. and Knowles,B.B.
  TITLE     Timely translation during the mouse oocyte-to-embryo transition
  JOURNAL   Development 127 (17), 3795-3803 (2000)
   PUBMED   10934024
REFERENCE   8  (bases 1 to 2501)
  AUTHORS   Heyer,B.S., Kochanowski,H. and Solter,D.
  TITLE     Expression of Melk, a new protein kinase, during early mouse
            development
  JOURNAL   Dev Dyn 215 (4), 344-351 (1999)
   PUBMED   10417823
REFERENCE   9  (bases 1 to 2501)
  AUTHORS   Gil,M., Yang,Y., Lee,Y., Choi,I. and Ha,H.
  TITLE     Cloning and expression of a cDNA encoding a novel protein
            serine/threonine kinase predominantly expressed in hematopoietic
            cells
  JOURNAL   Gene 195 (2), 295-301 (1997)
   PUBMED   9305775
REFERENCE   10 (bases 1 to 2501)
  AUTHORS   Heyer,B.S., Warsowe,J., Solter,D., Knowles,B.B. and Ackerman,S.L.
  TITLE     New member of the Snf1/AMPK kinase family, Melk, is expressed in
            the mouse egg and preimplantation embryo
  JOURNAL   Mol Reprod Dev 47 (2), 148-156 (1997)
   PUBMED   9136115
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL805952.11 and AL807399.5.
            
            On Mar 27, 2023 this sequence version replaced NM_010790.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X95351.1, AK011932.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-122               AL805952.11        159362-159483
            123-218             AL805952.11        161982-162077
            219-304             AL805952.11        164443-164528
            305-421             AL805952.11        165401-165517
            422-565             AL805952.11        167317-167460
            566-634             AL805952.11        168701-168769
            635-727             AL805952.11        170575-170667
            728-826             AL805952.11        176435-176533
            827-895             AL805952.11        182611-182679
            896-994             AL805952.11        184052-184150
            995-1081            AL805952.11        191291-191377
            1082-1201           AL805952.11        199037-199156
            1202-1312           AL807399.5         5784-5894
            1313-1544           AL807399.5         7995-8226
            1545-1641           AL807399.5         10715-10811
            1642-1810           AL807399.5         11813-11981
            1811-1914           AL807399.5         21743-21846
            1915-2501           AL807399.5         24581-25167
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 23.46 cM"
     gene            1..2501
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /note="maternal embryonic leucine zipper kinase"
                     /db_xref="GeneID:17279"
                     /db_xref="MGI:MGI:106924"
     exon            1..122
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            123..218
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /inference="alignment:Splign:2.1.0"
     CDS             161..2092
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.10.2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein kinase PK38; tyrosine-protein kinase MELK"
                     /codon_start=1
                     /product="maternal embryonic leucine zipper kinase isoform
                     1"
                     /protein_id="NP_034920.2"
                     /db_xref="CCDS:CCDS18124.1"
                     /db_xref="GeneID:17279"
                     /db_xref="MGI:MGI:106924"
                     /translation="MKDYDELLKYYELYETIGTGGFAKVKLACHVLTGEMVAIKIMDK
                     NALGSDLPRVKTEIDALKSLRHQHICQLYHVLETKNKIFMVLEYCPGGELFDYIISQD
                     RLSEEETRVVFRQILSAVAYVHSQGYAHRDLKPENLLFDENHKLKLIDFGLCAKPKGN
                     KDYHLQTCCGSLAYAAPELIQGKSYLGSEADVWSMGILLYVLMCGFLPFDDDNVMALY
                     KKIMRGKYEVPKWLSPSSILLLQQMLQVDPKKRISMRNLLNHPWVMQDYSCPVEWQSK
                     TPLTHLDEDCVTELSVHHRSSRQTMEDLISSWQYDHLTATYLLLLAKKARGKPARLQL
                     LSFSCGTASTTPKSKNLSLEDMSTSDDNCVAGLIDYELCEDKLLAPKTPQVTKHLAES
                     NHAASKSPAPGVRRAVANKLMDKENVCTPKSSVKNEEQFVFSEPKIPVSKNQYKREIP
                     ASPTRFPTPAKARAQCLREAPVRTPGNSAGADTLTTGVISPERRCRSMDVDLNQAHME
                     DTPKKKGTNVFGSLERGLDKVLTALTRNKKKGSARDGPRKRKLHYNVTTTRLVNPDQL
                     LSEIMAILPKKNVDFVQKGYTLKCQTQSDFGKVTMQFELEVCQLQRPDVVGIRRQRLK
                     GDAWVYKRLVEDILSGCKM"
     exon            219..304
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            305..421
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            422..565
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            566..634
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            635..727
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            728..826
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            827..895
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            896..994
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1081
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1201
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1312
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            1313..1544
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            1545..1641
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            1642..1810
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            1811..1914
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /inference="alignment:Splign:2.1.0"
     exon            1915..2501
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2470..2475
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /note="hexamer: AATAAA"
     polyA_site      2501
                     /gene="Melk"
                     /gene_synonym="mKIAA0175; MPK38"
                     /note="major polyA site"
ORIGIN      
        1 aacttgaatc ctgcagaaat tcccaccgac ttgttccggc tttgctagac tctgcagccg
       61 gctgcttcac cttctgtcct gttcggtcct gttcggtcct tgctgggccc gctgcccttc
      121 aggttctggt tgtcattttg aataaccttc caggtggact atgaaagatt atgacgaact
      181 cctcaaatac tatgaactat atgaaacgat tgggacaggt ggctttgcaa aggtcaaact
      241 ggcctgccat gtcctcactg gagagatggt agctataaaa atcatggata agaatgcgct
      301 agggagtgat ttgccccgag tcaaaactga gatcgatgcg ctgaagagtc tgagacatca
      361 gcacatatgt cagctctacc atgtgctgga gacaaagaac aaaatattca tggttctgga
      421 gtactgtcca ggaggagagc tgtttgacta cataatctcc caggatcgcc tgtcggaaga
      481 ggagacccgg gtcgtcttcc gtcagatact gtctgcagtt gcgtatgtcc acagccaggg
      541 ctatgcccac agggacctca aaccagaaaa tttattattt gatgaaaatc ataagctaaa
      601 gctgattgac tttggtcttt gtgcaaaacc caagggcaac aaggactacc atctgcagac
      661 gtgctgtggg agccttgctt atgcagctcc tgaactaata caagggaagt cgtaccttgg
      721 atcagaggca gatgtttgga gcatgggcat cctcctgtat gtgctcatgt gtggatttct
      781 accatttgat gatgataatg tcatggcttt gtacaagaag ataatgagag ggaaatacga
      841 agttcctaag tggctctctc ccagtagcat tctgcttctc cagcagatgt tgcaggtgga
      901 cccaaagaaa cggatttcta tgagaaatct cctgaaccat ccctgggtca tgcaagatta
      961 cagctgtccc gtggagtggc aaagcaagac tcctttgact cacctcgatg aggattgcgt
     1021 gacagagctt tctgtacatc accgcagcag caggcagaca atggaggatt taatttcgtc
     1081 gtggcagtac gatcacctca cagccaccta ccttctgctt ctagccaaga aggcccgggg
     1141 gaagccggct cgtctacagc tcctgtcctt ctcttgtgga accgccagca ccactccaaa
     1201 gtcaaagaat ctgagcctgg aagatatgag cacaagtgat gataactgtg tggctggatt
     1261 gatagactat gaattgtgtg aagataaatt attagctccc aagacgccac aggttaccaa
     1321 acacttggca gaatcaaatc acgcagcatc taaatcacca gcgccagggg tacgcagagc
     1381 agtggcaaat aaattaatgg acaaagaaaa tgtgtgcact cccaagtctt ctgtgaagaa
     1441 tgaagagcag tttgtatttt ctgagccgaa gattccagtt agtaagaacc agtataagag
     1501 agaaataccc gcctcaccaa cccgtttccc aacacctgca aaagctagag cccagtgcct
     1561 gagagaagcc ccggttagaa caccagggaa ttccgcagga gcagacacac taacgacagg
     1621 tgtcattagc cccgagagga ggtgccgttc aatggacgtg gatctcaacc aggcacacat
     1681 ggaggatacc ccgaaaaaga aaggaaccaa tgtgtttggg agccttgaga gaggactgga
     1741 taaggttctc actgcgctca caaggaacaa gaagaagggc tctgccagag atggaccaag
     1801 aaagcgaaag ctgcactaca atgtgactac aactcgcctg gtgaaccccg accagctcct
     1861 gagcgaaatc atggctattc ttccaaagaa gaacgtggac ttcgtacaga aaggttacac
     1921 tctaaagtgt caaacgcagt ctgattttgg caaagtgaca atgcagtttg aactggaagt
     1981 gtgccagctg cagagacctg acgtggtagg catccggaga cagcggctga agggtgatgc
     2041 ctgggtttac aagagattag tggaagatat cttgtctggc tgcaagatgt gaccgatgac
     2101 agtgtccgtc tgcccggatg aatgtgggtg tgatgttgca cacaggaaga ctgtgagatt
     2161 ggttccattt gggggccatt ggatctatgg aactacaagc tcatttaaat agccatcttc
     2221 aagaccaatg tatttttgct ttttaaacaa aaatttttac tttgtggctg aatccaaggc
     2281 aactgttctg tcattatcaa ttactctctg ttttaatcat gggcttggta tgttagtaac
     2341 tgtttttata ttcacttcca tcaggggatg ccagctcttc actgtgactc attttgatgt
     2401 acagtttctt tctgaactaa aaccatttgt gaatatatca agctcttttt tgtatctgat
     2461 tttgatccaa ataaaacctc gaatggcttc ctgactgtta a
//
