LOCUS       NM_029197               1703 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus meiosis specific with OB domains (Meiob), mRNA.
ACCESSION   NM_029197 XM_128478 XM_914580
VERSION     NM_029197.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Guan,Y., Lin,H., Leu,N.A., Ruthel,G., Fuchs,S.Y., Busino,L., Luo,M.
            and Wang,P.J.
  TITLE     SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early
            meiotic recombination
  JOURNAL   Nucleic Acids Res 50 (9), 5129-5144 (2022)
   PUBMED   35489071
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Guo,R., Xu,Y., Leu,N.A., Zhang,L., Fuchs,S.Y., Ye,L. and Wang,P.J.
  TITLE     The ssDNA-binding protein MEIOB acts as a dosage-sensitive
            regulator of meiotic recombination
  JOURNAL   Nucleic Acids Res 48 (21), 12219-12233 (2020)
   PUBMED   33166385
  REMARK    GeneRIF: The ssDNA-binding protein MEIOB acts as a dosage-sensitive
            regulator of meiotic recombination.
            GeneRIF: Two mutations (N64I and S67 deletion) in MEIOB cause
            self-aggregation of MEIOB and sharply reduced protein half-life.
            In addition to a role in early meiotic recombination, MEIOB/SPATA22
            plays a role in late meiotic recombination, i.e., in crossover
            formation, in a dosage-dependent manner.
REFERENCE   3  (bases 1 to 1703)
  AUTHORS   Xu,Y., Liu,R., Leu,N.A., Zhang,L., Ibragmova,I., Schultz,D.C. and
            Wang,P.J.
  TITLE     A cell-based high-content screen identifies isocotoin as a small
            molecule inhibitor of the meiosis-specific MEIOB-SPATA22
            complexdagger
  JOURNAL   Biol Reprod 103 (2), 333-342 (2020)
   PUBMED   32463099
  REMARK    GeneRIF: Aspartic acid 383 (D383) in MEIOB is essential for meiosis
            in mice. Isocotoin, a small molecule, inhibits the MEIOB-SPATA22
            interaction and promotes their degradation in HEK293 cells in a
            dose-dependent manner.
REFERENCE   4  (bases 1 to 1703)
  AUTHORS   Zhang,J., Gurusaran,M., Fujiwara,Y., Zhang,K., Echbarthi,M.,
            Vorontsov,E., Guo,R., Pendlebury,D.F., Alam,I., Livera,G.,
            Emmanuelle,M., Wang,P.J., Nandakumar,J., Davies,O.R. and Shibuya,H.
  TITLE     The BRCA2-MEILB2-BRME1 complex governs meiotic recombination and
            impairs the mitotic BRCA2-RAD51 function in cancer cells
  JOURNAL   Nat Commun 11 (1), 2055 (2020)
   PUBMED   32345962
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1703)
  AUTHORS   Shi,B., Xue,J., Zhou,J., Kasowitz,S.D., Zhang,Y., Liang,G.,
            Guan,Y., Shi,Q., Liu,M., Sha,J., Huang,X. and Wang,P.J.
  TITLE     MORC2B is essential for meiotic progression and fertility
  JOURNAL   PLoS Genet 14 (1), e1007175 (2018)
   PUBMED   29329290
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1703)
  AUTHORS   Xu,Y., Greenberg,R.A., Schonbrunn,E. and Wang,P.J.
  TITLE     Meiosis-specific proteins MEIOB and SPATA22 cooperatively associate
            with the single-stranded DNA-binding replication protein A complex
            and DNA double-strand breaks
  JOURNAL   Biol Reprod 96 (5), 1096-1104 (2017)
   PUBMED   28453612
  REMARK    GeneRIF: We find that MEIOB and SPATA22 form an obligate complex
            and contain defined interaction domains
            GeneRIF: MEIOB and SPATA22 form an obligate complex and contain
            defined interaction domains.  A single residue D383 in MEIOB is
            indispensable for the MEIOB-SPATA22 interaction.  The MEIOB/SPATA22
            complex interacts with the RPA heterotrimeric complex in a
            collaborative manner.
REFERENCE   7  (bases 1 to 1703)
  AUTHORS   Abby,E., Tourpin,S., Ribeiro,J., Daniel,K., Messiaen,S., Moison,D.,
            Guerquin,J., Gaillard,J.C., Armengaud,J., Langa,F., Toth,A.,
            Martini,E. and Livera,G.
  TITLE     Implementation of meiosis prophase I programme requires a conserved
            retinoid-independent stabilizer of meiotic transcripts
  JOURNAL   Nat Commun 7, 10324 (2016)
   PUBMED   26742488
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 1703)
  AUTHORS   Luo,M., Yang,F., Leu,N.A., Landaiche,J., Handel,M.A., Benavente,R.,
            La Salle,S. and Wang,P.J.
  TITLE     MEIOB exhibits single-stranded DNA-binding and exonuclease
            activities and is essential for meiotic recombination
  JOURNAL   Nat Commun 4, 2788 (2013)
   PUBMED   24240703
  REMARK    GeneRIF: MEIOB is required for meiotic recombination and
            chromosomal synapsis.
            GeneRIF: MEIOB is a meiosis-specific paralogue of RPA1.  MEIOB
            colocalizes with RPA in foci on meiotic chromosomes.  MEIOB binds
            to single-stranded DNA and exhibits 3'-5' exonuclease activity.
            MEIOB forms a complex with RPA and with SPATA22.  Chromatin
            localization and stability of MEIOB depends on SPATA22 and vice
            versa.  MEIOB is essential for meiotic recombination in both sexes.
REFERENCE   9  (bases 1 to 1703)
  AUTHORS   Souquet,B., Abby,E., Herve,R., Finsterbusch,F., Tourpin,S., Le
            Bouffant,R., Duquenne,C., Messiaen,S., Martini,E.,
            Bernardino-Sgherri,J., Toth,A., Habert,R. and Livera,G.
  TITLE     MEIOB targets single-strand DNA and is necessary for meiotic
            recombination
  JOURNAL   PLoS Genet 9 (9), e1003784 (2013)
   PUBMED   24068956
REFERENCE   10 (bases 1 to 1703)
  AUTHORS   Kogo,H., Kowa-Sugiyama,H., Yamada,K., Bolor,H., Tsutsumi,M.,
            Ohye,T., Inagaki,H., Taniguchi,M., Toda,T. and Kurahashi,H.
  TITLE     Screening of genes involved in chromosome segregation during
            meiosis I: toward the identification of genes responsible for
            infertility in humans
  JOURNAL   J Hum Genet 55 (5), 293-299 (2010)
   PUBMED   20339383
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC166102.2.
            
            On or before Jul 25, 2009 this sequence version replaced
            XM_914580.3, XM_128478.6.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189685.161270.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                AC166102.2         137926-137956       c
            32-109              AC166102.2         129257-129334       c
            110-167             AC166102.2         125749-125806       c
            168-299             AC166102.2         123930-124061       c
            300-372             AC166102.2         122394-122466       c
            373-568             AC166102.2         118611-118806       c
            569-669             AC166102.2         114306-114406       c
            670-722             AC166102.2         113334-113386       c
            723-818             AC166102.2         113153-113248       c
            819-917             AC166102.2         109815-109913       c
            918-1071            AC166102.2         108290-108443       c
            1072-1255           AC166102.2         107163-107346       c
            1256-1342           AC166102.2         105816-105902       c
            1343-1703           AC166102.2         102551-102911       c
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 12.53 cM"
     gene            1..1703
                     /gene="Meiob"
                     /gene_synonym="4930528F23Rik; MLZ-675"
                     /note="meiosis specific with OB domains"
                     /db_xref="GeneID:75178"
                     /db_xref="MGI:MGI:1922428"
     exon            1..31
                     /gene="Meiob"
                     /gene_synonym="4930528F23Rik; MLZ-675"
                     /inference="alignment:Splign:2.1.0"
     exon            32..109
                     /gene="Meiob"
                     /gene_synonym="4930528F23Rik; MLZ-675"
                     /inference="alignment:Splign:2.1.0"
     CDS             41..1453
                     /gene="Meiob"
                     /gene_synonym="4930528F23Rik; MLZ-675"
                     /note="protein expressed in male leptotene and zygotene
                     spermatocytes 675"
                     /codon_start=1
                     /product="meiosis-specific with OB domain-containing
                     protein"
                     /protein_id="NP_083473.1"
                     /db_xref="CCDS:CCDS50020.1"
                     /db_xref="GeneID:75178"
                     /db_xref="MGI:MGI:1922428"
                     /translation="MAKFFALKNFTALSDLHPNMANLKIIGIVIGKTDVKGFPDRKNI
                     GSERYTFSFTIRDSPCHFVNVSSWGSEDYIRSLSESFRVAECVIIENPLIQRKDTERE
                     EKFSPATPSNYKLLLSENHSMVKVCSPYEVDTKLLSLIHLPVKESRDYYSLADIVANG
                     HSLDGRIINVLAAVRSVGEPKYFTTSDRRKGQRCEVKLFDETEPSFTMTCWDNESILL
                     AQSWMARETVIFASDVRINFNKFQNCMAATVISKTIITVNPDTPEANILLNYIRENKE
                     TNVADEIDSYLKESVNLNTIVDVYTVEQLKVKALKSEGKADPFYGILYAYISTLNIDD
                     ETTKVVRNRCSSCGYIVNEASNTCTICNQDSSRLKSFFLSFDVLVDLTDHTGTLHSCS
                     LSGSIAEETLGCTINEFLTMTSEQKTKLKWQLLLERSKIYLKLILSHRARGGLKVTIL
                     SCKLADPTEASRNLARQGHT"
     exon            110..167
                     /gene="Meiob"
                     /gene_synonym="4930528F23Rik; MLZ-675"
                     /inference="alignment:Splign:2.1.0"
     exon            168..299
                     /gene="Meiob"
                     /gene_synonym="4930528F23Rik; MLZ-675"
                     /inference="alignment:Splign:2.1.0"
     exon            300..372
                     /gene="Meiob"
                     /gene_synonym="4930528F23Rik; MLZ-675"
                     /inference="alignment:Splign:2.1.0"
     exon            373..568
                     /gene="Meiob"
                     /gene_synonym="4930528F23Rik; MLZ-675"
                     /inference="alignment:Splign:2.1.0"
     exon            569..669
                     /gene="Meiob"
                     /gene_synonym="4930528F23Rik; MLZ-675"
                     /inference="alignment:Splign:2.1.0"
     exon            670..722
                     /gene="Meiob"
                     /gene_synonym="4930528F23Rik; MLZ-675"
                     /inference="alignment:Splign:2.1.0"
     exon            723..818
                     /gene="Meiob"
                     /gene_synonym="4930528F23Rik; MLZ-675"
                     /inference="alignment:Splign:2.1.0"
     exon            819..917
                     /gene="Meiob"
                     /gene_synonym="4930528F23Rik; MLZ-675"
                     /inference="alignment:Splign:2.1.0"
     exon            918..1071
                     /gene="Meiob"
                     /gene_synonym="4930528F23Rik; MLZ-675"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1255
                     /gene="Meiob"
                     /gene_synonym="4930528F23Rik; MLZ-675"
                     /inference="alignment:Splign:2.1.0"
     exon            1256..1342
                     /gene="Meiob"
                     /gene_synonym="4930528F23Rik; MLZ-675"
                     /inference="alignment:Splign:2.1.0"
     exon            1343..1703
                     /gene="Meiob"
                     /gene_synonym="4930528F23Rik; MLZ-675"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtgggtcacc tagcgggccc ggaagtatcc gatttaaagc atggcaaagt tctttgcatt
       61 gaagaacttc actgcacttt ccgatcttca tccaaatatg gctaatctga aaataatcgg
      121 aatagttatt gggaaaacag atgtcaaagg ctttccagac agaaaaaata ttggatcaga
      181 aagatacact ttcagtttca ccattcgtga ctcaccatgt cactttgtga atgtatcctc
      241 ctggggcagt gaggactaca tccgctcgct gtccgagagc ttcagggttg ctgagtgtgt
      301 gataattgaa aatcccctga tccaaagaaa ggacacagaa agagaagaaa agtttagccc
      361 tgcaactcct agcaactata aactattgct cagtgagaat cactcaatgg taaaagtttg
      421 ctccccttat gaagtggata ccaagttact ttctttgata cacttgcctg ttaaagaatc
      481 tcgagattat tattcattgg ctgacattgt tgcaaatgga cacagtcttg atgggaggat
      541 tattaatgtg cttgcagctg tgagatcggt tggagaacca aaatacttta caacttcaga
      601 ccgaagaaaa ggccagagat gtgaggttaa actctttgat gaaacagagc cttctttcac
      661 aatgacatgt tgggataatg aatccattct acttgcacag agctggatgg cgcgagaaac
      721 agtaatattt gcctctgatg tgagaataaa ttttaacaaa tttcagaact gcatggcagc
      781 aactgtaatc tcaaagacca ttatcacagt gaatccagat acaccagaag ccaatatcct
      841 actgaattat ataagagaaa ataaagagac aaatgtagct gatgaaattg acagttactt
      901 gaaagaatcc gtaaacttaa acacaatagt tgatgtctat acagtggaac agctaaaagt
      961 aaaagctttg aaaagtgaag gaaaagctga tcctttctat ggcatccttt atgcttacat
     1021 ttctacattg aacattgatg acgaaactac caaagtagtt agaaatagat gttccagctg
     1081 tggttatatt gtaaatgaag cctccaatac ttgtacaatt tgcaaccaag attcttccag
     1141 attgaaatct ttttttctca gttttgatgt gctagttgat cttactgacc acactggaac
     1201 cctccattcc tgcagtctct caggaagtat tgctgaggaa actttgggct gcacgataaa
     1261 tgagtttctc acaatgacaa gtgaacagaa aacaaagctg aagtggcaac ttcttttgga
     1321 aagaagcaaa atttatttaa aattgatttt atcacacaga gcaaggggtg gactgaaagt
     1381 caccatactc tcctgtaagc ttgcagatcc tactgaggca agcaggaacc tggctagaca
     1441 aggacatact taaaaccaga ttttgctcca aattctggga aaatatagac atttaactct
     1501 tacgatggaa tgttagcttg agaattatgt gtaaaattac attttgccta aatgtggtaa
     1561 aattatttct tagattgtca ctgtttcctt ctagagttag ctcagccccc tggaatacca
     1621 tttctattaa ccttaccctg tctactctca catggggaaa tgtttgctta tgtacagaaa
     1681 taaacatgct tggtcttcct ccc
//
