LOCUS       NM_175213               4827 bp    mRNA    linear   ROD 29-OCT-2024
DEFINITION  Mus musculus meiotic double-stranded break formation protein 4
            (Mei4), transcript variant 1, mRNA.
ACCESSION   NM_175213
VERSION     NM_175213.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Pan,Z., Wang,W., Wu,L., Yao,Z., Wang,W., Chen,Y., Gu,H., Dong,J.,
            Mu,J., Zhang,Z., Fu,J., Li,Q., Wang,L., Sun,X., Kuang,Y., Sang,Q.
            and Chen,B.
  TITLE     Bi-allelic missense variants in MEI4 cause preimplantation
            embryonic arrest and female infertility
  JOURNAL   Hum Genet 143 (9-10), 1049-1060 (2024)
   PUBMED   38252283
  REMARK    GeneRIF: Bi-allelic missense variants in MEI4 cause preimplantation
            embryonic arrest and female infertility.
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Biot,M., Toth,A., Brun,C., Guichard,L., de Massy,B. and Grey,C.
  TITLE     Principles of chromosome organization for meiotic recombination
  JOURNAL   Mol Cell 84 (10), 1826-1841 (2024)
   PUBMED   38657614
REFERENCE   3  (bases 1 to 4827)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 4827)
  AUTHORS   Laroussi,H., Juarez-Martinez,A.B., Le Roy,A., Boeri Erba,E.,
            Gabel,F., de Massy,B. and Kadlec,J.
  TITLE     Characterization of the REC114-MEI4-IHO1 complex regulating meiotic
            DNA double-strand break formation
  JOURNAL   EMBO J 42 (16), e113866 (2023)
   PUBMED   37431931
  REMARK    GeneRIF: Characterization of the REC114-MEI4-IHO1 complex
            regulating meiotic DNA double-strand break formation.
REFERENCE   5  (bases 1 to 4827)
  AUTHORS   Guan,Y., Lin,H., Leu,N.A., Ruthel,G., Fuchs,S.Y., Busino,L., Luo,M.
            and Wang,P.J.
  TITLE     SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early
            meiotic recombination
  JOURNAL   Nucleic Acids Res 50 (9), 5129-5144 (2022)
   PUBMED   35489071
REFERENCE   6  (bases 1 to 4827)
  AUTHORS   Stanzione,M., Baumann,M., Papanikos,F., Dereli,I., Lange,J.,
            Ramlal,A., Trankner,D., Shibuya,H., de Massy,B., Watanabe,Y.,
            Jasin,M., Keeney,S. and Toth,A.
  TITLE     Meiotic DNA break formation requires the unsynapsed chromosome
            axis-binding protein IHO1 (CCDC36) in mice
  JOURNAL   Nat Cell Biol 18 (11), 1208-1220 (2016)
   PUBMED   27723721
REFERENCE   7  (bases 1 to 4827)
  AUTHORS   Kato,Y., Katsuki,T., Kokubo,H., Masuda,A. and Saga,Y.
  TITLE     Dazl is a target RNA suppressed by mammalian NANOS2 in sexually
            differentiating male germ cells
  JOURNAL   Nat Commun 7, 11272 (2016)
   PUBMED   27072294
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 4827)
  AUTHORS   Robert,T., Nore,A., Brun,C., Maffre,C., Crimi,B., Bourbon,H.M. and
            de Massy,B.
  TITLE     The TopoVIB-Like protein family is required for meiotic DNA
            double-strand break formation
  JOURNAL   Science 351 (6276), 943-949 (2016)
   PUBMED   26917764
  REMARK    Erratum:[Science. 2016 May 6;352(6286):aaf9649. doi:
            10.1126/science.aaf9649. PMID: 27151875]
REFERENCE   9  (bases 1 to 4827)
  AUTHORS   Kumar,R., Ghyselinck,N., Ishiguro,K., Watanabe,Y., Kouznetsova,A.,
            Hoog,C., Strong,E., Schimenti,J., Daniel,K., Toth,A. and de
            Massy,B.
  TITLE     MEI4 - a central player in the regulation of meiotic DNA
            double-strand break formation in the mouse
  JOURNAL   J Cell Sci 128 (9), 1800-1811 (2015)
   PUBMED   25795304
  REMARK    GeneRIF: the association of MEI4 to chromosome axes is required for
            DNA double-strand break formation.
REFERENCE   10 (bases 1 to 4827)
  AUTHORS   Kumar,R., Bourbon,H.M. and de Massy,B.
  TITLE     Functional conservation of Mei4 for meiotic DNA double-strand break
            formation from yeasts to mice
  JOURNAL   Genes Dev 24 (12), 1266-1280 (2010)
   PUBMED   20551173
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC163294.2, AK043929.1 and AC122931.4.
            
            On Oct 28, 2017 this sequence version replaced NM_175213.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK043929.1, BC096613.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849383, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-74                AC163294.2         202277-202350
            75-2926             AK043929.1         2-2853
            2927-4827           AC122931.4         80371-82271         c
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 44.72 cM"
     gene            1..4827
                     /gene="Mei4"
                     /gene_synonym="4930486G11Rik"
                     /note="meiotic double-stranded break formation protein 4"
                     /db_xref="GeneID:75033"
                     /db_xref="MGI:MGI:1922283"
     exon            1..248
                     /gene="Mei4"
                     /gene_synonym="4930486G11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            249..494
                     /gene="Mei4"
                     /gene_synonym="4930486G11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            495..1030
                     /gene="Mei4"
                     /gene_synonym="4930486G11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1031..1162
                     /gene="Mei4"
                     /gene_synonym="4930486G11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..4827
                     /gene="Mei4"
                     /gene_synonym="4930486G11Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2080..2085
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mei4"
                     /gene_synonym="4930486G11Rik"
                     /note="hexamer: AATAAA"
     regulatory      2084..2089
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mei4"
                     /gene_synonym="4930486G11Rik"
                     /note="hexamer: AATACA"
     polyA_site      2100
                     /gene="Mei4"
                     /gene_synonym="4930486G11Rik"
     polyA_site      2106
                     /gene="Mei4"
                     /gene_synonym="4930486G11Rik"
                     /note="major polyA site"
     regulatory      4679..4684
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mei4"
                     /gene_synonym="4930486G11Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      4702
                     /gene="Mei4"
                     /gene_synonym="4930486G11Rik"
ORIGIN      
        1 agaaagccgg gaggagtggc gctctgggct ctggcgccta cctgcgggct gcgcttgcat
       61 cagcgtgcat ggccgggaat actggccccc gagcttcccc cgccgcctga gggcgtcctt
      121 ccttctcagg aaccccaggc ttgtgtagca tctgcctgct ctctgtactc tcgggccccg
      181 ggtcccctca agcagcgcac gctctcaggt gctgtggctt ggggttcctt gtcgcgccct
      241 ttgcggaggg acaaaagcca agatggatat tcagccatgg tatttgaaaa cttcaaagct
      301 ggcactggcg ttggcgatta tccactcaaa accagcagac agaagcagca gagaatatac
      361 agagtacctg gcttccttgg tgacccagaa ggagtccaca tggaaatcaa aacttgaagc
      421 cttagaagca gaagttctac aattacgtca aaaacttctc ctgagtagga tttcctcagg
      481 attgtttaag aatggaccgg atgtgctccc cacgttgtca gatcaggaac ctacaagttc
      541 tgaaaataca ttgacactga tggatgactc tggatgtgtt ttatcaaatg agcaaagaaa
      601 cgaacctgca gaattatccc agcattttgt ggagagcacc gatcccccac tcctccccct
      661 gcctcttgag aaaaggccac gcacgaccct ggaaaatccc ctgtcttctc acatgcagtt
      721 ctttcaacat ctgctggagc tcaagaaatg gacagaatcc agtagtctga aagtatactt
      781 aacccacttt gaaaaagact cttccacagt ttctgattca gtttcacagt tgttagatgc
      841 cctgatcaca ttttacagga accccaaact ccccttctca agcttttgga cagaggctgt
      901 tggcacatta gccagactgg caagtgattt taatttatca aatcatattt ttaaaagatg
      961 ttctaagaag ttggaagaat ttgaaaaaac tctactccag gctattttag agaataacag
     1021 tataaaccgg ttccaggtac agcgttatgt ctctcagagc ctagtaaccc taggaagttg
     1081 cagcttgttg aggaagtcta ttatatcgct gctactatca gaagtgaaca gctttgtgga
     1141 tgacctggga gccatcgatc aggatcaagg catttatgat gtgacacgct atgaaaatat
     1201 tttctccctg ttttggattc tggaacaagt tcttcaacag gcaccgcagg gagatcgcac
     1261 tgcacacatg gatcacagca tcccagaaat gcagacattt cttcagaagc atgatgaagt
     1321 catcttccga ctttcggatg cctttccttt atttgcgttc tacttatgga gactcggtgt
     1381 tctcttgaac tcagcagaga tggaaactgt taagaatgaa tcgttgcctt agcggttttc
     1441 cacaaatcag aaaatggtgt gcttcagtat ttctcaaaaa aaaagttttt aaatgaatat
     1501 tttaaattga ttggccaatc gaatcattaa ttcaatttag tgataggtct tctatataag
     1561 agctgatgaa ccactgttcc atattacaat caggcaattt cagttgacaa tgctccctta
     1621 tgctcgtgga ttatatctta tttcactcaa cagtctagct ctagtctcta aaacaaccca
     1681 tttcataaaa tacaataact tgtcaaagat ttccatctga tgtaaatttc attacaaatt
     1741 gtaatgaaat taaacttaaa cttctgtcat tttacacact taaatacaag taaatggtaa
     1801 aaaataaaat gtattacgta gctctactca aatcattttt ctcttaagga tagtgctgta
     1861 gaaaaagaaa aggctttgga atccagtggc ttttaatttt acttaatcaa catgtttgta
     1921 aagtattttt gaagtgttaa ttagttcttt aaaaatgtcc ctttttaaaa caatttttct
     1981 gttgattata aatgctattg ttcctttgat tgtttaaatg tagtaaagtt gtgtattagt
     2041 aagtcatcag ggatgttttt ataaatttta atatcctaca ataaatacac attatactta
     2101 ttaaaagtac tggttgaatt ttttcattgc acaatttcct ttacttcatt tcagttatta
     2161 aaattgtaac agacaagctt aatgagcaca tattttgcac tttcctattg tgcagtttaa
     2221 agaaaatata atgtaattct ctttcctttg ttaattatgt ctaattctgt tttataaggt
     2281 aattatcatt ttcagatgcc attagtacta gaaaaattat ccttgctaat tctttctaca
     2341 atgtatataa aattatgcca tattctagta ttatttttgg agtttgtttt tttttttttt
     2401 taaaccagca gatctcttct ctcctagaaa tggctgtata aacaagacct gaaaatgact
     2461 atatcagtgg acatagtaac atggaagaga caaaatttca tgggctcaca ctcctagata
     2521 aagaactaaa gacaagccgg gcatggtggc acacgccttt gatcccagca ctcgggagac
     2581 agaggcaggc agatttctga gtttgaggcc agtctggtct acaaagtgag ttccaggaca
     2641 gccaggtcta cacagagaaa ccctgtctgg aaaaacaaaa ataataaaaa aaaaagaaag
     2701 actactggag aaagggagaa ttagcctgcc ccaggggtga gcccccttat ttgatatcca
     2761 gtacagagcg gttcaccctg aaaccttaca aacaaatcag actcagccag tgtatttgtg
     2821 aatatatgta tgtatgtatg tatgtatgta tgtatgtatg tatgtatatg tatgtatgta
     2881 acaataacaa agaaaaagag gctatcaact tgaaggggaa aatgttctga gggttggtgt
     2941 gaaggttact gagagagact gctgggagca aaaggtaata ttggtaatat ttcaaagata
     3001 tgtaagttac ttaatatcaa tccagaatat gatggtttgt tttgcttaaa aactcactct
     3061 gagaaaggag ttacactgtc attctgaata cccagtgtag tcagtttctg gctaatgaac
     3121 tggctttaaa ctttggaaaa aaaaattaaa actctccatt ctttacattt tatgtggggg
     3181 tggtgctgcc tttttgtccc ccccccgccc cattcagcca ggaccttgag cagccctggg
     3241 atgactggga ggacctaaca ctgatctggt cccttctacc attgtccatg accatgaagg
     3301 atttgccaca gctgtgtggg tgttttatct tgccacttga taaaaatcta agacattcac
     3361 aagagactgg gctgggaaac ttaccctctt ctatgtacag ctgtgtgact taaatagtct
     3421 ttctttcttt ttaagaataa atgaagtcta gtggagattt ccaattgatg gtatttattt
     3481 aagtggcata gataaaaata gacaatatga ctaacatttc ctgaggataa agcactctaa
     3541 gtctttaagc atgcaaagat caagatcaga catccataca tggattagag ttaattccca
     3601 agtcttagac atagaaataa ataaaacatt tgagcatagc ttatgctggc tggctccaaa
     3661 atcaaaccat tttttttttc agtcagaaga aaaatatctc tatttcatga ctggaattca
     3721 taggatgatt ctattttcca ggtatgatgt gtggatatag gtgcagatgt gttgctatgt
     3781 agatatgcat gcatgcgaat gggaaggtga gggtcatacc ttgggatcat ttctccttgg
     3841 ttgtttccag ggtgttgctt tgtttctttg tttgagaaag ggcctctcac tggcgcccga
     3901 gagctttcag ataatgtgtg tggggctggt gagcaagctc gggggagctt ctccctaggc
     3961 ccttccagtg tgtggattgt tagcacgagg cttcctggct ttttacatgg ggttgaactc
     4021 aggtggtcat gcttgtcagt taagcactgc accaactgag ctgtcttctc cattcccgtg
     4081 tttgatcttt ctaaatcatt gtatgaagta agaaaatgtc ttgtacccgt gtcacagaga
     4141 aaaaagtggg cagaattaat ttaaaatgca tccgtggtat taggggttat tatctgggtg
     4201 ttccccagag tagctttatt ttaggtatct cttgttctca tcttttcctg acatctggaa
     4261 aaggcatgtg aggcctgtca ttgccctctg cttgtcaaag ggcactacac atattttcag
     4321 ttctgccata ttgaaacagg agtccagtgt agaaggtaca gagaaaagac taaggaagtc
     4381 aagagaagac agggccttca ctaagtcatc ttacaacatc ggatgagtga ttcacatttt
     4441 tctcataata gcatttgcct tccagtttta aagtataaag aatgaaggta tctttaccat
     4501 taggagaata tgtttctgtt aaatgtgtgc ctaaatgata aggcaaaact gtgaatttaa
     4561 ctactattac tactactact acttattatt attattgttg ttgttgttgt tgttgttgtt
     4621 gttcttgttg ttattactac tactacttat tgttattatt attattatta ttattattat
     4681 taaatactgt gcagcattgg aagttctgtg ttgcctggct cgatgaaagt ctgtcagtgg
     4741 aagagtatag aacttggact taaaactgag gataccaccc tgtgtctatc atatgaataa
     4801 aacttgatca ttagtatttt taaaagg
//
