LOCUS       NM_001160400           10040 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus multiple EGF-like-domains 8 (Megf8), mRNA.
ACCESSION   NM_001160400 XM_001478642 XM_194337 XM_902363 XM_924870
VERSION     NM_001160400.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10040)
  AUTHORS   Kong,J.H., Young,C.B., Pusapati,G.V., Espinoza,F.H., Patel,C.B.,
            Beckert,F., Ho,S., Patel,B.B., Gabriel,G.C., Aravind,L.,
            Bazan,J.F., Gunn,T.M., Lo,C.W. and Rohatgi,R.
  TITLE     Gene-teratogen interactions influence the penetrance of birth
            defects by altering Hedgehog signaling strength
  JOURNAL   Development 148 (19) (2021)
   PUBMED   34486668
REFERENCE   2  (bases 1 to 10040)
  AUTHORS   Tiu,G.C., Kerr,C.H., Forester,C.M., Krishnarao,P.S.,
            Rosenblatt,H.D., Raj,N., Lantz,T.C., Zhulyn,O., Bowen,M.E.,
            Shokat,L., Attardi,L.D., Ruggero,D. and Barna,M.
  TITLE     A p53-dependent translational program directs tissue-selective
            phenotypes in a model of ribosomopathies
  JOURNAL   Dev Cell 56 (14), 2089-2102 (2021)
   PUBMED   34242585
REFERENCE   3  (bases 1 to 10040)
  AUTHORS   Kong,J.H., Young,C.B., Pusapati,G.V., Patel,C.B., Ho,S.,
            Krishnan,A., Lin,J.I., Devine,W., Moreau de Bellaing,A.,
            Athni,T.S., Aravind,L., Gunn,T.M., Lo,C.W. and Rohatgi,R.
  TITLE     A Membrane-Tethered Ubiquitination Pathway Regulates Hedgehog
            Signaling and Heart Development
  JOURNAL   Dev Cell 55 (4), 432-449 (2020)
   PUBMED   32966817
REFERENCE   4  (bases 1 to 10040)
  AUTHORS   Wang,W., Zheng,X., Song,H., Yang,J., Liu,X., Wang,Y., Zhang,M. and
            Zhang,Z.
  TITLE     Spatial and temporal deletion reveals a latent effect of Megf8 on
            the left-right patterning and heart development
  JOURNAL   Differentiation 113, 19-25 (2020)
   PUBMED   32203821
  REMARK    GeneRIF: Spatial and temporal deletion reveals a latent effect of
            Megf8 on the left-right patterning and heart development.
REFERENCE   5  (bases 1 to 10040)
  AUTHORS   Pusapati,G.V., Kong,J.H., Patel,B.B., Krishnan,A., Sagner,A.,
            Kinnebrew,M., Briscoe,J., Aravind,L. and Rohatgi,R.
  TITLE     CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity
            to the Morphogen Sonic Hedgehog
  JOURNAL   Dev Cell 44 (1), 113-129 (2018)
   PUBMED   29290584
  REMARK    GeneRIF: Megf8 attenuates signaling through the Hedgehog pathway by
            reducing levels of Smoothened at the cell surface and the primary
            cilium. Mutations in Megf8 lead to cardiac defects, limb defects,
            left-right patterning defects and craniofacial anomalies.
            Erratum:[Dev Cell. 2018 Jan 22;44(2):271. doi:
            10.1016/j.devcel.2018.01.002.. PMID: 29401421]
REFERENCE   6  (bases 1 to 10040)
  AUTHORS   Engelhard,C., Sarsfield,S., Merte,J., Wang,Q., Li,P., Beppu,H.,
            Kolodkin,A.L., Sucov,H.M. and Ginty,D.D.
  TITLE     MEGF8 is a modifier of BMP signaling in trigeminal sensory neurons
  JOURNAL   Elife 2, e01160 (2013)
   PUBMED   24052814
  REMARK    GeneRIF: Megf8 is involved in mediating BMP4 signaling and guidance
            of developing trigeminal ganglia axons.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 10040)
  AUTHORS   Merte,J., Wang,Q., Vander Kooi,C.W., Sarsfield,S., Leahy,D.J.,
            Kolodkin,A.L. and Ginty,D.D.
  TITLE     A forward genetic screen in mice identifies Sema3A(K108N), which
            binds to neuropilin-1 but cannot signal
  JOURNAL   J Neurosci 30 (16), 5767-5775 (2010)
   PUBMED   20410128
REFERENCE   8  (bases 1 to 10040)
  AUTHORS   Zhang,Z., Alpert,D., Francis,R., Chatterjee,B., Yu,Q., Tansey,T.,
            Sabol,S.L., Cui,C., Bai,Y., Koriabine,M., Yoshinaga,Y., Cheng,J.F.,
            Chen,F., Martin,J., Schackwitz,W., Gunn,T.M., Kramer,K.L., De
            Jong,P.J., Pennacchio,L.A. and Lo,C.W.
  TITLE     Massively parallel sequencing identifies the gene Megf8 with
            ENU-induced mutation causing heterotaxy
  JOURNAL   Proc Natl Acad Sci U S A 106 (9), 3219-3224 (2009)
   PUBMED   19218456
REFERENCE   9  (bases 1 to 10040)
  AUTHORS   Aune,C.N., Chatterjee,B., Zhao,X.Q., Francis,R., Bracero,L., Yu,Q.,
            Rosenthal,J., Leatherbury,L. and Lo,C.W.
  TITLE     Mouse model of heterotaxy with single ventricle spectrum of cardiac
            anomalies
  JOURNAL   Pediatr Res 63 (1), 9-14 (2008)
   PUBMED   18043505
REFERENCE   10 (bases 1 to 10040)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC156992.13 and AK122377.1.
            
            On or before May 24, 2009 this sequence version replaced
            XM_924870.2, XM_001478642.1, XM_194337.7, XM_902363.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU723517.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-530               AC156992.13        60266-60795         c
            531-694             AC156992.13        51416-51579         c
            695-901             AC156992.13        50283-50489         c
            902-1082            AC156992.13        49294-49474         c
            1083-1171           AC156992.13        49119-49207         c
            1172-1587           AC156992.13        47683-48098         c
            1588-1733           AC156992.13        47128-47273         c
            1734-1856           AC156992.13        46823-46945         c
            1857-2011           AC156992.13        44047-44201         c
            2012-2131           AC156992.13        43711-43830         c
            2132-2276           AC156992.13        43249-43393         c
            2277-2440           AC156992.13        43004-43167         c
            2441-2641           AC156992.13        39985-40185         c
            2642-2842           AC156992.13        39400-39600         c
            2843-3079           AC156992.13        38258-38494         c
            3080-3198           AC156992.13        37622-37740         c
            3199-3330           AC156992.13        37411-37542         c
            3331-3444           AC156992.13        37213-37326         c
            3445-3693           AC156992.13        36739-36987         c
            3694-3893           AC156992.13        36062-36261         c
            3894-4104           AC156992.13        35536-35746         c
            4105-4354           AC156992.13        35190-35439         c
            4355-4487           AC156992.13        34161-34293         c
            4488-4516           AC156992.13        31831-31859         c
            4517-10040          AK122377.1         5-5528
FEATURES             Location/Qualifiers
     source          1..10040
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 13.75 cM"
     gene            1..10040
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /note="multiple EGF-like-domains 8"
                     /db_xref="GeneID:269878"
                     /db_xref="MGI:MGI:2446294"
     exon            1..530
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     CDS             344..8713
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /note="EGF-like-domain, multiple 4; multiple epidermal
                     growth factor-like domains protein 8; EGF-like protein 4;
                     multiple EGF-like domains protein 8; epidermal growth
                     factor-like protein 4"
                     /codon_start=1
                     /product="multiple epidermal growth factor-like domains
                     protein 8 precursor"
                     /protein_id="NP_001153872.1"
                     /db_xref="CCDS:CCDS52148.1"
                     /db_xref="GeneID:269878"
                     /db_xref="MGI:MGI:2446294"
                     /translation="MALGGALALALALALAVLGPLSLRVLAGDCKGQRQVLREAPGFV
                     TDGAGNYSVNGNCEWLIEAPSPQHRILLDFLFLDTECTYDYLFVYDGDSPQGPLLASL
                     SGSTRPPPIEASSGKMLLHLFSDANYNLLGFNASFRFSLCPGGCQNHGQCKSPGVCVC
                     EPGWGGPDCGLQECSAYCGSHGTCASTLGPCRCEPGFLGRACDLHLWENQGAGWWHSV
                     SAGDPAFSARIGAAGAFLSPPGLLAVFGGQDLNKALGDLVLYNFSTNTWESWDLTPAP
                     AARHSHVAVAWAGLLVLMGGELANGLLTNDVWAFSPLGGGHWELLAPPASSSSGPPGL
                     AGHAAALVDDIWLYVSGGRTQHDLFSSGLFRFRLDHTSRGYWEQVIPAGGRPPAATGH
                     SMVFHAPSRTLLVHGGHRPSTARFSVRVNSTELFHVERRVWTTLKGRDGLQGPRERAF
                     HTASVLGNYMVVYGGNVHTHYQEEKCYEDGIFFYHLGCHQWVSGAELAPPGTPEGRAA
                     PPSGRYSHVAAVLGGSVLLVAGGYSGRPRGDLMAYKVPPFVFQAPALDYHLDYCSMYT
                     DHSVCSRDPECSWCQGACQAAPPPGTPSGACPAASCLGLGRLLSDCQACLAFSSPTAP
                     PRGPGALGWCVHNESCLPRPEQARCRGEQISGTVGWWGPAPVFVTSLEACVTQSFLPG
                     LHLLTFQQPPNASQPDKVSIVRSTTITLTPSPETDVSLVYRGFIHPLLPGGPGGPGAE
                     DVAVWARAQRLHVLARMARGPDTENMEEVGRWVAQQEKETRRLQRPGSDRLFPLPGRG
                     NKYAVEIRGQLNGSAGPGHSELTLLWDRTGVPGGSEISFFFLEPYRSSACTSYSSCLG
                     CLADQGCGWCLNSATCHLRQGRAHCEDDGSGESLLVLVPALCPLCEEHRDCHACTQDP
                     FCEWHQSTNRKGDAACSRRGRGRGALKNPEECPPLCSQRLTCEDCLANSSQCAWCQST
                     HTCFLFAAYLARYPHGGCRGWDDSVHSEPRCRSCGGFLTCHECLQSHECGWCGNEDNP
                     TLGRCLQGDFSGPLGGGNCSLWVGEGLGLPVALPARWAYARCPDVDECRLGLARCHPR
                     ATCLNTPLSYECHCQRGYQGDGITHCNRTCLEDCGHGVCSGPPDFTCVCDLGWTSDLP
                     PPTPAPGPPAPRCSRDCGCSFHSHCRRRGPGYCDECQDWTWGEHCERCRPGSFGNATG
                     SGGCRPCQCNGHGDPRRGHCDNLTGLCFCQDHTEGAHCQICSPGYYGDPRAGGSCFRE
                     CGGRALLTNVSSVALGSRRFGGLLPPGGGAARAGPGLSYCVWVVSATEALQPCVPGTL
                     CPPLTLTFSPDSSTPCTLSYVLAFDGFPRFLDTGVVQSDRSLIAAFCGQRRDRPLTVQ
                     ALSGLLVLHWEANGSSSWGFNASVGSARCGSGGPGSCPVPQECVPQDGAAGAGLCRCP
                     QGWAGPHCRMALCPENCNAHTGAGICNQSLGVCICAEGFGGPDCATKLDGGQLVWETL
                     MDSRLSADTASRFLHRLGHTMVEGPDATLWMFGGLGLPQGLLGNLYRYSVSERRWTQM
                     LAGAEDGGPGPSPRSFHAAAYVPAGRGAMYLLGGLTAGGVTRDFWVLNLTTLQWRQEK
                     PPQNMELPAVAGHTLTARRGLSLLLVGGYSPENGFNQQLLEYQLATGTWVSGAQSGTP
                     PTGLYGHSAVYHEATDSLYVFGGFRFHVELAAPSPELYSLHCPDRTWSLLAPSQGAKP
                     RPRLFHASALLGDTMVVLGGRSDPDEFSSDVLLYQVNCNTWLLPALTRPAFVGSPMEE
                     SVAHAVAAVGSRLYISGGFGGVALGRLLALTLPPDPCRLLPSPEACNQSGACTWCHGA
                     CLSGDQAHRLGCGVPPCSPMPRSPEECRRLRTCSECLARHPRTLQPGDGEASIPRCKW
                     CTNCPEGACIGRNGSCTSENDCRINQREVFWAGNCSEAACGAADCEQCTREGKCMWTR
                     QFKRTGETRRILSVQPTYDWTCFSHSLLNVSPMPVESSPPLPCPTPCHLLPNCTSCLA
                     SKGADGGWQHCVWSSSLQQCLSPSYLPLRCMAGGCGRLLRGPESCSLGCAQATQCALC
                     LRRPHCGWCAWGGQDGGGHCMEGGLSGPRDGLTCGRPGASWAFLSCPPEDECANGHHD
                     CNETQNCHDQPHGYECSCKTGYTMDNVTGVCRPVCAQGCVNGSCVEPDHCRCHFGFVG
                     RNCSTECRCNRHSECAGVGAQDHCLLCRNHTKGSHCEQCLPLFVGSALGGGTCRPCHA
                     FCRGNSHVCVSRKELEMARKEPEKYSLDPEEIETWVAEGPSEDEAVCVNCQNNSYGDR
                     CESCLHGYFLLDGKCTKCQCNGHADTCNEQDGTGCPCQNNTETGTCQGSSPSDRRDCY
                     KYQCAKCRESFHGSPLGGQQCYRLISVEQECCLDPTSQTNCFHEPKRRALGPGRTVLF
                     GVQPKFTNVDIRLTLDVTFGAVDLYVSTSYDTFVVRVAPDTGVHTVHIQPPPPPPPPP
                     PPADGVPRVAADLGGLGTGSGSGSPVEPRVREVWPRGLITYVTVTEPSAVLVVRSVRD
                     RLVITYPHEHHALKSSRFYLLLLGVGDPNGPGANGSADSQGLLFFRQDQAHIDLFVFF
                     SVFFSCFFLFLSLCVLLWKAKQALDQRQEQRRHLQEMTKMASRPFAKVTVCFPPDPAG
                     PAPAWKPAGLPPPAFRRSEPFLAPLLLTGAGGPWGPMGGGCCPPALPATTAGLRAGPI
                     TLEPTEDGMAGVATLLLQLPGGPHAPNGACLGSALVTLRHRLHEYCGGSGGAGGSGHG
                     GGGGRKGLLSQDNLTSMSL"
     exon            531..694
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            695..901
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            902..1082
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1171
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            1172..1587
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            1588..1733
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            1734..1856
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            1857..2011
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            2012..2131
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            2132..2276
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            2277..2440
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            2441..2641
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            2642..2842
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            2843..3079
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            3080..3198
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            3199..3330
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            3331..3444
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            3445..3693
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            3694..3893
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            3894..4104
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            4105..4354
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            4355..4487
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            4488..4735
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            4736..4846
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            4847..4965
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            4966..5173
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            5174..5354
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            5355..5518
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            5519..5663
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            5664..5895
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            5896..6019
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            6020..6233
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            6234..6448
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            6449..6656
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            6657..6816
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            6817..7009
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            7010..7180
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            7181..7311
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            7312..7444
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     exon            7445..10040
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10020..10025
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /note="hexamer: AATAAA"
     polyA_site      10037
                     /gene="Megf8"
                     /gene_synonym="b2b1702.2Clo; b2b1702Clo; b2b288Clo; Egfl4;
                     m687Ddg; mKIAA0817"
                     /note="major polyA site"
ORIGIN      
        1 cttgcagcag cctgaatgta atcactggat accgtttatg gagtcggcgc ccctatgggg
       61 gctcgtgtct ataggggacc cttacggtcc ctaggggtca gccctatcca taacgacttc
      121 atatccaggg ccttccatgg gtctataggg ccggcccctc ggtttcaaga gcgcatcaac
      181 tgcggccctt tccttggccc ggactgcgtg gctggtacat ccttggacta caaggccatg
      241 ctacgtctgt aaaggttgca tcttgcaggg cctcattccc agagggtctt ctccaggtct
      301 tcacggccct tcccgtccct tagggccagt gcctaaggcg gcgatggcct tgggtggagc
      361 tctggccttg gcccttgcac tggccttggc agtgctggga ccgctgtccc tcagggtcct
      421 ggctggtgac tgcaaggggc agcggcaggt gctacgggag gcgccaggct tcgtgacgga
      481 tggtgcgggc aactacagcg tcaacggcaa ctgcgagtgg cttatcgagg ccccgagccc
      541 tcagcaccgg atcctgctgg acttcctttt cctggacacg gagtgcactt atgactacct
      601 ctttgtgtat gacggtgact cgccccaagg ccctctgctt gctagtctga gtgggagcac
      661 ccggccccca cccattgagg cttcctcagg caagatgctc ctccacctct tcagcgatgc
      721 caactacaac ctgctgggct ttaacgcctc cttccgcttc tccctgtgtc ccggaggctg
      781 ccagaaccat gggcagtgca agtccccggg agtgtgcgtt tgcgagccgg gctggggagg
      841 tccagactgc ggcctgcagg agtgctcagc ctactgtggc agccatggca cctgcgcctc
      901 gaccctggga ccatgccgct gtgaacctgg attcttggga cgagcctgtg acctgcactt
      961 gtgggagaac cagggggctg gttggtggca cagtgtgagt gccggggacc ctgccttctc
     1021 tgcccgaatt ggggcagctg gtgcctttct gtccccacca gggctgctgg ctgtctttgg
     1081 aggccaggac cttaacaagg ccctgggtga ccttgtcctg tataacttct ccacgaacac
     1141 ctgggagtcc tgggacctga cgcctgctcc ggctgcccgt cattctcatg tggccgtggc
     1201 ctgggctggc ttgctggtgc tgatgggtgg tgagctggcc aatggcttgc taaccaatga
     1261 tgtgtgggcc tttagccccc tgggtggagg ccactgggag ctcctggcac cacctgcttc
     1321 cagttcttct gggcccccgg ggctggcagg ccatgcagct gctctggtgg atgacatctg
     1381 gctttatgtg tccggtggcc gcactcagca tgacctcttt tcttctggcc tcttccggtt
     1441 ccgcctggac cacaccagca ggggctattg ggagcaggtg atcccggcag gtgggcggcc
     1501 ccctgctgcc actgggcact ctatggtgtt ccatgcccca tctcgtactc tgctggtcca
     1561 tggtggacac cggccttcca ctgcccggtt ttctgtgcgg gtgaattcca ctgagctctt
     1621 ccacgtggaa cggcgtgtgt ggaccacgct gaagggccgg gatgggctcc agggtccacg
     1681 ggagagagcc ttccacactg ccagtgttct aggaaactac atggtggtct atgggggcaa
     1741 tgtgcatacg cattaccagg aggaaaagtg ttacgaagat ggcatcttct tctaccacct
     1801 tggctgccac cagtgggtgt caggggctga gcttgccccg ccaggaaccc ctgagggtcg
     1861 agcggcgcct cctagtggcc gttactcaca tgtggcagct gttcttggtg gcagtgtcct
     1921 gctggtggct gggggatata gtggccggcc tcgtggggac ttgatggcct acaaggtgcc
     1981 ccccttcgtg tttcaggcac ctgccctaga ttaccacctg gactattgtt ccatgtacac
     2041 agaccacagt gtctgctccc gagacccgga atgcagctgg tgtcagggcg cttgccaagc
     2101 tgcgccccct cctgggactc cctcaggagc ttgtccagcg gccagctgcc tgggcctagg
     2161 ccgcctgcta agtgactgcc aagcatgcct ggccttcagc agccccacag ctcctccccg
     2221 gggacctggc gccctaggtt ggtgtgtgca caatgagagc tgccttcctc ggcctgagca
     2281 ggcccgctgc cgaggggagc agatctcagg caccgtgggc tggtgggggc cagcacccgt
     2341 cttcgtcact tccctggagg cttgtgtcac ccagagcttc ctccctggcc tgcacctgct
     2401 caccttccag cagccaccca acgcctctca gcctgacaag gtctccattg tccgaagtac
     2461 aactatcacc ttgacaccca gcccggagac agatgtgtcc ctagtctacc gaggcttcat
     2521 ccaccccctg ctgccaggag gaccaggcgg ccctggggct gaagatgtgg ctgtgtgggc
     2581 tcgggcccag cgtctccatg tcctggcccg gatggccaga ggccctgaca ctgagaacat
     2641 ggaggaggtg ggacgctggg tggctcagca ggagaaggag acacggcgtc tgcagcgccc
     2701 tggctctgat cgcctcttcc cattgccggg gagaggcaac aagtacgcgg tggagatccg
     2761 aggccagctc aacggctcgg caggccctgg gcatagtgag ctcaccctcc tgtgggatcg
     2821 gactggagtg ccgggtggca gtgagatctc cttcttcttc ctggagccct accgctcttc
     2881 ggcctgcacc tcctactcct cctgtctggg ctgcctggct gatcagggct gtggctggtg
     2941 cctcaacagt gccacctgtc acctgcgtca aggcagggcc cactgtgagg atgatgggag
     3001 cggcgagtcc ctgctggtgc tggtgcctgc cctctgtcca ctctgtgagg agcatcgtga
     3061 ctgtcatgcc tgcacccagg accccttctg tgagtggcat cagagcacca accgcaaagg
     3121 agatgcagcg tgcagccggc gaggccgggg ccgaggcgct ctgaagaacc cagaggaatg
     3181 ccccccactc tgcagccaac ggctgacctg tgaggactgc ctggccaact ccagccagtg
     3241 tgcctggtgc cagtctactc acacctgctt cctgtttgct gcctacctgg cccggtaccc
     3301 acatgggggc tgccgtggct gggatgacag tgtgcactca gagccacggt gcaggagctg
     3361 cggtggcttt ttgacttgtc atgagtgtct gcagagccac gagtgtggtt ggtgtggcaa
     3421 cgaagacaac cccactctgg gacggtgcct acaaggggac ttctctggac ccctgggtgg
     3481 ggggaactgc tccctctggg tgggagaggg cctgggcctg cctgtggctc tccctgcccg
     3541 ctgggcttat gcccgctgtc cagatgtgga tgaatgtcgc ctgggcctgg cgaggtgtca
     3601 cccacgggca acctgcctga acacgcctct cagctatgag tgtcattgcc agcgtggcta
     3661 ccagggcgat ggcatcacac actgtaaccg cacgtgcctg gaagactgtg gccacggtgt
     3721 gtgtagcggt cctccggact tcacttgtgt gtgtgacctg ggctggacct cagacctgcc
     3781 cccacccacg cctgccccag gaccccctgc ccctcgttgc tctcgggact gtggctgcag
     3841 ctttcatagc cactgccgca ggcggggacc tggctactgt gacgagtgcc aggactggac
     3901 gtggggggaa cactgtgaac ggtgccggcc tggcagcttt ggcaatgcaa ccggctctgg
     3961 tggctgccga ccctgccagt gtaacgggca tggggaccca cgtcgtggcc actgtgacaa
     4021 cctcactggg ctctgcttct gccaggacca cactgagggt gcccactgtc agatttgctc
     4081 tccaggatac tatggggacc cccgagctgg tggctcctgc ttccgggagt gtgggggtcg
     4141 tgcgctcctc accaatgtgt cctcggtggc tctgggctcc cgccgctttg ggggacttct
     4201 gcctccaggt ggtggggcag caagagctgg gcccgggctg tcctactgcg tgtgggttgt
     4261 ctctgccaca gaagcgctgc agccctgtgt ccctggcacc ctctgccccc cgctcaccct
     4321 caccttctct ccagacagca gcacgccttg cacactgagc tacgtcctgg cctttgatgg
     4381 attcccacgc ttcctggaca ctggtgttgt tcagtcagac cggagcctca tagctgcctt
     4441 ctgcggccag cggcgtgaca gacctctcac tgtgcaggct ttatcagggt tacttgtgtt
     4501 gcactgggaa gccaacggct cctcctcctg gggattcaat gcttctgtgg gctctgcccg
     4561 gtgtggatca ggaggtcctg gcagttgccc tgtccctcag gaatgtgtgc cccaggatgg
     4621 ggctgcaggt gctggcctct gccgctgtcc tcagggctgg gctggccctc actgccgcat
     4681 ggctctgtgt cctgagaatt gcaacgccca caccggggca gggatttgta accagagcct
     4741 aggtgtgtgc atctgtgctg agggctttgg cggcccagac tgtgccacga agctggatgg
     4801 tggacagttg gtctgggaaa cgctcatgga cagccgcctc tcagctgata ctgctagtcg
     4861 cttccttcac cgcctgggac atactatggt agaggggcct gatgccactt tgtggatgtt
     4921 tgggggcctg ggtctgccac agggcctgct gggaaacctg tacaggtatt cagtcagtga
     4981 gcgcagatgg acacagatgt tggcaggagc tgaggatggg ggcccaggcc catcaccccg
     5041 ttccttccat gcagccgcct atgtgcctgc tggccgaggt gccatgtacc tgctgggggg
     5101 cctcacagct ggaggcgtca cccgtgactt ctgggtcctt aacctcacta ccctgcagtg
     5161 gagacaggag aagccccctc agaacatgga gttgccagcg gtcgctggtc acacccttac
     5221 tgcccgtcga ggcctatctc tgctcttggt gggtggctac tcccccgaaa atggcttcaa
     5281 ccagcagttg ttggagtacc agctagcaac cggcacctgg gtatctggag cccagagtgg
     5341 gacaccccca acaggtctct atggtcattc tgcagtctac cacgaggcca ccgactccct
     5401 ctatgtgttt gggggtttcc gcttccacgt ggagctggca gcaccttctc cggagctcta
     5461 ctccctgcac tgtcctgacc gtacctggag cctgctggca ccttctcagg gggcaaagcc
     5521 ccgaccccgg cttttccacg cttcagctct gctcggagac accatggtgg tccttggggg
     5581 gcgctcggac cctgatgaat tcagcagtga tgttctgctc taccaggtca actgcaacac
     5641 ctggctcctg cctgccctca cccgtccagc ctttgtgggg tccccgatgg aggagtctgt
     5701 ggcccatgca gtggcagcag ttggtagccg cctgtacatc tctggaggat ttgggggagt
     5761 agccttgggc cgcctgctgg cattgacctt gccccccgac ccctgccgtc tgctgccttc
     5821 acctgaagct tgtaatcagt ctggggcctg cacctggtgc catggtgctt gcctgtctgg
     5881 ggaccaggcc cacaggctgg gttgtggggt gcccccttgc tccccaatgc ctcgctcgcc
     5941 tgaggaatgt cggaggctgc ggacctgcag tgagtgcctg gcccgccatc ctcggaccct
     6001 gcagcctgga gatggagagg catccattcc ccgttgtaag tggtgtacca actgcccaga
     6061 gggtgcctgc attgggcgca atgggtcctg cacctcagag aatgactgcc gaatcaacca
     6121 gcgagaggtc ttctgggctg gcaactgctc agaggctgcg tgtggggctg ctgactgtga
     6181 gcagtgcacg cgggagggca agtgcatgtg gacgcggcag ttcaagagga ccggggagac
     6241 tcgccgaatc ctgtcagtgc agcccaccta tgactggact tgcttcagcc attccctgtt
     6301 gaatgtgtct cctatgccag tggagtcctc gcctccactg ccctgcccca cgccctgtca
     6361 cctcctgccc aactgcacat cgtgcctggc ctccaagggt gcagatgggg gctggcagca
     6421 ctgtgtgtgg agcagcagcc tccagcagtg tctgagcccc tcctatctgc ccttgcgatg
     6481 tatggctgga ggctgtgggc ggctgcttag agggccagag agctgctcgc tgggctgtgc
     6541 tcaggcgaca cagtgtgccc tgtgcctgcg acgaccccac tgtggctggt gtgcctgggg
     6601 aggccaggat gggggtggcc attgcatgga aggaggtctc agtggtcccc gagacgggct
     6661 aacgtgtgga cgtcccgggg cctcctgggc cttcctgtct tgcccccctg aggacgagtg
     6721 tgcaaatggg caccatgact gcaatgagac ccagaactgc catgaccagc cccacggcta
     6781 cgagtgcagc tgcaagacag gctacaccat ggacaatgtg actggcgtct gccgccctgt
     6841 gtgcgcccag ggctgtgtga acggatcctg tgtggagcct gatcactgcc gctgtcactt
     6901 tggctttgtt ggccgcaact gctccacaga gtgtcgctgc aaccgccaca gtgagtgtgc
     6961 cggcgttgga gcacaggacc actgcctgct gtgccggaac cacaccaagg gtagccactg
     7021 tgagcagtgt ctcccactgt tcgtgggttc ggctttgggc ggtgggacct gccgaccctg
     7081 ccatgccttc tgtcgcggga acagccatgt gtgcgtctcc aggaaagagc tagaaatggc
     7141 cagaaaggag ccagagaagt actcactgga tccagaggag attgaaacct gggtggcaga
     7201 aggtcccagt gaagatgaag ctgtgtgtgt gaactgtcag aacaacagct acggggacag
     7261 atgtgagagc tgcctccacg gctactttct cctggacggg aaatgcacca agtgccagtg
     7321 taatggccac gctgacacct gtaacgagca ggacgggaca ggatgcccat gtcagaacaa
     7381 cacagagaca ggcacctgcc agggcagctc tcccagtgac cgccgagact gctacaagta
     7441 ccagtgtgct aagtgccgcg agtcattcca cgggagcccg ctaggtggcc agcagtgtta
     7501 ccgactcatc tctgtggaac aagagtgctg cctggacccc acttcccaga ccaactgctt
     7561 ccatgagcca aagcgccggg ccctgggtcc tggccgcacc gtgctgtttg gcgtccaacc
     7621 caagttcacc aatgtggaca ttcgcctgac actagacgtg accttcggcg ctgtggacct
     7681 ctatgtctcc acctcctatg acacttttgt ggtccgagtg gcccctgaca caggcgtcca
     7741 cactgtgcac atccagccac ctccacctcc cccacctccc ccgcctcctg ctgatggtgt
     7801 acctcgggtg gctgcggacc taggaggatt ggggactgga agtgggtcag gatctccggt
     7861 ggagccacgg gtccgggagg tgtggccacg gggcctgatt acctacgtca cagtgacgga
     7921 accctcggca gtgctggtgg tccgcagtgt gagggacagg ctggtgatca cctatcccca
     7981 tgagcaccac gccctcaaat ccagccgctt ctacctgctg ctgctgggtg taggagaccc
     8041 caacgggcct ggtgccaacg gttcagcaga ctcacagggc ctgctcttct tccggcaaga
     8101 ccaggcccac attgacctgt tcgtcttctt ctctgtcttc ttttcctgct tcttcctctt
     8161 cctctcactc tgtgtgctcc tctggaaggc caagcaggct ctggaccagc gacaagagca
     8221 gcgccgccac ctgcaggaga tgaccaagat ggccagccgc ccctttgcca aggtcactgt
     8281 ctgcttccct ccagaccctg caggcccagc acctgcatgg aagccagctg ggctccctcc
     8341 tcctgccttc cgccgctctg agcctttcct agcacccctg ttgctgacag gagccggggg
     8401 tccctgggga cccatgggag gcgggtgctg cccaccagcc ctccctgcca ccacagctgg
     8461 cctacgagct ggacccatca cccttgagcc cacagaagat ggcatggctg gtgtggccac
     8521 tctgctgctc cagctgcctg ggggtcctca tgctcccaat ggggcctgcc ttgggtcagc
     8581 cctcgtcact ctgcgtcata ggctccatga gtactgtggg ggcagcgggg gtgctggagg
     8641 cagtggacac gggggtggtg gaggccggaa gggactgttg agtcaggaca atctcactag
     8701 catgtccctt tgacctgtca agggccctct tccacagcta ccatgttgcc tgatggggcc
     8761 actctgaact agggatcccc tggacagtct tcagaaacca ctggctccct tttcccaggg
     8821 tccccccctt tgttctgcat ttagcaacta tttattactt tgtgcccagt acagttgtgg
     8881 gtacaaagca aagcaggagg tgagaggagg ttccctcatc tggtggggat tgagttcttg
     8941 tggaaggaga catgtgccca catgcacaca cacatacaca cagtgtggca ctgaggtaaa
     9001 gttgatgaag catttggaga aactgggctg agaaaggtga cagacggagg gaagaccttt
     9061 gggaaacaaa ttcaagagtc gagggaactg taaggctgaa gggcccgagg ctggaagcgg
     9121 tttgtcttat cacgagaacc agagacctgg ccactgcaac ccagatcaag tgtcggctga
     9181 gctgggttct tagtctcagg gcagtgggca gcggttggac acttctgtgg tggtaaatga
     9241 ggggcttcag ctctggggag aagagtgggc tggtgggcac agaagacgct gcaggtggtt
     9301 aactgggcag gaggaaggtg agtggcagga tgtgacagtg gtgcagatgt tagagtaggg
     9361 aagaggacat atccagaacg gccactttat gtcctgggcc atgggtggac agtgaggttg
     9421 ttactaatct ggggcaggct ggggcaggct ggggcttgtg ggcttccagg tgtgactgcg
     9481 ggctcataca ggagggaatc tagtgccctg tggtgagttt agcactggca aaagcaggca
     9541 gctactgaga ggacaggaca gtgcagctgc ccgctgtagg agcagtgagc tgctgccggg
     9601 ctagaccagg gccaccagcg gaacctggag atgtaggctt atttaaaggc agcctcagag
     9661 gcaaattctt tgcctgagat gcctcacatc tcatcctggc aggtgtgggc tgggagaccc
     9721 tggaggggcc atcatttggc tttttcttga gtctctggga ctctgcacag aggccaccta
     9781 cacctggatc tcctttttgg acttaagagc tctctcctcc cccagtccta ggcagctgcc
     9841 tgagcttggg actcttgctt tcccaagact ccaacttcta gccgctgttt tggtcctggc
     9901 gtgggggttg gacccagcct ctttcccttg ctgcagttac cctgtctgat gtgatcctgc
     9961 taatgtgtgc atgtcagcat gggtcacatc tctatgatag gagggcaact gaggcaaaga
    10021 ataaagtgtg gagaagactt
//
