LOCUS       NM_027519               2953 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus mesenteric estrogen dependent adipogenesis (Medag),
            transcript variant 1, mRNA.
ACCESSION   NM_027519
VERSION     NM_027519.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2953)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 2953)
  AUTHORS   Tuck,E., Estabel,J., Oellrich,A., Maguire,A.K., Adissu,H.A.,
            Souter,L., Siragher,E., Lillistone,C., Green,A.L., Wardle-Jones,H.,
            Carragher,D.M., Karp,N.A., Smedley,D., Adams,N.C., Bussell,J.N.,
            Adams,D.J., Ramirez-Solis,R., Steel,K.P., Galli,A. and White,J.K.
  CONSRTM   Sanger Institute Mouse Genetics Project
  TITLE     A gene expression resource generated by genome-wide lacZ profiling
            in the mouse
  JOURNAL   Dis Model Mech 8 (11), 1467-1478 (2015)
   PUBMED   26398943
REFERENCE   5  (bases 1 to 2953)
  AUTHORS   Zhang,H., Chen,X. and Sairam,M.R.
  TITLE     Novel genes of visceral adiposity: identification of mouse and
            human mesenteric estrogen-dependent adipose (MEDA)-4 gene and its
            adipogenic function
  JOURNAL   Endocrinology 153 (6), 2665-2676 (2012)
   PUBMED   22510272
  REMARK    GeneRIF: study identifies Meda-4 as a novel adipogenic gene,
            capable of promoting differentiation of preadipocytes into
            adipocytes, increasing lipid content and glucose uptake in
            adipocytes; it might play an important role in adipose tissue
            expansion in normal and aberrant hormonal conditions and
            pathophysiological states
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK047657.1 and AK161526.1.
            
            On Oct 14, 2017 this sequence version replaced NM_027519.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK161526.1, AK160904.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2112              AK047657.1         1-2112
            2113-2953           AK161526.1         2065-2905
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 89.18 cM"
     gene            1..2953
                     /gene="Medag"
                     /gene_synonym="6330406I15Rik; Awms3; mAWMS3; MEDA-4;
                     Meda4"
                     /note="mesenteric estrogen dependent adipogenesis"
                     /db_xref="GeneID:70717"
                     /db_xref="MGI:MGI:1917967"
     exon            1..507
                     /gene="Medag"
                     /gene_synonym="6330406I15Rik; Awms3; mAWMS3; MEDA-4;
                     Meda4"
                     /inference="alignment:Splign:2.1.0"
     CDS             230..1141
                     /gene="Medag"
                     /gene_synonym="6330406I15Rik; Awms3; mAWMS3; MEDA-4;
                     Meda4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     activated in W/Wv mouse stomach 3; mesenteric
                     estrogen-dependent adipose 4; mesenteric
                     estrogen-dependent adipogenesis protein"
                     /codon_start=1
                     /product="mesenteric estrogen-dependent adipogenesis
                     protein isoform 1"
                     /protein_id="NP_081795.1"
                     /db_xref="CCDS:CCDS19884.1"
                     /db_xref="GeneID:70717"
                     /db_xref="MGI:MGI:1917967"
                     /translation="MATAACEPVARPSLTSISSGELRSLWTCDCELALLPLSQLLRLQ
                     PGAFQLRGEQLLVPGPGEPAAARGGFNVFGDGLVRLEGQLYRLSSYIKRYVELTNYCD
                     YKDYRETILSKPMVFFINVQTKKDISKERTYAFLVNTRHPKIRRQIEQGMDMVISSVI
                     GESYRLQFDFQEVVKNFFPPGTIVLNGENLSFTYEFKADALFDFFYWFGLSNSTVKVH
                     GKVLNLTSTNPEKKETIKLFLEKMSEPLIRRSSFSDRKFSVTSRGSIDDVFNCNLSPR
                     SSVTEPLLAEFSFPSLLECEETSSQLI"
     exon            508..617
                     /gene="Medag"
                     /gene_synonym="6330406I15Rik; Awms3; mAWMS3; MEDA-4;
                     Meda4"
                     /inference="alignment:Splign:2.1.0"
     exon            618..730
                     /gene="Medag"
                     /gene_synonym="6330406I15Rik; Awms3; mAWMS3; MEDA-4;
                     Meda4"
                     /inference="alignment:Splign:2.1.0"
     exon            731..1016
                     /gene="Medag"
                     /gene_synonym="6330406I15Rik; Awms3; mAWMS3; MEDA-4;
                     Meda4"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..2953
                     /gene="Medag"
                     /gene_synonym="6330406I15Rik; Awms3; mAWMS3; MEDA-4;
                     Meda4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2273..2278
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Medag"
                     /gene_synonym="6330406I15Rik; Awms3; mAWMS3; MEDA-4;
                     Meda4"
                     /note="hexamer: ATTAAA"
     polyA_site      2296
                     /gene="Medag"
                     /gene_synonym="6330406I15Rik; Awms3; mAWMS3; MEDA-4;
                     Meda4"
                     /note="major polyA site"
     regulatory      2628..2633
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Medag"
                     /gene_synonym="6330406I15Rik; Awms3; mAWMS3; MEDA-4;
                     Meda4"
                     /note="hexamer: AATAAA"
     polyA_site      2651
                     /gene="Medag"
                     /gene_synonym="6330406I15Rik; Awms3; mAWMS3; MEDA-4;
                     Meda4"
     regulatory      2912..2917
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Medag"
                     /gene_synonym="6330406I15Rik; Awms3; mAWMS3; MEDA-4;
                     Meda4"
                     /note="hexamer: ATTAAA"
     polyA_site      2929
                     /gene="Medag"
                     /gene_synonym="6330406I15Rik; Awms3; mAWMS3; MEDA-4;
                     Meda4"
ORIGIN      
        1 gaggggcgtg cctggtgcct gataaaaggg gctgcgcagc gcggggaccg ctttctcagt
       61 gtcctgcctg cagcgctcca caagggtggg caaccttcaa ggtgacaaga gggctcctga
      121 gctacttact acttgcacgc agcccaaggt agctcgggac agaccacaga tttactcctc
      181 ccacttagcg accctcaggg taggaagggg acagagccac agcctgagta tggccactgc
      241 agcgtgcgag cctgtggcca gacccagcct gacctccatt tcatccgggg agctgcgcag
      301 tctgtggacg tgcgactgcg agctggccct gctgcccctg tcgcagctgc tccgcctaca
      361 gcctggtgcc ttccaactgc gcggtgagca gctcctggtg cccgggcccg gggaacccgc
      421 ggccgcacgt gggggcttca acgtcttcgg ggacggcctt gtgcgcctag aagggcagct
      481 ctatcgcctc agcagctaca tcaagagata tgtggaactg accaactact gtgattataa
      541 agactacagg gaaacgatac tgagcaaacc tatggtattc tttattaacg tgcagaccaa
      601 gaaggacatt tcaaaagaaa ggacctatgc gtttcttgtg aacacaaggc accccaagat
      661 aagaagacag atagagcaag ggatggacat ggtcatttcc tcagtgattg gagaaagcta
      721 tagacttcag tttgatttcc aggaagtagt gaagaatttc ttccctccag gaacgattgt
      781 gctgaatgga gagaatttga gcttcaccta cgaattcaag gcagatgccc tgtttgattt
      841 cttctactgg tttgggctca gcaattccac cgtaaaagtg cacggaaagg ttctgaactt
      901 gacaagtacg aacccagaaa agaaagaaac aatcaagtta tttctggaaa aaatgagtga
      961 acctctaata cgaaggagta gtttctctga tcggaaattt agcgtcacat ctagaggttc
     1021 aatagatgac gttttcaact gcaatctgtc acccagatca tcggttacag aaccactttt
     1081 ggcagaattc tcatttccaa gtcttctgga atgtgaagag acatccagcc aacttatctg
     1141 attggtctga acattcaagc tgtcgccctt tgggggtctg tggcacacag tgggttaaag
     1201 gggtaaaaga aggcagggag tggacatttc tatccctttg ccagcctctg gccctccaag
     1261 gttctataga ggaccccctt tcccatccct aggcactggc aggcccagtg gcttccgtca
     1321 aggttatgat tatgggatgg aaggattttg tctctgtggc atagggtgtc attgacattg
     1381 aacacttctc tgaggaattt tcaggggtaa ccaagcaccc atgaactttg cttagtaccc
     1441 tggcactctg aagaccctcc tggcaacctc tgtgggaggc atctgactgg gatgctaacc
     1501 tgtgatgtat cctggatcac agaatggccc tctgcagcca gcagccccag caagccttgg
     1561 ggctaaactc ttctcttgca aatatgtcag gtaaagccag tactgatatt tccctgggaa
     1621 acaaatctga tgtccaacct gccactggaa tgaacattca agatactggc tgctgaagga
     1681 tctctgcagg ccaccaggat gagccctccc tgaaatgtaa agccaggtgg ggtgccaatc
     1741 aaccagtgac aagggagggt cctcctccct acaggtacac cgtagtagtt ggtgtttgaa
     1801 atacacttta acaatcttaa tatgagaaaa agagcccagg gaaactaagc cattaccatg
     1861 gtacactgga catcagggcg ctttctgccc cacagctcca gtgggattca ctgaaagggg
     1921 aaaaaaatga tccaatccac atataaaagt ttgcgtgagt ggccgggcct ggtgcctgta
     1981 attctagcac tcgggaggtg gaggaagtag aatgaggagt tcaagaccac ccttggctac
     2041 atcaggagtt caaggccaac ttgctctgtt actacaggag actgtctaat aaaaacaaac
     2101 ctgcatcatt gcacatgtaa atttgcacct atactcataa gaaggcagat ttatgggccc
     2161 ctagtggcat cttttctgtc cttctaaaaa catattccca cactacatat tttgatagca
     2221 aggttgatgg ccagccattt gtatctgaga tgctacctag tacatatgca caattaaaag
     2281 ttttcttagc gttcaacttg taatggattt aatttgtggc taaaaaaagg tacagggtag
     2341 ccccttgagg tctgtgggtg gtgtggttgc tctgtggaac actaagaatg ggagggttgt
     2401 ggctttaaca gctgctacct caagaaggga cttagagaga gaagccatag agtatttgga
     2461 gaaagaaaat gaaggttgag aaggagaaaa gaaagtgtca cttcgtcata ggaggataaa
     2521 tatgttatta aattcagtaa aaaggaaaat aataaattct aaccggttct ccaatcagca
     2581 gaaataaacc ttgggtacac agcaagaatt aaactcttcc caaaggaaat aaaaactatt
     2641 cataacattg acaattttct tttgaaactg aactgagaaa atagtcagta atcaccccag
     2701 gaaatggact gaagatctgg gaagttaatt tcatcattaa gatgtcttag ttgggctgga
     2761 gagatggctc agcagttatt agcattgact gctcttccag aggtcctgag tccaattctc
     2821 agcaaccaca tggtggctca caatcatctg taatgggaat ccgatgccct cttctggtgt
     2881 gtctgaagac agctacagtg tattcacata cattaaataa ataaataaat cttaaaaaaa
     2941 aaaaagtcct act
//
