LOCUS       NM_020000               1887 bp    mRNA    linear   ROD 18-JUN-2024
DEFINITION  Mus musculus mediator complex subunit 8 (Med8), transcript variant
            1, mRNA.
ACCESSION   NM_020000
VERSION     NM_020000.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Kolonay,D.W., Sattler,K.M., Strawser,C., Rafael-Fortney,J.,
            Mihaylova,M.M., Miller,K.E., Lepper,C. and Baskin,K.K.
  TITLE     Temporal regulation of the Mediator complex during muscle
            proliferation, differentiation, regeneration, aging, and disease
  JOURNAL   Front Cell Dev Biol 12, 1331563 (2024)
   PUBMED   38690566
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Tan,C., Zhu,S., Chen,Z., Liu,C., Li,Y.E., Zhu,M., Zhang,Z.,
            Zhang,Z., Zhang,L., Gu,Y., Liang,Z., Boyer,T.G., Ouyang,K.,
            Evans,S.M. and Fang,X.
  TITLE     Mediator complex proximal Tail subunit MED30 is critical for
            Mediator core stability and cardiomyocyte transcriptional network
  JOURNAL   PLoS Genet 17 (9), e1009785 (2021)
   PUBMED   34506481
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1887)
  AUTHORS   Tsai,K.L., Tomomori-Sato,C., Sato,S., Conaway,R.C., Conaway,J.W.
            and Asturias,F.J.
  TITLE     Subunit architecture and functional modular rearrangements of the
            transcriptional mediator complex
  JOURNAL   Cell 157 (6), 1430-1444 (2014)
   PUBMED   24882805
  REMARK    Erratum:[Cell. 2014 Jul 17;158(2):463]
REFERENCE   4  (bases 1 to 1887)
  AUTHORS   Hammock,E.A., Eagleson,K.L., Barlow,S., Earls,L.R., Miller,D.M. 3rd
            and Levitt,P.
  TITLE     Homologs of genes expressed in Caenorhabditis elegans GABAergic
            neurons are also found in the developing mouse forebrain
  JOURNAL   Neural Dev 5, 32 (2010)
   PUBMED   21122108
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1887)
  AUTHORS   Krebs,A.R., Demmers,J., Karmodiya,K., Chang,N.C., Chang,A.C. and
            Tora,L.
  TITLE     ATAC and Mediator coactivators form a stable complex and regulate a
            set of non-coding RNA genes
  JOURNAL   EMBO Rep 11 (7), 541-547 (2010)
   PUBMED   20508642
REFERENCE   6  (bases 1 to 1887)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
REFERENCE   7  (bases 1 to 1887)
  AUTHORS   Frankel,W.N., Yang,Y., Mahaffey,C.L., Beyer,B.J. and O'Brien,T.P.
  TITLE     Szt2, a novel gene for seizure threshold in mice
  JOURNAL   Genes Brain Behav 8 (5), 568-576 (2009)
   PUBMED   19624305
REFERENCE   8  (bases 1 to 1887)
  AUTHORS   Tomomori-Sato,C., Sato,S., Parmely,T.J., Banks,C.A., Sorokina,I.,
            Florens,L., Zybailov,B., Washburn,M.P., Brower,C.S., Conaway,R.C.
            and Conaway,J.W.
  TITLE     A mammalian mediator subunit that shares properties with
            Saccharomyces cerevisiae mediator subunit Cse2
  JOURNAL   J Biol Chem 279 (7), 5846-5851 (2004)
   PUBMED   14638676
REFERENCE   9  (bases 1 to 1887)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK011080.1, AK008759.1, AK148025.1 and BY429746.1.
            
            On Mar 19, 2014 this sequence version replaced NM_020000.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660813.115113.1,
                                           SRR1660815.159758.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                AK011080.1         2-25
            25-1172             AK008759.1         4-1151
            1173-1842           AK148025.1         1154-1823
            1843-1887           BY429746.1         368-412
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 54.61 cM"
     gene            1..1887
                     /gene="Med8"
                     /gene_synonym="2210021A15Rik; ARC32"
                     /note="mediator complex subunit 8"
                     /db_xref="GeneID:80509"
                     /db_xref="MGI:MGI:1915269"
     exon            1..50
                     /gene="Med8"
                     /gene_synonym="2210021A15Rik; ARC32"
                     /inference="alignment:Splign:2.1.0"
     CDS             45..851
                     /gene="Med8"
                     /gene_synonym="2210021A15Rik; ARC32"
                     /note="isoform 1 is encoded by transcript variant 1;
                     activator-recruited cofactor 32 kDa component; mediator of
                     RNA polymerase II transcription, subunit 8 homolog"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 8 isoform 1"
                     /protein_id="NP_064384.2"
                     /db_xref="CCDS:CCDS38854.1"
                     /db_xref="GeneID:80509"
                     /db_xref="MGI:MGI:1915269"
                     /translation="MQREEKQLEASLDALLNQVADLKNSLGSFIYKLENEYDRLTWPS
                     VLDSFALLSGQLNTLNKVLKHEKTPLFRNQVIIPLVLSPDRDEDLMRQTEGRVPVFSH
                     EVVPDHLRTKPDPEVEEQEKQLTTDAARIGADAAQKQIQSLNKMCSNLLEKISKEERE
                     SESGGLRPNKQTFNPGDTNALVAAVAFGKGLSNWRPSGSSGPGQPGQPGAGTILAGAS
                     GLPQVQMPGAPNQQQPMLSGVQMAQAGQPGKMPSGIKTNIKSASMHPYQR"
     exon            51..169
                     /gene="Med8"
                     /gene_synonym="2210021A15Rik; ARC32"
                     /inference="alignment:Splign:2.1.0"
     exon            170..314
                     /gene="Med8"
                     /gene_synonym="2210021A15Rik; ARC32"
                     /inference="alignment:Splign:2.1.0"
     exon            315..455
                     /gene="Med8"
                     /gene_synonym="2210021A15Rik; ARC32"
                     /inference="alignment:Splign:2.1.0"
     exon            456..537
                     /gene="Med8"
                     /gene_synonym="2210021A15Rik; ARC32"
                     /inference="alignment:Splign:2.1.0"
     exon            538..786
                     /gene="Med8"
                     /gene_synonym="2210021A15Rik; ARC32"
                     /inference="alignment:Splign:2.1.0"
     exon            787..1887
                     /gene="Med8"
                     /gene_synonym="2210021A15Rik; ARC32"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1148..1153
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Med8"
                     /gene_synonym="2210021A15Rik; ARC32"
                     /note="hexamer: AATAAA"
     polyA_site      1174
                     /gene="Med8"
                     /gene_synonym="2210021A15Rik; ARC32"
                     /note="major polyA site"
     regulatory      1825..1830
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Med8"
                     /gene_synonym="2210021A15Rik; ARC32"
                     /note="hexamer: AATAAA"
     polyA_site      1845
                     /gene="Med8"
                     /gene_synonym="2210021A15Rik; ARC32"
ORIGIN      
        1 gagaaaccgg aaagggaagt ggttgcgccc gccgggccgc ggcaatgcag agggaggaga
       61 agcagcttga ggcatcatta gatgccctgc tgaatcaagt ggccgatctg aagaattcac
      121 tggggagttt catttacaag ttggaaaacg agtatgaccg gctgacctgg ccctctgtcc
      181 tggacagctt tgcattgctt tctggacagt tgaacactct gaacaaggtc ttaaagcatg
      241 agaagacgcc actgttccga aaccaggtca tcatccctct ggtgctgtcc ccagaccgag
      301 acgaagacct catgcgtcaa actgaaggac gagtgcctgt tttcagccat gaggtcgttc
      361 ctgaccactt gagaaccaag cctgaccctg aagttgaaga gcaggagaag cagctgacaa
      421 cagatgctgc cagaattggt gctgatgcag ctcagaagca gatccagagc ttgaataaaa
      481 tgtgctcaaa ccttttagag aaaatcagca aagaagaacg agaatcagag agcggaggtc
      541 tccggccaaa caagcagact ttcaaccctg gagacaccaa tgccttagta gcagccgttg
      601 cctttgggaa ggggctatct aattggcgac cttcaggtag cagtggtcct gggcaacccg
      661 gtcagccagg agctggtaca atccttgcag gggcctcagg attaccgcag gttcagatgc
      721 caggtgctcc aaaccagcag cagccaatgc tcagtggagt tcagatggct caggcaggtc
      781 aaccagggaa aatgccaagt ggaataaaaa ccaacatcaa gtcagcttca atgcatccgt
      841 accagcggtg agtgtggacg gcagcctcca ctctcaggtg atctgtgctg agttgagcaa
      901 tgacatgatt ttttgctcaa ggcttattta gatggggaca ataaaaagaa cacaggcttt
      961 cagcaagaga cagatctcag ctccactgtg aatagccatt cgaacttggg aaagtgacct
     1021 aatatttctg agcctgtgtt ctcatttgta gatggtgata atacctacct cgtagggttg
     1081 ttgtgaggat taaaatgaga aaatgaatgt aaaacactta gcatggtgta tgcaattctg
     1141 ggtcttgaat aaatgatagt ttcctacctt tcaaccctgc cattccttgt cctatatgca
     1201 gaacttattg tgatttggtt catctccatg acaccagtga gccaatgggc ccaggatcaa
     1261 gttatccatg gtggcctgta tgtgttgggg gcatactgtc tctgcaggcc ctcctgccca
     1321 agtacggcct gctggcccta aaagctacag gtgaggaggt ttctggagga cactggccac
     1381 ttccaggtgt gatgagacca gctcagcatt tccctgggaa ataggtaaac atgcccatct
     1441 tttagcctcc tgtgctgcta aagatgccca gggtccagca ggtggaagag ctcttaagag
     1501 ggactctggc aggcaacagc acggagaata ctgtcttcct ccgtggaggg cagttgtgtt
     1561 ttatagtgcc gtgggttggg gttcatttgt ctgcacagtg acgactccct gtctctgtcc
     1621 tcttccatct cactgaacgt gtggctcaga taagatctgg attgaaagtg ggaagggcag
     1681 cagctccatt catattgcct gcttttgtag agttgacgaa cagtggagtt ttggacagcc
     1741 aggcctgttg cctttctcag cgttcttttc tgggcaaggg cagagattga gtcagcagaa
     1801 ttgggccaaa gggtttcttt gtttaataaa gatatttaaa ttatgttccc tggctcaggg
     1861 ttgtctgact aaagtttatg agctatc
//
