LOCUS       NM_001347384            1061 bp    mRNA    linear   ROD 12-FEB-2025
DEFINITION  Mus musculus mediator complex subunit 6 (Med6), transcript variant
            1, mRNA.
ACCESSION   NM_001347384 XM_006516211
VERSION     NM_001347384.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Kolonay,D.W., Sattler,K.M., Strawser,C., Rafael-Fortney,J.,
            Mihaylova,M.M., Miller,K.E., Lepper,C. and Baskin,K.K.
  TITLE     Temporal regulation of the Mediator complex during muscle
            proliferation, differentiation, regeneration, aging, and disease
  JOURNAL   Front Cell Dev Biol 12, 1331563 (2024)
   PUBMED   38690566
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Tan,C., Zhu,S., Chen,Z., Liu,C., Li,Y.E., Zhu,M., Zhang,Z.,
            Zhang,Z., Zhang,L., Gu,Y., Liang,Z., Boyer,T.G., Ouyang,K.,
            Evans,S.M. and Fang,X.
  TITLE     Mediator complex proximal Tail subunit MED30 is critical for
            Mediator core stability and cardiomyocyte transcriptional network
  JOURNAL   PLoS Genet 17 (9), e1009785 (2021)
   PUBMED   34506481
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1061)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1061)
  AUTHORS   Aranda-Orgilles,B., Saldana-Meyer,R., Wang,E., Trompouki,E.,
            Fassl,A., Lau,S., Mullenders,J., Rocha,P.P., Raviram,R.,
            Guillamot,M., Sanchez-Diaz,M., Wang,K., Kayembe,C., Zhang,N.,
            Amoasii,L., Choudhuri,A., Skok,J.A., Schober,M., Reinberg,D.,
            Sicinski,P., Schrewe,H., Tsirigos,A., Zon,L.I. and Aifantis,I.
  TITLE     MED12 Regulates HSC-Specific Enhancers Independently of Mediator
            Kinase Activity to Control Hematopoiesis
  JOURNAL   Cell Stem Cell 19 (6), 784-799 (2016)
   PUBMED   27570068
REFERENCE   5  (bases 1 to 1061)
  AUTHORS   Thompson,C.L., Ng,L., Menon,V., Martinez,S., Lee,C.K.,
            Glattfelder,K., Sunkin,S.M., Henry,A., Lau,C., Dang,C.,
            Garcia-Lopez,R., Martinez-Ferre,A., Pombero,A., Rubenstein,J.L.R.,
            Wakeman,W.B., Hohmann,J., Dee,N., Sodt,A.J., Young,R., Smith,K.,
            Nguyen,T.N., Kidney,J., Kuan,L., Jeromin,A., Kaykas,A., Miller,J.,
            Page,D., Orta,G., Bernard,A., Riley,Z., Smith,S., Wohnoutka,P.,
            Hawrylycz,M.J., Puelles,L. and Jones,A.R.
  TITLE     A high-resolution spatiotemporal atlas of gene expression of the
            developing mouse brain
  JOURNAL   Neuron 83 (2), 309-323 (2014)
   PUBMED   24952961
REFERENCE   6  (bases 1 to 1061)
  AUTHORS   Malik,S., Guermah,M., Yuan,C.X., Wu,W., Yamamura,S. and Roeder,R.G.
  TITLE     Structural and functional organization of TRAP220, the
            TRAP/mediator subunit that is targeted by nuclear receptors
  JOURNAL   Mol Cell Biol 24 (18), 8244-8254 (2004)
   PUBMED   15340084
  REMARK    Erratum:[Mol Cell Biol. 2022 Nov 17;42(11):e0035922. doi:
            10.1128/mcb.00359-22.. PMID: 36255214]
REFERENCE   7  (bases 1 to 1061)
  AUTHORS   Tomomori-Sato,C., Sato,S., Parmely,T.J., Banks,C.A., Sorokina,I.,
            Florens,L., Zybailov,B., Washburn,M.P., Brower,C.S., Conaway,R.C.
            and Conaway,J.W.
  TITLE     A mammalian mediator subunit that shares properties with
            Saccharomyces cerevisiae mediator subunit Cse2
  JOURNAL   J Biol Chem 279 (7), 5846-5851 (2004)
   PUBMED   14638676
REFERENCE   8  (bases 1 to 1061)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 1061)
  AUTHORS   Suzuki,H., Fukunishi,Y., Kagawa,I., Saito,R., Oda,H., Endo,T.,
            Kondo,S., Bono,H., Okazaki,Y. and Hayashizaki,Y.
  TITLE     Protein-protein interaction panel using mouse full-length cDNAs
  JOURNAL   Genome Res 11 (10), 1758-1765 (2001)
   PUBMED   11591653
REFERENCE   10 (bases 1 to 1061)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154908.4, CK620521.1 and DV651747.1.
            
            On Nov 24, 2016 this sequence version replaced XM_006516211.3.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC013096.1, AK005232.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                AC154908.4         148745-148760       c
            17-621              CK620521.1         1-605
            622-1054            DV651747.1         58-490
            1055-1061           AC154908.4         127309-127315       c
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 37.8 cM"
     gene            1..1061
                     /gene="Med6"
                     /gene_synonym="1500012F11Rik"
                     /note="mediator complex subunit 6"
                     /db_xref="GeneID:69792"
                     /db_xref="MGI:MGI:1917042"
     exon            1..69
                     /gene="Med6"
                     /gene_synonym="1500012F11Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             48..788
                     /gene="Med6"
                     /gene_synonym="1500012F11Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     mediator of RNA polymerase II transcription subunit 6;
                     mediator of RNA polymerase II transcription, subunit 6
                     homolog"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 6 isoform 1"
                     /protein_id="NP_001334313.1"
                     /db_xref="CCDS:CCDS83983.1"
                     /db_xref="GeneID:69792"
                     /db_xref="MGI:MGI:1917042"
                     /translation="MAAVDIRDNLLGISWVDSSWIPILNSGSVLDYFSERSNPFYDRT
                     CNNEVVKMQRLTLEHLNQMVGIEYILLHAQEPILFIIRKQQRQSPAQVIPLADYYIIA
                     GVIYQAPDLGSVINSRVLTAVHGIQSAFDEAMSYCRYHPSKGYWWHFKDHEEQEKVKP
                     KAKRKEEPSSIFQRQRVDALLIDLRQKFPPRFVQQKSGEKPVPVDQAKKEAEPLPETV
                     KSEEKESTKNIQQTVSTKGPPEKRMRLQ"
     exon            70..229
                     /gene="Med6"
                     /gene_synonym="1500012F11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            230..321
                     /gene="Med6"
                     /gene_synonym="1500012F11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            322..404
                     /gene="Med6"
                     /gene_synonym="1500012F11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            405..513
                     /gene="Med6"
                     /gene_synonym="1500012F11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            514..629
                     /gene="Med6"
                     /gene_synonym="1500012F11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            630..657
                     /gene="Med6"
                     /gene_synonym="1500012F11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            658..1061
                     /gene="Med6"
                     /gene_synonym="1500012F11Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1032..1037
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Med6"
                     /gene_synonym="1500012F11Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1054
                     /gene="Med6"
                     /gene_synonym="1500012F11Rik"
                     /note="major polyA site"
ORIGIN      
        1 gcctttactg tgcgccggaa ggaggctctt atagcgcttt tggaattatg gcggcggtgg
       61 atatccgaga taacctgctg gggatctcct gggttgacag ctcttggatc cccatcttga
      121 acagtggcag tgtcctggat tacttttcag aaagaagcaa tcctttctat gacaggacgt
      181 gtaataatga ggtggtcaaa atgcagaggc tgacattaga gcacttgaat cagatggttg
      241 gaatcgaata catcctttta catgctcaag agcccattct tttcatcatt cgaaagcaac
      301 agcggcagtc ccctgctcaa gttattccac tggctgatta ctatatcatc gctggagtca
      361 tctatcaggc gccagacttg ggatcagtta taaactccag agtgcttact gcagtgcatg
      421 gaattcagtc agcttttgat gaagctatgt catactgtcg ataccatcct tccaaaggat
      481 attggtggca ctttaaagat cacgaagaac aagagaaagt caaacctaaa gccaaaagga
      541 aagaagaacc aagctctatt tttcaaagac agcgtgtgga tgctttactc atagacctta
      601 gacagaaatt tccacccaga tttgttcagc aaaagtctgg agaaaagcct gtcccagtgg
      661 atcaggcaaa aaaagaggca gagcctttgc cagaaactgt aaaatctgag gagaaggaga
      721 gcacaaagaa cattcagcag actgtgagca ctaaaggtcc ccctgagaaa cgaatgagac
      781 tccagtgaat gttggacgag gaagaagctg gccagctcct tgtcagtcgc tatacctcag
      841 caccgtggga ggctcttgag cctggaaaca ctatcgcagt ttgtacggaa tgcacttgtt
      901 ctttacaaaa ggacgttctc cctaccatgt tttctatgtg aatatttctt aaatatcttt
      961 tgtaatcttt cctctactgg acttgggcag tgcacctaac tcacagatat gccagtatat
     1021 atctttaaaa caataaagtt atttgtatat attactcatc a
//
