LOCUS       NM_026119               1304 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus mediator complex subunit 4 (Med4), mRNA.
ACCESSION   NM_026119
VERSION     NM_026119.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Kolonay,D.W., Sattler,K.M., Strawser,C., Rafael-Fortney,J.,
            Mihaylova,M.M., Miller,K.E., Lepper,C. and Baskin,K.K.
  TITLE     Temporal regulation of the Mediator complex during muscle
            proliferation, differentiation, regeneration, aging, and disease
  JOURNAL   Front Cell Dev Biol 12, 1331563 (2024)
   PUBMED   38690566
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Tan,C., Zhu,S., Chen,Z., Liu,C., Li,Y.E., Zhu,M., Zhang,Z.,
            Zhang,Z., Zhang,L., Gu,Y., Liang,Z., Boyer,T.G., Ouyang,K.,
            Evans,S.M. and Fang,X.
  TITLE     Mediator complex proximal Tail subunit MED30 is critical for
            Mediator core stability and cardiomyocyte transcriptional network
  JOURNAL   PLoS Genet 17 (9), e1009785 (2021)
   PUBMED   34506481
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1304)
  AUTHORS   Sun,F., Sun,T., Kronenberg,M., Tan,X., Huang,C. and Carey,M.F.
  TITLE     The Pol II preinitiation complex (PIC) influences Mediator binding
            but not promoter-enhancer looping
  JOURNAL   Genes Dev 35 (15-16), 1175-1189 (2021)
   PUBMED   34301767
  REMARK    GeneRIF: The Pol II preinitiation complex (PIC) influences Mediator
            binding but not promoter-enhancer looping.
REFERENCE   4  (bases 1 to 1304)
  AUTHORS   Aranda-Orgilles,B., Saldana-Meyer,R., Wang,E., Trompouki,E.,
            Fassl,A., Lau,S., Mullenders,J., Rocha,P.P., Raviram,R.,
            Guillamot,M., Sanchez-Diaz,M., Wang,K., Kayembe,C., Zhang,N.,
            Amoasii,L., Choudhuri,A., Skok,J.A., Schober,M., Reinberg,D.,
            Sicinski,P., Schrewe,H., Tsirigos,A., Zon,L.I. and Aifantis,I.
  TITLE     MED12 Regulates HSC-Specific Enhancers Independently of Mediator
            Kinase Activity to Control Hematopoiesis
  JOURNAL   Cell Stem Cell 19 (6), 784-799 (2016)
   PUBMED   27570068
REFERENCE   5  (bases 1 to 1304)
  AUTHORS   Krebs,A.R., Demmers,J., Karmodiya,K., Chang,N.C., Chang,A.C. and
            Tora,L.
  TITLE     ATAC and Mediator coactivators form a stable complex and regulate a
            set of non-coding RNA genes
  JOURNAL   EMBO Rep 11 (7), 541-547 (2010)
   PUBMED   20508642
REFERENCE   6  (bases 1 to 1304)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
REFERENCE   7  (bases 1 to 1304)
  AUTHORS   Malik,S., Guermah,M., Yuan,C.X., Wu,W., Yamamura,S. and Roeder,R.G.
  TITLE     Structural and functional organization of TRAP220, the
            TRAP/mediator subunit that is targeted by nuclear receptors
  JOURNAL   Mol Cell Biol 24 (18), 8244-8254 (2004)
   PUBMED   15340084
  REMARK    Erratum:[Mol Cell Biol. 2022 Nov 17;42(11):e0035922. doi:
            10.1128/mcb.00359-22.. PMID: 36255214]
REFERENCE   8  (bases 1 to 1304)
  AUTHORS   Tomomori-Sato,C., Sato,S., Parmely,T.J., Banks,C.A., Sorokina,I.,
            Florens,L., Zybailov,B., Washburn,M.P., Brower,C.S., Conaway,R.C.
            and Conaway,J.W.
  TITLE     A mammalian mediator subunit that shares properties with
            Saccharomyces cerevisiae mediator subunit Cse2
  JOURNAL   J Biol Chem 279 (7), 5846-5851 (2004)
   PUBMED   14638676
REFERENCE   9  (bases 1 to 1304)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 1304)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC113198.15.
            
            On Nov 17, 2006 this sequence version replaced NM_026119.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC024950.1, CX216857.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-137               AC113198.15        61915-62051         c
            138-204             AC113198.15        60879-60945         c
            205-375             AC113198.15        58125-58295         c
            376-433             AC113198.15        56753-56810         c
            434-520             AC113198.15        55378-55464         c
            521-652             AC113198.15        54762-54893         c
            653-1304            AC113198.15        53555-54206         c
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 39.05 cM"
     gene            1..1304
                     /gene="Med4"
                     /gene_synonym="2410046H15Rik; DRIP36; HSPC126; TRAP36;
                     Vdirp; Vdrip"
                     /note="mediator complex subunit 4"
                     /db_xref="GeneID:67381"
                     /db_xref="MGI:MGI:1914631"
     exon            1..137
                     /gene="Med4"
                     /gene_synonym="2410046H15Rik; DRIP36; HSPC126; TRAP36;
                     Vdirp; Vdrip"
                     /inference="alignment:Splign:2.1.0"
     CDS             13..825
                     /gene="Med4"
                     /gene_synonym="2410046H15Rik; DRIP36; HSPC126; TRAP36;
                     Vdirp; Vdrip"
                     /note="vitamin D receptor interacting protein; p36
                     TRAP/SMCC/PC2 subunit; mediator of RNA polymerase II
                     transcription, subunit 4 homolog"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 4"
                     /protein_id="NP_080395.1"
                     /db_xref="CCDS:CCDS49538.1"
                     /db_xref="GeneID:67381"
                     /db_xref="MGI:MGI:1914631"
                     /translation="MAASSSGEKEKERMGGVSGMAGLGSTRERLLSALEDLEVLSREL
                     IEMLAISRNQKLLQLEEENQVLELLIHRDGDFQELMKLALNQGKVHHEMQALEKEVEK
                     RDSDIQQLQKQLKEAEQILATAVYQAKEKLKSIEKARKGAISSEEIIKYAHRISASNA
                     VCAPLTWVPGDPRRPYPTDLEMRSGLLGQMNNPSTSGVNGHLPGDALAAGRLPDVLAP
                     QYPWQSNDMSVNMLPPNHSSDFLLEPPGHNKENEDDVEVMSTDSSSSSSDSD"
     exon            138..204
                     /gene="Med4"
                     /gene_synonym="2410046H15Rik; DRIP36; HSPC126; TRAP36;
                     Vdirp; Vdrip"
                     /inference="alignment:Splign:2.1.0"
     exon            205..375
                     /gene="Med4"
                     /gene_synonym="2410046H15Rik; DRIP36; HSPC126; TRAP36;
                     Vdirp; Vdrip"
                     /inference="alignment:Splign:2.1.0"
     exon            376..433
                     /gene="Med4"
                     /gene_synonym="2410046H15Rik; DRIP36; HSPC126; TRAP36;
                     Vdirp; Vdrip"
                     /inference="alignment:Splign:2.1.0"
     exon            434..520
                     /gene="Med4"
                     /gene_synonym="2410046H15Rik; DRIP36; HSPC126; TRAP36;
                     Vdirp; Vdrip"
                     /inference="alignment:Splign:2.1.0"
     exon            521..652
                     /gene="Med4"
                     /gene_synonym="2410046H15Rik; DRIP36; HSPC126; TRAP36;
                     Vdirp; Vdrip"
                     /inference="alignment:Splign:2.1.0"
     exon            653..1304
                     /gene="Med4"
                     /gene_synonym="2410046H15Rik; DRIP36; HSPC126; TRAP36;
                     Vdirp; Vdrip"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtcagcgggg cgatggcggc ttcttcaagt ggtgagaagg aaaaggagcg gatgggaggc
       61 gtttcgggaa tggccggcct tggcagtacg cgagagcggc ttctgtctgc gctggaagat
      121 ctggaggtct tgtcgaggga actcatagaa atgcttgcaa tttcaagaaa tcagaagcta
      181 ttacagctgg aagaggaaaa ccaggtcttg gagttgctaa ttcaccgaga tggggacttc
      241 caagaactaa tgaaattagc acttaatcag ggaaaagttc accacgaaat gcaggcttta
      301 gagaaagaag tagagaagag agacagtgat attcagcagc tgcagaaaca gctgaaagag
      361 gccgagcaga tcctggcaac agctgtttac caggcaaagg aaaaactcaa gtcaatagag
      421 aaagcaagaa aaggtgctat ctcctctgaa gaaatcatta aatatgcgca caggattagt
      481 gcaagcaatg ctgtctgtgc tccactgact tgggttccag gagaccctcg gaggccatac
      541 ccaaccgatt tagagatgag aagtggattg ttgggtcaga tgaacaatcc ttccaccagt
      601 ggtgtgaatg gtcacctccc gggagatgcc cttgcagcag gtagactgcc agatgtcctt
      661 gctccgcagt acccatggca gtcaaatgac atgtctgtga acatgctgcc accaaatcac
      721 agcagtgact ttctgttgga gcctcctggg cacaacaaag aaaatgaaga tgatgttgag
      781 gttatgtcaa cagactcttc gagcagcagt agcgactctg actgagaaag gtgtggcatg
      841 cacagactcc agtgcggcag agttctgtca agggggaaag aaaagcacag acctaaggag
      901 cccatgaaaa tcagcgggtt ttcttcctag agcccgagcc cctggttttc tatctgttac
      961 acaatgtttg aggattagtc agaattactg tagtgtttgg ttgtacatag cagccctggc
     1021 atgagactga agaggaactc acacaactca tggagcatac ttagaaagag cactgagcca
     1081 gacgtggtgg ctcacaccta tagtcccagc acctgggagg ctgagggatt gctgtgagtg
     1141 tcgggccgcc ttgagctaca gaatgagctg agccaccagg gtaacagaga taagaacagg
     1201 catcggagcg ctgtgtgata cttcagaagt gctcacttga acaactcaat atatatatgt
     1261 ggtaaatact gtaatttgtg taaataaact tggttttgtt tgta
//
