LOCUS       NM_025895               4568 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus mediator complex subunit 28 (Med28), mRNA.
ACCESSION   NM_025895
VERSION     NM_025895.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Kolonay,D.W., Sattler,K.M., Strawser,C., Rafael-Fortney,J.,
            Mihaylova,M.M., Miller,K.E., Lepper,C. and Baskin,K.K.
  TITLE     Temporal regulation of the Mediator complex during muscle
            proliferation, differentiation, regeneration, aging, and disease
  JOURNAL   Front Cell Dev Biol 12, 1331563 (2024)
   PUBMED   38690566
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4568)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 4568)
  AUTHORS   Li,L., Walsh,R.M., Wagh,V., James,M.F., Beauchamp,R.L., Chang,Y.S.,
            Gusella,J.F., Hochedlinger,K. and Ramesh,V.
  TITLE     Mediator Subunit Med28 Is Essential for Mouse Peri-Implantation
            Development and Pluripotency
  JOURNAL   PLoS One 10 (10), e0140192 (2015)
   PUBMED   26445504
  REMARK    GeneRIF: These findings document the essential role of Med28 in the
            developing embryo as well as in acquisition and maintenance of
            pluripotency during reprogramming.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4568)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 4568)
  AUTHORS   Wiederhold,T., Lee,M.F., James,M., Neujahr,R., Smith,N., Murthy,A.,
            Hartwig,J., Gusella,J.F. and Ramesh,V.
  TITLE     Magicin, a novel cytoskeletal protein associates with the NF2 tumor
            suppressor merlin and Grb2
  JOURNAL   Oncogene 23 (54), 8815-8825 (2004)
   PUBMED   15467741
REFERENCE   7  (bases 1 to 4568)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   8  (bases 1 to 4568)
  AUTHORS   Liu,C., Zhang,L., Shao,Z.M., Beatty,P., Sartippour,M., Lane,T.F.,
            Barsky,S.H., Livingston,E. and Nguyen,M.
  TITLE     Identification of a novel endothelial-derived gene EG-1
  JOURNAL   Biochem Biophys Res Commun 290 (1), 602-612 (2002)
   PUBMED   11779215
REFERENCE   9  (bases 1 to 4568)
  AUTHORS   Suzuki,H., Fukunishi,Y., Kagawa,I., Saito,R., Oda,H., Endo,T.,
            Kondo,S., Bono,H., Okazaki,Y. and Hayashizaki,Y.
  TITLE     Protein-protein interaction panel using mouse full-length cDNAs
  JOURNAL   Genome Res 11 (10), 1758-1765 (2001)
   PUBMED   11591653
REFERENCE   10 (bases 1 to 4568)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DV653139.1 and AC092711.14.
            
            On May 7, 2009 this sequence version replaced NM_025895.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK154249.1, SRR7974084.19815.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-713               DV653139.1         1-713
            714-4568            AC092711.14        174605-178459
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 25.02 cM"
     gene            1..4568
                     /gene="Med28"
                     /gene_synonym="1500003D12Rik; Eg1; FKSG20; magicin"
                     /note="mediator complex subunit 28"
                     /db_xref="GeneID:66999"
                     /db_xref="MGI:MGI:1914249"
     exon            1..256
                     /gene="Med28"
                     /gene_synonym="1500003D12Rik; Eg1; FKSG20; magicin"
                     /inference="alignment:Splign:2.1.0"
     CDS             98..634
                     /gene="Med28"
                     /gene_synonym="1500003D12Rik; Eg1; FKSG20; magicin"
                     /note="endothelial-derived; mediator of RNA polymerase II
                     transcription, subunit 28 homolog"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 28"
                     /protein_id="NP_080171.1"
                     /db_xref="CCDS:CCDS19275.1"
                     /db_xref="GeneID:66999"
                     /db_xref="MGI:MGI:1914249"
                     /translation="MAASLGGMFTGQPPGPPPPPPGLPGQASLLQAAPGAPRPSNSTL
                     VDELESSFEACFASLVSQDYVNGTDQEEIRTGVDQCIQKFLDIARQTECFFLQKRLQL
                     SVQKPDQVIKEDVSELRSELQRKDALVQKHLTKLRHWQQVLEDINVQHKKPADMPQGS
                     LAFLEQASANIPAPLKQT"
     exon            257..323
                     /gene="Med28"
                     /gene_synonym="1500003D12Rik; Eg1; FKSG20; magicin"
                     /inference="alignment:Splign:2.1.0"
     exon            324..436
                     /gene="Med28"
                     /gene_synonym="1500003D12Rik; Eg1; FKSG20; magicin"
                     /inference="alignment:Splign:2.1.0"
     exon            437..4568
                     /gene="Med28"
                     /gene_synonym="1500003D12Rik; Eg1; FKSG20; magicin"
                     /inference="alignment:Splign:2.1.0"
     regulatory      867..872
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Med28"
                     /gene_synonym="1500003D12Rik; Eg1; FKSG20; magicin"
                     /note="hexamer: AGTAAA"
     polyA_site      885
                     /gene="Med28"
                     /gene_synonym="1500003D12Rik; Eg1; FKSG20; magicin"
                     /note="major polyA site"
     regulatory      2248..2253
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Med28"
                     /gene_synonym="1500003D12Rik; Eg1; FKSG20; magicin"
                     /note="hexamer: AATAAA"
     polyA_site      2267
                     /gene="Med28"
                     /gene_synonym="1500003D12Rik; Eg1; FKSG20; magicin"
     regulatory      4534..4539
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Med28"
                     /gene_synonym="1500003D12Rik; Eg1; FKSG20; magicin"
                     /note="hexamer: ATTAAA"
     polyA_site      4560
                     /gene="Med28"
                     /gene_synonym="1500003D12Rik; Eg1; FKSG20; magicin"
ORIGIN      
        1 ggcgaactgc ccgtcatgtg accaggcctg ccacgtgatc aggctgcccg cgcctgcgca
       61 cacaggcccg gtgtcgtttc cgcccgccgt tgcagacatg gcggcgtccc tgggagggat
      121 gttcactggg cagcctccag gtcccccgcc gccgccgccc gggctcccag gccaggcgtc
      181 gcttctgcaa gcagcccctg gggccccgag accatctaac agcactttgg tggacgagtt
      241 ggagtcatcc ttcgaggctt gctttgcttc ccttgtgagt caggattatg tcaatgggac
      301 tgatcaggaa gaaattcgaa ctggtgttga tcagtgtatc cagaagtttt tggacattgc
      361 aagacagaca gaatgttttt tcctacaaaa aaggttgcag ttatctgtcc agaaaccaga
      421 tcaagttatc aaagaggatg tctcggaact aaggagtgaa ctgcagcgga aggatgcgct
      481 ggtccagaag cacttgacaa agctgaggca ttggcagcag gtgctggagg acatcaatgt
      541 gcagcacaag aagccggctg acatgcctca gggatccttg gccttcctcg agcaggcgtc
      601 tgccaacatt cctgcacctc tgaagcagac ctaagcactc ctgcctgtct tgcagccttg
      661 cagtcttgcc agacacgttt ccttacagac ctggtgtgtt ggaaggactc tgttggataa
      721 tgagtttgta tcagtttctg tccctattta taaggtaggg tttcattatg aagccgggaa
      781 gggccttgaa tctatgatcc cctgcctcag ctcctctagt actctaaact cctgaatact
      841 ttataaaaaa cctctgtctt tgataaagta aagtttggct ttgtttgttg ttgttgtttg
      901 tattcatctt tagcactgtt tactccttag actgttagag tactcacaca cattcttatt
      961 tggcctttgc catttgtttt gacaacattc tgtgtcttca tctccctttt tttcttaagc
     1021 tatgaatttg gtgaaaaacc tgaaggaaac aatgtttagt agtttgagaa gccacgggat
     1081 gtacagtttg gttatcagcc ttaataggct acactagtgg taggtagtgt agacagagat
     1141 gtaaatgtgc tgttccaaaa ctgtgagctg cctcgtgagg aatctggcac ggcagtgtga
     1201 tgcttctagg gcggcacagg gcgagtgtat ttcacaggta gctcagaaga ggttagaata
     1261 tgctgctaat ggaagatact taccttcatg ctgggtgggt ttttgtctgg gctacgttcc
     1321 attctctgag tccttattca ctctgccttg agggcacaca catgaggact atggcatgct
     1381 gagtgctctt gggctgtgct gtgctggtca gggtaaggaa tggagtccca gggaaggctc
     1441 gggtgaggta tgaggtcaca agcctgcttg gattgtctat ttgtctctga gttttatttg
     1501 cttgtttttt gtttgtttgt cgtccccccc cccccccctt caaggccaag cacctctctt
     1561 tacccaaaac aaatactcca tacctcttga atagtcagtg catctccagc ttggctttgt
     1621 ccattttggt ttctggagcc ttagcccagg tcataagcat caccatccac tcactgccgg
     1681 cctagtctga gcactgccat ctggtctgtg gagcttcagc atgccccgtt gctgcgcctc
     1741 atctcagggc acagggtttt gtttgtaatt ttgtcaactt ccttaaattc tacatatgct
     1801 ttatgagtag cgactatgtt agttgctcta ttgtatgcct gttagagtta gggacttcct
     1861 tgattatgca aggtcttact agtttcatgt gtatgcttga ctgtatgata tctagttcct
     1921 ggaacaattt aagccagtct aaggccatca ggttccctta attagaatat ataaatgaaa
     1981 cagagtgttg aaaaatttag gctggcatgg tagcatttgg gaggtagagg aaggaagctc
     2041 aaagtgtaat ctcgtcttca gctacatagc aagtttgaag ctgttgtaga ttacatgaga
     2101 gagatctgcc cccgtcccct ctacttctgt caaaaaaaac cccaaaacaa aacaaccaac
     2161 caaacaaaac acttaattaa aacccatgca ttttctgaag tactgattga cagcttggct
     2221 gtttctggat ttcttggcta aaaactaaat aaactggtta cattttggct ggtttgtcaa
     2281 taatgaggag gatggtggaa atactgaaat cattcttggt gcttagtgaa aatattttta
     2341 agttggtcta catacaagtt actgtttatt ttaaattgaa ttcaacggtt aggttaaaat
     2401 aattttccca ttttgtcagt ttgtgtgtgt atagtattat ctgtgaacat gtttggggag
     2461 gggtatgtgt gtgtttttgt gaaggtcaaa gatccatgtg tgttagacat tagagatcca
     2521 ggtggtagct atttccagat tacctttcga gactcagttt ctcactgagt ttggccttgt
     2581 ttactggaca agactagctg gcaaaccaca gtgatcttcc tgcctctctc ctccttccaa
     2641 gctctaggat taagctactc tgctcggctt tttatcctgg tacaaccaca tgaactcagg
     2701 gcttcagtta cacacacgag ctttacttac agaaccatcc cccagctcca tctctctcct
     2761 cctaagtggc actttgctgg ctgttgaaca cagacattcc aagctgcatg tctttcaggg
     2821 gtttccgttg gtttgctgct cctatgtgta acctaatgga ttcaaggaag tttcctgctt
     2881 gtttcagttg cctacctgag taagaccagc ctttccatat gcatggatcc cagaccggtc
     2941 gccttctcta ggatgaagca ctgtggtgat ttctgccact tgcaactgtc tgtccttggc
     3001 tccttccctg tacctcaccc aaattctgtc ttaaacattt cccttgatct tttccagctc
     3061 cttattccgt ctccactctg ctccttcctc cacagctgta tttgtctgtc ctgcaattgt
     3121 gcttgtgatc atctttggca acagaccaga tggtacgttc acttccttag tttctataca
     3181 tgaacttagc ttcttgacta tccagttcca gtaccattgg ttgtgccagt cctgggtatt
     3241 agtttgcgta gcatgggtgc tacagtcagg tctgtttctg gaatcctcta gtggacagtg
     3301 gtcaggagca gtcagtcacc ctagtctaca ctgcagaact atccttgcac agagagcaca
     3361 cacctgccag tgtccagaag ctgacccagc agtgctccag ttactctgct gaggtaaaca
     3421 ggctcagaac accaaacctc atcgtcacct gaaacgatga tcaggtgaga caaatcactt
     3481 ataattctca ttggggcaca tatacgccca aaaccaagtc accaggtcat tcaaccacca
     3541 gacatcctgc tggatgaaat gagtgactgt gaccttggcc ccctttggtt aatatcaact
     3601 tgacaggatc tagtcccacc taggagacag acctcttagc atgtcttcga agaagtttat
     3661 gttggttgag gtatgtgggc agcagcatcc caagggctgg ggtccaatac tgaataaaac
     3721 atgagctagg cgtcattatt ctttgctctc tgcctcccat ctggacactg tgaccaacta
     3781 cttcagctct gtccttccca gccatggtgg actgaaccac caaaatcttg aaccatagta
     3841 aacttctctg agttcttttt tgtcagcagc aaggaaatgc agaaggctgc agaaaagcag
     3901 tcttgtgcct taccaagaat tacaatgctg agaaatgccc agatcagaaa aggcacaacc
     3961 acctgtatcg cccagtgctc actgcttatt gacaaacatt ggaacttatg gcaaacgaga
     4021 gtgggcattt cctatccaaa actccaaatg ctcccagatc taaacttttc caacaatgac
     4081 ctgacactga aaggggaaac ttgcatactt gatgcattga gtaaaaatgg aggtgtacca
     4141 agaaaagaat aaggtggata tagaaatgtc agggaatatg tttagttttt ggtcccatgt
     4201 ttaagataac ttgtgtagat tcatgaatct gaaaatatgc aaacatttat tttaagtctt
     4261 actggtgatt gaattcagga ccttgcacat gctaaccatg tgctatatac cactgctatg
     4321 cacttttaag cacttgtgga cccaattgtt aggtaggagg tgataaagca ataaaggctg
     4381 cttgctcatg agtggcatta gtacccttgt aaagaggcag ctctgttgca cacgaggccc
     4441 catagtggag acttacgaag aatgcttccc cactttcatc ttggtctctt acctgcagta
     4501 atgcatttct tttgtctgta tactctctcc atcattaaag ttttagtgta acagcctcaa
     4561 cactccag
//
