LOCUS       NM_026896               1276 bp    mRNA    linear   ROD 17-SEP-2024
DEFINITION  Mus musculus mediator complex subunit 27 (Med27), transcript
            variant 1, mRNA.
ACCESSION   NM_026896
VERSION     NM_026896.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Zhu,S., Chen,Z., Liu,C., Duong,J., Tran,T., Liang,Z., Fang,X. and
            Ouyang,K.
  TITLE     The essential role of MED27 in stabilizing the mediator complex for
            cardiac development and function
  JOURNAL   Life Sci 356, 123020 (2024)
   PUBMED   39209248
  REMARK    GeneRIF: The essential role of MED27 in stabilizing the mediator
            complex for cardiac development and function.
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Kolonay,D.W., Sattler,K.M., Strawser,C., Rafael-Fortney,J.,
            Mihaylova,M.M., Miller,K.E., Lepper,C. and Baskin,K.K.
  TITLE     Temporal regulation of the Mediator complex during muscle
            proliferation, differentiation, regeneration, aging, and disease
  JOURNAL   Front Cell Dev Biol 12, 1331563 (2024)
   PUBMED   38690566
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1276)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 1276)
  AUTHORS   Kagey,M.H., Newman,J.J., Bilodeau,S., Zhan,Y., Orlando,D.A., van
            Berkum,N.L., Ebmeier,C.C., Goossens,J., Rahl,P.B., Levine,S.S.,
            Taatjes,D.J., Dekker,J. and Young,R.A.
  TITLE     Mediator and cohesin connect gene expression and chromatin
            architecture
  JOURNAL   Nature 467 (7314), 430-435 (2010)
   PUBMED   20720539
  REMARK    Erratum:[Nature. 2011 Apr 14;472(7342):247]
REFERENCE   5  (bases 1 to 1276)
  AUTHORS   Krebs,A.R., Demmers,J., Karmodiya,K., Chang,N.C., Chang,A.C. and
            Tora,L.
  TITLE     ATAC and Mediator coactivators form a stable complex and regulate a
            set of non-coding RNA genes
  JOURNAL   EMBO Rep 11 (7), 541-547 (2010)
   PUBMED   20508642
REFERENCE   6  (bases 1 to 1276)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
REFERENCE   7  (bases 1 to 1276)
  AUTHORS   Moulson,C.L., Martin,D.R., Lugus,J.J., Schaffer,J.E., Lind,A.C. and
            Miner,J.H.
  TITLE     Cloning of wrinkle-free, a previously uncharacterized mouse
            mutation, reveals crucial roles for fatty acid transport protein 4
            in skin and hair development
  JOURNAL   Proc Natl Acad Sci U S A 100 (9), 5274-5279 (2003)
   PUBMED   12697906
REFERENCE   8  (bases 1 to 1276)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ072886.1, AK005251.1 and AI850753.1.
            
            On Mar 13, 2014 this sequence version replaced NM_026896.4.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC016537.1, AK005251.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                CJ072886.1         2-27
            27-1191             AK005251.1         2-1166
            1192-1276           AI850753.1         1-85                c
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 19.84 cM"
     gene            1..1276
                     /gene="Med27"
                     /gene_synonym="1500015J03Rik; 2310042P07Rik; Crsp8;
                     D2Ertd434e"
                     /note="mediator complex subunit 27"
                     /db_xref="GeneID:68975"
                     /db_xref="MGI:MGI:1916225"
     exon            1..238
                     /gene="Med27"
                     /gene_synonym="1500015J03Rik; 2310042P07Rik; Crsp8;
                     D2Ertd434e"
                     /inference="alignment:Splign:2.1.0"
     CDS             36..971
                     /gene="Med27"
                     /gene_synonym="1500015J03Rik; 2310042P07Rik; Crsp8;
                     D2Ertd434e"
                     /note="isoform a is encoded by transcript variant 1;
                     cofactor required for Sp1 transcriptional activation,
                     subunit 8; transcriptional co-activator CRSP8 homolog;
                     mediator of RNA polymerase II transcription subunit 27;
                     CRSP complex subunit 8"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 27 isoform a"
                     /protein_id="NP_081172.2"
                     /db_xref="CCDS:CCDS15853.1"
                     /db_xref="GeneID:68975"
                     /db_xref="MGI:MGI:1916225"
                     /translation="MADVLSVGVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKET
                     LEGREKAFIANFQDNLHSVNRDLNELERLSNLVGKPSENHPLHNSGLLSLDPVQDKTP
                     LYSQLLQAYKWSNKLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQ
                     YVDDVISRIDRMFPEMSIHLSRPNGTSAMLLVTLGKVLKVIVVMRSLFIDRTIVKGYN
                     ESVYTEDGKLDIWSKSSYQVFQKVTDHATTALLHYQLPQMPDVVVRSFMTWLRSYIKL
                     FQAPCQRCGKFLQDGLPPTWRDFRTLEAFHDTCRQ"
     exon            239..383
                     /gene="Med27"
                     /gene_synonym="1500015J03Rik; 2310042P07Rik; Crsp8;
                     D2Ertd434e"
                     /inference="alignment:Splign:2.1.0"
     exon            384..514
                     /gene="Med27"
                     /gene_synonym="1500015J03Rik; 2310042P07Rik; Crsp8;
                     D2Ertd434e"
                     /inference="alignment:Splign:2.1.0"
     exon            515..608
                     /gene="Med27"
                     /gene_synonym="1500015J03Rik; 2310042P07Rik; Crsp8;
                     D2Ertd434e"
                     /inference="alignment:Splign:2.1.0"
     exon            609..716
                     /gene="Med27"
                     /gene_synonym="1500015J03Rik; 2310042P07Rik; Crsp8;
                     D2Ertd434e"
                     /inference="alignment:Splign:2.1.0"
     exon            717..758
                     /gene="Med27"
                     /gene_synonym="1500015J03Rik; 2310042P07Rik; Crsp8;
                     D2Ertd434e"
                     /inference="alignment:Splign:2.1.0"
     exon            759..836
                     /gene="Med27"
                     /gene_synonym="1500015J03Rik; 2310042P07Rik; Crsp8;
                     D2Ertd434e"
                     /inference="alignment:Splign:2.1.0"
     exon            837..1260
                     /gene="Med27"
                     /gene_synonym="1500015J03Rik; 2310042P07Rik; Crsp8;
                     D2Ertd434e"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1239..1244
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Med27"
                     /gene_synonym="1500015J03Rik; 2310042P07Rik; Crsp8;
                     D2Ertd434e"
                     /note="hexamer: AATAAA"
     polyA_site      1258
                     /gene="Med27"
                     /gene_synonym="1500015J03Rik; 2310042P07Rik; Crsp8;
                     D2Ertd434e"
                     /note="major polyA site"
ORIGIN      
        1 gtggactctg gaaagccggc tgtgacagct gcaacatggc ggacgtgctg agcgttggtg
       61 tgaacctgga ggccttttcg caggctatta gcgccatcca ggcgctacgc tctagcgtga
      121 gtagggtctt cgactgcctt aaggatggca tgcgcaacaa agagacgctg gagggccgcg
      181 agaaggcctt catcgcgaac ttccaggaca acttgcattc cgtcaaccgg gacctcaatg
      241 aactggaacg tttgagcaac ctggtaggca agccgtctga gaaccaccct cttcataaca
      301 gtgggctgct aagcctggat cctgtgcagg acaaaacccc actctacagc cagcttctgc
      361 aagcatacaa atggtccaac aagctgcagt accatgcagg tctagcgtct ggccttttga
      421 accaacagtc cttgaagcgc tctgcaaatc agatgggtgt gtctgccaag cggagaccga
      481 aggcccagcc caccactctg gttctaccac ctcagtatgt cgatgacgtg atcagccgca
      541 ttgacagaat gtttccagag atgtccattc acttatcgag acccaatggg acttcagcaa
      601 tgcttctggt gaccttgggg aaggtgttga aagtcattgt ggtcatgagg agcctgttca
      661 tcgatcgcac gattgtgaag gggtataatg agagcgtcta cacagaggat ggaaagctgg
      721 acatatggtc caaatccagc taccaagtgt tccagaaggt gacagaccat gccaccactg
      781 ccctgcttca ctatcagtta ccccagatgc cagacgtcgt ggtcaggtcc ttcatgacct
      841 ggctgagaag ctacattaag ctgttccagg ccccgtgcca gcgctgtggg aagtttctac
      901 aagatggcct tcccccgacg tggagagact tccgaaccct ggaagctttc catgacacct
      961 gccgacagtg acagtgaccc cggccccagc acagggcagc ctgcagacag gctgccttcc
     1021 caaacagccc gacccattca ccacacagga gtctaccact gttctccctg gcagaactgt
     1081 cagctgcatg tcatgatggg gcatcacggg aggatggtct gccccttccc tgtgtgctgg
     1141 cccctgggtc tgctgctccc atgagctgtt aatggaatga acctcttcat tcttacattg
     1201 gttatgcttg gttggttttg taacttattt ttgtaaacaa taaagctttt gtaccctgga
     1261 aaaaaaaaaa aaaaaa
//
