LOCUS       NM_001331207            3720 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus mediator complex subunit 25 (Med25), transcript
            variant 3, mRNA.
ACCESSION   NM_001331207 XM_006541261
VERSION     NM_001331207.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Kolonay,D.W., Sattler,K.M., Strawser,C., Rafael-Fortney,J.,
            Mihaylova,M.M., Miller,K.E., Lepper,C. and Baskin,K.K.
  TITLE     Temporal regulation of the Mediator complex during muscle
            proliferation, differentiation, regeneration, aging, and disease
  JOURNAL   Front Cell Dev Biol 12, 1331563 (2024)
   PUBMED   38690566
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 3720)
  AUTHORS   Eguchi,T., Tezuka,T., Watanabe,Y., Inoue-Yamauchi,A., Sagara,H.,
            Ozawa,M. and Yamanashi,Y.
  TITLE     Calcium-binding protein 7 expressed in muscle negatively regulates
            age-related degeneration of neuromuscular junctions in mice
  JOURNAL   iScience 27 (2), 108997 (2024)
   PUBMED   38327785
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3720)
  AUTHORS   Saunders,J., Sikder,K., Phillips,E., Ishwar,A., Mothy,D.,
            Margulies,K.B. and Choi,J.C.
  TITLE     Med25 Limits Master Regulators That Govern Adipogenesis
  JOURNAL   Int J Mol Sci 24 (7), 6155 (2023)
   PUBMED   37047128
  REMARK    GeneRIF: Med25 Limits Master Regulators That Govern Adipogenesis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3720)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   6  (bases 1 to 3720)
  AUTHORS   Han,E.H., Rha,G.B. and Chi,Y.I.
  TITLE     MED25 is a mediator component of HNF4alpha-driven transcription
            leading to insulin secretion in pancreatic beta-cells
  JOURNAL   PLoS One 7 (8), e44007 (2012)
   PUBMED   22952853
  REMARK    GeneRIF: the identification of MED25 as one of the HNF4alpha
            binding partners in pancreatic ss-cells leading to insulin
            secretion which is impaired in MODY patients
REFERENCE   7  (bases 1 to 3720)
  AUTHORS   Nakamura,Y., Yamamoto,K., He,X., Otsuki,B., Kim,Y., Murao,H.,
            Soeda,T., Tsumaki,N., Deng,J.M., Zhang,Z., Behringer,R.R.,
            Crombrugghe,Bd., Postlethwait,J.H., Warman,M.L., Nakamura,T. and
            Akiyama,H.
  TITLE     Wwp2 is essential for palatogenesis mediated by the interaction
            between Sox9 and mediator subunit 25
  JOURNAL   Nat Commun 2, 251 (2011)
   PUBMED   21427722
REFERENCE   8  (bases 1 to 3720)
  AUTHORS   Leal,A., Huehne,K., Bauer,F., Sticht,H., Berger,P., Suter,U.,
            Morera,B., Del Valle,G., Lupski,J.R., Ekici,A., Pasutto,F.,
            Endele,S., Barrantes,R., Berghoff,C., Berghoff,M., Neundorfer,B.,
            Heuss,D., Dorn,T., Young,P., Santolin,L., Uhlmann,T.,
            Meisterernst,M., Sereda,M.W., Sereda,M., Stassart,R.M., Meyer zu
            Horste,G., Nave,K.A., Reis,A. and Rautenstrauss,B.
  TITLE     Identification of the variant Ala335Val of MED25 as responsible for
            CMT2B2: molecular data, functional studies of the SH3 recognition
            motif and correlation between wild-type MED25 and PMP22 RNA levels
            in CMT1A animal models
  JOURNAL   Neurogenetics 10 (4), 275-287 (2009)
   PUBMED   19290556
  REMARK    GeneRIF: Data show that Med25 is coordinately expressed with Pmp22
            gene dosage and expression in transgenic mice and rats, and suggest
            a potential role of this protein in the molecular etiology of
            Charcot-Marie-Tooth disease.
            Erratum:[Neurogenetics. 2009 Oct;10(4):375-376. doi:
            10.1007/s10048-009-0213-1.. Sereda, Michael [corrected to Sereda,
            Michael Werner]; Stassart, Ruth Martha [added]. PMID: 25488817]
REFERENCE   9  (bases 1 to 3720)
  AUTHORS   Lee,H.K., Park,U.H., Kim,E.J. and Um,S.J.
  TITLE     MED25 is distinct from TRAP220/MED1 in cooperating with CBP for
            retinoid receptor activation
  JOURNAL   EMBO J 26 (15), 3545-3557 (2007)
   PUBMED   17641689
REFERENCE   10 (bases 1 to 3720)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC158231.2.
            
            On Dec 30, 2022 this sequence version replaced NM_001331207.1.
            
            Sequence Note:.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK090026.1, SRR13422597.595493.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-188               AC158231.2         164930-165117       c
            189-234             AC158231.2         164798-164843       c
            235-359             AC158231.2         164482-164606       c
            360-458             AC158231.2         158871-158969       c
            459-579             AC158231.2         158483-158603       c
            580-742             AC158231.2         158038-158200       c
            743-873             AC158231.2         157779-157909       c
            874-961             AC158231.2         157532-157619       c
            962-1155            AC158231.2         157262-157455       c
            1156-1284           AC158231.2         156818-156946       c
            1285-1370           AC158231.2         155798-155883       c
            1371-1428           AC158231.2         155667-155724       c
            1429-1536           AC158231.2         155458-155565       c
            1537-1725           AC158231.2         153622-153810       c
            1726-1797           AC158231.2         153266-153337       c
            1798-2016           AC158231.2         152951-153169       c
            2017-2194           AC158231.2         152626-152803       c
            2195-3720           AC158231.2         149464-150989       c
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 28.95 cM"
     gene            1..3720
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /note="mediator complex subunit 25"
                     /db_xref="GeneID:75613"
                     /db_xref="MGI:MGI:1922863"
     exon            1..188
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..2460
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /note="isoform 3 is encoded by transcript variant 3;
                     mediator of RNA polymerase II transcription subunit 25;
                     mediator of RNA polymerase II transcription, subunit 9
                     homolog"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 25 isoform 3"
                     /protein_id="NP_001318136.1"
                     /db_xref="CCDS:CCDS90228.1"
                     /db_xref="GeneID:75613"
                     /db_xref="MGI:MGI:1922863"
                     /translation="MVPGSEGPARAGGLVADVVFVIEGTANLGPYFEELRKHYLLPAI
                     EYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIK
                     FMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTT
                     YSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQPADVSQDPR
                     HMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPG
                     NLSAAQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPG
                     APKPPPASQPSLVSTVAPGPVLAAPAQPGAPSLAGTVTPGGVNGPSAAQLGGPALGGQ
                     QSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIM
                     QLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEV
                     RVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGMGAQQAPPV
                     LGPILEEQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAA
                     PGPPPSGPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQ
                     SMGQPQLGPQLLHPPPAQSWPTQLPQRAPLPVAKRKREGEGRVFREKWERDYFFVEVK
                     SMPTCLICKKNVSVLKEYNLKRHYESQHSKSYDQYTAQSRDTLLQELKRALRASEALE
                     SQE"
     exon            189..234
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /inference="alignment:Splign:2.1.0"
     exon            235..359
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /inference="alignment:Splign:2.1.0"
     exon            360..458
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /inference="alignment:Splign:2.1.0"
     exon            459..579
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /inference="alignment:Splign:2.1.0"
     exon            580..742
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /inference="alignment:Splign:2.1.0"
     exon            743..873
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /inference="alignment:Splign:2.1.0"
     exon            874..961
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1155
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1284
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1285..1370
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1428
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1429..1536
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1537..1725
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..1797
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1798..2016
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2017..2194
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2195..3720
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3698..3703
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /note="hexamer: AGTAAA"
     polyA_site      3720
                     /gene="Med25"
                     /gene_synonym="2610034E13Rik; 2610529E18Rik; ESTM2"
                     /note="major polyA site"
ORIGIN      
        1 gttcgtcccg ggacggcgac ggcgtcagcg gcgctggcgt cggcggcgcg gggcatggtc
       61 cccggatccg aaggcccggc gcgggccggg ggcctggtcg ccgatgtggt gtttgtgatc
      121 gaggggacag caaacctggg gccgtacttc gaggagctcc gcaagcacta cctgctgcct
      181 gccatagagt acttcaacgg gggaccccct gcagagacgg actttggggg agactatggt
      241 ggaacccagt acagccttgt ggtgttcaac accgtggact gcgctccaga gtcctatgta
      301 caatgtcacg cacctaccag cagtgcctat gagtttgtca cctggctcga tggcatcaag
      361 ttcatgggcg gtggtggcga gagctgtagt ctcatcgctg aaggtctgag cactgccctg
      421 cagctgttcg atgactttaa gaagatgcgt gaacagattg gccagaccca ccgagtctgt
      481 ctcctcatct gcaactcacc cccgtacctc ctgcccgccg tggagagcac cacctactca
      541 ggctgcacta ccgagagcct ggtgcagaaa ataggagagc gaggcatcca cttctccatc
      601 gtttctccga gaaagctgcc agccctccgg cttctgtttg agaaggcggc tcctcctgcc
      661 ctactggagc ctttgcagca accagcagac gtcagccagg accccaggca catggtgctt
      721 gtgcgtgggc tcgtgctgcc tgttggtggc agctcgacct caggctctct gcagaccaag
      781 caagcagtcc ccttgccacc agctccagct tctgctgcca cgctgtcagc agcgcccccg
      841 caggctctgc cccctgtccc accacagtac caggttcctg ggaacctaag tgctgctcag
      901 gtggctgcgc agaatgctgt ggaggctgcc aagagccaga aggccgggct gggcccacgc
      961 ttctcaccca tcaaccctct ccagcaagct gctccaggag tggggccccc cttcagccag
     1021 gccccagcac ccccattggc tcctgtgccc cctggagctc caaagccacc tcctgcctca
     1081 cagcctagcc tagtctccac cgtggccccc ggaccagtcc tggcagctcc tgcgcagcct
     1141 ggggcgccgt ctctggcagg cactgtaacc ccaggtgggg tcaatggtcc ttcagcagcc
     1201 cagcttgggg gtcctgcact tggtgggcag cagtcagtat ccaacaagct cctagcctgg
     1261 agtggagtcc tcgagtggca ggagaaaccc aagcctgcat ctgtggatgc caacaccaag
     1321 ctgacgaggt ctctgccttg ccaggtctat gtgaaccatg gagaaaacct gaagactgag
     1381 cagtggcccc agaaactgat catgcagctt atccctcagc agctgctgac caccctgggt
     1441 ccgctgttcc ggaactcaag aatggttcag ttccacttca ccaacaagga cctggagtcc
     1501 ctcaaaggcc tctaccgcat catgggcaat ggctttgcgg gctgcgtgca tttcccgcac
     1561 acggccccat gcgaggtgcg cgtgcttatg ctcctgtact cgtctaagaa gaagatcttc
     1621 atgggcctca tcccctacga ccagagcggc ttcgtcaatg gcatccgcca ggtcatcacc
     1681 aaccacaagc aggtccagca gcagaagctg gagcaacagc gcgggatggg ggcacagcag
     1741 gcacctccag tcctgggccc cattctggag gagcaagcga ggcccccgca gaatctacta
     1801 cagctccgtg caccacagcc tcagcctcag ggtgccgtgg gagcttctgc ggctacaggg
     1861 cagccccagc cccaaggtgc tacccaggcc cccacaggcg cacctcaagg tcctcctgga
     1921 gcagcccctg gcccacctcc ttctggaccc atcctgcggc cccagaaccc tggggccaac
     1981 ccccagctgc ggagccttct ccttaaccca gcaccgcccc agactggggt gcccccgccc
     2041 caggcctccc tacaccacct acagcctcca ggggccccta cattgctgcc accacatcag
     2101 agcatggggc agccccagct ggggccgcag ctcctgcatc ctccacctgc ccagtcatgg
     2161 cccacacagc ttccccagcg ggcgccactg ccagtggcca aacgaaagag agaaggagag
     2221 ggccgcgtgt ttcgagaaaa gtgggagcga gactatttct ttgtggaagt gaagagcatg
     2281 cctacgtgct taatatgcaa aaaaaacgtg tcagtgctga aagagtacaa cctgaagcgc
     2341 cactatgagt cccagcacag taagagctac gatcagtaca cagcgcagag ccgcgacaca
     2401 ctcctgcagg aactgaaaag ggccctgaga gccagcgagg cgctggagag ccaggaatag
     2461 ccatgcagtg cagtggaaag aactcccgtg ttctgaagag ctgtcatcaa ggctgtcctg
     2521 tgagcctgac aagcgtctgc aggctgaagc acacaagagt gaggccttcc ggatccacac
     2581 agaaactctg agcacatggc tagctttatt tattggtggt ggtggcggca atatccatgc
     2641 aaccgaggca gtctgtgagg tggcaccaag ccagcccctg actttgaagt gacttgctcc
     2701 tgtgcatgca aacacatcag aattcataag cacaactaca gtcccatcat ggtgaccagg
     2761 ctgtccatca tgggggctgt tccatacctc gatcccaagt ggcactgttt gcaaggaccc
     2821 aggtcaggtc ctagtctcag taaccaggaa ttgagatgct caaagaaaca ccatgacgcc
     2881 ttcctcgact ctgccattcc tgtacttgaa ctgagccaat tgcctgagac caggacatgc
     2941 aggaaatctg aactggtatg tttggttcat ttcaaagtgt gcagagccgt gccctggttc
     3001 cctggaggac agttagaaac ttttggttga agttcccacc cttgtgaaca ctgaatctgt
     3061 ctcagaaggg atattcacga acagctcctg aatgtatatg actgtggtgt ggtcctggca
     3121 ggcgcatgcc tttgcgagga acagtgggac tgcctgcctt tggtgcagct tgtttctgga
     3181 aatgagaggc ggccaggacc agtcacccac actgtgagac ccaagaccaa gtcccctgag
     3241 ggccatactc tacttgaacc tgagctaaca ctggctagct cgcccttacg atgatgtgaa
     3301 cgcagacccc ggtccgagtt ggcagtgcga cagggtgctt gcaattctgc aagatggcaa
     3361 ggttcctttg cctctgccca gcagtgtcct gagtggctgc ccacgcttgt gacagtcatt
     3421 gttttccgtt acatacctaa atcaaaaact ccattttaag gattaaggtt gacatttcac
     3481 ctgccgtctc tgtcccagcc aatgccagca ccgacatctg ggtgaagaag ggcaggtgtc
     3541 aaccttccgc tcaaaaggca caagaaaatt acaagagaga aattttaata actaaatatt
     3601 tctatttttc aatttgtatt ttttcaattt tgaatttaaa gtatgaactc tagtatcatc
     3661 cgtgtttgtg ttgtattcca tgcgttcacc atagctcagt aaagatccaa tttgattatc
//
