LOCUS       NM_001166416            4825 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Mus musculus mediator complex subunit 23 (Med23), transcript
            variant 1, mRNA.
ACCESSION   NM_001166416
VERSION     NM_001166416.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Fang,Y., Yuan,C., Li,C., Lu,C., Yu,W. and Wang,G.
  TITLE     The Mediator Med23 controls a transcriptional switch for muscle
            stem cell proliferation and differentiation in muscle regeneration
  JOURNAL   Cell Rep 43 (5), 114177 (2024)
   PUBMED   38691453
  REMARK    GeneRIF: The Mediator Med23 controls a transcriptional switch for
            muscle stem cell proliferation and differentiation in muscle
            regeneration.
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Kolonay,D.W., Sattler,K.M., Strawser,C., Rafael-Fortney,J.,
            Mihaylova,M.M., Miller,K.E., Lepper,C. and Baskin,K.K.
  TITLE     Temporal regulation of the Mediator complex during muscle
            proliferation, differentiation, regeneration, aging, and disease
  JOURNAL   Front Cell Dev Biol 12, 1331563 (2024)
   PUBMED   38690566
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4825)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 4825)
  AUTHORS   Fu,X., Liu,S., Cao,D., Li,C., Ji,H. and Wang,G.
  TITLE     Med23 deficiency reprograms the tumor microenvironment to promote
            lung tumorigenesis
  JOURNAL   Br J Cancer 130 (5), 716-727 (2024)
   PUBMED   38195889
  REMARK    GeneRIF: Med23 deficiency reprograms the tumor microenvironment to
            promote lung tumorigenesis.
REFERENCE   5  (bases 1 to 4825)
  AUTHORS   Yang,Y., Li,C., Chen,Z., Zhang,Y., Tian,Q., Sun,M., Zhang,S., Yu,M.
            and Wang,G.
  TITLE     An intellectual disability-related MED23 mutation dysregulates gene
            expression by altering chromatin conformation and enhancer
            activities
  JOURNAL   Nucleic Acids Res 51 (5), 2137-2150 (2023)
   PUBMED   36718943
  REMARK    GeneRIF: An intellectual disability-related MED23 mutation
            dysregulates gene expression by altering chromatin conformation and
            enhancer activities.
            Erratum:[Nucleic Acids Res. 2023 Mar 21;51(5):2499. doi:
            10.1093/nar/gkad132.. PMID: 36806874]
REFERENCE   6  (bases 1 to 4825)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 4825)
  AUTHORS   Stevens,J.L., Cantin,G.T., Wang,G., Shevchenko,A., Shevchenko,A.
            and Berk,A.J.
  TITLE     Transcription control by E1A and MAP kinase pathway via Sur2
            mediator subunit
  JOURNAL   Science 296 (5568), 755-758 (2002)
   PUBMED   11934987
REFERENCE   8  (bases 1 to 4825)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
REFERENCE   9  (bases 1 to 4825)
  AUTHORS   Shi,J.D., Wang,C.Y., Marron,M.P., Ruan,Q.G., Huang,Y.Q.,
            Detter,J.C. and She,J.X.
  TITLE     Chromosomal localization and complete genomic sequence of the
            murine autoimmune regulator gene (Aire)
  JOURNAL   Autoimmunity 31 (1), 47-53 (1999)
   PUBMED   10593569
REFERENCE   10 (bases 1 to 4825)
  AUTHORS   Tchernev,V.T., Barbosa,M.D., Detter,J.C., Patel,T.D., Achey,K.,
            Wakeland,E.K., Gueorguieva,R.V., Yang,M.C., Gossler,A. and
            Kingsmore,S.F.
  TITLE     Genetic mapping of 20 novel expressed sequence tags from
            midgestation mouse embryos suggests chromosomal clustering
  JOURNAL   Genomics 40 (1), 170-174 (1997)
   PUBMED   9070936
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC159502.12.
            
            On Apr 9, 2022 this sequence version replaced NM_001166416.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            CCDS Note: The upstream start codon may likely be used, however,the
            coding region was updated to begin from a downstream start codon
            based on currently available conservation data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13861889.128614.1,
                                           SRR17253011.2661608.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-112               AC159502.12        139259-139370
            113-144             AC159502.12        139678-139709
            145-232             AC159502.12        139888-139975
            233-357             AC159502.12        142697-142821
            358-469             AC159502.12        143794-143905
            470-568             AC159502.12        144820-144918
            569-670             AC159502.12        146164-146265
            671-740             AC159502.12        147613-147682
            741-853             AC159502.12        148897-149009
            854-949             AC159502.12        151778-151873
            950-967             AC159502.12        152428-152445
            968-1168            AC159502.12        157624-157824
            1169-1312           AC159502.12        160343-160486
            1313-1458           AC159502.12        161738-161883
            1459-1704           AC159502.12        162839-163084
            1705-1847           AC159502.12        164930-165072
            1848-2022           AC159502.12        166409-166583
            2023-2186           AC159502.12        167634-167797
            2187-2311           AC159502.12        169920-170044
            2312-2517           AC159502.12        171227-171432
            2518-2698           AC159502.12        171517-171697
            2699-2869           AC159502.12        172885-173055
            2870-3086           AC159502.12        173485-173701
            3087-3323           AC159502.12        175046-175282
            3324-3489           AC159502.12        176406-176571
            3490-3562           AC159502.12        177159-177231
            3563-3777           AC159502.12        177857-178071
            3778-3898           AC159502.12        179022-179142
            3899-4030           AC159502.12        179958-180089
            4031-4825           AC159502.12        181887-182681
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10"
     gene            1..4825
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /note="mediator complex subunit 23"
                     /db_xref="GeneID:70208"
                     /db_xref="MGI:MGI:1917458"
     exon            1..112
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     CDS             74..4195
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cofactor required for Sp1 transcriptional activation,
                     subunit 3 (130kD); mediator of RNA polymerase II
                     transcription subunit 23; mSur-2; protein sur-2 homolog;
                     CRSP complex subunit 3; cofactor required for Sp1
                     transcriptional activation subunit 3"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 23 isoform 1"
                     /protein_id="NP_001159888.2"
                     /db_xref="CCDS:CCDS48522.1"
                     /db_xref="GeneID:70208"
                     /db_xref="MGI:MGI:1917458"
                     /translation="METQLQSIFEEVVKTEIIEEAFPGMFMDTPEDEKTKLISCLAAF
                     RQFWSGLSQESHEQCVQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRMVCESLI
                     NSDSLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKAILEKILTIPNTVSSAVVQQL
                     LAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARI
                     NSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQ
                     PYSRDMVCNMLGLNKQTLNIAQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQ
                     LLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQK
                     NALADFLPVMKLFDLLYPEKECIPVPDINKPQSTHAFAMTCIWIHLNRKAQNGDSTLQ
                     IPIPHSLKLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGN
                     GIMRVPLPGTSCLASASVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHTKSSVALA
                     PALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSHRMHHIQPH
                     YRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVL
                     SAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMNFTPHNWASHTLSC
                     FPAPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMTDENEIITQFSVQGFPPLFLCL
                     LWKMLLETDHISQIGYKVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEIL
                     NDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENS
                     PEHWLQSDWHTKHMSYHKKYPEKLYFEGLAEQVDPPVPIQSPYLPIYFGNVCLRFLPV
                     FDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMCLRNRDHLKR
                     KLVHAIIGSLKDNRPQGWCLSDTYLKHAMNAREDNPWVPEDSYYCKLIGRLVDTMAGK
                     SPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRE
                     NITAWMNAIGLIITALPEPYWIVLHDRIVNVISSSSLTSETEWVGYPFRLFDFTACHQ
                     SYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEALLPVVKTEFQLLYVYHLVGPFL
                     QRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQV
                     EKIICNLKPALKLRLRFITHISKMEPAVPPQALNSGSPAPQSNQVPASLPVTQ"
     exon            113..144
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            145..232
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            233..357
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            358..469
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            470..568
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            569..670
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            671..740
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            741..853
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            854..949
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            950..967
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1168
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..1312
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            1313..1458
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..1704
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            1705..1847
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            1848..2022
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            2023..2186
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            2187..2311
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            2312..2517
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            2518..2698
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            2699..2869
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            2870..3086
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            3087..3323
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            3324..3489
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            3490..3562
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            3563..3777
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            3778..3898
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            3899..4030
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     exon            4031..4825
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4591..4596
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /note="hexamer: AATAAA"
     polyA_site      4625
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /note="major polyA site"
     regulatory      4804..4809
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /note="hexamer: AATAAA"
     polyA_site      4825
                     /gene="Med23"
                     /gene_synonym="130kDa; 3000002A17Rik; Crsp3; ESTM7;
                     mKIAA1216; sno; snouty; Sur2"
                     /note="major polyA site"
ORIGIN      
        1 ccattctttc ccgaccctgg ctctcacccg tctgtcgccg ggttccccgg gagcccgggg
       61 ctcgatggta ccgatggaga cgcaactgca gagcattttc gaggaggtgg tgaaaacaga
      121 aattatagaa gaggccttcc caggaatgtt tatggatacc cccgaggatg aaaaaacaaa
      181 actaattagc tgcttggcgg ccttccggca gttttggagt ggactttctc aggagtctca
      241 tgaacagtgt gttcagtgga ttgttaaatt tattcatggc caacacagcc ccaaaagaat
      301 ttctttcctc tatgactgct tagcaatggc agttgagact ggtctccttc cacccaggat
      361 ggtttgtgaa tctttgataa actctgactc tcttgaatgg gaaagaacac agctttgggc
      421 cttaacattt aagctggttc ggaaaatcat tggaggagtg gattacaagg gtgttcgaga
      481 ccttctcaaa gcgattttgg agaaaatctt gacaatccca aatacagtga gctctgctgt
      541 tgtccagcag cttctggcag caagagaggt tatagcttat atcttagaac gaaatgcctg
      601 cctattgccg gcctactttg cagtcacaga gatcagaaaa ctatatcctg agggaaaact
      661 tccacactgg ttactaggaa acttggtatc agactttgtg gataccttca ggcccacagc
      721 aaggattaac tccatttgtg gccgatgcag tcttctgcca gttgtgaaca actcgggagc
      781 catctgtaac tcatggaaac tggaccctgc aactctgcga tttcctttga aaggcctttt
      841 gccatatgat aaggatctgt ttgaaccgca gactgctttg ttgagatatg tactggagca
      901 accttattcc agggatatgg tctgcaatat gctaggttta aataagcaga ccttgaacat
      961 tgctcagcac aagcagcgct gccctgtgct ggaggaccag ttggtggacc tggtggtgta
     1021 cgccatggag aggtcagaga ccgaggagaa gtttgacgat gggggaacca gccagctcct
     1081 gtggcagcac ctttccagcc agctcatttt ctttgtgctt ttccagtttg ccagtttccc
     1141 acatatggtc ctctctctcc accagaagtt agcagggcga ggactgatta aagggcgaga
     1201 ccatctgatg tgggtgctgc tgcagttcat ttctggaagt attcagaaaa atgccctcgc
     1261 tgacttcctc cctgtcatga agctctttga cctgctgtac ccggagaagg agtgtatccc
     1321 agttcctgat attaacaaac cccagtcaac gcacgccttt gcaatgactt gtatttggat
     1381 tcatcttaat agaaaagctc aaaatggcga ctccacgctc cagatcccaa taccccattc
     1441 cctgaagctg caccatgagt tcctccagca gagtctgaga aataaaagct tacagatgaa
     1501 tgactataag attgccctgc tatgtaacgc gtattccaca aactcagagt gttttacgtt
     1561 acctatggga gctctggtag aaactattta tggaaatgga attatgagag tgcctctccc
     1621 tggaacgagc tgtttggcct cagcatcagt gactccctta ccgatgaacc tcctggattc
     1681 actgacggtg catgccaaga tgagccttat tcacagcatt gcaaccagag tgataaaact
     1741 ggctcatacg aaatccagtg ttgcgctggc tccagcccta gtggaaactt acagccgttt
     1801 actggtctac atggaaattg agtctttggg catcaaagga tttatcagtc agctcctgcc
     1861 cactgtcttc aagtcccacg cctggggcat cctgcacaca ctgctggaga tgttcagcca
     1921 ccgaatgcac cacattcagc cccactaccg agttcagctc ctgagccatc tccacacact
     1981 ggctgcagtc gcacagacca accagaacca gctccatctg tgtgtggaga gcactgcact
     2041 gaggctcatc acagccctgg gaagctcaga ggtccagccg cagttcacgc gtttcctcaa
     2101 tgatcccaaa acagtgctgt ccgccgaatc tgaagagctg aaccgagcct tgatcctgac
     2161 cttagccaga gcaacccacg tcacagattt ttttacagga tctgattcaa tacagggaac
     2221 ttggtgtaaa gatatccttc agaccatcat gaactttact cctcataact gggcttcaca
     2281 tacccttagc tgttttccag cccctctgca ggctttcttc aagcaaaata acgtgcccca
     2341 ggaaagccgt tttaatctga agaagaatgt ggaagaagag tacaggaagt ggaagtcaat
     2401 gactgatgaa aacgagatca tcacccagtt ctctgtgcag ggcttccctc cactcttcct
     2461 ctgtctcctc tggaagatgc tcctggagac ggatcatatt agtcagatcg gctacaaggt
     2521 cctggagagg attggggcca gggccctggt ggcccatgtg agaacatttg cagatttcct
     2581 ggtgtatgag ttctctacgt cagcaggagg tcagcaactc aataagtgca ttgaaattct
     2641 caacgacatg gtgtggaagt acaacattgt cacgctggac agactcattc tctgcctggc
     2701 tatgcgtagt catgaaggaa atgaagccca ggtttgttat ttcataattc agttgctgtt
     2761 gctcaaacca aatgacttca gaaaccgagt aagtgacttt gtgaaggaga attccccaga
     2821 gcactggctc cagagtgact ggcacaccaa gcacatgagc taccacaaga aatatcccga
     2881 gaagctgtat tttgaaggcc tggcagagca ggttgaccct cctgtaccaa tccagtcccc
     2941 ttatctgccc atctactttg gaaatgtgtg tctccggttc cttccagtat ttgatatagt
     3001 aatccacagg tttttagagt tgcttccagt gtccaaatca ctagagactc tcctggatca
     3061 cctagggggc ttatataaat tccatgaccg gccagtgact tatctgtata atactttgca
     3121 ctattatgaa atgtgcctga gaaaccgtga ccatctcaaa cgaaaacttg tccatgcaat
     3181 cattggctcc ctcaaagata accggccaca gggctggtgt ctcagtgata cctacctgaa
     3241 gcatgctatg aatgcacggg aggacaaccc ctgggttccg gaggactcct actactgcaa
     3301 gctgattggc cgactggtag acaccatggc gggcaagtct ccaggaccct tcccaaactg
     3361 tgactggaga ttcaatgagt ttcccaaccc cgctgcccac gctctgcatg tgacgtgtgt
     3421 ggaactcatg gccttggcgg ttccaggcaa agatgtgggg aatgcccttc tcaatgtcgt
     3481 cctgaaaagc cagcccttag tgccaaggga gaacattaca gcgtggatga atgcaattgg
     3541 tctgatcatc acggccctcc cagagccgta ctggattgtg cttcatgacc gaattgtgaa
     3601 tgtgatcagc agttctagcc tgacgtcaga gacagagtgg gtcggttacc cgttccgcct
     3661 cttcgacttc accgcttgtc accagtccta ctcggagatg agctgcagct atacgttagc
     3721 tctagcccat gccgtgtggc accactccag catcgggcag ctctcgctta tcccaaagtt
     3781 tctcactgag gcgcttctcc cggtggtgaa gactgagttc cagttgctct acgtgtacca
     3841 tcttgtgggg ccatttctac agaggttcca acaggagaga acccggtgca tgattgagat
     3901 tggcgtggca ttttatgaca tgctgttgaa tgtagaccag tgcagcacgc acttaaatta
     3961 catggacccc atctgcgact tcctgtatca tatgaagtat atgtttactg gtgacagtgt
     4021 gaaagagcaa gtagagaaga ttatctgtaa tttaaagccg gctttaaagc ttcgtcttcg
     4081 attcatcacg cacattagca agatggagcc ggcagtgcca ccccaggccc tcaacagcgg
     4141 gtctccagca cctcagtcta accaggtgcc agcatctctg ccggtgactc agtgaagcca
     4201 gaggcactgt gactgatgca gaggagtgga ctcacgtcta aatctgaaca ccgtgctagt
     4261 aaaacgagag cagccaccat gttcagattg gtttattctg attcaaatac tgaatccctc
     4321 tccttgtcta tgttttgtct ctaagagata agtaagttac atgttgttca tattcatatc
     4381 actttattca aatccctagg aatgtgggcg tttctatact caagtggctt ttactaggca
     4441 ctctcagact tcctgtctta gccattttta aattattgta agtaacatct ttacatcttg
     4501 tataccttac cttgaagatg cgacacatgt gaaatggcca ttacaaaccc ttttgtacat
     4561 aaatgtcctt ccaaatagag cctttttatt aataaagggc cttgtacttc acttggaaca
     4621 aaggacgttt catactcttg tctttggttt tttttaattg cctataaaat ttttattgta
     4681 aattttaaat ttccaagtgg ttccaagtaa gggatgccaa gtttctacca acgtaggtta
     4741 tgggagtttt gcttcgctct gagctctctg tatgaaatga aaccatcaga aagttagaaa
     4801 tgaaataaag ccgtgctcag acgtc
//
