LOCUS       NM_001033908            1367 bp    mRNA    linear   ROD 18-JUN-2024
DEFINITION  Mus musculus mediator complex subunit 22 (Med22), transcript
            variant 2, mRNA.
ACCESSION   NM_001033908
VERSION     NM_001033908.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Kolonay,D.W., Sattler,K.M., Strawser,C., Rafael-Fortney,J.,
            Mihaylova,M.M., Miller,K.E., Lepper,C. and Baskin,K.K.
  TITLE     Temporal regulation of the Mediator complex during muscle
            proliferation, differentiation, regeneration, aging, and disease
  JOURNAL   Front Cell Dev Biol 12, 1331563 (2024)
   PUBMED   38690566
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1367)
  AUTHORS   Rodriguez,P.Q., Unnersjo-Jess,D., Zambrano,S.S., Guo,J.,
            Moller-Hackbarth,K., Blom,H., Jahnukainen,T., Ebarasi,L. and
            Patrakka,J.
  TITLE     Inactivation of mediator complex protein 22 in podocytes results in
            intracellular vacuole formation, podocyte loss and premature death
  JOURNAL   Sci Rep 10 (1), 20037 (2020)
   PUBMED   33208756
  REMARK    GeneRIF: Inactivation of mediator complex protein 22 in podocytes
            results in intracellular vacuole formation, podocyte loss and
            premature death.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1367)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1367)
  AUTHORS   Tomomori-Sato,C., Sato,S., Parmely,T.J., Banks,C.A., Sorokina,I.,
            Florens,L., Zybailov,B., Washburn,M.P., Brower,C.S., Conaway,R.C.
            and Conaway,J.W.
  TITLE     A mammalian mediator subunit that shares properties with
            Saccharomyces cerevisiae mediator subunit Cse2
  JOURNAL   J Biol Chem 279 (7), 5846-5851 (2004)
   PUBMED   14638676
REFERENCE   6  (bases 1 to 1367)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
REFERENCE   7  (bases 1 to 1367)
  AUTHORS   Magoulas,C. and Fried,M.
  TITLE     The Surf-6 gene of the mouse surfeit locus encodes a novel
            nucleolar protein
  JOURNAL   DNA Cell Biol 15 (4), 305-316 (1996)
   PUBMED   8639267
REFERENCE   8  (bases 1 to 1367)
  AUTHORS   Garson,K., Duhig,T., Armes,N., Colombo,P. and Fried,M.
  TITLE     Surf5: a gene in the tightly clustered mouse surfeit locus is
            highly conserved and transcribed divergently from the rpL7A (Surf3)
            gene
  JOURNAL   Genomics 30 (2), 163-170 (1995)
   PUBMED   8586415
REFERENCE   9  (bases 1 to 1367)
  AUTHORS   Yu,L.C., Chen,J.L., Tsai,W.B. and Chen,Y.H.
  TITLE     Primary structure and characterization of an androgen-stimulated
            autoantigen purified from mouse seminal-vesicle secretion
  JOURNAL   Biochem J 296 (Pt 3) (Pt 3), 571-576 (1993)
   PUBMED   8280054
REFERENCE   10 (bases 1 to 1367)
  AUTHORS   Huxley,C. and Fried,M.
  TITLE     The mouse surfeit locus contains a cluster of six genes associated
            with four CpG-rich islands in 32 kilobases of genomic DNA
  JOURNAL   Mol Cell Biol 10 (2), 605-614 (1990)
   PUBMED   2300057
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL773563.12 and BG805496.1.
            
            Transcript Variant: This variant (2) uses alternate splice sites in
            the coding region, compared to variant 1. The resulting isoform (2)
            is longer and has a unique C-terminus when it is compared to
            isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FO719623.1, FO720089.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-350               AL773563.12        113824-114173       c
            351-737             BG805496.1         213-599
            738-1367            AL773563.12        108798-109427       c
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 19.09 cM"
     gene            1..1367
                     /gene="Med22"
                     /gene_synonym="Surf-5; Surf5"
                     /note="mediator complex subunit 22"
                     /db_xref="GeneID:20933"
                     /db_xref="MGI:MGI:98446"
     exon            1..350
                     /gene="Med22"
                     /gene_synonym="Surf-5; Surf5"
                     /inference="alignment:Splign:2.1.0"
     CDS             228..830
                     /gene="Med22"
                     /gene_synonym="Surf-5; Surf5"
                     /note="isoform 2 is encoded by transcript variant 2;
                     mediator of RNA polymerase II transcription subunit 22;
                     surfeit locus protein 5; surfeit gene 5"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 22 isoform 2"
                     /protein_id="NP_001029080.1"
                     /db_xref="CCDS:CCDS15813.1"
                     /db_xref="GeneID:20933"
                     /db_xref="MGI:MGI:98446"
                     /translation="MAQQRALPQSKETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIED
                     ETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRN
                     QQLRALQEECDRKLITLRDEVSIDLYELEEEYYSSSSSLCEANDLPLCEAYWRLDLDA
                     DSADGLSAPLLASPETGAGPLQSAAPVHSHGGGPGPTEHT"
     exon            351..431
                     /gene="Med22"
                     /gene_synonym="Surf-5; Surf5"
                     /inference="alignment:Splign:2.1.0"
     exon            432..640
                     /gene="Med22"
                     /gene_synonym="Surf-5; Surf5"
                     /inference="alignment:Splign:2.1.0"
     exon            641..1367
                     /gene="Med22"
                     /gene_synonym="Surf-5; Surf5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1350..1355
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Med22"
                     /gene_synonym="Surf-5; Surf5"
                     /note="hexamer: AATAAA"
     polyA_site      1367
                     /gene="Med22"
                     /gene_synonym="Surf-5; Surf5"
                     /note="major polyA site"
ORIGIN      
        1 aggcgggcgg gccctggcac cggaccgcag gctccgagag gcggcgggag gcgccaggag
       61 tgaacggaag ccgcgggaag gagcgtgcgg gtgctttgtt ccctcgccgt aagggcctgc
      121 tgccgggcgt gtgttcgtgg acggagctgt tgtatcggaa atcccggtcc ccagccctca
      181 gtactcggtt cgggccggac cagactggtt ctcgtccagg gcccgccatg gcccagcaga
      241 gagcccttcc ccagagcaag gagacgctgc tgcagtcata caacaagcgg ctcaaagacg
      301 acatcaagtc catcatggac aacttcaccg agatcatcaa gaccgccaag attgaggatg
      361 agacgcaggt gtctcgggct actcagggcg aacaggacaa ttatgagatg cacgttcgag
      421 cagccaacat cgttcgagct ggtgagtcac tgatgaagct ggtatccgat ctcaagcagt
      481 ttctgatcct caacgacttc ccgtctgtga atgaagccat cgaccagcgt aaccagcagc
      541 ttcgagccct gcaggaggag tgtgaccgca agctcatcac cctgcgggac gaggtctcca
      601 ttgacctata tgagctggag gaggagtact actcgtccag ctcaagtctc tgcgaagcta
      661 atgacctgcc tctgtgtgaa gcttactgga ggctggacct cgacgcagat tctgctgatg
      721 gcctctcagc ccctcttctg gcgtccccgg agactggtgc tggtccctta cagtctgcag
      781 cccctgtcca ctcccatggt ggtggccctg gccctacaga acacacctga gcctcttggg
      841 gtaccatcgc agggtagccc tccctagggc ctagtttatc agaaaggaag tgctgtggat
      901 acccagcaca gcctagcttt tttcagtttt agctcccttg gggactttgg gatcaggagc
      961 cactgtagcc tcaggaacct gtccaggcca gggccagttc cccgtgatgg ttggggaatg
     1021 ctgtccagtt atttacagca ggtggctccc ttcctgactg ccagtggaag tttgtcccat
     1081 tgaagacctt gaatagggag ggtggttggt gataggaaga cagcctcagt ttgtttagaa
     1141 ttgtgaggta ttcagtggac cttagtcaag tccagttacc catccttagg ttcaggttct
     1201 atagagagcc ctgtccactg cctgctgcct ggagcataaa tgtctctgcc acagctgact
     1261 gtccaagatt gtgtgagagg cagctagcca gggtgtcttc ctggattctc ccaccatttc
     1321 cttctgctac ttttgagatg agactgttga ataaaacatt agctttt
//
