LOCUS       NM_020048               1884 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus mediator complex subunit 20 (Med20), transcript
            variant 1, mRNA.
ACCESSION   NM_020048
VERSION     NM_020048.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Kolonay,D.W., Sattler,K.M., Strawser,C., Rafael-Fortney,J.,
            Mihaylova,M.M., Miller,K.E., Lepper,C. and Baskin,K.K.
  TITLE     Temporal regulation of the Mediator complex during muscle
            proliferation, differentiation, regeneration, aging, and disease
  JOURNAL   Front Cell Dev Biol 12, 1331563 (2024)
   PUBMED   38690566
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1884)
  AUTHORS   Weng,L., Tang,W.S., Wang,X., Gong,Y., Liu,C., Hong,N.N., Tao,Y.,
            Li,K.Z., Liu,S.N., Jiang,W., Li,Y., Yao,K., Chen,L., Huang,H.,
            Zhao,Y.Z., Hu,Z.P., Lu,Y., Ye,H., Du,X., Zhou,H., Li,P. and
            Zhao,T.J.
  TITLE     Surplus fatty acid synthesis increases oxidative stress in
            adipocytes and lnduces lipodystrophy
  JOURNAL   Nat Commun 15 (1), 133 (2024)
   PUBMED   38168040
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1884)
  AUTHORS   Tan,C., Zhu,S., Chen,Z., Liu,C., Li,Y.E., Zhu,M., Zhang,Z.,
            Zhang,Z., Zhang,L., Gu,Y., Liang,Z., Boyer,T.G., Ouyang,K.,
            Evans,S.M. and Fang,X.
  TITLE     Mediator complex proximal Tail subunit MED30 is critical for
            Mediator core stability and cardiomyocyte transcriptional network
  JOURNAL   PLoS Genet 17 (9), e1009785 (2021)
   PUBMED   34506481
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1884)
  AUTHORS   Tang,W.S., Weng,L., Wang,X., Liu,C.Q., Hu,G.S., Yin,S.T., Tao,Y.,
            Hong,N.N., Guo,H., Liu,W., Wang,H.R. and Zhao,T.J.
  TITLE     The Mediator subunit MED20 organizes the early adipogenic complex
            to promote development of adipose tissues and diet-induced obesity
  JOURNAL   Cell Rep 36 (1), 109314 (2021)
   PUBMED   34233190
  REMARK    GeneRIF: The Mediator subunit MED20 organizes the early adipogenic
            complex to promote development of adipose tissues and diet-induced
            obesity.
REFERENCE   6  (bases 1 to 1884)
  AUTHORS   Krebs,A.R., Demmers,J., Karmodiya,K., Chang,N.C., Chang,A.C. and
            Tora,L.
  TITLE     ATAC and Mediator coactivators form a stable complex and regulate a
            set of non-coding RNA genes
  JOURNAL   EMBO Rep 11 (7), 541-547 (2010)
   PUBMED   20508642
REFERENCE   7  (bases 1 to 1884)
  AUTHORS   Malik,S., Guermah,M., Yuan,C.X., Wu,W., Yamamura,S. and Roeder,R.G.
  TITLE     Structural and functional organization of TRAP220, the
            TRAP/mediator subunit that is targeted by nuclear receptors
  JOURNAL   Mol Cell Biol 24 (18), 8244-8254 (2004)
   PUBMED   15340084
  REMARK    Erratum:[Mol Cell Biol. 2022 Nov 17;42(11):e0035922. doi:
            10.1128/mcb.00359-22.. PMID: 36255214]
REFERENCE   8  (bases 1 to 1884)
  AUTHORS   Sato,S., Tomomori-Sato,C., Banks,C.A., Sorokina,I., Parmely,T.J.,
            Kong,S.E., Jin,J., Cai,Y., Lane,W.S., Brower,C.S., Conaway,R.C. and
            Conaway,J.W.
  TITLE     Identification of mammalian Mediator subunits with similarities to
            yeast Mediator subunits Srb5, Srb6, Med11, and Rox3
  JOURNAL   J Biol Chem 278 (17), 15123-15127 (2003)
   PUBMED   12584197
REFERENCE   9  (bases 1 to 1884)
  AUTHORS   Nelson,P.S., Pritchard,C., Abbott,D. and Clegg,N.
  TITLE     The human (PEDB) and mouse (mPEDB) Prostate Expression Databases
  JOURNAL   Nucleic Acids Res 30 (1), 218-220 (2002)
   PUBMED   11752298
REFERENCE   10 (bases 1 to 1884)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK041624.1 and AK043396.1.
            
            On Jul 18, 2018 this sequence version replaced NM_020048.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ245617.1, AK043396.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                AK041624.1         2-12
            12-1884             AK043396.1         2-1874
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 23.66 cM"
     gene            1..1884
                     /gene="Med20"
                     /gene_synonym="1110011O05Rik; 2410115I17Rik; Trfp"
                     /note="mediator complex subunit 20"
                     /db_xref="GeneID:56771"
                     /db_xref="MGI:MGI:1929648"
     exon            1..108
                     /gene="Med20"
                     /gene_synonym="1110011O05Rik; 2410115I17Rik; Trfp"
                     /inference="alignment:Splign:2.1.0"
     CDS             95..733
                     /gene="Med20"
                     /gene_synonym="1110011O05Rik; 2410115I17Rik; Trfp"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin specific protease 49; mediator of RNA polymerase
                     II transcription subunit 20; Trf (TATA binding
                     protein-related factor)-proximal protein homolog"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 20 isoform 1"
                     /protein_id="NP_064432.1"
                     /db_xref="CCDS:CCDS28851.1"
                     /db_xref="GeneID:56771"
                     /db_xref="MGI:MGI:1929648"
                     /translation="MGVTCVSQMPVAEGKSLQQTVELLTKKLEMLGAEKQGTFCVDCE
                     TYHTAASTLGSQGQAGKLMYVMHNSEYPLSCFALFENGPCLIADTNFDVLMVKLKGFF
                     QSAKASKIETRGTRYQYCDFLVKVGTVTMGPSARGISVEVEYGPCVVASDCWSLLLEF
                     LQSFLGSHAPGAPTVFGNRHDAVYGPADTMIQYMELFNKIRKQQQVPVAGIR"
     exon            109..263
                     /gene="Med20"
                     /gene_synonym="1110011O05Rik; 2410115I17Rik; Trfp"
                     /inference="alignment:Splign:2.1.0"
     exon            264..517
                     /gene="Med20"
                     /gene_synonym="1110011O05Rik; 2410115I17Rik; Trfp"
                     /inference="alignment:Splign:2.1.0"
     exon            518..1884
                     /gene="Med20"
                     /gene_synonym="1110011O05Rik; 2410115I17Rik; Trfp"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1864..1869
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Med20"
                     /gene_synonym="1110011O05Rik; 2410115I17Rik; Trfp"
                     /note="hexamer: AATAAA"
     polyA_site      1884
                     /gene="Med20"
                     /gene_synonym="1110011O05Rik; 2410115I17Rik; Trfp"
                     /note="major polyA site"
ORIGIN      
        1 gactgcttcc tgtgtttagg gggccgccgt tcgcgtgaag gtctggagtc tctgagttcg
       61 agggtttgga ggcgccacct tttgatcctt cgctatggga gtgacgtgtg tgtcccagat
      121 gcctgtggct gagggcaaga gtctccagca gacagtggag ctcctcacca agaaactgga
      181 gatgcttgga gcagagaaac aagggacatt ttgtgtggac tgtgagactt accacacagc
      241 tgcctctacc ctgggcagcc aaggccaggc tgggaagctg atgtacgtga tgcataactc
      301 tgagtaccca ctgagctgct ttgccctctt tgagaatggc ccttgcctca ttgctgacac
      361 caacttcgat gtgcttatgg tgaagctcaa gggcttcttc cagagtgcca aggccagcaa
      421 gattgagacc cggggcactc gctaccagta ttgtgacttc ctggtgaagg tgggcacggt
      481 cacaatgggg cccagtgccc gtggcatctc tgtggaggtg gagtatggcc cttgtgtggt
      541 ggctagtgac tgctggagcc tcctgcttga gttcctacag agttttctag ggagccacgc
      601 accaggggca cccacagtat ttgggaacag acacgatgcg gtctatgggc cagcagatac
      661 catgatacag tacatggaac tcttcaacaa gatccgaaaa cagcagcaag tgccagtggc
      721 tgggatccgt tagtgactgg cagctgccca cctgctgagt tgtcaccagg gtaatgccca
      781 ggcccacgtg ctggactcgg tcctgggccc ctggcccagc tggttcccag ctgtagcttg
      841 tgttccgggg ctttggactt ccctcctgag cccatcacag ctggttttta cttcatgctt
      901 taactggatt tggctggtcc tcaagaatgg taactgtgga gaatggaaaa gctgtagggc
      961 aaaaggtagg acagggttgt cgctgttggc actggagctg tgtgtgtccc aggtgctgag
     1021 ggtttgttat cggctggacg cctgtgctgt atggggctcc ccttcgcttg aggatgcggc
     1081 tggcctctcc tggagatggt taggtggcag gactggattg ttggctgcta gagtgtcgct
     1141 ttcattcttt ggacactgat gtcttctcat actgttttaa attctgtgac cctgtaggcc
     1201 aggccgctgt agcagaacca aaagcagagg actcttactg cacagtttat ttttcattaa
     1261 tagttcacag gcattcagtt cccagggcaa catgaactcc acagcgttac caggaacttg
     1321 gcttcctatg tcttactgtt ccttatatca tactgtccca aaatgctttc cttcctacag
     1381 gatgctcagt agctctgcac ccacagtcca ctgaggagag cgagattgag agggatggtt
     1441 ttcagagttg ctcaaagcac tgtggacaca tgttcctgac actcaacact caggtttttc
     1501 agggtgcctc ctaggttgtc ttatcttgct tgagccttga attcactgac actattactt
     1561 acctgcctca cctactacag agacatttaa cacaaagaca ggctcgggaa agctgttaat
     1621 ctacaaagtg taggctgtca gttaggatcc tgtcctcaga gcacagctag gcagacttgc
     1681 cagcgcatct gggtagtggc tctgaggtac tggtcattct tgtctcctag ctttagctgg
     1741 caggacagct catcacgcct tcctgctgtg gctcagttat ccggttgctc ctgttgcttc
     1801 ctcttgcttt ggttgttagt catttactgt tgttccgtaa acctgaggtg ttttacatat
     1861 cgtaataaag ttttaccagc atta
//
