LOCUS       NM_198107               3201 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Mus musculus mediator complex subunit 16 (Med16), transcript
            variant 1, mRNA.
ACCESSION   NM_198107
VERSION     NM_198107.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Kolonay,D.W., Sattler,K.M., Strawser,C., Rafael-Fortney,J.,
            Mihaylova,M.M., Miller,K.E., Lepper,C. and Baskin,K.K.
  TITLE     Temporal regulation of the Mediator complex during muscle
            proliferation, differentiation, regeneration, aging, and disease
  JOURNAL   Front Cell Dev Biol 12, 1331563 (2024)
   PUBMED   38690566
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 3201)
  AUTHORS   Tan,C., Zhu,S., Chen,Z., Liu,C., Li,Y.E., Zhu,M., Zhang,Z.,
            Zhang,Z., Zhang,L., Gu,Y., Liang,Z., Boyer,T.G., Ouyang,K.,
            Evans,S.M. and Fang,X.
  TITLE     Mediator complex proximal Tail subunit MED30 is critical for
            Mediator core stability and cardiomyocyte transcriptional network
  JOURNAL   PLoS Genet 17 (9), e1009785 (2021)
   PUBMED   34506481
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3201)
  AUTHORS   Sekine,H., Okazaki,K., Ota,N., Shima,H., Katoh,Y., Suzuki,N.,
            Igarashi,K., Ito,M., Motohashi,H. and Yamamoto,M.
  TITLE     The Mediator Subunit MED16 Transduces NRF2-Activating Signals into
            Antioxidant Gene Expression
  JOURNAL   Mol Cell Biol 36 (3), 407-420 (2015)
   PUBMED   26572828
  REMARK    GeneRIF: NRF2-dependent phosphorylation of the RNA polymerase II
            C-terminal domain was absent in Med16-deficient cells, suggesting
            that MED16 serves as a conduit to transmit NRF2-activating signals
            to RNA polymerase II.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3201)
  AUTHORS   Krebs,A.R., Demmers,J., Karmodiya,K., Chang,N.C., Chang,A.C. and
            Tora,L.
  TITLE     ATAC and Mediator coactivators form a stable complex and regulate a
            set of non-coding RNA genes
  JOURNAL   EMBO Rep 11 (7), 541-547 (2010)
   PUBMED   20508642
REFERENCE   6  (bases 1 to 3201)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
REFERENCE   7  (bases 1 to 3201)
  AUTHORS   Pritsker,M., Doniger,T.T., Kramer,L.C., Westcot,S.E. and
            Lemischka,I.R.
  TITLE     Diversification of stem cell molecular repertoire by alternative
            splicing
  JOURNAL   Proc Natl Acad Sci U S A 102 (40), 14290-14295 (2005)
   PUBMED   16183747
REFERENCE   8  (bases 1 to 3201)
  AUTHORS   Malik,S., Guermah,M., Yuan,C.X., Wu,W., Yamamura,S. and Roeder,R.G.
  TITLE     Structural and functional organization of TRAP220, the
            TRAP/mediator subunit that is targeted by nuclear receptors
  JOURNAL   Mol Cell Biol 24 (18), 8244-8254 (2004)
   PUBMED   15340084
  REMARK    Erratum:[Mol Cell Biol. 2022 Nov 17;42(11):e0035922. doi:
            10.1128/mcb.00359-22.. PMID: 36255214]
REFERENCE   9  (bases 1 to 3201)
  AUTHORS   Ito,M., Okano,H.J., Darnell,R.B. and Roeder,R.G.
  TITLE     The TRAP100 component of the TRAP/Mediator complex is essential in
            broad transcriptional events and development
  JOURNAL   EMBO J 21 (13), 3464-3475 (2002)
   PUBMED   12093747
REFERENCE   10 (bases 1 to 3201)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC151846.3.
            
            On Mar 30, 2022 this sequence version replaced NM_198107.2.
            
            Transcript Variant: This variant (1) encodes the longest protein
            (isoform 1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC057056.1, AK042340.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-106               AC151846.3         91076-91181
            107-272             AC151846.3         92343-92508
            273-380             AC151846.3         92853-92960
            381-550             AC151846.3         93065-93234
            551-982             AC151846.3         96571-97002
            983-1088            AC151846.3         97471-97576
            1089-1244           AC151846.3         99747-99902
            1245-1453           AC151846.3         100590-100798
            1454-1660           AC151846.3         100896-101102
            1661-1871           AC151846.3         101553-101763
            1872-2005           AC151846.3         102301-102434
            2006-2198           AC151846.3         103048-103240
            2199-2415           AC151846.3         103435-103651
            2416-2499           AC151846.3         104289-104372
            2500-3201           AC151846.3         104589-105290
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 39.72 cM"
     gene            1..3201
                     /gene="Med16"
                     /gene_synonym="95kDa; A630083L04; DRIP92; Thrap5; Trap95"
                     /note="mediator complex subunit 16"
                     /db_xref="GeneID:216154"
                     /db_xref="MGI:MGI:2158394"
     exon            1..106
                     /gene="Med16"
                     /gene_synonym="95kDa; A630083L04; DRIP92; Thrap5; Trap95"
                     /inference="alignment:Splign:2.1.0"
     exon            107..272
                     /gene="Med16"
                     /gene_synonym="95kDa; A630083L04; DRIP92; Thrap5; Trap95"
                     /inference="alignment:Splign:2.1.0"
     CDS             140..2626
                     /gene="Med16"
                     /gene_synonym="95kDa; A630083L04; DRIP92; Thrap5; Trap95"
                     /note="isoform 1 is encoded by transcript variant 1;
                     thyroid hormone receptor associated protein 5; mediator of
                     RNA polymerase II transcription subunit 16; thyroid
                     hormone receptor-associated protein, 95-kD subunit;
                     vitamin D3 receptor-interacting protein complex 92 kDa
                     component; thyroid hormone receptor-associated protein
                     complex 95 kDa component"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 16 isoform 1"
                     /protein_id="NP_932775.3"
                     /db_xref="CCDS:CCDS23996.2"
                     /db_xref="GeneID:216154"
                     /db_xref="MGI:MGI:2158394"
                     /translation="MDLAYVCEWEKWAKSTYCPSLPLACAWSCRNLIAFTTDLRNDDQ
                     DLTHMIHILDTEHPWEVHSVSSGHSEAITCLEWDQSGSRLLSADADGQIKCWSMADHL
                     ANSWESSVGSQVEGDPIVALSWLHNGVKLALHVEKSGASSFGEKFSRVKFSPSLTLFG
                     GKPMEGWIAVTVSGLVTVSLLKPSGQVLTSTESLCRLRGRVALADIAFTGGGNIVVAA
                     ADGSSASPVKFYKVCVSVVSEKCRIDTEILPSLFMRCTTDPNRKDRFPAITHLKFLAR
                     DMSEQVLLCASSQTSSLVECWSLRKEGLPVNNIFQQISPVVGDKQPMILKWRILSATN
                     DLDRVSAVALPKLPISLTNTDLKVASDTQFYPGLGLALAFQDGSVHMVHRLSLQTMAV
                     FYSSAPRSLDEPALKRPRTTCPAVHFKAMQLSWTSLALVGIDNHGKLSMLRISPSLGH
                     PLEPKLALQHLLFLLEYCMVTGYDWWDILLHVQPGMVQSLVERLHEEYTRQKPALQQV
                     LSTRILAMKASLCKLSPCTVARVCDYHTKLFLMAITSTLKSLLRPHFLNTPDKSPGDR
                     LAEICAKITDVDIDKVMINLKTEEFVLDMNTLQALQQLLQWVGDFVLYLLVSLPNQGS
                     PLRPGHSFLRDGTSLGMLRELMVVIRIWGLLKPSCLPVYTATSDTQDSMSLLFRLLTK
                     LWICCRDEGAASEPDEGLVDECCLLPSQLLVPNLDWLPASDGLVSRLQPKQPLRLRFG
                     RAPTLPSSTSTLQLDGLTRAPGQPKIDHLRRLHLGAYPTEECKACTRCGCVTMLKSPN
                     KTTAVTQWEQRWIKNCLCGGLWRRVPLSCS"
     exon            273..380
                     /gene="Med16"
                     /gene_synonym="95kDa; A630083L04; DRIP92; Thrap5; Trap95"
                     /inference="alignment:Splign:2.1.0"
     exon            381..550
                     /gene="Med16"
                     /gene_synonym="95kDa; A630083L04; DRIP92; Thrap5; Trap95"
                     /inference="alignment:Splign:2.1.0"
     exon            551..982
                     /gene="Med16"
                     /gene_synonym="95kDa; A630083L04; DRIP92; Thrap5; Trap95"
                     /inference="alignment:Splign:2.1.0"
     exon            983..1088
                     /gene="Med16"
                     /gene_synonym="95kDa; A630083L04; DRIP92; Thrap5; Trap95"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1244
                     /gene="Med16"
                     /gene_synonym="95kDa; A630083L04; DRIP92; Thrap5; Trap95"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1453
                     /gene="Med16"
                     /gene_synonym="95kDa; A630083L04; DRIP92; Thrap5; Trap95"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1660
                     /gene="Med16"
                     /gene_synonym="95kDa; A630083L04; DRIP92; Thrap5; Trap95"
                     /inference="alignment:Splign:2.1.0"
     exon            1661..1871
                     /gene="Med16"
                     /gene_synonym="95kDa; A630083L04; DRIP92; Thrap5; Trap95"
                     /inference="alignment:Splign:2.1.0"
     exon            1872..2005
                     /gene="Med16"
                     /gene_synonym="95kDa; A630083L04; DRIP92; Thrap5; Trap95"
                     /inference="alignment:Splign:2.1.0"
     exon            2006..2198
                     /gene="Med16"
                     /gene_synonym="95kDa; A630083L04; DRIP92; Thrap5; Trap95"
                     /inference="alignment:Splign:2.1.0"
     exon            2199..2415
                     /gene="Med16"
                     /gene_synonym="95kDa; A630083L04; DRIP92; Thrap5; Trap95"
                     /inference="alignment:Splign:2.1.0"
     exon            2416..2499
                     /gene="Med16"
                     /gene_synonym="95kDa; A630083L04; DRIP92; Thrap5; Trap95"
                     /inference="alignment:Splign:2.1.0"
     exon            2500..3201
                     /gene="Med16"
                     /gene_synonym="95kDa; A630083L04; DRIP92; Thrap5; Trap95"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3180..3185
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Med16"
                     /gene_synonym="95kDa; A630083L04; DRIP92; Thrap5; Trap95"
                     /note="hexamer: AATAAA"
     polyA_site      3201
                     /gene="Med16"
                     /gene_synonym="95kDa; A630083L04; DRIP92; Thrap5; Trap95"
                     /note="major polyA site"
ORIGIN      
        1 gtgcgagggg gccggcgcgg gcctagagat ggcggcgtcg gatcccgggc gttggggcga
       61 gtgtggaccg cggccgccgg gccgactggg cgcccggggc ggacaggagc cagggaccgt
      121 gctgggtgcg gtgggtatga tggacctggc ctatgtgtgc gaatgggaga agtgggccaa
      181 gagcacgtac tgcccgtcgc tgcccctggc ttgtgcctgg tcctgcagga acctcattgc
      241 cttcaccaca gacctacgca atgatgacca ggatctgaca cacatgatcc atatcctaga
      301 cacggaacac ccctgggaag tgcactctgt cagctctggc cacagcgagg ccatcacctg
      361 cctggagtgg gaccagtcag ggtcccggct actgtctgct gatgctgatg gacagatcaa
      421 atgctggagc atggctgacc acctggccaa cagctgggag agctcggtgg gcagccaggt
      481 ggagggggac cccatcgtgg ccctgtcctg gctgcacaac ggtgtaaagc tggccctgca
      541 tgtggagaag tctggtgcct ctagcttcgg agagaagttc tcacgtgtga agttctcccc
      601 gtccctcacg ctgttcggtg gcaagccaat ggagggttgg attgcagtga cagtcagtgg
      661 cctggtcact gtgtcccttc tgaagcccag cgggcaggtg ctgacgtcca cggagagcct
      721 gtgccggctg cgtggtagag tggcgctggc tgacatcgcc ttcacgggcg gtgggaacat
      781 tgtggtggct gctgcagatg gcagcagcgc gtcacccgtg aagttctata aggtgtgcgt
      841 cagcgtggtc agcgagaagt gccgcatcga cacggagatc ctgccctcac tgtttatgcg
      901 ctgcaccacc gaccccaacc gcaaagacag gttccccgcc atcacacacc tcaagttcct
      961 ggcgcgagac atgtctgagc aggtgctcct gtgtgcgtcc agccagacca gcagcctggt
     1021 ggaatgctgg tcccttcgga aagagggcct tcctgtgaac aatatcttcc agcagatctc
     1081 gcctgtggtt ggtgacaaac agcccatgat cctcaagtgg cgaatcctgt cggccaccaa
     1141 tgacctggac cgtgtatccg cggtggcact gccgaaactg cccatctcac tcaccaacac
     1201 tgacctcaag gtggccagtg acacccagtt ctatccaggc cttggcttag cgctggcctt
     1261 ccaggatggc agcgtgcaca tggtgcaccg actgtccctg cagaccatgg cggtgttcta
     1321 cagctcagcc ccgcgctcgc tggatgagcc ggccctaaag cgcccacgca ccacatgccc
     1381 tgccgtacac tttaaagcca tgcagctgtc ctggacttcg ctggccctcg tgggcatcga
     1441 taaccatggg aagctcagca tgctgcgaat ctctccatcc ctgggccacc cactggagcc
     1501 caagctggcc ctgcagcacc tgcttttcct gctggaatac tgcatggtga ccggctatga
     1561 ctggtgggac atcctgctac acgtgcagcc cggcatggtg cagagcctgg tggagcggct
     1621 gcatgaggag tacactcgcc agaagcccgc cctgcaacag gtcctctcca ctcggatcct
     1681 ggccatgaag gcttcactgt gcaagctgtc accctgcaca gtggctcgtg tgtgtgacta
     1741 ccacaccaag ctgttcctca tggccatcac gtccacccta aagtcgctgc tgcgcccaca
     1801 cttcctcaac acccctgaca agagccctgg ggaccgcctg gccgagatct gcgccaagat
     1861 caccgatgtt gacatcgaca aagtcatgat caacctgaag actgaagagt tcgtccttga
     1921 catgaacact ttgcaggcgc tgcagcagtt gctacagtgg gtgggagact tcgtgctcta
     1981 cctcctggtc agcctgccca accagggctc cccgctaagg ccgggccaca gcttcctccg
     2041 agatggtacc tccctgggca tgctgcgaga gttgatggtt gtcatccgaa tctggggcct
     2101 gctgaagccc agctgtctgc ccgtctacac agccacctcg gacacccagg acagcatgtc
     2161 cctgctcttc cgactgctca ccaaactgtg gatctgctgc cgtgatgagg gcgcagccag
     2221 tgaaccagat gagggtttgg tggatgaatg ctgcctgctg cctagccagc tgctggtccc
     2281 caacttggac tggcttcccg ccagtgatgg cctggtcagt cgcctgcagc ccaaacagcc
     2341 cctgcgcctg cgcttcggca gagcgcccac tctgcccagc agcacctcca ccctgcagct
     2401 ggacggcctc accagggccc ctggccagcc caagatcgac cacctccgga ggctacacct
     2461 gggcgcctac cccaccgagg agtgcaaagc ctgcaccagg tgtggctgtg tcaccatgct
     2521 gaagtctccc aacaagacaa ccgctgtgac acagtgggaa cagcggtgga tcaagaattg
     2581 cctgtgtggt gggctgtggc ggcgagtgcc tctcagctgt tcctgagcca cagcccacca
     2641 gcctgcgtat gtacaaccag tctctgaaca ttgacattgc tgtgctcact gaggcacaag
     2701 tacccaccaa tggcccctgg gtctgcccct tctagggaca tgcggcatct ctagggcctg
     2761 aagtcccatg gtcatatcca agccagtgtg aaggagctgg gctgtgtatt catcctttaa
     2821 aagatccaca gtggccgggc gtggtggcgc acgcctttaa tcccagcact cgggaggcag
     2881 aggcaggtgg atttctgagt tcgaggccag cctggtctac aaagtgagtt ccaggacagc
     2941 cagggctaca cagagaaacc ctgtctcgaa aaaacaaaac aaaaaaaaaa aaagattcac
     3001 agtacataaa gccccttgtc ctgcttgggg tcgtctgcct cagttttctt gtcctgcttg
     3061 gggtcgtctg cctcagtttt cttgtcctgc ttggggtcgt ctgcctcagt tttcttgtcc
     3121 tgcttggggt cgtctgcctc agttttcttt gggtgccggt gggaggggaa gactggccca
     3181 ataaagttct ctgatgcact a
//
