LOCUS       NM_033609               3413 bp    mRNA    linear   ROD 17-DEC-2024
DEFINITION  Mus musculus mediator complex subunit 15 (Med15), transcript
            variant 1, mRNA.
ACCESSION   NM_033609
VERSION     NM_033609.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Kadhim,A.Z., Vanderkruk,B., Mar,S., Dan,M., Zosel,K., Xu,E.E.,
            Spencer,R.J., Sasaki,S., Cheng,X., Sproul,S.L.J., Speckmann,T.,
            Nian,C., Cullen,R., Shi,R., Luciani,D.S., Hoffman,B.G., Taubert,S.
            and Lynn,F.C.
  TITLE     Transcriptional coactivator MED15 is required for beta cell
            maturation
  JOURNAL   Nat Commun 15 (1), 8711 (2024)
   PUBMED   39379383
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Kolonay,D.W., Sattler,K.M., Strawser,C., Rafael-Fortney,J.,
            Mihaylova,M.M., Miller,K.E., Lepper,C. and Baskin,K.K.
  TITLE     Temporal regulation of the Mediator complex during muscle
            proliferation, differentiation, regeneration, aging, and disease
  JOURNAL   Front Cell Dev Biol 12, 1331563 (2024)
   PUBMED   38690566
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3413)
  AUTHORS   Tan,C., Zhu,S., Chen,Z., Liu,C., Li,Y.E., Zhu,M., Zhang,Z.,
            Zhang,Z., Zhang,L., Gu,Y., Liang,Z., Boyer,T.G., Ouyang,K.,
            Evans,S.M. and Fang,X.
  TITLE     Mediator complex proximal Tail subunit MED30 is critical for
            Mediator core stability and cardiomyocyte transcriptional network
  JOURNAL   PLoS Genet 17 (9), e1009785 (2021)
   PUBMED   34506481
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3413)
  AUTHORS   Kagey,M.H., Newman,J.J., Bilodeau,S., Zhan,Y., Orlando,D.A., van
            Berkum,N.L., Ebmeier,C.C., Goossens,J., Rahl,P.B., Levine,S.S.,
            Taatjes,D.J., Dekker,J. and Young,R.A.
  TITLE     Mediator and cohesin connect gene expression and chromatin
            architecture
  JOURNAL   Nature 467 (7314), 430-435 (2010)
   PUBMED   20720539
  REMARK    Erratum:[Nature. 2011 Apr 14;472(7342):247]
REFERENCE   5  (bases 1 to 3413)
  AUTHORS   Krebs,A.R., Demmers,J., Karmodiya,K., Chang,N.C., Chang,A.C. and
            Tora,L.
  TITLE     ATAC and Mediator coactivators form a stable complex and regulate a
            set of non-coding RNA genes
  JOURNAL   EMBO Rep 11 (7), 541-547 (2010)
   PUBMED   20508642
REFERENCE   6  (bases 1 to 3413)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Kitamura,H., Nakagawa,T., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of FLJ
            genes: the complete nucleotide sequences of 110 mouse
            FLJ-homologous cDnas identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (2), 127-135 (2004)
   PUBMED   15449545
REFERENCE   7  (bases 1 to 3413)
  AUTHORS   Maynard,T.M., Haskell,G.T., Peters,A.Z., Sikich,L., Lieberman,J.A.
            and LaMantia,A.S.
  TITLE     A comprehensive analysis of 22q11 gene expression in the developing
            and adult brain
  JOURNAL   Proc Natl Acad Sci U S A 100 (24), 14433-14438 (2003)
   PUBMED   14614146
REFERENCE   8  (bases 1 to 3413)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 3413)
  AUTHORS   Mittler,G., Kremmer,E., Timmers,H.T. and Meisterernst,M.
  TITLE     Novel critical role of a human Mediator complex for basal RNA
            polymerase II transcription
  JOURNAL   EMBO Rep 2 (9), 808-813 (2001)
   PUBMED   11559591
REFERENCE   10 (bases 1 to 3413)
  AUTHORS   Berti,L., Mittler,G., Przemeck,G.K., Stelzer,G., Gunzler,B.,
            Amati,F., Conti,E., Dallapiccola,B., Hrabe de Angelis,M.,
            Novelli,G. and Meisterernst,M.
  TITLE     Isolation and characterization of a novel gene from the DiGeorge
            chromosomal region that encodes for a mediator subunit
  JOURNAL   Genomics 74 (3), 320-332 (2001)
   PUBMED   11414760
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC087802.13, AK142224.1 and AC115733.14.
            
            On Oct 26, 2013 this sequence version replaced NM_033609.2.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK142224.1, AF328770.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                AC087802.13        104579-104613       c
            36-567              AK142224.1         2-533
            568-679             AK142224.1         535-646
            680-760             AC115733.14        184863-184943       c
            761-2767            AK142224.1         728-2734
            2768-3413           AC115733.14        161597-162242       c
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 10.94 cM"
     gene            1..3413
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /note="mediator complex subunit 15"
                     /db_xref="GeneID:94112"
                     /db_xref="MGI:MGI:2137379"
     exon            1..192
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /inference="alignment:Splign:2.1.0"
     exon            193..280
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /inference="alignment:Splign:2.1.0"
     exon            281..332
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /inference="alignment:Splign:2.1.0"
     exon            333..362
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /inference="alignment:Splign:2.1.0"
     exon            363..575
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /inference="alignment:Splign:2.1.0"
     exon            576..829
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /inference="alignment:Splign:2.1.0"
     exon            830..1159
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1288
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..1408
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /inference="alignment:Splign:2.1.0"
     exon            1409..1536
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /inference="alignment:Splign:2.1.0"
     exon            1537..1664
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1799
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..1863
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /inference="alignment:Splign:2.1.0"
     exon            1864..1930
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /inference="alignment:Splign:2.1.0"
     exon            1931..2091
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /inference="alignment:Splign:2.1.0"
     exon            2092..2258
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /inference="alignment:Splign:2.1.0"
     exon            2259..2357
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /inference="alignment:Splign:2.1.0"
     exon            2358..3413
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3380..3385
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /note="hexamer: AATAAA"
     polyA_site      3410
                     /gene="Med15"
                     /gene_synonym="A230074L19Rik; mPcqap; Pcqap"
                     /note="major polyA site"
ORIGIN      
        1 acttccggcg tggcccagta gcgggatcgc tcggcttccg ggttcgggcc tgtcgctgtg
       61 gtagaggcgg cggcgccggg ggtggccggt agggactcgg gctacaagag taggggctcc
      121 gggcatggac gtttcggggc aggagaccga ctggcgtagc gccgcctttc ggcagaagct
      181 ggtcagccaa attgaggatg ccatgaggaa agctggtgtg gcccacagta aatctagcaa
      241 ggatatggag agtcatgtgt tcctgaaggc caagacccgg gatgagtatc tttcccttgt
      301 ggcccgactc attatccatt tccgagatat tcataacaag aaatcccaag cttctgtcag
      361 tgaccccatg aatgcactgc agagccttac tggtggaccc accccaggag cagctgggat
      421 tggcatgcct cctcggggcc caggacagtc cctgggtggg atgggtggcc ttggcgctat
      481 gggacaacca ctgcccctct ccgggcaacc accccctgga acctctggaa tggcccctca
      541 tggcatggct gtggtgtcta cagcaactcc acagactcag ctgcagctcc agcaagtggc
      601 attgcagcaa cagcagcagc agcaacaaca acagcaattc cagcaacagc aggcagcact
      661 gcagcaacag cagcaacagc agcagcagca acagcagcaa cagcagttcc aggcacaaca
      721 gaatgccatg cagcaacagt tccaagcagt agtgcagcag cagcagcttc agcagcagca
      781 gcagcagcag cacctgatta agttgcatca tcaaagccag caacaacaga tacaacagca
      841 gcaactgcag aggatggcac agttgcagct gcagcaacag caacagcagc aacagcagca
      901 ggctttgcag gcccagccac caatgcagca gccatcaatg cagcagccac agcctccccc
      961 ttctcaggcc ctaccccagc agctgtcaca gctgcatcat ccacagcatc accagccacc
     1021 acctcaggct cagcagtccc ccattgctca aaaccaacca ccacagatcc caccacagtc
     1081 acagagccag cctttggtgt cacaagcaca agcccttcct ggaccgatgc tgtatgctgc
     1141 ccaacagcag ctgaaatttg tccgtgctcc gatggtggtc cagcagccgc aagtgcagcc
     1201 ccaggtgcag caggtgcagc cccaggtgca gccgcaggca gcagtgcagg cagcacagtc
     1261 tgcccagatg gtagctcccg gcgtccagat gattgctgaa gccttggccc aaggcgggat
     1321 gcacgtaaga gcccggttcc cgcccacctc caccatgtct gctggcccgt caagctccat
     1381 ctctttgggc ggacagccca caacacaggt cagccaaagc agcctcacca tgctgtcctc
     1441 accgtcacca ggccagcagg tgcagacccc acagtcgatg ccacctcccc cacagccgtc
     1501 cccacaacct ggctcacagc ccaactctaa tgtcagctcc ggccctgccc catctcccag
     1561 cagcttcctg cctagccctt caccacagcc ttctcagagc ccagtgacag cacgcacccc
     1621 acagaacttc agcgttcctt cccctggacc tttaaacacc cctgtgaacc ccagctctgt
     1681 catgagccca gctggctcta gccaggctga ggagcagcag tacctggaca agctgaagca
     1741 attgtccaag tacatcgagc ccctgcgacg catgatcaac aagatcgaca agaatgaaga
     1801 cagaaaaaag gacttaagta agatgaagag cctgctggac atcctcaccg acccctcgaa
     1861 gaggtgtccc ctgaagaccc tgcaaaagtg tgagattgcg ctggagaagc tcaagaatga
     1921 catggcagtg cccacacccc caccaccccc agttcttcca accaaacagc aggacctgtg
     1981 ccaaccactc ctagatgcag tcctggccaa catccgttca cctgtcttca accattccct
     2041 gtaccgcaca tttgtgccag ccatgatggc catccatggc ccacctatcg tgtcgccagt
     2101 ggtgtgttcc cggaagcgcc ggtttgagga ggatgagcgg cagagcatac ccaatgtgct
     2161 gcaaggtgaa gtggcaaggc tggatcccaa gttcctggtg aacttggacc cttctcactg
     2221 cagcaacaac ggtactgtcc acctgatctg caagctggat gataaggacc tccctagtgt
     2281 gccaccactg gagctcagtg tgcctgctga ctaccctgcc cagagcccaa tgtggatcga
     2341 ccgtcagtgg caatatgatg ccaacccctt tctgcagtca gtgcaccggt gcatgacctc
     2401 aaggctgctg cagctccctg acaagcactc ggtcacagcg ctgctcaaca cctgggccca
     2461 gagcatccac caggcctgcc tctcagctgc ctagcaaact ggaacttcag gactgccagc
     2521 agcccttctg gctaaagatc tcacgccgcc taccagacat tactaagtgt tggcttctta
     2581 gagggcctgg gttaggttag ctttcctgct tttaccttct ttggtgggga cctgccagct
     2641 gtttcccatt cttgtgcagt attgactggg cagctgtgaa gctggctgct tgagggcctg
     2701 gcggtttccc cagaaggttt tccattggtt tggtttgttt cgttattttt tgttttttgt
     2761 ttttgcagag gtcaggcccc acacttcctc cagcctttct gggcttccat gcccactcag
     2821 cagcatgaga actcagggcc ccatcaaagc atctctgttt tgcaatacat tgtactcatt
     2881 atgtggagaa tgtgcaggac ataggaaacc ctcactctat acacagttct ccagtcacac
     2941 tggggctcag gcgtgcctcc taaggcatgt ggacacgtca caggaacacg tctgaatcca
     3001 gggcacacat ttcagtttgg aagaaagacc aatagggcat ccgcaggatc tctgccacca
     3061 gcagaacctc cactttctgg gcctcctcag tcatgtgagg aggagaatca gctggaggca
     3121 aggggctgtc cctgacattc ctgcgccaat cttgggtgaa gggctgtcag tgctatgata
     3181 cacaggcaga taggcagata ctatcactgg gacccaccag cctcaacacc atattctctt
     3241 attctctata ccaccaccaa gcgtgtgggt ttccttcgga atactgcaga tggcgctcgc
     3301 cacactgggc agcaatgggg agcactctgg tgaaaattcc cctttttata tgtgcactac
     3361 tctgtcatat gattcttata ataaatttat tattcctgtg tttgtcagga gtt
//
