LOCUS       NM_001080931           10540 bp    mRNA    linear   ROD 18-JUN-2024
DEFINITION  Mus musculus mediator complex subunit 13 (Med13), mRNA.
ACCESSION   NM_001080931 XM_109726
VERSION     NM_001080931.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10540)
  AUTHORS   Kolonay,D.W., Sattler,K.M., Strawser,C., Rafael-Fortney,J.,
            Mihaylova,M.M., Miller,K.E., Lepper,C. and Baskin,K.K.
  TITLE     Temporal regulation of the Mediator complex during muscle
            proliferation, differentiation, regeneration, aging, and disease
  JOURNAL   Front Cell Dev Biol 12, 1331563 (2024)
   PUBMED   38690566
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10540)
  AUTHORS   Ren,M., Yang,Y., Heng,K.H.Y., Ng,L.Y., Chong,C.Y., Ng,Y.T.,
            Gorur-Shandilya,S., Lee,R.M.Q., Lim,K.L., Zhang,J. and Koh,T.W.
  TITLE     MED13 and glycolysis are conserved modifiers of
            alpha-synuclein-associated neurodegeneration
  JOURNAL   Cell Rep 41 (12), 111852 (2022)
   PUBMED   36543134
  REMARK    GeneRIF: MED13 and glycolysis are conserved modifiers of
            alpha-synuclein-associated neurodegeneration.
REFERENCE   3  (bases 1 to 10540)
  AUTHORS   Coulis,G., Londhe,A.D., Sagabala,R.S., Shi,Y., Labbe,D.P.,
            Bergeron,A., Sahadevan,P., Nawaito,S.A., Sahmi,F., Josse,M.,
            Vinette,V., Guertin,M.C., Karsenty,G., Tremblay,M.L., Tardif,J.C.,
            Allen,B.G. and Boivin,B.
  TITLE     Protein tyrosine phosphatase 1B regulates miR-208b-argonaute 2
            association and thyroid hormone responsiveness in cardiac
            hypertrophy
  JOURNAL   Sci Signal 15 (730), eabn6875 (2022)
   PUBMED   35439023
  REMARK    GeneRIF: Protein tyrosine phosphatase 1B regulates
            miR-208b-argonaute 2 association and thyroid hormone responsiveness
            in cardiac hypertrophy.
REFERENCE   4  (bases 1 to 10540)
  AUTHORS   Tan,C., Zhu,S., Chen,Z., Liu,C., Li,Y.E., Zhu,M., Zhang,Z.,
            Zhang,Z., Zhang,L., Gu,Y., Liang,Z., Boyer,T.G., Ouyang,K.,
            Evans,S.M. and Fang,X.
  TITLE     Mediator complex proximal Tail subunit MED30 is critical for
            Mediator core stability and cardiomyocyte transcriptional network
  JOURNAL   PLoS Genet 17 (9), e1009785 (2021)
   PUBMED   34506481
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10540)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 10540)
  AUTHORS   Kile,B.T., Hentges,K.E., Clark,A.T., Nakamura,H., Salinger,A.P.,
            Liu,B., Box,N., Stockton,D.W., Johnson,R.L., Behringer,R.R.,
            Bradley,A. and Justice,M.J.
  TITLE     Functional genetic analysis of mouse chromosome 11
  JOURNAL   Nature 425 (6953), 81-86 (2003)
   PUBMED   12955145
REFERENCE   7  (bases 1 to 10540)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   8  (bases 1 to 10540)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 10540)
  AUTHORS   Ito,M., Okano,H.J., Darnell,R.B. and Roeder,R.G.
  TITLE     The TRAP100 component of the TRAP/Mediator complex is essential in
            broad transcriptional events and development
  JOURNAL   EMBO J 21 (13), 3464-3475 (2002)
   PUBMED   12093747
REFERENCE   10 (bases 1 to 10540)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL596256.14 and AL592065.7.
            
            On Feb 15, 2019 this sequence version replaced NM_001080931.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223971.11999.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-137               AL596256.14        44425-44561         c
            138-372             AL596256.14        42239-42473         c
            373-541             AL596256.14        32825-32993         c
            542-687             AL596256.14        18848-18993         c
            688-885             AL596256.14        17953-18150         c
            886-1080            AL596256.14        16133-16327         c
            1081-1243           AL596256.14        15412-15574         c
            1244-1354           AL596256.14        14767-14877         c
            1355-2038           AL596256.14        6313-6996           c
            2039-2252           AL592065.7         6124-6337
            2253-2331           AL592065.7         7974-8052
            2332-2453           AL592065.7         11381-11502
            2454-2544           AL592065.7         13481-13571
            2545-2759           AL592065.7         13692-13906
            2760-2956           AL592065.7         14190-14386
            2957-3873           AL592065.7         15840-16756
            3874-4035           AL592065.7         23832-23993
            4036-4259           AL592065.7         25880-26103
            4260-4449           AL592065.7         26756-26945
            4450-4906           AL592065.7         28146-28602
            4907-5126           AL592065.7         29704-29923
            5127-5318           AL592065.7         31094-31285
            5319-5542           AL592065.7         31613-31836
            5543-5685           AL592065.7         35301-35443
            5686-5844           AL592065.7         36114-36272
            5845-6030           AL592065.7         36381-36566
            6031-6179           AL592065.7         38068-38216
            6180-6353           AL592065.7         39489-39662
            6354-6454           AL592065.7         42473-42573
            6455-10540          AL592065.7         43918-48003
FEATURES             Location/Qualifiers
     source          1..10540
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 51.75 cM"
     gene            1..10540
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /note="mediator complex subunit 13"
                     /db_xref="GeneID:327987"
                     /db_xref="MGI:MGI:3029632"
     exon            1..137
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..6587
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /note="thyroid hormone receptor associated protein, 240
                     kDa subunit; thyroid hormone receptor associated protein
                     1; mediator of RNA polymerase II transcription, subunit
                     13; thyroid hormone receptor-associated protein complex
                     240 kDa component; Zmediator complex subunit 13"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 13"
                     /protein_id="NP_001074400.1"
                     /db_xref="CCDS:CCDS36267.1"
                     /db_xref="GeneID:327987"
                     /db_xref="MGI:MGI:3029632"
                     /translation="MSSSFVSNGASLEDCHCNLFCLADLTGIKWKRYVWQGPTSAPIL
                     FPVTEEDPILSSFSRCLKADVLGVWRRDQRPGRRELWIFWWGKDPNFADLIHHDLSEE
                     EDGVWENGLSYECRTLLFKAVHNLLERCLMNRNFVRIGKWFVKPYEKDEKPINKSEHL
                     SCSFTFFLHGDSNVCTSVEINQHQPVYLLSEEHVTLAQQSNSPFQVILSPFGLNGTLT
                     GQAFKMSDSATKKLIGEWKQFYPISCGLKEMSEEKQDDMDWEDDSLAAVEVLVAGVRM
                     IYPACFVLVPQSDIPAPSSVGASHCSASCLGIHQVPASTRDPAMSSVTLTPPTSPEEV
                     QTVDPQSAQKWVKFSSVSDGFSTDSTSHHGGKIPRKLANHVVDRVWQECNMNRLQNKR
                     KYSATSSGLCEEETADKIGCWDFVEATQRTSCSCLRHKSLKTRNTGQQGQAPSLGQQQ
                     QVLPKHKTNEKQDKSEKPQKRPLTPFHHRVSVSDEIGMDTDSASQRLVISAADSQVRF
                     SNIRTNDVAKTPQMHGTELANSPQPPPLSPHPCDVVDEGVTKTPSTPQSQHFYQMPTP
                     DPLVPTKPMEDRIDSLSQSFPPPFQEAVEPTVYVGTAVSLEEDEANVAWKYYKVPKKK
                     DVEFLPPQLPNDKFKDDPVGPFGQESVTSVTELMVQCKKPLKVSDEIVQQYQIKNQYL
                     SAIASDTEQEPKIDPYAFVEGDEEFIFTDKKDRQNSEREAGKKHKVEDGTSAVTVLSH
                     EEDAMSLFSPSKQDAPRPTNHARPPSTSLIYDSDLAVSYTDLDNLFNSDEDELTPGSK
                     KSASGSDDKASSKESKTGNLDPLSCISTADLHKMYPTPPSLEQHIMGFSPMNMNNKEY
                     GSVDTAPGGTVLEGNSSSVGTQFRIEVEEGFCSPKPSEIKDFSYVYKPENCQVLVGCS
                     MFAPLKTLPSHCLPPIKLPEECVYRQSWTVGKLDLLPSGPSMPFIKEGDGSNLDQDYG
                     PAYTPQTHASFGMPPSSAPPSNGGAGILPSPSTPRFPTPRTPRTPRTPRGAGGPASAQ
                     GSVKYENSDLYSPASTPSTCRPLNSVEPATVPSIPEAHSLYVNLILSESVMNLFKDCN
                     FDSCCICVCNMNIKGADVGVYIPDPTQEAQYRCTCGFSAVMNRKFGNNSGLFLEDELD
                     IIGRNTDCGKEAEKRFEALRASSVENVNGGLKESEKVPDELILLLQDQCTNLFSPFGA
                     ADQDPFPKVGISSNWVRVEERDCCSDCCLALEHGRQFMDNMSGGKVDEALVRSSCLHP
                     WAKQNDASVQCSQDILRMLLSLQPVLQDAIQKKRTVRPWGVQGPLTWQQFHKMAGRGS
                     YGTDESPEPLPIPTFLLGYDYDFLVLSPFALPYWEKLMLEPYGSQRDIAYVVLCPENE
                     ALLNGARSFFRDLTAIYESCRLGQHRPISRLLTDGIMKVGATASKKLSEKFVTEWFSQ
                     AADGNNEAFSKLKLYAQVCRYDLGPYLASQPLDSSLLSQPNLVAPPNQSLVTAPQMTN
                     TGNANAPSATLASAASSTMTMTSGVPISTSVATANSTLTTTSSSSSSSLSSGVSSNKL
                     PSFPPFGSMNTSGTGSMSAQASTVQSGQLGGQQSSSLQAAGISGESASLPTQPHPDVS
                     ESTMDRDKVGIPTDGDSHAITYPPAIVVYIIDPFTYENKDESTNSSNVWTLGLLRCFL
                     EMVQTLPPHIKSTVSVQIVPCQYLLQPVKHDDRQIYSQHLKSLAFSVFTQCRRPLPTS
                     TNVKTLTGFGPGLAMETALKSPDRPECIRLYTPPFILAPVKDKQTELGETFGEAGQKY
                     NVLFVGYCLSHDQRWILASCTDLYGELLETCIINIDVPNRARRKKGSARRFGLQKLWE
                     WCLGLVQMSSLPWRVVIGRLGRIGHGELKDWSCLLSRRNLQSLSKRLKDMCRMCGISA
                     ADSPSILSACLVAMEPQGSFVIMPDSVSTGSVFGRSTTLNMQTPQLNTPQDTSCTHIL
                     VFPTSASVQVASATYTTENLDLAFNPNNDGADGMGIFDLLDTGDDLDPDIINILPASP
                     TASPVHSPGSHYPHGGDAGKGQGTDRLLSTESHDEVTNILQQPLALGYFVSTAKAGPL
                     PDWFWSACPQAQYQCPLFLKASLHLHVPSVQSDELLHSKHSHPLDSNQTSDVLRFVLE
                     QYNALSWLTCDPAVQDRRSCLPVHFVVLNQLYNFIMNML"
     exon            138..372
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            373..541
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            542..687
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            688..885
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            886..1080
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1243
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            1244..1354
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..2038
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            2039..2252
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            2253..2331
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            2332..2453
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            2454..2544
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            2545..2759
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            2760..2956
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            2957..3873
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            3874..4035
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            4036..4259
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            4260..4449
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            4450..4906
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            4907..5126
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            5127..5318
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            5319..5542
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            5543..5685
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            5686..5844
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            5845..6030
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            6031..6179
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            6180..6353
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            6354..6454
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     exon            6455..10540
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7141..7146
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /note="hexamer: ATTAAA"
     polyA_site      7162
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
     regulatory      7445..7450
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /note="hexamer: AATAAA"
     polyA_site      7468
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
     regulatory      10518..10523
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /note="hexamer: AATAAA"
     polyA_site      10540
                     /gene="Med13"
                     /gene_synonym="1110067M05Rik; D030023K18; Thrap1; Trap240"
                     /note="major polyA site"
ORIGIN      
        1 ctctctggtc ggaggcggcg gtaatggcgg atggtgggtt gtggcgccgg cggcggctgc
       61 tgtgagggac gatgagttcc tccttcgtgt cgaacggggc cagcctggaa gattgtcact
      121 gtaacctctt ctgcctggcc gacttgacag gaattaagtg gaaaagatat gtatggcaag
      181 gcccaacttc cgcccctatt ctgtttccgg tgacagaaga agaccccatt ttgagcagtt
      241 ttagtcgatg ccttaaggca gatgtacttg gtgtttggcg gcgagatcaa agacctggaa
      301 gaagagaatt gtggatattt tggtggggta aagatcccaa ttttgctgac cttattcacc
      361 atgacttatc agaagaggaa gatggagtat gggagaatgg cctttcctat gaatgccgta
      421 ctttgctttt caaagcagtt cacaatctgt tggagcgatg tttgatgaac cgaaactttg
      481 tacgaattgg caagtggttt gtgaagccat atgagaaaga tgaaaaacct ataaataaaa
      541 gtgaacactt gtcctgctct ttcacctttt tcttgcatgg agacagcaat gtttgtacca
      601 gtgtggaaat taaccaacat caacctgtat accttctcag tgaagagcat gtcacccttg
      661 ctcaacagtc taatagccca tttcaagtta tcttaagtcc atttggacta aatggcactc
      721 tcacgggaca ggctttcaag atgtctgatt cagctacgaa aaaattaatt ggtgaatgga
      781 aacagttcta tcctatttca tgtggtttga aggagatgtc tgaggagaaa caggacgata
      841 tggattggga agacgattct ttggctgcgg tggaagttct tgttgcgggt gtccgaatga
      901 tctacccagc atgctttgtg ctagtccctc agtcagacat tcctgctcct agctctgtgg
      961 gagcttctca ctgttcagct tcttgcttgg gtatccatca agtgcctgct tccacaagag
     1021 atcctgctat gtcttcagtt actctcactc cacctacatc tcctgaggaa gtccaaacag
     1081 ttgatcctca gtccgcacag aagtgggtca aattttcttc agtatccgat ggcttcagca
     1141 ctgacagtac tagccatcat ggtgggaaaa tacccagaaa attagcaaat catgtggtgg
     1201 atagagtctg gcaagaatgt aatatgaaca gattacagaa caagaggaag tattctgcta
     1261 catccagcgg cctgtgtgag gaagagactg ccgataaaat aggatgctgg gactttgttg
     1321 aagccacaca aagaacaagc tgcagttgtt tgaggcataa aagtctcaag acaagaaata
     1381 ctgggcaaca aggacaggca ccatctttag ggcagcaaca gcaagtactt cctaagcaca
     1441 agaccaatga gaagcaagac aagagtgaga agccacagaa gcgccccttg actccctttc
     1501 accatcgtgt atcagttagt gatgagattg gcatggacac agattcagcc agccaaagac
     1561 ttgtgatctc tgctgcagat agtcaagtga ggttttcaaa catccgaact aatgatgtag
     1621 caaagactcc tcagatgcat ggcactgaac tggcaaattc acctcagcct cccccactta
     1681 gtcctcatcc ttgtgatgtg gttgatgaag gagtgactaa aacaccttca actccgcaga
     1741 gtcaacattt ttatcaaatg ccaacaccgg atcccttggt tcctaccaaa ccaatggaag
     1801 ataggataga cagtttgtcc cagtctttcc cacctccatt tcaggaagct gtagaaccta
     1861 ccgtgtatgt tggtacagca gtaagcttgg aagaagatga agctaatgta gcctggaagt
     1921 attacaaagt cccaaagaaa aaagatgtag aatttttacc acctcaactt ccaaatgata
     1981 aattcaagga tgatccagtt ggaccttttg gacaggaaag tgtaacatca gttacagagt
     2041 taatggtgca gtgtaagaag cctttaaaag tttctgatga aatagtccag caatatcaaa
     2101 ttaaaaatca gtatctttca gcaatagcat ctgatacaga acaagaacct aaaattgatc
     2161 catatgcatt tgttgaagga gatgaggaat tcatttttac tgataaaaaa gatagacaaa
     2221 atagcgagag agaagctggg aagaaacaca aggtggaaga tgggacatct gctgtcacag
     2281 tgttatcaca cgaagaagat gctatgtcat tatttagtcc ctctaaacaa gatgctccac
     2341 gccctacaaa tcatgcccgt cctccatcaa ccagtttgat ttatgactca gatttggctg
     2401 tgtcttacac tgaccttgat aacctcttca attctgatga agatgaatta acacctggat
     2461 ctaaaaagtc agcaagtgga tcagatgata aagccagctc caaagaatca aagacaggaa
     2521 atctggatcc gttgtcctgc ataagcactg cagacctcca caaaatgtac cccacgccac
     2581 cctcactgga acagcacatt atgggcttct ctccaatgaa tatgaataac aaagaatacg
     2641 gtagtgtgga cacagcaccc ggagggactg tcctggaagg aaatagctct agtgtaggaa
     2701 cgcagttcag aatcgaggtc gaggaaggat tctgtagccc taaaccgtct gaaattaaag
     2761 atttttctta tgtctataag cccgaaaatt gtcaagttct agtaggatgt tccatgtttg
     2821 cacctttaaa aactctacca agccattgtc tgcctcctat caaattgcca gaagagtgtg
     2881 tttaccggca gagctggact gttggaaaat tggacttgct tccttcaggc ccttcaatgc
     2941 cattcatcaa ggagggtgat ggcagtaatt tggatcagga ctatggcccc gcctacacac
     3001 cgcaaaccca tgcttctttt gggatgcctc cgagcagcgc acctcctagt aatggtggtg
     3061 caggaattct tccttcccca tccaccccta ggtttccaac tccaaggact ccaaggactc
     3121 caaggacccc tcgtggggct ggtgggcctg ctagtgctca aggttcagtc aagtatgaga
     3181 attcagactt gtattcacca gcttccaccc catctacatg ccgacccctc aattctgttg
     3241 aacctgcaac tgttccttcc atccctgaag cgcacagtct ttatgtaaac ctcatccttt
     3301 cagaatcagt aatgaatttg tttaaagact gtaattttga tagttgctgt atttgtgtct
     3361 gcaatatgaa tatcaagggt gccgatgttg gagtttacat tccagaccca acccaagaag
     3421 cacagtatcg gtgcacctgt ggcttcagtg ctgtcatgaa ccgaaaattt ggaaataatt
     3481 caggattatt tcttgaagat gaacttgaca tcataggacg taacacagac tgtgggaaag
     3541 aagcagaaaa acgttttgaa gctctcaggg cttcctctgt ggagaatgtt aatggaggcc
     3601 tgaaggaatc tgaaaaggtg cctgatgaat taatattact gctacaagat caatgcacta
     3661 atttattttc accctttgga gcagcagacc aagatccttt tcccaaagtg ggtataagta
     3721 gcaattgggt gcgtgttgag gagcgggact gttgtagtga ctgctgcctc gccttagaac
     3781 atgggcgtca gttcatggat aatatgtcag gagggaaagt tgatgaagca cttgtcagaa
     3841 gctcatgctt acatccgtgg gccaagcaaa atgatgcgag cgtgcagtgt tcacaggata
     3901 tacttcggat gctcctttct cttcagccag ttcttcaaga tgctattcag aaaaaaagaa
     3961 cagtgaggcc ttggggtgtt caaggtcctc tcacatggca acaatttcat aaaatggctg
     4021 gccgaggctc ttatggaact gatgaatctc cggaaccact gccgattccc acgtttttgt
     4081 tgggttatga ttacgatttt ctggtgcttt ctccatttgc tctcccttac tgggagaaac
     4141 ttatgctgga accctatgga tctcaaagag atatagccta tgttgtactc tgtccagaga
     4201 atgaagcttt gttaaatgga gccagaagct tttttagaga tctcactgca atatatgagt
     4261 cctgtcgatt aggccaacac agacctattt ctcgattgtt gacggatggg atcatgaaag
     4321 tgggagctac tgcatcaaag aaactttcag aaaagtttgt aacagaatgg ttttctcagg
     4381 cagctgatgg taacaatgaa gcattttcca aattgaagct gtatgcacaa gtctgcagat
     4441 atgatttagg tccatatctt gcttctcagc cgttggacag ctctctgctc tcccagccaa
     4501 atttagttgc ccctccaaat cagtctttgg ttactgcacc tcagatgaca aatactggaa
     4561 acgctaatgc tccatctgcc accttagcat ctgcggcaag tagcactatg acaatgactt
     4621 caggtgttcc catatctact tcagttgcca cagctaattc aactttgacc acaacttcat
     4681 cttcatcatc ttccagtctg agtagtggag tatcatcaaa taaactgcct tcgtttccac
     4741 cctttggcag tatgaacact agtggtacag gatccatgtc tgcacaagca agtacggttc
     4801 agagtggcca gctaggagga cagcagtcgt catctctgca ggcagcagga atatcagggg
     4861 agtcagcttc tcttcccact cagccacatc ctgatgtgtc tgaaagcaca atggatcgtg
     4921 ataaagtggg gattcccaca gatggtgatt cacatgcaat tacctaccct cctgcaattg
     4981 ttgtttatat aatcgatcct tttacatatg aaaataaaga cgagagcact aactcttcta
     5041 atgtatggac tttggggctc cttcgatgct tcttggaaat ggtccaaact cttcctcctc
     5101 atatcaagag cactgtttct gtgcagattg ttccttgtca gtacctgtta caacctgtga
     5161 aacacgacga cagacagatc tactcccagc atttaaaatc tttggctttt tcggtgttta
     5221 cccagtgtcg gagaccactt ccaacatcaa ccaatgtaaa aacactgact gggtttggtc
     5281 caggcttagc tatggaaact gctcttaaaa gtcctgatag accagagtgt attcgacttt
     5341 atacaccacc ttttattctg gctccggtga aggacaaaca aacagagcta ggagagacct
     5401 ttggagaagc tggacagaaa tacaatgttc tttttgtggg ctactgttta tcacatgatc
     5461 aaaggtggat tcttgcatct tgcacagatc tatatggaga actcctagaa acatgtatca
     5521 ttaacatcga tgttccaaat agggcccgtc ggaaaaaggg ttctgctaga agatttggtc
     5581 tacagaaact gtgggagtgg tgcttgggac ttgttcagat gagctcttta ccatggagag
     5641 ttgtgattgg tcgcctggga aggattggac atggagagtt gaaagattgg agttgtttgc
     5701 tgagtcgtcg aaacttgcaa tctctaagca aaaggctcaa agacatgtgt aggatgtgtg
     5761 gcatatctgc tgcagactct ccaagtatcc ttagtgcttg tttggttgca atggaacccc
     5821 aaggttcttt tgttatcatg ccagattctg tgtcaactgg ttctgttttt ggaagaagta
     5881 ccactctaaa tatgcagacg cctcagctaa ataccccaca agatacatcc tgtactcata
     5941 tacttgtgtt ccctacttct gcttctgtgc aagtagcttc agctacttac accactgaaa
     6001 atttggattt agctttcaat cccaacaatg atggagcaga cggaatgggt atctttgatt
     6061 tattagacac aggagatgat cttgatcctg atatcattaa tatccttcct gcatctccaa
     6121 ctgcatctcc tgtacattct ccaggctctc attaccccca tggaggtgat gctggcaagg
     6181 gtcagggtac tgatcggcta ctttcaacag aatcgcatga tgaagtaact aatattcttc
     6241 aacaaccatt ggcccttgga tactttgtat caactgccaa agcgggtcca ttacctgact
     6301 ggttctggtc agcatgccct caagcacaat atcaatgccc cctttttctt aaggcctctt
     6361 tgcacctcca cgtgccttca gtgcaatctg acgagctgct tcacagcaaa cactcccacc
     6421 cactggactc aaatcagact tcagatgtcc tcaggtttgt tttggaacag tacaatgcac
     6481 tctcatggct aacctgtgac cctgcagtcc aggacagacg ctcatgtctc ccagttcatt
     6541 ttgtggtgct gaatcagtta tataacttca tcatgaatat gctgtgatcc tcgtttgatg
     6601 gaattacgca aggaaagaac gggaagaaag gatattttgc tgcaagatta agttacagtt
     6661 ctcagtggaa gtcatttgtg atggggtcta attcttatta cttcaacaaa tattgttttg
     6721 acttgggggg cggggctata accctatttt tcattgactt tactgagctc tttaggatga
     6781 tgactgatta ttcaaaacgt attataacac tttcgtagca aaaattaacc ttttttttcc
     6841 agtcacagta tttgtgaaaa gtaatgagcc atagtaccca ttaatgttaa agaatattaa
     6901 agcatggaga ggaacatgag gaacaatgaa ttccaacata tggctttaga acatcaggat
     6961 gttcttgtat tggatatagt atctagtatt caaaaatgcc tgcatctctt atttattgta
     7021 agtttttaaa tgtataaatt gtcttatatt tcttaacctc ttttataaaa attttcctag
     7081 aaggtttata ctgccttctt gctttaaagc aattggtcta aaatatatgt aatcgtctta
     7141 attaaaacgt tgcagtaggg ttgcttttag agtattattt ttttgtaagg gggtggggtg
     7201 gacagtaaat ttgtattgtc tcaatgtaca gtttaacagg gatagagggg aaataatgtc
     7261 catgccattg tgtgtggaag atttacagct aagctgtagt tgcagagtac atgtacagta
     7321 atgaagttca ctgtgtttat aaactgaaaa ggtaccgggt cttacagcat tttatatatc
     7381 acacctttac agaataacat gatggcaata tacatacgtg atattgttag gtggaataac
     7441 ttagaataaa atgagaattc ttgagttata ttttgtacta tggtttaggg ctatgactaa
     7501 tatttcaggc catttccagt gaaagaaact tagttttaca agaaaacatt tgctactgaa
     7561 tgcttacact aatcttggtg tttaatgtta catgcttaaa ttcttttcag ttttcacagt
     7621 ggcacattta ggcatgggga tagtactaat tatgaaattt cctgcaggat ctgttctaag
     7681 gttgaaattt agcccagctc taggcatttt acaaattatt ttaagaacag tcactcttga
     7741 ttgttttact ttttattttt aaattaaaga ttgggaatgc gtgtgagagt atgcataagc
     7801 atggcaggaa gagagggggg agagagaaag agagagagag tgagaaagaa agagagagtg
     7861 agagtgtgtg tgtatatgtg tgtgtgtgtg caatcaaact gtggtgtaaa tagatcctca
     7921 gtgaattctg gtattcagac tctgttccac tagtgaatga accattttct aaacgtttcc
     7981 ctttgacttt tttatttatt gaattttctt ggtttggaga tggcagtccc aaacaccaga
     8041 gtctgtactt ttctatgaca cagctcaaat taaagtaggg cacatgccaa ggaggttctc
     8101 acctccctaa agaagggact tgaattttag ggactttaat tcaccctttc cttcaatcca
     8161 gctttccccc ttcttgtttg cacatgccaa gataactgct tttatgcagg ctttacccct
     8221 gaaaaactcc tttctacagt gctgctcacg agagcccagc cagtctcatg cctgcattgc
     8281 tgacttgaac tcacagtcgc caagtctacc tatctttctt ggaagcagtc tttcaaaatt
     8341 cctacttgta tattcatcca ttgttgacaa ggacaaaatc agttgtattt acaaaactct
     8401 taactcggta tttttttttt aaactgaatc ctgttttgta aataatatac atttaatgtt
     8461 gcttagcata aaaaatactt tcttacagta atcttcttta aattgggtat atgaaaacat
     8521 taccaactct tgtttttcta taaagaaata cagttaataa caaggtttca aattattcct
     8581 ttaacattaa tagaacaatc caaatgttga gcatattttg atattaaact aaagatcacg
     8641 tctctgtttt taaattatgc taacaccacc acagattatg ttgacgtatt ttcttctgta
     8701 ttttcttggt gggacttgag atttgaggaa aaaagatgct tatttggaat ttctattaag
     8761 aactatcctt gttattttct tagctcaata attactcaag aaaaataaaa gattataatc
     8821 tcagtttaaa attactaatg tgtgacaacc atacctcagt ttgaaataga tttaacaaga
     8881 ttattccctg acattttctt ccatgtagcc ataagcaggg acccagcata caattcttct
     8941 tacctaggaa tcaggaacta gtcccaaata tacaggattg agtcatggtt tgcttttttt
     9001 ttttttcctt tccttttttt ctccccttta cacatcagat tttgctcaaa taggaggtaa
     9061 ctaaatagcc attagaagaa gaagtgaagc ctttcatgca ggatgataga tctaacttat
     9121 caatgtactg tcacagtaca ttaatcgttt tatttgagta ggtaaacaaa tttgcacact
     9181 tttccccagt gaattcccaa caccttgact tcctcatttc agtgctaata acctgttttg
     9241 tgtgcagtta atccattctg aagcaggtga aaggtcagta ttttagcatt ttaaaagatg
     9301 agttatataa tgtctgcttc cagccagtct gcaacatcta gccatacctt tcttatgcaa
     9361 gccattcagt tatcaggact gtgaattaac actgtatgaa tacatttctg tacaccttat
     9421 tgtttggcca gagggccacc aagtgtactt atatgtaatc cttaaatttt aaactagctt
     9481 gtgattttta aatatttcta aacttttctt aaaccactaa aattaagctc ttactactta
     9541 gtcaactatc ctcagctgta ttcgtcttca attgtcagta tggcacagat cactgtatta
     9601 aaatgctctt tcgtcttcat atttaccttc tgaggtcatt ttttaactta atgtgttact
     9661 acaaagattt gcagatcttt aatcaagcac tatgttaata ctgtaatatc aaaatactat
     9721 gtcgaattat ttcagatgtt cagattgaat agggtaaaag aaagtcttta aatgctatcg
     9781 catcctagac cctcttccca cctactatgg cattgcatat tagtctgttg agacattcat
     9841 tgttgcttga ctgacatttt ggtctgggtt ttcttttaat attttatttt aattaaaatt
     9901 aggaaaaaat gtgttcaaca attttttttt tgtcccatct tctccaaatc agtgggaaaa
     9961 gcttaccttt tggtatatct tagtgttttc atgggcccag aactgtggtt taaattttta
    10021 tatgtattct cattctcata ttcactcata ctcactcttt ttctctctct ccctctctct
    10081 ccctctctcc cccccccttc tcttttgtgg agtataaatt aaaaaaactg gatttgggac
    10141 ctacaatact cctcaggttg agatattctt gaagttttaa aatagcttta gtttttaaag
    10201 taaactggat ttgtggacct taaagttact gagtttaaac tacaaattgt aacatcatta
    10261 ctgaacatgt ccgcatcaac cctctgagaa taacttggtc actttatgaa ggggcgtttt
    10321 taagttgttt agcagtgaca tttaatatgg tccaattgct tttcttttta acatgacaaa
    10381 caattttaaa agagactaag gaacaaacac tattgcgccg aatgccataa cactgagttg
    10441 tacaaattgt gattgaggag atgaaaaagt ttatactttt taaaaaaaaa aaaaacaaaa
    10501 aacaaaactt caaatggaat aaattattca tgaagccttc
//
