LOCUS       NM_021521               8464 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus mediator complex subunit 12 (Med12), mRNA.
ACCESSION   NM_021521
VERSION     NM_021521.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Kolonay,D.W., Sattler,K.M., Strawser,C., Rafael-Fortney,J.,
            Mihaylova,M.M., Miller,K.E., Lepper,C. and Baskin,K.K.
  TITLE     Temporal regulation of the Mediator complex during muscle
            proliferation, differentiation, regeneration, aging, and disease
  JOURNAL   Front Cell Dev Biol 12, 1331563 (2024)
   PUBMED   38690566
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8464)
  AUTHORS   Okada,M., Fujimoto,M., Srivastava,P., Pandey,A., Takii,R. and
            Nakai,A.
  TITLE     The Mediator subunit MED12 promotes formation of HSF1 condensates
            on heat shock response element arrays in heat-shocked cells
  JOURNAL   FEBS Lett 597 (13), 1702-1717 (2023)
   PUBMED   36971000
  REMARK    GeneRIF: The Mediator subunit MED12 promotes formation of HSF1
            condensates on heat shock response element arrays in heat-shocked
            cells.
REFERENCE   3  (bases 1 to 8464)
  AUTHORS   Venigalla,S., Straub,J., Idigo,O., Rinderle,C., Stephens,J.M. and
            Newman,J.J.
  TITLE     MED12 Regulates Human Adipose-Derived Stem Cell Adipogenesis and
            Mediator Kinase Subunit Expression in Murine Adipose Depots
  JOURNAL   Stem Cells Dev 31 (5-6), 119-131 (2022)
   PUBMED   35018809
  REMARK    GeneRIF: MED12 Regulates Human Adipose-Derived Stem Cell
            Adipogenesis and Mediator Kinase Subunit Expression in Murine
            Adipose Depots.
REFERENCE   4  (bases 1 to 8464)
  AUTHORS   Sooraj,D., Sun,C., Doan,A., Garama,D.J., Dannappel,M.V., Zhu,D.,
            Chua,H.K., Mahara,S., Wan Hassan,W.A., Tay,Y.K., Guanizo,A.,
            Croagh,D., Prodanovic,Z., Gough,D.J., Wan,C. and Firestein,R.
  TITLE     MED12 and BRD4 cooperate to sustain cancer growth upon loss of
            mediator kinase
  JOURNAL   Mol Cell 82 (1), 123-139 (2022)
   PUBMED   34910943
  REMARK    GeneRIF: MED12 and BRD4 cooperate to sustain cancer growth upon
            loss of mediator kinase.
REFERENCE   5  (bases 1 to 8464)
  AUTHORS   Tan,C., Zhu,S., Chen,Z., Liu,C., Li,Y.E., Zhu,M., Zhang,Z.,
            Zhang,Z., Zhang,L., Gu,Y., Liang,Z., Boyer,T.G., Ouyang,K.,
            Evans,S.M. and Fang,X.
  TITLE     Mediator complex proximal Tail subunit MED30 is critical for
            Mediator core stability and cardiomyocyte transcriptional network
  JOURNAL   PLoS Genet 17 (9), e1009785 (2021)
   PUBMED   34506481
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8464)
  AUTHORS   Malik,S., Guermah,M., Yuan,C.X., Wu,W., Yamamura,S. and Roeder,R.G.
  TITLE     Structural and functional organization of TRAP220, the
            TRAP/mediator subunit that is targeted by nuclear receptors
  JOURNAL   Mol Cell Biol 24 (18), 8244-8254 (2004)
   PUBMED   15340084
  REMARK    Erratum:[Mol Cell Biol. 2022 Nov 17;42(11):e0035922. doi:
            10.1128/mcb.00359-22.. PMID: 36255214]
REFERENCE   7  (bases 1 to 8464)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   8  (bases 1 to 8464)
  AUTHORS   Ge,K., Guermah,M., Yuan,C.X., Ito,M., Wallberg,A.E.,
            Spiegelman,B.M. and Roeder,R.G.
  TITLE     Transcription coactivator TRAP220 is required for PPAR gamma
            2-stimulated adipogenesis
  JOURNAL   Nature 417 (6888), 563-567 (2002)
   PUBMED   12037571
REFERENCE   9  (bases 1 to 8464)
  AUTHORS   Philibert,R.A., King,B.H., Winfield,S., Cook,E.H., Lee,Y.H.,
            Stubblefield,B., Damschroder-Williams,P., Dea,C., Palotie,A.,
            Tengstrom,C., Martin,B.M. and Ginns,E.I.
  TITLE     Association of an X-chromosome dodecamer insertional variant allele
            with mental retardation
  JOURNAL   Mol Psychiatry 3 (4), 303-309 (1998)
   PUBMED   9702738
  REMARK    Erratum:[Mol Psychiatry 1999 Mar;4(2):197]
REFERENCE   10 (bases 1 to 8464)
  AUTHORS   Duboule,D., Haenlin,M., Galliot,B. and Mohier,E.
  TITLE     DNA sequences homologous to the Drosophila opa repeat are present
            in murine mRNAs that are differentially expressed in fetuses and
            adult tissues
  JOURNAL   Mol Cell Biol 7 (5), 2003-2006 (1987)
   PUBMED   2885744
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC090654.1, BC057119.1 and BC110696.1.
            
            On Feb 10, 2007 this sequence version replaced NM_021521.1.
            
            Sequence Note: This sequence has been modified as follows: removed
            16 bp suspected to be vector contamination from the 5' end.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC090654.1, SRR10223971.18769.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164138
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-269               BC090654.1         1-269
            270-6893            BC057119.1         4-6627
            6894-8464           BC110696.1         2248-3818
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 43.91 cM"
     gene            1..8464
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /note="mediator complex subunit 12"
                     /db_xref="GeneID:59024"
                     /db_xref="MGI:MGI:1926212"
     exon            1..269
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     CDS             171..6743
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /note="trinucleotide repeat containing 11 (THR-associated
                     protein); OPA-containing protein 1; trinucleotide
                     repeat-containing gene 11 protein; thyroid hormone
                     receptor-associated protein complex 230 kDa component;
                     trinucleotide repeat containing 11 (THR-associated
                     protein, 230 kDa subunit); mediator of RNA polymerase II
                     transcription, subunit 12 homolog"
                     /codon_start=1
                     /product="mediator of RNA polymerase II transcription
                     subunit 12"
                     /protein_id="NP_067496.2"
                     /db_xref="CCDS:CCDS41078.1"
                     /db_xref="GeneID:59024"
                     /db_xref="MGI:MGI:1926212"
                     /translation="MAAFGILSYEHRPLKRLRLGPPDVYPQDPKQKEDELTALNVKQG
                     FNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLSDTGRRKSLMNQKDNF
                     WLVTARSQSAINTWFTDLAGTKPLTHLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLI
                     KMTCAYYAAMSETKVKKKNTADPFTEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGS
                     TIGPLPHDVEMAIRQWDYNEKLALFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKL
                     LLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVIAAQSTSSLPTTPA
                     PQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTG
                     SPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATA
                     GFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLL
                     CEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSVASGSLSAPSAPI
                     FQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGD
                     LAFGAPGPRPPSPFDDPTDDPERKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEK
                     PDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPAKEKIEGTLGILYDQPRHVQYATHFP
                     IPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIV
                     PLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQ
                     ITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSD
                     LVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCI
                     LAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENP
                     AAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKS
                     LSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLED
                     LIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSC
                     DRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVPGGTEELPEEEGGGGSSGR
                     RQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQR
                     LMQLICYPHRLLDNEDGENPQRQRIKRILKNLDQWTMRQSSLELQLMIKQTPNTEMNS
                     LLENIAKATIEVFQQSAETGSSSGSTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKL
                     PTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQD
                     EQREGLLASLHSQVHQIVINWRENQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRST
                     QQTTEWAQLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEEN
                     KRAYMNLVKKLQKDLGERQSDSLEKVHQLLPLPKQNRDVITCEPQGSLIDTKGNKIAG
                     FDSIFKKEGLQVSTKQKISPWELFEGLKPSTAPLSWAWFGTVRVDRRVARGEEQQRLL
                     LYHTHLRPRPRAYYLEPLPLPPEDEEPPAPALLEPEKKAPEPPKTDKPGAAPPSTEER
                     KKKSTKGKKRSQPATKNEDYGMGPGRSGPYGVTVPPDLLHHANPGSISHLSYRQSSMG
                     LYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPTYPGVLPTTMSTVMGLEPSSYKTSVY
                     RQQQPTVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTSYVSHVGL
                     QQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLT
                     STQRFSHQTLQQTPMMGTMTPLSAQGVQAGVRSTSILPEQQQQQQQQQQQQQQQQQQQ
                     QQQQQQQQQQQQQQYHIRQQQQQQQMLRQQQQQQQQQQQQQQQQQQQQQQQQQQQPHQ
                     QQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQPSTNIFGRY
                     "
     exon            270..374
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            375..566
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            567..726
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            727..908
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            909..1019
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..1274
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            1275..1421
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1521
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            1522..1658
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            1659..1790
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            1791..1917
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            1918..2147
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            2148..2228
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            2229..2399
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            2400..2544
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            2545..2595
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            2596..2714
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            2715..2858
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            2859..3022
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            3023..3154
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            3155..3382
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            3383..3527
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            3528..3648
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            3649..3750
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            3751..3864
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            3865..4040
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            4041..4220
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            4221..4292
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            4293..4426
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            4427..4588
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            4589..4700
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            4701..4790
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            4791..4900
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            4901..5036
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            5037..5198
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            5199..5576
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            5577..5727
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            5728..5933
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            5934..6011
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            6012..6229
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            6230..6476
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            6477..6617
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            6618..6699
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     exon            6700..8447
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6862..6867
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /note="hexamer: AATGAA"
     polyA_site      6893
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /note="major polyA site"
     regulatory      6952..6957
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /note="hexamer: AATACA"
     polyA_site      6975
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /note="major polyA site"
     regulatory      8415..8420
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
                     /note="hexamer: GATAAA"
     polyA_site      8444
                     /gene="Med12"
                     /gene_synonym="230kDa; Mopa; OPA-1; Tnrc11; Trap230"
ORIGIN      
        1 agagtttcta acgtgccccg cctccgccac cccccttctc tcggcctcgg tcctctctac
       61 cgccgcccgg ctcgctgtcc ccgtgctccc tcccgtcctc ggccctccgc cccttccctc
      121 cccacctcgc ctggccctgc aggcgcctca ggcggtacgg gctgggcaag atggcggctt
      181 tcgggatctt gagctatgaa caccgacccc tgaagcggct gcggctgggg cctcccgatg
      241 tgtaccctca agatcccaaa cagaaggagg atgaactgac ggctttgaat gtaaaacaag
      301 gtttcaataa ccagcctgct gtctctgggg acgaacatgg cagtgccaag aacgtcaact
      361 tcaatcctgc caagatcagt tccaacttca gcagcatcat cgcagagaag ttaaggtgta
      421 atactctctc tgacactggt cgcaggaagt ccctaatgaa ccagaaggac aacttctggc
      481 tggtgactgc aagatctcag agtgctatta acacctggtt cactgacctg gctggcacca
      541 aaccactcac acacctagcc aaaaaggtcc ccattttcag taaaaaggaa gaagtatttg
      601 ggtatttggc caaatacacg gtgcctgtga tgcgggctgc ctggctcatt aagatgactt
      661 gtgcctacta tgcagcaatg tctgagacta aggttaagaa gaaaaatact gctgacccct
      721 tcactgaatg gactcaaatc atcacaaagt acttgtggga gcagctacag aagatggctg
      781 agtactatcg gccagggcct gcaggaagtg gaggctgtgg ttctactata gggcctttgc
      841 cccatgatgt agagatggcc atcaggcagt gggattacaa tgagaagctc gccttgttca
      901 tgtttcagga tggaatgctg gacagacatg agttcctgac ttgggtgctt gagtgttttg
      961 agaaaatacg ccctggagaa gatgaattgc ttaaattgct gcttccccta ctgctgcgat
     1021 actcagggga gtttgttcag tctgcctatc tgtcccgccg ccttgcctac ttctgtaccc
     1081 ggagattggc tctccagctg gatggtgtga gcagccactc atctcatgtc atagctgctc
     1141 agtcaacaag ttctctgccc actaccccag cacctcagcc cccaactagc agtacaccct
     1201 cgactccctt tagtgacctg cttatgtgcc ctcagcatcg tcccctggtt tttggcctca
     1261 gctgtatcct tcagaccatc cttctgtgtt gccctagtgc cctagtttgg cactactcat
     1321 tgactgatag ccgaattaag accggctcac cacttgacca cctgcctatt gctccttcca
     1381 acttgcccat gccagagggt aacagtgcct ttactcagca ggtccgtgca aaattacgag
     1441 agatcgaaca acagatcaag gagcgtggac aagcagttga ggttcgctgg tcttttgata
     1501 agtgccagga agctactgca ggtttcacca ttggacgggt gctccatact ttagaagtgc
     1561 tggatagcca tagtttcgag cgctctgact ttagtaactc tcttgactcc ctttgtaatc
     1621 gaatctttgg attggggcct agtaaggatg gtcatgagat ttcctctgat gatgatgctg
     1681 tggtatcatt attgtgtgaa tgggctgtaa gctgcaaacg ctcaggtcgg catcgtgcta
     1741 tggtagtagc caaactccta gagaagagac aagcagaaat tgaggctgag cgctgcggag
     1801 aatctgaagc agctgatgag aagggctccg tcgcctctgg ttccctttcc gctcctagtg
     1861 cacccatttt ccaggatgtc ctcctgcagt ttctggatac acaggctccc atgctgactg
     1921 atccccgaag tgaaagtgag cgagtagaat tctttaactt ggtactgctc ttctgtgaac
     1981 tgatccgaca tgatgtcttc tcccataata tgtacacctg cactctcatc tctcgggggg
     2041 atcttgcatt tggagcccct ggtcctcggc ctccctctcc ctttgatgat cctacagatg
     2101 atccagagcg caaggaggct gaaggcagca gcagcagcaa gctagaggac ccagggctct
     2161 ctgaatctat ggacatcgac cctagttcca gtgtgctttt tgaggacatg gaaaagcctg
     2221 atttctcatt gttctcccct actatgcctt gtgaggggaa gggaagccca tcccctgaga
     2281 aaccagatgt cgaaaaggaa gtaaaacccc cagccaaaga gaagatcgag gggacacttg
     2341 ggattctgta tgaccagcca cgacatgtgc agtatgccac acactttcca atcccacagg
     2401 aggagtcatg cagccatgag tgcaaccagc ggttggtcgt actgtttggg gtggggaagc
     2461 agcgagatga tgcccgccat gccatcaaga agattaccaa ggatatcctg aaggttctga
     2521 atcgcaaggg gacagcagaa actgaccagc ttgctcctat tgtgcctctg aatcctggag
     2581 acctgacatt cttaggtggg gaagatgggc agaagcgacg acgcaaccgg cccgaagcct
     2641 tccccactgc tgaagatatt tttgctaagt tccagcacct ttcccattat gaccaacacc
     2701 aggtcacggc tcaggtctcc cggaacgttc tggagcagat cacgagcttc gcccttggca
     2761 tgtcgtacca cttgcctctg gtgcagcatg tgcagttcat cttcgacctc atggaatatt
     2821 cactgagcat cagtggcctc atcgactttg ccatccagtt actgaatgag ctgagtgtgg
     2881 ttgaggcgga gctccttctc aaatcgtcgg atctggtggg cagctacact accagcctgt
     2941 gcttatgtat cgtggctgtc cttcgacact atcatgcctg cctcatcctc aatcaggacc
     3001 agatggcgca agtgtttgag gggctctgtg gcgtggtgaa acatggaatg aaccgttcag
     3061 atggctcctc tgcagagcgc tgtatccttg cttatctcta tgatctgtat acctcctgta
     3121 gccatttaaa gagcaaattt ggggagctct tcagtgactt ttgctcaaaa gtgaagaaca
     3181 ccatctactg taacgtggag ccatcggaat ccaatatgcg ctgggcaccg gagttcatga
     3241 ttgacactct ggagaaccct gccgctcaca ctttcaccta cacggggctc ggcaagagtc
     3301 ttagtgagaa ccctgctaac cgctatagct ttgtgtgcaa tgctcttatg cacgtctgcg
     3361 tgggccacca tgatcctgat agggtaaatg acatcgccat cctttgtgca gagctgaccg
     3421 gctattgcaa gtccttgagt gctgagtggc taggagtact taaggccttg tgctgctcct
     3481 cgaacaatgg cacttgtggt ttcaatgatc tcctgtgcaa cgtagacgtc agtgatttgt
     3541 cttttcatga ttccttggct acttttgttg ctatcctcat cgcccgtcaa tgtttgctcc
     3601 tagaagacct gattcgctgt gcagccatcc cttcactcct caatgctgct tgtagtgagc
     3661 aggattctga gccaggagcc aggcttactt gccgaatcct cctccacctg ttcaagacgc
     3721 cacaactcaa tccttgccag tctgatggaa acaaacctac tgttggaatc cggtcctcct
     3781 gtgaccgcca cctgctggct gcctcccaga accgcatcgt ggatggggct gtgtttgctg
     3841 ttctcaaggc tgtgtttgta ctcggagatg cggagctaaa aggttcaggc tttactgtgc
     3901 cgggaggaac agaagaactt ccagaagagg agggaggagg tggtagtagc ggtcggagac
     3961 agggtggccg caacatctct gtggagacag caagtctgga tgtctatgcc aagtacgtgc
     4021 tgcgaagcat ctgccaacag gaatgggtag gagaacgttg ccttaagtca ctgtgtgagg
     4081 atagcaatga tctacaagac ccagtgttga gtagtgccca ggcccagcgc ctcatgcaac
     4141 ttatctgcta cccacatcga ctgctggaca atgaggatgg agaaaacccc cagcggcagc
     4201 gcattaaacg tattctcaag aatttagacc aatggaccat gcgccagtcc tctttggagc
     4261 tacagttgat gatcaagcag acccccaaca ctgagatgaa ctctctcttg gagaacattg
     4321 ccaaggccac aatcgaggtt ttccaacagt cagcagagac ggggtcatct tctggaagta
     4381 cagcaagcaa catgcccagc agcagcaaga ccaaacctgt gctcagctct ctagagcgat
     4441 ctggtgtatg gttggtggct cctctcattg ccaaactgcc cacttcagtc cagggccatg
     4501 tattaaaagc tgctggggag gaactggaaa agggtcagca cctgggctct tcttcccgta
     4561 aagaacgaga tcgacagaaa cagaagagca tgtccctgtt gagccaacag cctttcttat
     4621 cactggtgct aacatgtctg aaaggacagg atgagcagcg cgagggactc ctggcctccc
     4681 tccacagcca ggtgcaccag attgtgatta attggcgaga aaaccagtac ttagatgatt
     4741 gcaaaccaaa gcagctaatg catgaggcac tcaaactgcg gctcaacctg gtggggggca
     4801 tgtttgacac tgtgcagcgt agtacccagc agactacaga gtgggcccag cttctccttg
     4861 agatcatcat cagcggcact gtggacatgc agtctaacaa tgagctcttc actactgtgt
     4921 tggacatgct gagcgtgctt atcaacggaa cgttggctgc agacatgtcc agtatctcgc
     4981 aaggcagcat ggaggaaaac aagcgtgcat atatgaacct ggtgaagaag cttcagaagg
     5041 acttggggga gcgccaatca gacagtctgg agaaggttca ccaactgttg ccactaccca
     5101 agcagaaccg agatgtcata acctgtgagc cacagggctc ccttattgac accaagggca
     5161 acaagattgc tggcttcgat tccatcttca agaaggaggg tctacaagtt tctaccaaac
     5221 aaaagatctc tccctgggag ctttttgagg gcctgaagcc atcaacagca ccactgtcat
     5281 gggcctggtt tggcacagtc cgagtggacc gcagagtggc acgaggggag gagcagcagc
     5341 ggctgttgct ctatcatacc cacctgaggc ctcgacccag agcctattac ctggaaccac
     5401 tacctctgcc cccagaagat gaggagccac cagcccctgc cctactagag cctgagaaaa
     5461 aggctcctga gccccccaag actgacaaac caggggctgc tcctccgagc actgaggagc
     5521 gcaaaaagaa gtctaccaag ggcaaaaaac gcagccagcc agccaccaag aacgaggact
     5581 atggcatggg gccaggtcgg agtggcccct atggtgtgac agtgcctcca gaccttctac
     5641 accatgcaaa tcctggttct atatcccacc ttagctacag gcaaagctcc atgggcctgt
     5701 atacccaaaa ccagccacta cctgctggtg gccctcgtgt ggatccatac cgccccgtgc
     5761 gattaccaat gcaaaagctg ccaactcgac caacttatcc cggtgtgctg cctacaacta
     5821 tgtctactgt catgggccta gaaccctctt cttataagac atctgtatac cggcagcagc
     5881 aacccacagt gccccaggga cagcgccttc gccaacagct ccaggcaaag atacagagtc
     5941 aggggatgtt gggacagtca tctgtccatc agatgacccc tagttcttcc tatggtttgc
     6001 agacttccca gggctataca tcctatgttt ctcatgtggg attgcagcaa cacacaggcc
     6061 ctgcaggtac catggtgccc cccagctact ccagccagcc ttatcagagc acccaccctt
     6121 ctaccaatcc tactcttgta gatcctaccc gccacctgca acagcggccc agtggctatg
     6181 tgcatcagca ggccccaacc tatgggcatg gactgacttc cactcaaagg ttttcacacc
     6241 agacactgca gcagacaccc atgatgggta ccatgactcc gttgagtgcc cagggtgtcc
     6301 aggcaggcgt ccgttcaact tccatcctgc ctgagcagca gcaacaacaa cagcagcagc
     6361 agcaacagca gcagcagcaa cagcagcagc agcaacaaca acagcagcag cagcagcagc
     6421 aacaacaaca gcagtaccat atccgacagc aacagcagca gcagcagatg ctacggcaac
     6481 agcagcaaca acagcaacag cagcagcagc agcaacagca gcagcagcaa caacaacagc
     6541 aacagcagca gcagcagcca caccagcagc agcagcaggc agctcctccc caaccccagc
     6601 cccagtccca gccccagttc cagcgccagg gcctgcagca gacccagcag cagcaacaga
     6661 cagcagcttt ggtccggcaa cttcaacaac agctctctaa tacccagcca cagcccagca
     6721 ccaacatatt tggacgctac tgagtcacct ggaggaactg cttgtacact ggatgtggcc
     6781 cagcctttct gcttaattcc cagtcccctt cctgggcgag ctccagtagt ggttggggtc
     6841 cttccctcag gctccatttt taatgaattt ttagtatttt tgttaatgtg aaacattgaa
     6901 ctgttgggtt ttgtatatta tttatataga gatcctagag ctgctgcact caatacacag
     6961 agcttctttg caaaagaagt gtgtgaatgt acacatggaa gggtctcctg tgagagtacc
     7021 ggggaaagga tgaatcacta agaagcttca tgccttctgc atcgtctccc tcctcaccct
     7081 ctgcctttgc atttctgagt tctcgctgca ctgctacact tggttccctg ctcgaaagca
     7141 acccgaatga gctccaagcc cttggtcctc aactcaacag tgcagcttca tgacagcctt
     7201 tttcccactg gggatgtgcc actagagggg gggctcattg ggctggagag aaaccagtgc
     7261 aaccccatca tccttccaca acactccaga tgagagtagg gtagagatgc cagacaatgc
     7321 ctgtcattgt gtcgtctcat gtagtcccca caaatacacc aagctaacta gagaacttct
     7381 ctgaagggaa catttctaaa tagttgctgt ctgtgcgcat gagatggcat gtgatgtgac
     7441 agaagttccg actagttcta gcaggaagag agacaagcag gatgccacac ctgtttctcc
     7501 ctgacctgct ggaaccccat gttttcatac aacccacctt ggcagtttcc cagagtgact
     7561 gtttctcact ctcaacccaa aatttccttc agagtcactt ttgaaacaag ggctgggctg
     7621 gtctcaccat ctctgtggtc tgtttgcctt aggccctcaa tccaaggctg cgctgggggc
     7681 cccagctact ttatgacaat ttctgctcag ctaggactag aagaagtcta cattagtctg
     7741 gtggttgggt gataactgga gggtaccccc aggttgggtg ataactggag tataccccca
     7801 atctataatt acaccctatc actgggaggt gtcagttatg cccttaaaag cttgagtgcc
     7861 aactgatgaa tatccaagtc atttttaaaa atatctctga gcccacaaag cccatctaca
     7921 tacaggactt cctcaagtcg tgagaaagga acggggctcc caggtatggt aaagccagca
     7981 caactgaagc agtttttttc ttgacttgct ctatcctgga agcagcccaa ggggtagaag
     8041 tttggctagg ataagggacg ttcagcaggg atttaatggt gtatgtgtgc taggtcaagt
     8101 ggataggtgt attacatata gaaactagat aaaaacatta gctattgggg tgggggtaga
     8161 aaccagaatg gggcgagaac tggtcttttg agactgaatc caatccacct tgacagaagc
     8221 agcagaagtg aatgacattg acctctggga ggtaacagga tgagggaaaa gtgaccccac
     8281 tggtgattgc accttgggtg gtgctggtgc taaggagagg tgatgcttgc caaatgaggg
     8341 ctttgtggca agaaactgga tcttctccat acagtgcctc agtttctccc tgtatcaagg
     8401 tctcaagctt tgccgataaa gtttggattt gttttcctgg aaaatcaaaa aaaaaaaaaa
     8461 aaaa
//
