LOCUS       NM_010788              10576 bp    mRNA    linear   ROD 10-DEC-2024
DEFINITION  Mus musculus methyl CpG binding protein 2 (Mecp2), transcript
            variant 2, mRNA.
ACCESSION   NM_010788
VERSION     NM_010788.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   Kalani,L., Kim,B.H., de Chavez,A.R., Roemer,A., Mikhailov,A.,
            Merritt,J.K., Good,K.V., Chow,R.L., Delaney,K.R., Hendzel,M.J.,
            Zhou,Z., Neul,J.L., Vincent,J.B. and Ausio,J.
  TITLE     Testing the PEST hypothesis using relevant Rett mutations in MeCP2
            E1 and E2 isoforms
  JOURNAL   Hum Mol Genet 33 (21), 1833-1845 (2024)
   PUBMED   39137370
REFERENCE   2  (bases 1 to 10576)
  AUTHORS   Esposito,A., Seri,T., Breccia,M., Indrigo,M., De Rocco,G.,
            Nuzzolillo,F., Denti,V., Pappacena,F., Tartaglione,G., Serrao,S.,
            Paglia,G., Murru,L., de Pretis,S., Cioni,J.M., Landsberger,N.,
            Guarnieri,F.C. and Palmieri,M.
  TITLE     Unraveling autophagic imbalances and therapeutic insights in
            Mecp2-deficient models
  JOURNAL   EMBO Mol Med 16 (11), 2795-2826 (2024)
   PUBMED   39402139
REFERENCE   3  (bases 1 to 10576)
  AUTHORS   Chen,L., Zhang,M., Yang,X., Wang,Y., Huang,T., Li,X., Ban,Y.,
            Li,Q., Yang,Q., Zhang,Y., Zheng,Y., Wang,D., Wang,X., Shi,X.,
            Zhang,M., Sun,Y. and Wu,J.
  TITLE     Methyl-CpG-binding 2 K271 lactylation-mediated M2 macrophage
            polarization inhibits atherosclerosis
  JOURNAL   Theranostics 14 (11), 4256-4277 (2024)
   PUBMED   39113793
  REMARK    GeneRIF: Methyl-CpG-binding 2 K271 lactylation-mediated M2
            macrophage polarization inhibits atherosclerosis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10576)
  AUTHORS   Feng,Y., Wang,J., Liu,J., Zhou,Y., Jiang,Y., Zhou,W., Wu,F., Liu,X.
            and Luo,L.
  TITLE     Mecp2 Deficiency in Peripheral Sensory Neuron Improves Cognitive
            Function by Enhancing Hippocampal Dendritic Spine Densities in Mice
  JOURNAL   Cells 13 (11), 988 (2024)
   PUBMED   38891120
  REMARK    GeneRIF: Mecp2 Deficiency in Peripheral Sensory Neuron Improves
            Cognitive Function by Enhancing Hippocampal Dendritic Spine
            Densities in Mice.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10576)
  AUTHORS   Medeiros,D., Ayala-Baylon,K., Egido-Betancourt,H., Miller,E.,
            Chapleau,C., Robinson,H., Phillips,M.L., Yang,T., Longo,F.M., Li,W.
            and Pozzo-Miller,L.
  TITLE     A small-molecule TrkB ligand improves dendritic spine phenotypes
            and atypical behaviors in female Rett syndrome mice
  JOURNAL   Dis Model Mech 17 (6) (2024)
   PUBMED   38785269
REFERENCE   6  (bases 1 to 10576)
  AUTHORS   Kriaucionis,S. and Bird,A.
  TITLE     The major form of MeCP2 has a novel N-terminus generated by
            alternative splicing
  JOURNAL   Nucleic Acids Res 32 (5), 1818-1823 (2004)
   PUBMED   15034150
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 10576)
  AUTHORS   Tate,P., Skarnes,W. and Bird,A.
  TITLE     The methyl-CpG binding protein MeCP2 is essential for embryonic
            development in the mouse
  JOURNAL   Nat Genet 12 (2), 205-208 (1996)
   PUBMED   8563762
REFERENCE   8  (bases 1 to 10576)
  AUTHORS   Quaderi,N.A., Meehan,R.R., Tate,P.H., Cross,S.H., Bird,A.P.,
            Chatterjee,A., Herman,G.E. and Brown,S.D.
  TITLE     Genetic and physical mapping of a gene encoding a methyl CpG
            binding protein, Mecp2, to the mouse X chromosome
  JOURNAL   Genomics 22 (3), 648-651 (1994)
   PUBMED   8001979
REFERENCE   9  (bases 1 to 10576)
  AUTHORS   Lakso,M., Sauer,B., Mosinger,B. Jr., Lee,E.J., Manning,R.W.,
            Yu,S.H., Mulder,K.L. and Westphal,H.
  TITLE     Targeted oncogene activation by site-specific recombination in
            transgenic mice
  JOURNAL   Proc Natl Acad Sci U S A 89 (14), 6232-6236 (1992)
   PUBMED   1631115
REFERENCE   10 (bases 1 to 10576)
  AUTHORS   Klemsz,M.J., McKercher,S.R., Celada,A., Van Beveren,C. and
            Maki,R.A.
  TITLE     The macrophage and B cell-specific transcription factor PU.1 is
            related to the ets oncogene
  JOURNAL   Cell 61 (1), 113-124 (1990)
   PUBMED   2180582
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL672002.14 and BU700810.1.
            
            On Jun 27, 2014 this sequence version replaced NM_010788.3.
            
            Transcript Variant: This variant (2, also known as beta) has an
            additional exon in the 5' region, which results in the use of an
            alternate start codon, compared to variant 1. The encoded isoform
            (2) has a distinct N-terminus and is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF158181.1, BC027153.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            regulatory uORF        :: PMID: 15034150
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-181               AL672002.14        203851-204031       c
            182-305             AL672002.14        198250-198373       c
            306-656             AL672002.14        155323-155673       c
            657-10440           AL672002.14        145052-154835       c
            10441-10576         BU700810.1         1-136               c
FEATURES             Location/Qualifiers
     source          1..10576
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 37.63 cM"
     gene            1..10576
                     /gene="Mecp2"
                     /gene_synonym="1500041B07Rik; D630021H01Rik; Mbd5; WBP10"
                     /note="methyl CpG binding protein 2"
                     /db_xref="GeneID:17257"
                     /db_xref="MGI:MGI:99918"
     exon            1..181
                     /gene="Mecp2"
                     /gene_synonym="1500041B07Rik; D630021H01Rik; Mbd5; WBP10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      105..224
                     /regulatory_class="uORF"
                     /gene="Mecp2"
                     /gene_synonym="1500041B07Rik; D630021H01Rik; Mbd5; WBP10"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:15034150]"
                     /note="this uORF is predicted to encode a 39 aa peptide
                     (PMID:15034150)"
     exon            182..305
                     /gene="Mecp2"
                     /gene_synonym="1500041B07Rik; D630021H01Rik; Mbd5; WBP10"
                     /inference="alignment:Splign:2.1.0"
     CDS             280..1734
                     /gene="Mecp2"
                     /gene_synonym="1500041B07Rik; D630021H01Rik; Mbd5; WBP10"
                     /note="isoform 2 is encoded by transcript variant 2;
                     meCp-2 protein"
                     /codon_start=1
                     /product="methyl-CpG-binding protein 2 isoform 2"
                     /protein_id="NP_034918.1"
                     /db_xref="CCDS:CCDS41017.1"
                     /db_xref="GeneID:17257"
                     /db_xref="MGI:MGI:99918"
                     /translation="MVAGMLGLREEKSEDQDLQGLRDKPLKFKKAKKDKKEDKEGKHE
                     PLQPSAHHSAEPAEAGKAETSESSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPE
                     GWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVT
                     GRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTGRPKAAASEGVQVKRVLEKSPG
                     KLVVKMPFQASPGGKGEGGGATTSAQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVV
                     AAAAAEAKKKAVKESSIRSVHETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGK
                     GLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESTKAPMPLLPSPPPPEPE
                     SSEDPISPPEPQDLSSSICKEEKMPRGGSLESDGCPKEPAKTQPMVATTTTVAEKYKH
                     RGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS"
     exon            306..656
                     /gene="Mecp2"
                     /gene_synonym="1500041B07Rik; D630021H01Rik; Mbd5; WBP10"
                     /inference="alignment:Splign:2.1.0"
     exon            657..10559
                     /gene="Mecp2"
                     /gene_synonym="1500041B07Rik; D630021H01Rik; Mbd5; WBP10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10297..10302
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mecp2"
                     /gene_synonym="1500041B07Rik; D630021H01Rik; Mbd5; WBP10"
                     /note="hexamer: TATAAA"
     polyA_site      10322
                     /gene="Mecp2"
                     /gene_synonym="1500041B07Rik; D630021H01Rik; Mbd5; WBP10"
     regulatory      10537..10542
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mecp2"
                     /gene_synonym="1500041B07Rik; D630021H01Rik; Mbd5; WBP10"
                     /note="hexamer: GATAAA"
     polyA_site      10559
                     /gene="Mecp2"
                     /gene_synonym="1500041B07Rik; D630021H01Rik; Mbd5; WBP10"
                     /note="major polyA site"
ORIGIN      
        1 gtcgggaggg cggggcgcga cgtctgccgt gcggggtccc ggcatcggtt gcgcgcgcgc
       61 tccctcctct cggagagagg gctgtggtaa aacccgtccg gaaaatggcc gccgctgccg
      121 ccaccgccgc cgccgccgcc gcgccgagcg gaggaggagg aggaggcgag gaggagagac
      181 tgctccataa aaatacagac tcaccagttc ctgctttgat gtgacatgtg actccccaga
      241 atacaccttg cttctgtaga ccagctccaa caggattcca tggtagctgg gatgttaggg
      301 ctcagggagg aaaagtcaga agaccaggat ctccagggcc tcagagacaa gccactgaag
      361 tttaagaagg cgaagaaaga caagaaggag gacaaagaag gcaagcatga gccactacaa
      421 ccttcagccc accattctgc agagccagca gaggcaggca aagcagaaac atcagaaagc
      481 tcaggctctg ccccagcagt gccagaagcc tcggcttccc ccaaacagcg gcgctccatt
      541 atccgtgacc ggggacctat gtatgatgac cccaccttgc ctgaaggttg gacacgaaag
      601 cttaaacaaa ggaagtctgg ccgatctgct ggaaagtatg atgtatattt gatcaatccc
      661 cagggaaaag cttttcgctc taaagtagaa ttgattgcat actttgaaaa ggtgggagac
      721 acctccttgg accctaatga ttttgacttc acggtaactg ggagagggag cccctccagg
      781 agagagcaga aaccacctaa gaagcccaaa tctcccaaag ctccaggaac tggcaggggt
      841 cggggacgcc ccaaagggag cggcactggg agaccaaagg cagcagcatc agaaggtgtt
      901 caggtgaaaa gggtcctgga gaagagccct gggaaacttg ttgtcaagat gcctttccaa
      961 gcatcgcctg ggggtaaggg tgagggaggt ggggctacca catctgccca ggtcatggtg
     1021 atcaaacgcc ctggcagaaa gcgaaaagct gaagctgacc cccaggccat tcctaagaaa
     1081 cggggtagaa agcctgggag tgtggtggca gctgctgcag ctgaggccaa aaagaaagcc
     1141 gtgaaggagt cttccatacg gtctgtgcat gagactgtgc tccccatcaa gaagcgcaag
     1201 acccgggaga cggtcagcat cgaggtcaag gaagtggtga agcccctgct ggtgtccacc
     1261 cttggtgaga aaagcgggaa gggactgaag acctgcaaga gccctgggcg taaaagcaag
     1321 gagagcagcc ccaaggggcg cagcagcagt gcctcctccc cacctaagaa ggagcaccat
     1381 catcaccacc atcactcaga gtccacaaag gcccccatgc cactgctccc atccccaccc
     1441 ccacctgagc ctgagagctc tgaggacccc atcagccccc ctgagcctca ggacttgagc
     1501 agcagcatct gcaaagaaga gaagatgccc cgaggaggct cactggaaag cgatggctgc
     1561 cccaaggagc cagctaagac tcagcctatg gtcgccacca ctaccacagt tgcagaaaag
     1621 tacaaacacc gaggggaggg agagcgcaaa gacattgttt catcttccat gccaaggcca
     1681 aacagagagg agcctgtgga cagccggacg cccgtgaccg agagagttag ctgactttac
     1741 atagagcgga ttgcaaagca aaccaacaag aataaaggca gctgttgtct cttctcctta
     1801 tgggtagggc tctgacaaag cttcccgatt aactgaaata aaaaatattt ttttttcttt
     1861 cagtaaactt agagtttcgt ggcttcgggg tgggagtagt tggagcattg ggatgttttt
     1921 cttaccgaca agcacagtca ggttgaagac ctaaccaggg ccagaagtag ctttgcactt
     1981 ttctaaacta ggctccttca acaaggcttg ctgcagatac tactgaccag acaagctgtt
     2041 gaccaggcac tccccccaac aatatcctcc ctcttccccc cccccacccc cgccccgtgt
     2101 gctcgttagg gcaattgaga ggacactccc atttttggtg ccattgatgc cctgtccata
     2161 atagcttccc tgacttttac accaccccaa ctcccaatct gaaggactgg gaggtgtgat
     2221 gcaggagaaa ctatgggact cttgggagaa gactatggag ttggccagtg attaaggccc
     2281 agtaattcca actgtggtag cacagatctg gctccacatc aacccaatcc aaaactgaca
     2341 aggatatttt gcaaaaaaag aaagtggcac ctgtctgatc cagctctgac atggctagag
     2401 gtgagtccta aactgatggc ttataaacta gcctgagcca cagaagagta tggcccagag
     2461 tgaagtgtca tcatctgttc acaaggcatg ctcccctaga agataatgct aaagaggtgc
     2521 catggaggca gcaggacaaa gtacaggcag gctaggtgga gtcaagccag gcctagtgcc
     2581 acagaacaag agagcagtct gactagtaat taagagggaa gaaaggaaaa tattcttcca
     2641 attactttcc agttctcctt tagggacagc ttagaattat ttgcactatt gagtcttcat
     2701 gttcccactt caaaacaaac agatgctctg aaagcaaact ggcttgaaat ggtgacactg
     2761 tcccacaagc caccagacat ggcagtgttc agaactacct gtatctgtat atacctgcgc
     2821 ttgttttaaa gtgggctcag cacataggat tcccaagaag ctccgaaact ctaagtgttt
     2881 gctgcaattt tataaggact tcctgattgc tttctctctc gtccttccat ttcttccttc
     2941 cttccatttc atgctttcat ttcttcccct agcttctagt tgtttcttct gttccaggca
     3001 gctgcagtgc tgaaccacat ggttacctaa cagcagtcag ctgcagccct aggattcttc
     3061 ctgcccttta acttcccatt gccagtgcca ggtatcatat ttaaccttga gcaagagctg
     3121 ggctcttttg agccctccct aacctctgtg aagaagaaca agaaggtagg aagctcttgc
     3181 tcttgctaag aaaaatgtca aaaggctttc agaccttaaa caatgagcct tttcaccttt
     3241 tactctagaa aagtggacta gaaaatctgg gtcacattgg gtagctgaag gagatacaga
     3301 ggcccctatg gcctgccaga gtcgttgcat ggcccaacag gggctccatg cccactaccc
     3361 ttgaccctac tcagaaatct aatgtcatac ttagtgtggg caggggacct gtcaggacag
     3421 atgcagacct aagcagggag tgacaccagg gcccttggcc cttcttctga caaacataca
     3481 catcccaagt ctttttctag tggaattctt aacctcttgc tcactgggga ctgggaagca
     3541 tcagcacatc ccatatttca aactctgctc cataagtaca gtggtgaatt ttatagactt
     3601 gactttgctg tggggtttta attggtcagt tttaatttgg gatcccaaag ttttaacctc
     3661 cattcaggaa gtccttatct agctgcatat cttcatcata ttggtatatc cttttctgtg
     3721 tttacagaga tgtctcatat ctatcgaaat ctgtctgaga agtaccttat caaagtagca
     3781 aatgagacag cagtcttatg cttccagaaa cacccacagg cacgtcccat gtgagctgct
     3841 gccatgaact gtcgagtgtg tattgtcttg tgtattttcg ttaacgttcc ccagcttcct
     3901 tcctgcggtg taatcatgga agagtgaaac atcatagaaa tcgtctagca cttcctggcc
     3961 agtccttagt gatcaggaac cgtagttgac agttccaatt gatagcttaa gataaaacca
     4021 tgtttgtctc ttatggaatg gttagaacta agtgagagat cttgccccat tctgtttgcc
     4081 gaatcatagt tggactttta gtgtatttgt atccatttcc ttgtgctata aaagcaaacc
     4141 ctgcaaccag ctttctgtca ggcagtcctt ttgcctgctc tgcttttgat cctcttagtc
     4201 ttgcttctgg ttcctccctg gagagggagg aggggtcaga agaggaattc tggaggatcc
     4261 aggatatgtc cttctgaact cctgcttctt ccagtgacaa aaggccccta ctgccccacc
     4321 ccaacctgcc ccatgcactc ctctaggaca cctttccata cttttcacaa cacctagcca
     4381 ggttgacacc aagttgttta ttgtggtctg cttggaattt tacctgttag gcttacttag
     4441 tccaatcaaa tggactccaa gttgggtatc cctcatcttt ggaagacaac ctaggctgat
     4501 tagatattta cttttgggat tgcagcactt tgggtgccgt ttttctttta cttgggtttt
     4561 atctgcagct ccctcaccac caccaccacc ccccacttac ctgtatgtag aactgatttc
     4621 aaaactgcag gtggtggtaa ctgcagcttc ttagggtttt cttcacttct tgcttctttc
     4681 cccattccct catccacaaa taagggcatc acaagtcagt ctcctttaag caggcagctt
     4741 tggtggggtt tttcccctgg aagccaggga ccctgtcagg ctgcctctgc cttgtggtca
     4801 ggttgacagg aggttggagg gaaaagcctt aagtcatggg attctcacca gctgtgtctg
     4861 gctcagacct ggaatgtgac ctttattttg ttgtatttga acattgtaaa gtgtgggtgg
     4921 taccttaaac tgaatatgtg aagaatccag aaactgacca acagctttca gatacctggg
     4981 gctaggtcac taaggtcaca tccagtcttc cctaccctgt tctagttgtt agctactacc
     5041 tctcccagat agattgctgt atatcctcca actatgatca tcctggccca agcttgcctg
     5101 ttcttgagtc tgtcttaacc agtggaactg ctgcccttgg tgtgcagtga gttgaggact
     5161 cttggtcaca gccaggctct agtagtacag ctcctttctg ctggtgctgt atttccatat
     5221 caaaaggcac aggggagatc tagaaatgcc atctccccca gtccatcagt gccaaacaag
     5281 cccatgatcc cagcatgggt acagacaact ctgttcagtg ctatcacaac agactagagg
     5341 ccatgaacat tggacgtggg aaccagagca acccgaattg ctgctgcttt attcagcttt
     5401 ccgttgctct gacaatgata aaacaaggca gtaacttaaa acagactgcc aggtttggca
     5461 gagaaaggaa attccttagc tgacagcacc tctggatttt aaataggttg taataagtgg
     5521 ctcaaaccca tccaggaaaa agcaaaaggg ttagaactga ccagatgaga ccagcctgat
     5581 ttcatgcagc ccaaatggag tccagctgtc tgaactctgc agcacttctc tactacagtc
     5641 tcctagagca ttccagccag gctcttcagg ctgaggagac atcacaggtg ccagttcttc
     5701 aagaagactt ttgtgcatca gttcatagcc tatatctttg cccaagattg tagattcagg
     5761 ttaacactac agattctagg gcagatgact gagactcaga aaaaaagccc ctgtggactg
     5821 tggtatagcg aagtacaaaa actgaagggg gctagggcag atgccgcatg cctcatgcca
     5881 gagccaagcc ctctgctcca tccacatcct tttctggctc cttcttcctg ctctctgctt
     5941 cagtgaacca gccccactct gaagagattt gttgattctc tccattttta tgtctttctc
     6001 ttttaggtac tatatagaaa aggcttagtc taattgttat aaattgctag aatactgcct
     6061 cccccagggt ctaaaaatat atgctaaagg ggaaaacttg aacactgaaa ccagttctga
     6121 acaatttaga aggaaaacct tgaaaacatt taacaaaaaa ttatatttta atgtttatga
     6181 ataagaggag gcttttgaaa aaatgttgat ctataaatac ttactttagg cctgaggtgt
     6241 ctaatgagtg aactgagcaa tgggaactca aggctgaagc ctcctgcatc agaggaggta
     6301 gaaccaggag cctcttgaga tttgaggtgt tttagcattg gaaagccact ctttgggtag
     6361 ctggccccag aaactacttc tgaccttgtc atttggaatg gaggttagtg gtctgccaga
     6421 tgccaaagct gcatgagacc agctcttggt ttatcaattt gaacactcag taacctagaa
     6481 ggcccagcac aaagtgtctg ctctcttctt aactgagcct gccccagcac tactgcacaa
     6541 attagggagg gtctacttcc tacagagcat ccctccctgg gccccctccc atcctttgta
     6601 ctctacctac ctgaccttca ggatcttggc acatacgaaa tggctgtgta gcaagcactt
     6661 tggcatgccc tcctaaactt accccagagc ctctccctgc ctccttaagc cagtctgcct
     6721 gtcttctggg gaggtgttag agcccataga atggagagga gaaagaaaag aggaagaggc
     6781 aggcaggtag taaaaaggct ctgggaggaa agacagcctc ctaggctttg cacaagcagg
     6841 actcagcccc ttgtgggaac taagtgccat cttggagttt aagaacattt ggacaagttg
     6901 caaatgacct ttgctccttg ctcctctcac cttttatggg gccctgctta gcactgaaag
     6961 caaatgcgct gaaaaggcaa agaggtttgg ctcctgccca ctgatagtcc tttccctgca
     7021 gtgtttgtgt gtcaagtggc aaagctgttc ttcctggtga ctctgattag atccagtaac
     7081 ttaagagatt tgtatgcata ggtctgcttt gactcttcta ttctgggctt ttgatttgtt
     7141 tttcagtttt gcttttagtt ttcctatttt tattttatgc accaactaga cacacaaagc
     7201 agttgaattt atatatatat atatatatat atatctgtat atttcacaat tataaactca
     7261 ttttgcttgt gacgccacac acacacaaaa agaaaaacct tttaaaatta tacctgttgc
     7321 ttaattacaa tatttctgat aaccatagag taggacaagg gaaaaaattt aaaaagaaaa
     7381 aaaaaaaaag aaaaaacaca tctgtctgct ggtcacttct tcaatccaag cagatctgtg
     7441 atctttcctc gcgtctttca aagacttccc tgtgctaagt gaaggaagct ccaggctgca
     7501 cccaggtttt gtgctttgtt tctcctctgt tgtgaaaggg gccccaagat tctgggtaca
     7561 ggacagttca tttcagcatg gggtcaggag acaagagcac tccctttaca tgctgacgta
     7621 cagaacttag tgggaatagc ctagtcccca cctctaggga tggggagcta gcatgcatgg
     7681 gggtgaccca actccctcca cctttccctg gccaggaaga gcctgtgtac agtaagtctg
     7741 acaagctttc cccagttagc agggctcaga gcatttaaaa accctccaaa ctttgctgag
     7801 tctagggact agagagaaga tagaagattt ggtctatctc caaggtgtgt aagctgtacc
     7861 aggtagaatg ccagggaccc cagaaccaca tccaacagcc caatgggtct cctccagaaa
     7921 gtagtgaaga ctccagaaac atccctttct cttctccctg ctcccatgag taactgcatt
     7981 tgcttttgta atccttaatg agcattatct gctaaaaaaa aaaaattagc tgtaacagtt
     8041 ctttttgcaa aaggatcatt cttaaataat taaaaacacc cccccccaaa aaaaagtcca
     8101 gaaccttgtt cttccaaagc agagagcatt ataatcaggg ccaaaatctg tcccacacct
     8161 ctaccccatc tcctcatgat tgctgcttct aaggccagaa tacagcaaag atatttgtag
     8221 gccctttggg tgactgggct acccttggag ctcttggaag atgggctggg gaagcctctg
     8281 agaccctatc ctagggcctt gctctaggga gtaatcagta ttagtagagt gtcacaacat
     8341 tattccccag ccggcatgag atgggggcag aagaagccaa agggttgtct ccactgctac
     8401 ttacttggcc actgacaggt aggtgaccat gtatgtccat atgcatgttt tatggctgat
     8461 gtgagatcag cacccaagtt agcttcacct ggtgacctct aaccctgcct ggatggagca
     8521 ggccacctgg ttcaatgttt ctgggcagct ggacaatgga gtgcaaaagg cttacagaac
     8581 ttgaagcctt ttccttactt tgctagcacg gcctcctttt ccatttgatt tgtcactgct
     8641 tcagtcaata acagccgctc cagagtcagt agttgatgaa tatatgacca aatatcacca
     8701 ggactgttac tcaacgtgtg ccgagccctt tccttgtgct gggctccctg tgtacctgga
     8761 cactgtaatg tgtgctgtgt ttgctctcct tcctcttcct tccttgccct ttccttgtct
     8821 ttctggggtt tttctgttgg gtttggtttg gttttatttt tccttttgtg ttccaaacat
     8881 gaggttttct ctactggtcc tctttaactg tggtgttgag gcttctattt gtgtaatttt
     8941 tggtgggtga aaggaacttt gctaagtaaa tctcttctgt gtttgaaatg aagtctgtat
     9001 tgtaactatg tttaaagtaa ttgttccaga gacaaatgct tctaggtaca ttttcattac
     9061 aaacaaagca tttgaaggga gggaagtggt gaataagaca agaggggcaa tctgaattga
     9121 tccctgccca gatcagccag aagctaccaa aagttaagca ctggttttcc attccaagtc
     9181 aagagactga agctgatgtt ttgccatttt caaagtcaaa gcaaaaccag cttttccacc
     9241 caatggattc tttgcttctc cttcccagat tattactact gctgtaataa tctaggagtg
     9301 ccaggaggga aaggagtatt aacacagagc tgtgctcact gagtatggaa aggcttggtc
     9361 tgagttttca ggaggatgac ccactgtgga catggggaga agacagaaga taaattagcc
     9421 gctccctgcc taagatacct cttaatagat aagtcaaggc catggacatt attgtctaca
     9481 aggcatgttt caaagacatg accagtcagg acacttctgt catactccat gttgccccct
     9541 agtacacagt actaatctga tatctctgtt cccgccatgc ctgggggata aaatgatagc
     9601 agagactcct ttccttcaat gtgatctaat tcccaacaaa atctgggcct gagataccac
     9661 ctgtttctat ggcaaacatc ctcagtaaag tgttattctc attgcagatt gttccagcct
     9721 aatgtaagag gaacagagca gtgttccctt ggagcctcat gtggacagtt ctacctgtag
     9781 tgaccagttg gctatagtag ttattagctg gaacaaccag acagggtaca tgccccctcc
     9841 aaaatccatg ttgtactccc ctctgccagc cagggggggt gagatctgta gaatagtgca
     9901 gccagtgaca agccaccttg tgtttgtcac cagctcaaaa actcatctaa ggttgggagc
     9961 aggcagacaa ggcagagaga aagatccagg acagacctag ctgggctgga ggggtcttga
    10021 aaagccctct gtcgtattca ccttcagttt ttgtgctttg ggacaattac tttagaaaat
    10081 aagtaggtcg ttttaaaaac aaaatattga ttgctttttt gtagtgttca aaacaaaagg
    10141 ttctttgtgt atagccaaat gactgaaagc actgatatat ttaaaaacaa aaggcaattt
    10201 attaaggaaa tttgtaccat ttcagtaaac ctgtctgaat gtacctgtat acgtttcaaa
    10261 aacacacccc actgaacccc tgtaacctat ttattatata aagagtttgc cttataaatt
    10321 tacataaaaa tgtccgtttg tgtcttttgt tgtaaaatca agtggttttt cataaggttc
    10381 ttttactatt tgaaaagatg ggcagcacgc ggtttcattt tatttttgta agttttttaa
    10441 tacatgtgaa agcaaagaat actcagcatg cctttctaag tgatgcgttt gcaccttttg
    10501 ttgggaagta ctgtatcctg tgctgttagc attctcgata aatctctctg tgaaagtgaa
    10561 aaaaaaaaaa aaaaaa
//
