LOCUS       NM_181407               4599 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus malic enzyme 3, NADP(+)-dependent, mitochondrial
            (Me3), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_181407
VERSION     NM_181407.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Brunskill,E.W., Aronow,B.J., Georgas,K., Rumballe,B.,
            Valerius,M.T., Aronow,J., Kaimal,V., Jegga,A.G., Yu,J.,
            Grimmond,S., McMahon,A.P., Patterson,L.T., Little,M.H. and
            Potter,S.S.
  TITLE     Atlas of gene expression in the developing kidney at microanatomic
            resolution
  JOURNAL   Dev Cell 15 (5), 781-791 (2008)
   PUBMED   19000842
  REMARK    Erratum:[Dev Cell. 2009 Mar;16(3):482. Yu, Jing [added]]
REFERENCE   3  (bases 1 to 4599)
  AUTHORS   Sauer,L.A. and Dauchy,R.T.
  TITLE     Identification and properties of the nicotinamide adenine
            dinucleotide (phosphate)+-dependent malic enzyme in mouse ascites
            tumor mitochondria
  JOURNAL   Cancer Res 38 (6), 1751-1756 (1978)
   PUBMED   25711
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC164643.4 and AC165077.3.
            
            On Jan 6, 2023 this sequence version replaced NM_181407.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK032196.1, BC099935.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-649               AC164643.4         185893-186541       c
            650-783             AC164643.4         82225-82358         c
            784-933             AC164643.4         79030-79179         c
            934-1009            AC164643.4         32485-32560         c
            1010-1171           AC164643.4         22205-22366         c
            1172-1275           AC164643.4         12236-12339         c
            1276-1385           AC165077.3         90735-90844
            1386-1483           AC165077.3         102833-102930
            1484-1597           AC165077.3         103238-103351
            1598-1703           AC165077.3         104962-105067
            1704-1846           AC165077.3         105646-105788
            1847-2020           AC165077.3         106632-106805
            2021-2119           AC165077.3         107371-107469
            2120-4599           AC165077.3         108774-111253
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 50.27 cM"
     gene            1..4599
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
                     /note="malic enzyme 3, NADP(+)-dependent, mitochondrial"
                     /db_xref="GeneID:109264"
                     /db_xref="MGI:MGI:1916679"
     exon            1..649
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             467..2281
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
                     /EC_number="1.1.1.40"
                     /note="NADP-ME"
                     /codon_start=1
                     /product="NADP-dependent malic enzyme, mitochondrial"
                     /protein_id="NP_852072.2"
                     /db_xref="CCDS:CCDS21443.1"
                     /db_xref="GeneID:109264"
                     /db_xref="MGI:MGI:1916679"
                     /translation="MGAALGTGARLTSVPRIACSSLRRQAPSAPAQGCHSKSGPPRPV
                     PLKKRGYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYENQQS
                     DLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFI
                     TIHDKGHIATMLNSWPEDNIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGV
                     NPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVRGEEYDDLLDEFMQAVTDKFGINCL
                     IQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITKNRLSNHVFVF
                     QGAGEAAMGIAHLLVMALEKEGIPKTEAIKKIWMVDSKGLIVKGRSHLNHEKEMFAQD
                     HPEVNSLEEVVRLVKPTAIIGVAAIAGAFTEQILRDMASFHERPIVFALSNPTSKAEC
                     TAEKCYRVTEGRGIFASGSPFKSVTLEDGRTFTPGQGNNAYVFPGVALGVIAGGIRHI
                     PDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAVKVLDYAYKHNLASYYP
                     EPKDKEAFVKSLIYTPDYDSFSLDTYSWPKEAMSVQKV"
     exon            650..783
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            784..933
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            934..1009
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1171
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1172..1275
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1385
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1386..1483
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..1597
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1598..1703
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..1846
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1847..2020
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2021..2119
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2120..4599
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2324..2329
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
                     /note="hexamer: TATAAA"
     polyA_site      2347
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
                     /note="major polyA site"
     regulatory      4517..4522
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
                     /note="hexamer: AGTAAA"
     polyA_site      4542
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
     regulatory      4556..4561
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
                     /note="hexamer: AATACA"
     polyA_site      4599
                     /gene="Me3"
                     /gene_synonym="1700020C08Rik; B230207H15Rik"
ORIGIN      
        1 agtcccgtga gctagcactg cctgtgaggt gctgcctttg gccagagagt gacaggggac
       61 agtagcgcct tgccccacct gctcgcagtg ggactgcata gacttcagct tctccagtta
      121 ggcaggtaag cggaagaagg agaaaccaag gttaaccaca ggtactgagg gtgcggggac
      181 tccttggaga gtattctccc atatgggggc agggaggcac gggctgtcat ccgcacggga
      241 tctgctttct gggcttgctt tcgctttgct ggctgagggg tgagcatcgc aaccaagcga
      301 ggagcaggtg gcctcggaca aggggaggag gctagcgcct tccatcagga acccccagcc
      361 tctccgagag ctcaggtccg gcgccctgct ggcgcgcagg acctggcttg cctagagggc
      421 acccctgacc ttggtctcgc cctggttcca ggtctgtgcc agggctatgg gcgccgcgct
      481 gggcactggc gcgcggctga cctccgtgcc tcgcatagcc tgcagcagtc ttcggcgcca
      541 ggcaccttcc gcgcccgccc agggctgcca ctccaagtct ggcccgcctc gacccgtacc
      601 cctgaagaag cgcggatacg atgtcaccag gaacccccat ctcaacaagg gcatggcctt
      661 tacacttgaa gagaggctgc agctcggcat ccacggcctg atcccccctt gcttcctcag
      721 ccaggatgtg cagctcctcc gcatcatgag atactatgag aatcagcaga gcgacctgga
      781 caagtacatc attctcatga ctctccaaga ccgcaacgag aagctcttct accgggtgtt
      841 gacctcagac gtggagaagt tcatgcccat tgtgtacaca cccactgtgg ggctggcctg
      901 tcagcactac ggcctcacct tccgccgacc ccgtgggctg ttcatcacca ttcatgacaa
      961 gggccacatt gcaacaatgc tgaactcttg gccagaagac aatattaagg ctgtggtggt
     1021 gactgatgga gagcgcatac tgggcttggg agacctgggc tgctacggca tgggcatccc
     1081 tgtgggcaag ctggccctgt acacagcatg tggaggggtg aatccacagc agtgccttcc
     1141 tgtcctgcta gatgttggca ccaacaatga ggagcttctt agagaccctc tgtacatcgg
     1201 cctgaaacac cagcgtgtcc gtggggagga gtatgatgac ctgctggatg aattcatgca
     1261 ggcggtgaca gataagttcg gaataaattg cctcatccag ttcgaagact ttgccaatgc
     1321 caatgccttc cgtctactca acaaataccg caacaagtac tgtatgttca atgatgacat
     1381 ccaaggcaca gcctctgtcg ctgtggcagg gatcctggct gctctgagaa tcaccaagaa
     1441 cagactgtcc aatcatgtgt ttgttttcca gggtgcgggt gaggcagcca tgggcattgc
     1501 ccacctcctt gtcatggccc tggaaaagga aggcataccc aagacagagg ccataaagaa
     1561 gatctggatg gtggactcca agggtctgat tgtcaaggga agaagccacc taaaccatga
     1621 gaaggagatg tttgcccaag accaccctga agtcaactcc ctggaggagg tagtaaggct
     1681 ggtgaagccc acagcaatta taggtgtcgc tgccatcgca ggagccttca cggagcagat
     1741 tctgagggac atggcttcct tccatgagcg ccctatcgtc tttgccctga gcaaccccac
     1801 cagcaaggct gagtgcacag ctgagaagtg ctaccgggtc accgagggcc gaggaatctt
     1861 tgccagtgga agtcctttta agagtgtgac tctggaagat ggcaggacat tcactcctgg
     1921 tcagggaaac aatgcctatg tgtttcctgg ggtagctctg ggtgtcatcg ctggtgggat
     1981 ccggcacatc ccagatgaaa tcttcctcct gacggcagag caaattgccc aagaagtctc
     2041 tgagcagcac ctgtctcagg ggagactcta cccaccactc agcaccatcc gagatgtgtc
     2101 tctgagaatt gcagtcaaag tcctcgacta tgcatacaaa cacaacttgg cctcctacta
     2161 tccagagccc aaggacaagg aggcttttgt aaaatctctg atctacactc cagactatga
     2221 ctccttctca ctagacacct acagctggcc taaggaagct atgagtgttc agaaggtctg
     2281 acgcagtctc cagagatggt ttgggctgga tgaagaaata caatataaac cggttccaaa
     2341 atggccagtc ttgtctctgt ggttatattt gtcatttgtc ttcctttggg tttgggggta
     2401 gatgctaagg caaacagcga tgatagcttt gttcctgaag gccctggaga ctcattttcc
     2461 tataaccttt gtctgaattc ctttctgaat atctctttta ctattaattg ctatctacta
     2521 atcaaacctt ctggaaattt ctgcctagaa cctcaccttt gtagaggtaa aggtctagat
     2581 tggacatgag tcctttttca taagagagaa agtgttatgt ccatgaaaaa aaaaagttaa
     2641 tatagaagac agcatgctcg gaggagaatg gacacttcct ataaagacaa aaaggagagc
     2701 tggctttgta ctttgcccca agaataaagg gtgggttaag tcataaacac tcaagaaatg
     2761 caagcaaagc tacttgctag aaaatgaggt ccactcctca ggaagttaat ccctttcaga
     2821 agaatatatt accttcaaga ctcagtagga tgtggggggc tgagtggcta gacgttcatc
     2881 aagagggctc actgcccttt gggttgttga gtacattctt ttttgtcatc actaaaagta
     2941 gagaaatagg ggtgggaagg gtcagcatgc caaagactga ggaaaaggag acgcagtgag
     3001 agtaaacacc gtgaggaagg tcagccccgt tagatttaat gtctaacaaa gacgtgattt
     3061 aatgttccga gttctggaac agattctcct tcccatcctt cacactggca taactgagca
     3121 agtcaaagca gtttgcttgt agagatgacg tcagtctgac cttggtgttt tggaggccag
     3181 tttagtgaaa acttctacct ctcccatgtt gagaagtact cacactccaa gacaaacagt
     3241 aaagaacagc ttggagcccc tgctgtgacc catctcattt cactgtataa gtcatgctta
     3301 agacctaccc ctgctgtctc ctcggccagg attcctgctg cctataagag taggtccgat
     3361 ttgactgtct ctcagttcaa gccaggtaaa ctataaagat ccatggagag aatttagcag
     3421 attcatgaat caatcatccc ttgtttgggt cttattcttc cactatcagc ttcctttggt
     3481 gctagttctc agccaacaat ctccccaaag catagaccta aatgcagaaa gtaaaactct
     3541 acatttctcg aagagaataa agcagtcagt ccttgtgaag atcccccagt aatctctgct
     3601 tacaatctca catgcaatga ctgcatccct gggaatacaa ctcgcaaaag atggaagaca
     3661 ctggctaagg tctccaatgt tctgatctat tgcatcagtc ccacatggcc gccaagagcc
     3721 ctgtcggatt tctcatttcc tcaggagaca ccatctgcct agacccagct taatcctttt
     3781 tattccaact ccagaatcag taaatatacc cagagaagac aattgctgct tatatcagaa
     3841 aggctccctc cacagaatca gatactgcta gtcgatattc ttctcagagt tctcacatat
     3901 ggctttggaa atgtcttcct gttttttttt tttaattggt gcaggtagtg tcttggggtt
     3961 ccatcatcta atctactata tcctatgcag agaattagaa gtttctagac atagattttc
     4021 aaaattccag atatattctt tttttcaaaa acaattttta ttagatattt tcttcattta
     4081 catttcaaat gctatcctga aagtccctta taccctcccc ccaccttgct ccccaaccca
     4141 tccactccca cttcctggcc ttggcattcc cctgtatata ttcttaatac cttaatacct
     4201 taattgaatt atattcaaag aatatttggg ggaaattttt gtcaaatgtt atgatcaaat
     4261 ttaagaagac tgttcacatc ctcttacaaa ctgatatgta caccaacatg gttgcatgaa
     4321 ataccttttg tttggggggg ttattatttt aaccatttct atacattact gaattttgtg
     4381 tctactcaac ctatttctga aagataagca gagttatggg aaacttcaat tttccttgtg
     4441 tctctgacca tatttacttt atatcttttg gcccagtgtt attaggtgta cgaagtttag
     4501 aattgttata acttatagta aactcaaaac tgtaaacctt tatgatttac ttaataatac
     4561 atttgtttta tcgtgccaga tcttggatat cataccata
//
