LOCUS       NM_145494               7587 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus malic enzyme 2, NAD(+)-dependent, mitochondrial (Me2),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_145494
VERSION     NM_145494.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7587)
  AUTHORS   Liu,F., Jiang,L.J., Zhang,Y.X., Xu,S.T., Liu,S.L., Ye,J.T. and
            Liu,P.Q.
  TITLE     Inhibition of miR-214-3p attenuates ferroptosis in myocardial
            infarction via regulating ME2
  JOURNAL   Biochem Biophys Res Commun 661, 64-74 (2023)
   PUBMED   37087800
  REMARK    GeneRIF: Inhibition of miR-214-3p attenuates ferroptosis in
            myocardial infarction via regulating ME2.
REFERENCE   3  (bases 1 to 7587)
  AUTHORS   Li,W., Kou,J., Zhang,Z., Li,H., Li,L. and Du,W.
  TITLE     Cellular redox homeostasis maintained by malic enzyme 2 is
            essential for MYC-driven T cell lymphomagenesis
  JOURNAL   Proc Natl Acad Sci U S A 120 (23), e2217869120 (2023)
   PUBMED   37253016
  REMARK    GeneRIF: Cellular redox homeostasis maintained by malic enzyme 2 is
            essential for MYC-driven T cell lymphomagenesis.
REFERENCE   4  (bases 1 to 7587)
  AUTHORS   Wang,Y.P., Sharda,A., Xu,S.N., van Gastel,N., Man,C.H., Choi,U.,
            Leong,W.Z., Li,X. and Scadden,D.T.
  TITLE     Malic enzyme 2 connects the Krebs cycle intermediate fumarate to
            mitochondrial biogenesis
  JOURNAL   Cell Metab 33 (5), 1027-1041 (2021)
   PUBMED   33770508
  REMARK    GeneRIF: Malic enzyme 2 connects the Krebs cycle intermediate
            fumarate to mitochondrial biogenesis.
REFERENCE   5  (bases 1 to 7587)
  AUTHORS   Dey,P., Baddour,J., Muller,F., Wu,C.C., Wang,H., Liao,W.T., Lan,Z.,
            Chen,A., Gutschner,T., Kang,Y., Fleming,J., Satani,N., Zhao,D.,
            Achreja,A., Yang,L., Lee,J., Chang,E., Genovese,G., Viale,A.,
            Ying,H., Draetta,G., Maitra,A., Wang,Y.A., Nagrath,D. and
            DePinho,R.A.
  TITLE     Genomic deletion of malic enzyme 2 confers collateral lethality in
            pancreatic cancer
  JOURNAL   Nature 542 (7639), 119-123 (2017)
   PUBMED   28099419
  REMARK    GeneRIF: deletion of malic enzyme 2 confers collateral lethality in
            pancreatic cancer
REFERENCE   6  (bases 1 to 7587)
  AUTHORS   Jiang,P., Du,W., Mancuso,A., Wellen,K.E. and Yang,X.
  TITLE     Reciprocal regulation of p53 and malic enzymes modulates metabolism
            and senescence
  JOURNAL   Nature 493 (7434), 689-693 (2013)
   PUBMED   23334421
  REMARK    GeneRIF: p53 represses the expression of the
            tricarboxylic-acid-cycle-associated malic enzymes ME1 and ME2 in
            human and mouse cells
REFERENCE   7  (bases 1 to 7587)
  AUTHORS   MacDonald,M.J., Longacre,M.J. and Kendrick,M.A.
  TITLE     Mitochondrial malic enzyme (ME2) in pancreatic islets of the human,
            rat and mouse and clonal insulinoma cells
  JOURNAL   Arch Biochem Biophys 488 (2), 100-104 (2009)
   PUBMED   19691144
  REMARK    GeneRIF: Me2 activity in Islet of Langerhans cells was measured by
            a spectrophotometric enzyme assay by utilizing the distinct kinetic
            properties of Me2.
REFERENCE   8  (bases 1 to 7587)
  AUTHORS   Moreadith,R.W. and Lehninger,A.L.
  TITLE     Purification, kinetic behavior, and regulation of NAD(P)+ malic
            enzyme of tumor mitochondria
  JOURNAL   J Biol Chem 259 (10), 6222-6227 (1984)
   PUBMED   6725250
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC132418.3.
            
            On Jul 21, 2018 this sequence version replaced NM_145494.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK050933.1, AK156403.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164133, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-135               AC132418.3         16893-17027
            136-253             AC132418.3         27162-27279
            254-387             AC132418.3         30469-30602
            388-537             AC132418.3         34353-34502
            538-613             AC132418.3         35942-36017
            614-775             AC132418.3         37389-37550
            776-879             AC132418.3         37782-37885
            880-989             AC132418.3         40421-40530
            990-1087            AC132418.3         40625-40722
            1088-1201           AC132418.3         41134-41247
            1202-1316           AC132418.3         43863-43977
            1317-1459           AC132418.3         46494-46636
            1460-1562           AC132418.3         51187-51289
            1563-1633           AC132418.3         56882-56952
            1634-1732           AC132418.3         59103-59201
            1733-7587           AC132418.3         61523-67377
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 49.95 cM"
     gene            1..7587
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /note="malic enzyme 2, NAD(+)-dependent, mitochondrial"
                     /db_xref="GeneID:107029"
                     /db_xref="MGI:MGI:2147351"
     exon            1..135
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /inference="alignment:Splign:2.1.0"
     exon            136..253
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /inference="alignment:Splign:2.1.0"
     CDS             146..1915
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /EC_number="1.1.1.38"
                     /note="NAD-dependent malic enzyme, mitochondrial"
                     /codon_start=1
                     /product="NAD-dependent malic enzyme, mitochondrial
                     precursor"
                     /protein_id="NP_663469.1"
                     /db_xref="CCDS:CCDS29339.1"
                     /db_xref="GeneID:107029"
                     /db_xref="MGI:MGI:2147351"
                     /translation="MFSRLRAVTTPCTLTCRRVHLKEKGKPLMLNPRTNKGMAFTLQE
                     RQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMGIQERNEKLFYRILQD
                     DIESLMPIVYTPTVGLACCQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVV
                     TDGERILGLGDLGVYGMGIPVGKLCLYTACAGIQPEKCLPVCIDVGTDNMALLKDPFY
                     MGLYQKRDRSQLYDDLMDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYQQKYCTF
                     NDDIQGTAAVALSGLLAAQRVINKPVSEHKILFLGAGEAALGIANLIVLSMVESGLSE
                     EEAQRKIWMFDKSGLLVKGRTASIDSNQEPYAHAAPESIPATFEDAVNKLKPSVIIGV
                     AGAGPLFTHGVIKAMASINERPIIFALSNPTAQAECTAEDAYTLTEGRCLFASGSPFE
                     PVKLQDGRVFTPGQGNNAYIFPGVALAVILCEARHISDTVFLEAAKALTTQLTDAELA
                     QGRLYPSLANIQEVSANIAIKLAEYLYANKMAFRYPEPEDKARYVRERIWRSNYVSLL
                     PDVYDWPESSLTPPQITEEKLPH"
     exon            254..387
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /inference="alignment:Splign:2.1.0"
     exon            388..537
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /inference="alignment:Splign:2.1.0"
     exon            538..613
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /inference="alignment:Splign:2.1.0"
     exon            614..775
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /inference="alignment:Splign:2.1.0"
     exon            776..879
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /inference="alignment:Splign:2.1.0"
     exon            880..989
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1087
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1201
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1316
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /inference="alignment:Splign:2.1.0"
     exon            1317..1459
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1562
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /inference="alignment:Splign:2.1.0"
     exon            1563..1633
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1732
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /inference="alignment:Splign:2.1.0"
     exon            1733..7587
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1971
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /note="major polyA site"
     regulatory      2477..2482
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /note="hexamer: AATAAA"
     polyA_site      2501
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
     regulatory      7560..7565
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /note="hexamer: AGTAAA"
     polyA_site      7585
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
ORIGIN      
        1 agagcgagcc ggagctggcg ccggctgcca ggagctcgcg ggaggctggc agggtggcgc
       61 cagggccgag catctccggc tgtagcgccg cggcctctct gcttgctgca ccacctggca
      121 cgggccaggc gtccggggag agaagatgtt ttcccggcta agagctgtta ccactccttg
      181 taccttgacc tgccgccgag tgcacctgaa agagaagggc aagccactca tgttgaatcc
      241 aagaacaaac aagggaatgg cgtttacgtt acaagagcga caaatgcttg gtcttcaagg
      301 acttttacct cctaaaatag agacacagga tattcaagcc ttacgattcc atagaaactt
      361 gaaaaaaatg actagccctt tggaaaaata catttatata atgggcatcc aagaaagaaa
      421 tgagaaactg ttctatagaa tcctgcagga tgatattgaa agtctgatgc cgattgtgta
      481 cacgccaaca gttggtcttg cctgctgtca atatgggcac atctttagga gacctaaggg
      541 attgtttatt tcaatctcag acagaggtca tgttagatca attgtggata actggccaga
      601 aaatcatgtt aaggctgttg tggtgactga tggagagagg atcctgggcc ttggcgatct
      661 gggtgtctat ggaatgggga ttccagtagg gaagctgtgc ctgtatacag cctgtgcagg
      721 gatacagccc gagaagtgcc tgcccgtgtg catcgacgtg ggcactgata acatggcact
      781 attaaaagat ccgttttata tgggcttgta tcagaagcga gatcgttccc agctctatga
      841 tgatctgatg gacgagttta tgaaggcgat tactgacaga tacggccgga acacactcat
      901 tcagtttgaa gactttggaa atcataatgc attcaggttc ttaagaaaat atcaacaaaa
      961 atactgtacc ttcaatgatg acattcaagg gacagctgca gttgctctgt ctggtcttct
     1021 tgcggcccaa agagttatta ataaaccagt ctcagaacac aaaatcttat ttcttggagc
     1081 aggagaagct gcacttggaa ttgccaatct catagttcta tcaatggtgg aaagtggtct
     1141 ctcagaagag gaggcgcaga gaaagatctg gatgtttgac aagagtggct tgctggttaa
     1201 ggggcggact gctagcatag atagcaacca ggaaccatat gcccatgcgg ccccagagag
     1261 catacctgcg acttttgaag atgcagtaaa taaacttaag ccttcagtta taattggagt
     1321 ggcaggtgcc ggcccgcttt ttactcacgg tgtaatcaaa gccatggcct ctatcaacga
     1381 gagacctata atatttgctc taagtaatcc tacagcacag gccgagtgca cagccgagga
     1441 cgcgtacacg ctcacagagg gcaggtgtct gtttgccagt ggcagcccat ttgagccagt
     1501 gaaactccag gatggacgag tcttcacccc aggccaagga aacaatgcat atattttccc
     1561 aggtgtggct ttagctgtta ttctctgtga agcccgacac atcagtgaca ccgttttctt
     1621 agaagctgca aaggcgctga cgactcagtt gacagatgca gagttagccc aagggaggct
     1681 ttacccgtcg ctggctaata ttcaggaagt ttctgctaac atagctatta aacttgcaga
     1741 gtacctgtac gctaataaaa tggccttccg gtacccagaa cccgaggaca aggccagata
     1801 cgtgagagag agaatatggc ggagcaacta cgtttccctg ctgccggacg tgtatgactg
     1861 gccagagtct tcactgacac ctcctcagat cacagaagag aagcttcccc actgagacgc
     1921 tccggccttc agggaaccac tatttttaga caaaaagata atattctctg agttacggtg
     1981 tcatctgtgg tttattcttc cgtacccact ttgcttggct tttttttttt ccctgtgaat
     2041 ctcagtttcg ttggggacag acatgctggc tatactgtag tgcccacagc ccagttctgg
     2101 gaatgcgtcc gttccgatgg gtcgcctcct gcgctctgct ttaagcttgc gacggtactg
     2161 tcgctgttaa cgtgaatgta tctctcactt cccccctcag ccctccagtg ctgtgcacgt
     2221 gagcacagaa cgttgagaag gagtgagtgc tcaccgaatt ccaaagaaaa cttgtcagca
     2281 ccaagaaact gctctgttct tgtcactttc tgctctgacg tccctttgta gttagacaga
     2341 tttggggaaa tacaaaaaaa aacttcctag aactaaatca gtgctaacta gcattataga
     2401 agagtgggag gagccactgc tgcctttaga caaactggac tgtcacgtgc aacagtgtac
     2461 aaaacccact tttattaata aaagtaattc cttacattta attgcttcag ttacattatc
     2521 ttattgacca aacagaaact tacgtttgtt gattgtgaga acagtttcat cgtctttctc
     2581 tgctgagaat ggataatagg ttccgagctt agtggagaat aaataaatga actagaccag
     2641 gaaatgcctt cttttgtatt ttcaagacag gatctcacta tgtagccctg gcattcctgg
     2701 aactcactct gtagaccagg ccagcctcga actcagagat cctcctgcct ctgcctccgg
     2761 agtgctggga attgaggacg tacactgctc cccctggact gtacacctgg caggaaatgc
     2821 ctttaaaaaa aaaaaaaaaa gatttattta tttgagtaca ctgtaactgt cttcagacac
     2881 accagaagag ggcattagat cccatttcag atggttgtga gtcaccatgt ggttgctggg
     2941 aattgagctt agggcctcta aaaaagcaat cagtgctctt aaccactgag ctatctcccc
     3001 agcccaaccc aggaagtact atctttcaat ccaagggtaa catcagtgtt catgagtcat
     3061 gttctgaaag aaccctgaag tttgttggag aaatgttagt gtttgtggca ttcacctgtc
     3121 tagatgcttt gagggacaaa gtttgttctt cttttgagat tttagcaagc tgacctgaaa
     3181 ctcactctgt gtccctgact ttcttccatg catgtgttgt tatcatagcc atgaaccaca
     3241 atggtcagga acaaaactta aaaatgctca ccccagaatg gtggcagtac agagcggttt
     3301 gtaggtacat accagtgtgt gtttatactc tataggcaaa ggggacttct tcccatcttc
     3361 ctgcttaaga ttgacttgaa gttctgaagg tgtgttcaga ctgcttagtg tacacgacct
     3421 tgggttcagt ccacagggcc accccagaga ggcagtctta caatggcatt gcagttttaa
     3481 gtatgggtgg acacgatggt taatttaaca taagtagcag tctgttcaat acaataatga
     3541 agtaaatcat tgattgaaag atgaatacac aggtacgagt ctagtactga agcaggagaa
     3601 tctcaacttc aaagccagaa tgtctacaaa agaactcaca atgtgattgc aagatggttc
     3661 agtggttagg gctcttgtgt ctctgagcct ccacatttaa gtttaattcc aggacccata
     3721 tggtggacag aaagaaccaa tttccagaag ttattttctg attaccctcc cccgccccca
     3781 cttgccacac acacacacac cataaataaa tgaacaaaaa tgaagagaaa cgtttatttt
     3841 ttttcatgtt tttatttaat tctgtctatg aaatctaaat attgaatgtt acaagtatac
     3901 aaatgtgttg ctggtttttt gggttttttt gctcagtttg ttaacggtcg ggacagaggc
     3961 tgagttacag cacttttact aagttgttac ttactggatg gtacaagtca cagcaaccat
     4021 tttaaagatg gcaagataac atatagcctg cttataaaca ttatctaatg taaatttaaa
     4081 tgtttattat ctaagagtgt gggcaatggg agaccctgat ggcgtcgaat tgggtgtggg
     4141 ggagggagtt actggggctc tccatgggga attgtgccaa ccaagaaggt cattatttgg
     4201 aggagtggtg atggtgcctc acctgctggg caaaggaagg gctggacacg agtttccagc
     4261 agtcaggagg aaaagggccc ctgctctctt cccactgtga agcagagctg ggaaacttga
     4321 tgagggcata gtcggtgcac ggatggtcag ctctgagggt ttcattaaac cattttccaa
     4381 gggtggcttc cccaccagaa ggttctctca gaatcactgt gaacccctta agtggagatt
     4441 tccttgtctt cagagcccta agtgacctgg gggggggggg gggagaagga ttctgatggc
     4501 atcttgttct cacacactaa ggccttggtg tgggccggca ggtagccgtg ttgctgctgg
     4561 tacagtttca gggtggggtt cactatctag tgtttcttcg tttctttttt attaaatctt
     4621 ttttttcatg tatgttgatt ttgtacattt ctcaacctcc cagatctgcc tacacactca
     4681 acttcttgtt ctttctcttt gtctctattt ctcaacaaca acaaaaacca gaaaagatgg
     4741 aatgtcacta gggcagtccc caagcctagg agtagctggc caaatgcaaa cacagcccat
     4801 gggttttgtc tgggcgtgtg tgtgcttttg gttccggttt ttggtttggg aggttttggt
     4861 ggtggtggtg gtggtggtgg tagtttggtt tggtttggtt tggtttttgt ctggttggtt
     4921 ggttggttgg ttggttggtg aggtctcacg tgtctaatga tccccttggt gcccaggctg
     4981 ttggtgcaga tgcaaaactg agcatgttgc attgcacctt tgaactgagt gctgagaaac
     5041 tgtgtgcttc agtttatatg aaatcatata gaatggcttt catctagttg tctttaattg
     5101 atattggata ttaagttaat aaggaaactt tagacataat ttttctgaaa acatataatc
     5161 taggatactt tattaagctc ataaaatcta gtgaccaggt tgttttgttg ttgttgtttt
     5221 gttttgtttt gttttgtttt gtttgtaaga gtagctatcc actgtctatt tatggaacag
     5281 ttttctttga ggaaggcctt gatgattttt ctctaactta ctggcttctg aagagatttt
     5341 acccagttac cactggattt agactgctct ttccaggagt gcagagtgga gcagtagtca
     5401 tttgttccca cggggagtgg agacggtgca gggagcctgg ccgtggagga agtacagatg
     5461 tgtcacaatg gggtcagtgt tctccagctc tcctaaccaa aatcagccct aaggagcatt
     5521 caccatagtc aagaacatta atacaaacct gagagctccc ttactggagt tccatgtcca
     5581 atcctcattg ggctagcagc agcatccagg ttccattcaa gacgtgttcc ggtgcagacc
     5641 tgttgctgac tgcacacagg agacaggtct catcagccac tgtgatttgc acttgtacac
     5701 agtgttaaaa tgaaacctgc tagccaggta tgctgcatgc ctctagtccc agcacttggc
     5761 aggtagaagc aggaggatcg ggagttaagg ctagcttcaa ctacatatgg agatcagggc
     5821 tagcctgggc tacaggagac cctgcctcaa caaagaaagc cctcaaagga tgagctgaag
     5881 ttatacacta aataagtaaa tattcaaaat aatctagcct tagaaaacca cttcatctga
     5941 agtcaatgtc ttacttcgtg cttgaagaaa gagtgctgtt ttttatttat ttcaaatttc
     6001 aaggcattca gataatcaag aggggacatg cgttgtggaa ctgtctgatc tttattatga
     6061 tttacttgga gtaatacttg gattttaaag caactactta aaattgtgtc cctgaaaagg
     6121 aaaaggatgg cgttcacagt tggttgtgtt ccctgcatgg ttaattttac ctgtgtgtct
     6181 agaggaaggc ccaggctagt gcgtgcatta agctagtgcg tgcgtatctt cggctaggtg
     6241 tcatttccca atgactgaac tctcttcatc tttttcttcc aattaaaaat gtatgtgccg
     6301 tttgctcctt aagacattcc atacccttaa gtcttagact cggaggggtc tgtcggtctg
     6361 ttggtctgcc cgtctgtatc ttcagttctc aggagttttc tttgcttgtg tgctcatttg
     6421 tgtgtttgag gcagggccac ataaccgagt ccagccttac aacttgcgac ttagcctcac
     6481 ctgtgctgaa agagtctgcg tacatcctag aaactttatt ttctagaaat tcaccgattc
     6541 tcatgtggtc attgagtgcc accagtacat ctgatggatc cagttggcac gtcatgacag
     6601 attgacttgt gtgactgtgc ctgctctgtg tcgggtgttc aaagcatctc ccatgtacgc
     6661 tggagccctg catctctgac cctgttttac aagcaagggc agcgccacag gggagttgaa
     6721 gagctcttgt gagctcagcc ccttcagtct ctgcaggcag ttctagccaa ggggacagtc
     6781 ttcttcgcct gtttggacaa gattagttct cagtcctttg gaatgcgttt tcccagttcg
     6841 taggattttt cccccctttg ttcctactta acgattcaaa cacactataa acctcttact
     6901 tgttaattat tagatgtata tattaagtta tttgttttga atgtgataga ttacgccaaa
     6961 gattctcagt agctagttca atcttgattt agtaatattc atagagccac cggctgccgt
     7021 cactgttcta caaatcagtg tcctccgcag tcagtcatcc actttgcgac tctagtcctg
     7081 tccccgcgcc cccagaaggt attctctgat cgaagtctgt tatgtgtact tccatgaagg
     7141 aggactatgt gcagaatgaa gagagctatc ttagctgaac atttgacaaa attcctaccc
     7201 agtgttttga ttgtactttg cctgataagt tttaaaatca ccagaaagta aaacaatttt
     7261 tacagttcat gtcgtcgtcg tcatcgaaga aaccacacat cctgtgtcat tgcttagcat
     7321 ttctcactgt gtgtgtgtgt tggggtgggg gggggcggat gtgggaatga caggtttttc
     7381 agaggtgatt ccctcatggg aagataaagg ggaatgcatg ctctctgtag agtatttcat
     7441 gctgtgttga cttcttacgg agagtcccga gactgctata aagacttgat aattacgtga
     7501 actgtttttc cctttttgtg atatattcct tgtaatgaga tgtgttctta tattttctta
     7561 gtaaaaattt tcttatgtgt aaaaatg
//
