LOCUS       NP_663469                589 aa            linear   ROD 28-APR-2025
DEFINITION  NAD-dependent malic enzyme, mitochondrial precursor [Mus musculus].
ACCESSION   NP_663469
VERSION     NP_663469.1
DBSOURCE    REFSEQ: accession NM_145494.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 589)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 589)
  AUTHORS   Liu,F., Jiang,L.J., Zhang,Y.X., Xu,S.T., Liu,S.L., Ye,J.T. and
            Liu,P.Q.
  TITLE     Inhibition of miR-214-3p attenuates ferroptosis in myocardial
            infarction via regulating ME2
  JOURNAL   Biochem Biophys Res Commun 661, 64-74 (2023)
   PUBMED   37087800
  REMARK    GeneRIF: Inhibition of miR-214-3p attenuates ferroptosis in
            myocardial infarction via regulating ME2.
REFERENCE   3  (residues 1 to 589)
  AUTHORS   Li,W., Kou,J., Zhang,Z., Li,H., Li,L. and Du,W.
  TITLE     Cellular redox homeostasis maintained by malic enzyme 2 is
            essential for MYC-driven T cell lymphomagenesis
  JOURNAL   Proc Natl Acad Sci U S A 120 (23), e2217869120 (2023)
   PUBMED   37253016
  REMARK    GeneRIF: Cellular redox homeostasis maintained by malic enzyme 2 is
            essential for MYC-driven T cell lymphomagenesis.
REFERENCE   4  (residues 1 to 589)
  AUTHORS   Wang,Y.P., Sharda,A., Xu,S.N., van Gastel,N., Man,C.H., Choi,U.,
            Leong,W.Z., Li,X. and Scadden,D.T.
  TITLE     Malic enzyme 2 connects the Krebs cycle intermediate fumarate to
            mitochondrial biogenesis
  JOURNAL   Cell Metab 33 (5), 1027-1041 (2021)
   PUBMED   33770508
  REMARK    GeneRIF: Malic enzyme 2 connects the Krebs cycle intermediate
            fumarate to mitochondrial biogenesis.
REFERENCE   5  (residues 1 to 589)
  AUTHORS   Dey,P., Baddour,J., Muller,F., Wu,C.C., Wang,H., Liao,W.T., Lan,Z.,
            Chen,A., Gutschner,T., Kang,Y., Fleming,J., Satani,N., Zhao,D.,
            Achreja,A., Yang,L., Lee,J., Chang,E., Genovese,G., Viale,A.,
            Ying,H., Draetta,G., Maitra,A., Wang,Y.A., Nagrath,D. and
            DePinho,R.A.
  TITLE     Genomic deletion of malic enzyme 2 confers collateral lethality in
            pancreatic cancer
  JOURNAL   Nature 542 (7639), 119-123 (2017)
   PUBMED   28099419
  REMARK    GeneRIF: deletion of malic enzyme 2 confers collateral lethality in
            pancreatic cancer
REFERENCE   6  (residues 1 to 589)
  AUTHORS   Jiang,P., Du,W., Mancuso,A., Wellen,K.E. and Yang,X.
  TITLE     Reciprocal regulation of p53 and malic enzymes modulates metabolism
            and senescence
  JOURNAL   Nature 493 (7434), 689-693 (2013)
   PUBMED   23334421
  REMARK    GeneRIF: p53 represses the expression of the
            tricarboxylic-acid-cycle-associated malic enzymes ME1 and ME2 in
            human and mouse cells
REFERENCE   7  (residues 1 to 589)
  AUTHORS   MacDonald,M.J., Longacre,M.J. and Kendrick,M.A.
  TITLE     Mitochondrial malic enzyme (ME2) in pancreatic islets of the human,
            rat and mouse and clonal insulinoma cells
  JOURNAL   Arch Biochem Biophys 488 (2), 100-104 (2009)
   PUBMED   19691144
  REMARK    GeneRIF: Me2 activity in Islet of Langerhans cells was measured by
            a spectrophotometric enzyme assay by utilizing the distinct kinetic
            properties of Me2.
REFERENCE   8  (residues 1 to 589)
  AUTHORS   Moreadith,R.W. and Lehninger,A.L.
  TITLE     Purification, kinetic behavior, and regulation of NAD(P)+ malic
            enzyme of tumor mitochondria
  JOURNAL   J Biol Chem 259 (10), 6222-6227 (1984)
   PUBMED   6725250
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC132418.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK050933.1, AK156403.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164133, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 49.95 cM"
     Protein         1..589
                     /product="NAD-dependent malic enzyme, mitochondrial
                     precursor"
                     /EC_number="1.1.1.38"
                     /note="NAD-dependent malic enzyme, mitochondrial"
                     /calculated_mol_wt=63705
     transit_peptide 1..18
                     /note="Mitochondrion.
                     /evidence=ECO:0000250|UniProtKB:P23368; propagated from
                     UniProtKB/Swiss-Prot (Q99KE1.1)"
                     /calculated_mol_wt=2113
     mat_peptide     19..589
                     /product="NAD-dependent malic enzyme, mitochondrial.
                     /id=PRO_0000018538"
                     /note="propagated from UniProtKB/Swiss-Prot (Q99KE1.1)"
                     /calculated_mol_wt=63705
     Region          28..566
                     /region_name="PLN03129"
                     /note="NADP-dependent malic enzyme; Provisional"
                     /db_xref="CDD:215594"
     Site            156
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:P23368; propagated from
                     UniProtKB/Swiss-Prot (Q99KE1.1)"
     Site            224
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:P23368; propagated from
                     UniProtKB/Swiss-Prot (Q99KE1.1)"
     Site            240
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:P23368; propagated from
                     UniProtKB/Swiss-Prot (Q99KE1.1)"
     Site            272
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:P23368; propagated from
                     UniProtKB/Swiss-Prot (Q99KE1.1)"
     Site            346
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:P23368; propagated from
                     UniProtKB/Swiss-Prot (Q99KE1.1)"
     CDS             1..589
                     /gene="Me2"
                     /gene_synonym="D030040L20Rik; NAD-ME"
                     /coded_by="NM_145494.3:146..1915"
                     /db_xref="CCDS:CCDS29339.1"
                     /db_xref="GeneID:107029"
                     /db_xref="MGI:MGI:2147351"
ORIGIN      
        1 mfsrlravtt pctltcrrvh lkekgkplml nprtnkgmaf tlqerqmlgl qgllppkiet
       61 qdiqalrfhr nlkkmtsple kyiyimgiqe rneklfyril qddieslmpi vytptvglac
      121 cqyghifrrp kglfisisdr ghvrsivdnw penhvkavvv tdgerilglg dlgvygmgip
      181 vgklclytac agiqpekclp vcidvgtdnm allkdpfymg lyqkrdrsql yddlmdefmk
      241 aitdrygrnt liqfedfgnh nafrflrkyq qkyctfnddi qgtaavalsg llaaqrvink
      301 pvsehkilfl gageaalgia nlivlsmves glseeeaqrk iwmfdksgll vkgrtasids
      361 nqepyahaap esipatfeda vnklkpsvii gvagagplft hgvikamasi nerpiifals
      421 nptaqaecta edaytltegr clfasgspfe pvklqdgrvf tpgqgnnayi fpgvalavil
      481 cearhisdtv fleaakaltt qltdaelaqg rlypslaniq evsaniaikl aeylyankma
      541 frypepedka ryvreriwrs nyvsllpdvy dwpessltpp qiteeklph
//
