LOCUS       NM_001302803            7220 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus transformed mouse 3T3 cell double minute 4 (Mdm4),
            transcript variant 4, mRNA.
ACCESSION   NM_001302803
VERSION     NM_001302803.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Yao,F., Huang,S., Liu,J., Tan,C., Xu,M., Wang,D., Huang,M., Zhu,Y.,
            Huang,X. and He,S.
  TITLE     Deletion of ARGLU1 causes global defects in alternative splicing in
            vivo and mouse cortical malformations primarily via apoptosis
  JOURNAL   Cell Death Dis 14 (8), 543 (2023)
   PUBMED   37612280
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7220)
  AUTHORS   Ueda,K.
  TITLE     Murine double minute X plays a central role in leukemic
            transformation and may be a promising target for leukemia
            prevention strategies
  JOURNAL   Exp Hematol 122, 10-18 (2023)
   PUBMED   37086813
  REMARK    GeneRIF: Murine double minute X plays a central role in leukemic
            transformation and may be a promising target for leukemia
            prevention strategies.
            Review article
REFERENCE   3  (bases 1 to 7220)
  AUTHORS   Alam,S.S., Kumar,S., Beauchamp,M.C., Bareke,E., Boucher,A.,
            Nzirorera,N., Dong,Y., Padilla,R., Zhang,S.J., Majewski,J. and
            Jerome-Majewska,L.A.
  TITLE     Snrpb is required in murine neural crest cells for proper splicing
            and craniofacial morphogenesis
  JOURNAL   Dis Model Mech 15 (6) (2022)
   PUBMED   35593225
REFERENCE   4  (bases 1 to 7220)
  AUTHORS   Bezzi,M., Teo,S.X., Muller,J., Mok,W.C., Sahu,S.K., Vardy,L.A.,
            Bonday,Z.Q. and Guccione,E.
  TITLE     Regulation of constitutive and alternative splicing by PRMT5
            reveals a role for Mdm4 pre-mRNA in sensing defects in the
            spliceosomal machinery
  JOURNAL   Genes Dev 27 (17), 1903-1916 (2013)
   PUBMED   24013503
  REMARK    GeneRIF: identify Mdm4 as one of these key mRNAs that senses the
            defects in the spliceosomal machinery and transduces the signal to
            activate the p53 response
REFERENCE   5  (bases 1 to 7220)
  AUTHORS   Wade,M., Li,Y.C. and Wahl,G.M.
  TITLE     MDM2, MDMX and p53 in oncogenesis and cancer therapy
  JOURNAL   Nat Rev Cancer 13 (2), 83-96 (2013)
   PUBMED   23303139
  REMARK    Review article
REFERENCE   6  (bases 1 to 7220)
  AUTHORS   Lenos,K. and Jochemsen,A.G.
  TITLE     Functions of MDMX in the modulation of the p53-response
  JOURNAL   J Biomed Biotechnol 2011, 876173 (2011)
   PUBMED   21541195
  REMARK    Review article
REFERENCE   7  (bases 1 to 7220)
  AUTHORS   Parant,J.M., Reinke,V., Mims,B. and Lozano,G.
  TITLE     Organization, expression, and localization of the murine mdmx gene
            and pseudogene
  JOURNAL   Gene 270 (1-2), 277-283 (2001)
   PUBMED   11404026
REFERENCE   8  (bases 1 to 7220)
  AUTHORS   Rallapalli,R., Strachan,G., Cho,B., Mercer,W.E. and Hall,D.J.
  TITLE     A novel MDMX transcript expressed in a variety of transformed cell
            lines encodes a truncated protein with potent p53 repressive
            activity
  JOURNAL   J Biol Chem 274 (12), 8299-8308 (1999)
   PUBMED   10075736
REFERENCE   9  (bases 1 to 7220)
  AUTHORS   Shvarts,A., Bazuine,M., Dekker,P., Ramos,Y.F., Steegenga,W.T.,
            Merckx,G., van Ham,R.C., van der Houven van Oordt,W., van der
            Eb,A.J. and Jochemsen,A.G.
  TITLE     Isolation and identification of the human homolog of a new
            p53-binding protein, Mdmx
  JOURNAL   Genomics 43 (1), 34-42 (1997)
   PUBMED   9226370
REFERENCE   10 (bases 1 to 7220)
  AUTHORS   Shvarts,A., Steegenga,W.T., Riteco,N., van Laar,T., Dekker,P.,
            Bazuine,M., van Ham,R.C., van der Houven van Oordt,W., Hateboer,G.,
            van der Eb,A.J. and Jochemsen,A.G.
  TITLE     MDMX: a novel p53-binding protein with some functional properties
            of MDM2
  JOURNAL   EMBO J 15 (19), 5349-5357 (1996)
   PUBMED   8895579
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CJ101093.1, AK017719.1,
            AK083349.1, AK043359.1, AK046448.1 and BC058680.1.
            
            Summary: This gene encodes a protein that has been shown to
            negatively regulate the activity of the tumor suppressor protein
            p53. Homozygous knockout mice exhibit embryonic lethality as a
            result of p53-dependent apoptosis and cell cycle arrest.
            Amplification of this gene or overexpression of the encoded protein
            has been linked to a range of human cancers. A pseudogene has been
            identified on the X chromosome. Alternative splicing of this gene
            results in multiple transcript variants. [provided by RefSeq, Nov
            2014].
            
            Transcript Variant: This variant (4) uses an alternate in-frame
            splice site in the 5' coding region, compared to variant 1. The
            resulting isoform (2) is shorter compared to isoform 1. Both
            variants 3 and 4 encode the same isoform (2).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN01164138,
                              SAMN01164141 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                CJ101093.1         1-50
            51-1845             AK017719.1         1-1795
            1846-3480           AK083349.1         1857-3491
            3481-5167           AK043359.1         910-2596
            5168-5929           AK046448.1         781-1542
            5930-7105           BC058680.1         1-1176
            7106-7220           AK046448.1         2719-2833
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 57.75 cM"
     gene            1..7220
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /note="transformed mouse 3T3 cell double minute 4"
                     /db_xref="GeneID:17248"
                     /db_xref="MGI:MGI:107934"
     exon            1..169
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     exon            170..277
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     CDS             200..1669
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /note="isoform 2 is encoded by transcript variant 4;
                     protein Mdm4; protein Mdmx; double minute 4 protein;
                     p53-binding protein Mdm4; mdm2-like p53-binding protein"
                     /codon_start=1
                     /product="protein Mdm4 isoform 2"
                     /protein_id="NP_001289732.1"
                     /db_xref="CCDS:CCDS15290.1"
                     /db_xref="GeneID:17248"
                     /db_xref="MGI:MGI:107934"
                     /translation="MTSHSTSAQCSASDSACRISSEQISQVRPKLQLLKILHAAGAQG
                     EVFTMKEVMHYLGQYIMVKQLYDQQEQHMVYCGGDLLGDLLGCQSFSVKDPSPLYDML
                     RKNLVTSASINTDAAQTLALAQDHTMDFPSQDRLKHGATEYSNPRKRTEEEDTHTLPT
                     SRHKCRDSRADEDLIEHLSQDETSRLDLDFEEWDVAGLPWWFLGNLRNNCIPKSNGST
                     DLQTNQDIGTAIVSDTTDDLWFLNETVSEQLGVGIKVEAANSEQTSEVGKTSNKKTVE
                     VGKDDDLEDSRSLSDDTDVELTSEDEWQCTECKKFNSPSKRYCFRCWALRKDWYSDCS
                     KLTHSLSTSNITAIPEKKDNEGIDVPDCRRTISAPVVRPKDGYLKEEKPRFDPCNSVG
                     FLDLAHSSESQEIISSAREQTDIFSEQKAETESMEDFQNVLKPCSLCEKRPRDGNIIH
                     GKTSHLTTCFHCARRLKKSGASCPACKKEIQLVIKVFIA"
     exon            278..352
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     exon            353..486
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     exon            487..542
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     exon            543..610
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     exon            611..710
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     exon            711..871
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     exon            872..1018
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..1099
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     exon            1100..7220
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7086..7091
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /note="hexamer: ATTAAA"
     polyA_site      7105
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /note="major polyA site"
ORIGIN      
        1 tctatggttc ccccggcctc cccggaagct cttgcgaacg ctgggtttga gaggccggaa
       61 gtggtgctgc cgttgctcgc agtttcaaaa tgcagtgcag gccttagggt ctccggctgc
      121 cacccctccc ccagctagga gggggagcga ctcatggagc ggccgtaagt ttgctaactg
      181 tggagtcttc actgccaaaa tgacatcaca ttccacctcg gcccagtgtt cagcatctga
      241 cagtgcttgc agaatttctt cggaacaaat tagtcaggtg cggccaaaac tgcagctttt
      301 gaagattttg catgcagcag gtgcgcaggg ggaagtattc accatgaaag aggtaatgca
      361 ctatctaggc cagtatataa tggtgaagca gctctatgat caacaggagc aacatatggt
      421 atactgtggt ggagatcttt tgggagatct acttggatgt cagagctttt ctgtgaaaga
      481 tccaagccct ctctatgaca tgctaagaaa gaatcttgtt acatcagctt ctattaacac
      541 agatgctgct cagactctcg ctctcgcaca ggatcacact atggattttc caagtcaaga
      601 ccgactgaag cacggtgcaa cagaatactc caatcccaga aaaagaactg aagaagagga
      661 tactcacaca ctgcctacct cacgacataa atgcagagac tccagagcag atgaagactt
      721 gatagaacat ttatctcaag atgagacatc taggcttgac cttgattttg aggagtggga
      781 cgttgctggc ctgccttggt ggtttctagg gaatttgaga aacaactgta ttcctaaaag
      841 taatggctca actgatttac agacaaatca ggatataggt actgccattg tttcagacac
      901 tacggatgat ttgtggtttt taaatgagac cgtgtcagag caattaggtg ttggaataaa
      961 agttgaagct gctaattctg agcaaacaag tgaagtaggg aaaacaagta acaagaagac
     1021 ggtggaggtg ggaaaggatg atgatcttga ggactccagg tccttgagcg atgatactga
     1081 cgtggaactt acctctgagg atgagtggca gtgtacggaa tgcaagaagt ttaattctcc
     1141 aagcaagagg tactgttttc gttgctgggc cttgagaaag gattggtatt cggattgttc
     1201 taaattaact cattccctat ctacatctaa tattactgcc atacctgaaa agaaggacaa
     1261 tgaaggaatt gatgttcccg attgtaggag aaccatttca gctcctgttg ttaggcctaa
     1321 agatggatat ttaaaggagg aaaagcccag gtttgaccct tgcaactcag tgggattttt
     1381 ggatttggct catagttctg aaagccagga gatcatctca agcgcgagag aacaaacaga
     1441 tattttttct gagcagaaag ctgaaacaga aagtatggaa gatttccaga atgtcttgaa
     1501 gccgtgtagc ttatgtgaaa aaaggcctcg ggatgggaac attattcatg ggaagacgag
     1561 ccatctgacg acatgtttcc actgtgccag gagactgaag aagtctgggg cttcgtgtcc
     1621 tgcttgtaag aaagagattc agttggttat taaagttttt atagcatagt tgagtcagtc
     1681 acagagaaat actaggagga ccaggtcatt tatcaaaaaa atcagtattc ttagaggcag
     1741 gggcagaaga tcaccaattt tgatgccagt ctgggccata taatgagatc ttagtcttaa
     1801 aaggatcagt attgagcatc ttttataaat gtgacccatt gcatatgttt atttgtataa
     1861 gcatatatga acttttagct aagttttgag ggtttcatta gtgagaagat actttgttct
     1921 tccaaattgt gaacccagag ggaataatat caatacaaac atagcaatgc attcttttat
     1981 tcacttccaa attattgcaa gaataataca ttagttgatt tattctctac cctggtgtta
     2041 tgtaatgttc ttgaggcatc aaaatctaaa gcatttatta aatactgggg ctaacactga
     2101 agaggacata tccagtcagt cgctcattgt gtagtcaagg atgaccttaa attcctgata
     2161 gctgtctcta ccacctatgt gctcataggc tcataggccg ccccactagc ccatctgaaa
     2221 tctcttagtt ttaggtcagg taacacaggt gtacatctct ccacagcctt attatcagtt
     2281 ccaaaaagga aaggcacaga ctttgttgcc cacactttgc atttgtctct caagctggct
     2341 gggtgttttg tgttttgtgc tctgcagctg ttcacattct gaggcagtgg cctatgataa
     2401 ggcttgaaca gtagagagga ggaatgtagc agctgtgaaa ggaggggaac aaattgatct
     2461 cttcaattct gcttagtcat tttagaccac atcaacttag tataatatca atatatgaac
     2521 tgaagatctt cacgctgaat cccgtatgcc tgttctgtgt ccactgaaca catggccatc
     2581 ttgatgtttc cccagggaca tgagaacatc attttaaaga ccaagtccct agaactattg
     2641 agcttgaggg caatctagct gtcttggctt ttcctatggc tcctgctgtt agaaaggggg
     2701 agatgcatgg tatccagtcc ccttaatgtc attatgtccc tgctggactg ggaccactgg
     2761 ggctcctcta attactacta agaggagcta tgttagcttt atcttgcatt gatcccatgt
     2821 tccctgtctg tcccatgctc taccattaga cccaacttta ccaaattctc actggatcag
     2881 ttatcatagt tactctttaa ttttgatgac aaagcaccaa tgcaaaatat aaaagaaagc
     2941 atttaatggg gtttgctttt gagagggtta gaatccatga tggcaggcag ggacagctga
     3001 gagctcacat cttgatctgc aagtagaatg cagagaaaaa gagagccctg gaaaacccca
     3061 gggcctgccc ccccagtgtc gtaattggtc taccttttcc accgactggg gtccaagtat
     3121 tcaaacttaa gagcctgtgg tttatattct catgtaaacc accacattcc atgaaaagaa
     3181 ttactaatta ctgtgctgca gaaccagtat taggcgttct ggacacctct tctatcaaag
     3241 aaattacttt tcactccagg cttaggcaaa ttcttaggac aagggtagaa ggcagccaca
     3301 ttctttgtca aacataacca gaataagtct ttagccagtt gttaatagtt gttctaccct
     3361 gcagcctctt gatctaggct tcctctcagt actaactgtc ttttaggctc caactggacc
     3421 cattaagctc tgtttacagc cttgaaccct ttcttagtcc aaagtcttac acattcctcc
     3481 aaaaaaacca catggtcagg tctttcacag caaagatcca cttcctggta gtagcttctg
     3541 tgtcttggct actctaaaat tgctatgaca aaacatcccg accaaggcag aatgtacaag
     3601 aaagcattga gtttatggtt tcagaggtat ttagttcatg atagaacagt tgagagctta
     3661 catcttgatc tgtaagtagg aggcagagag agaacccagg ggatggcatg gacttttgaa
     3721 acctccaagc cttcaccttg tgacagacct ccccaacaga gccacatctc ctaatccttt
     3781 ccaagtagtt cctccaactg gggaccaaat actccagcat gagcataggg gagcattctc
     3841 attcacacca ccagtttgcc gaggaacttt cctagccatg gcttgtcatt cattgctccc
     3901 agttgataat ttcactattt gtagcaaact cagtggccaa gtccttgcta ttatgggtct
     3961 tctgcttgta ttgaaaatac tgcagctcct gttggggttg ctgtgcagtc tctctgaggg
     4021 actgataaca gacttgtata tccatatctc catggcagtg agaaacggta gcttagaggt
     4081 aatcctagaa gccatcactc acagtccctg cttgagaata ggctatgata tgtctagccc
     4141 tggactttag tcatttccta gacatggacc tgctgggtgg aattgggtca ttatgatgtt
     4201 taattccaag cactgcattc aaaataggat acatgatata gtatgtcagc ttcattctaa
     4261 cgtttctcct aatcttttgg ggcagagtct tgctgtttca ccatgactgg cctgggcctt
     4321 accctacatc tcaggctggc ctcagctttg cagtgacctt cctgcttttg cctctcgagt
     4381 gctggaattc taggcatgta atgactaaat ttcttatttt gctttttatt taagtttttg
     4441 ttactctatc ttggacaaaa agtgcagagc agaggtgtcg gattactagg aaagtctcca
     4501 gggcttcgct gttactgtgt tccccacagg gacttcctgg ttgttgacgt gcttctgttt
     4561 actactgtgt cagatgagcg actcagagct cggcttcaga taggtgtgac tgccgccctc
     4621 gcccatttag gatgtagtag atttatgatt gagggctgga acgggtagat tacattgtac
     4681 cgccaagcat ttgtgactgt gtggatatat attcattatt tatgtatatt taggtgtgtg
     4741 tgatactttg catggtttga aagaatctat tctgaaagcc aggatcattt gtaacctttg
     4801 tgtgtcagtt caatgtaatg ttggggcctt tgaaaagttg attgagaaat tggggatcat
     4861 tcccagttct ccgatgttac ttgtttagtc ctctttagaa agaaagacac ttttggacca
     4921 gctgtttcgc tgttttgttc tgtttccatg atattctgtg aacctccatg gctttagata
     4981 ctgtgactgt tattttcctt tttgcccaaa ttgttgattc agtctaaaga atactatttt
     5041 ttgatgcctc ttacaattca tgcatgtgca cactatagat tgatgctacc tttgctaaaa
     5101 atgtcttaag ataaagaact agctctttag aacctgcact tcaaagataa acctggaagc
     5161 tgtcctgtgc caaaagtaaa gtgtacaatg tttacagatg ggtggaattt tgcactgcca
     5221 tagggaacgg tgagctcgca tcacggggtt ccttagatgt atgggtagat agttgcagtt
     5281 gagcttatga gaggaactgt ggagccctct aaggctaagc tgctaatgag tatccaagac
     5341 aggtggttgg tgaacgtgac tccctagacc tagctggggt cttctgttga cagctcttca
     5401 tggtttgcag acactatatg ttttctttag aggagctgcc agctctcccc tcttgtgaaa
     5461 gactgtcttg tagacatttt gagatctttt gatgtaatgt gcaatcccaa gtaatgccat
     5521 tattggtgaa tgtagcattg ttaaactttt attgtaaatt atctctgatc ttaccttgat
     5581 agccttaggg ttttttgttt ttgttttttt ttgttatgtt ttcagagaca gagtctcgct
     5641 atttggttta cctagaattt gctttatgat taggctgtcc ccaaactcac agatgtctgc
     5701 ctttctacca ccacgcccat gtaactgatg ttaccaaaaa tgatctcata tccatggtgt
     5761 tgcttatgtg tctttgcata ggcgcctcct tcagtcccat ctaggagcac agcacagtag
     5821 aagtccattt cttaagtggg cagagtcaga gataagctaa gagggcaagt agttgcttca
     5881 gtggttttta gagcttggca aacgctttac tgaagattag cattgtgttg tgacacttga
     5941 gggccaatac gagcatttaa tttgttacct aaaggtgctt tgtgttctta aacagcaagt
     6001 atttgatgtg tgtaaacatt tcccttcatt ctccctccct cctcaccgtt tttatggtct
     6061 cctatttctg agttagctgc atttggacat tgcttttatg ccagaggcca tctgtcacct
     6121 tggatgttta gaagtacagt gtacttatgt agggtcctcc ttgagcctgt cctgagcagt
     6181 gttgtggtta cagaattgcc tacgagctcc aacagagagg tggggaagct gggagggcac
     6241 agctgtctgg cttcctttgt ggaggtgttg gtttgtttga acactaagga atttgaggtt
     6301 aatcttcccc ctcttcctgc ccatagtttt cacttccctt tcagtaagaa cgacccccta
     6361 aaagaaaaca ggaggagatg cagcgcttta ggccgcctta gccagcagct tgtgtcctac
     6421 tctggaagtt gtaacctaca gtttttctca cctgtaaagc caacacacac agctacccgt
     6481 ttctacctca gtttgatgtg gcgactatgg aggaacaact cttaaaagct ctgactgcct
     6541 tgaagatgga ggaagctagt ggtcttggtc gactgtgctc tgaggtttat attgagaatc
     6601 ctttattctg gaagtgagtg cggttctcct gaccgcctgc tcagtagaac ctgtgttgcc
     6661 tgctttaagt ctctgcccac ctgcctgtcc tttccttgac actaggctct tgattaggaa
     6721 gagttgtctc cagatgcttt gagaagcaca tgtaaatcta gatagcacat tctaccctga
     6781 tggaacttcc ttgcttgtct tgctgtatag gaattcattc tgtatacact tgagtgggtg
     6841 gcatgagatg ctcgacttgt gtgtcgagag gcagtgtcat gacattccca gtctggtact
     6901 tgccagtgca gaagcaaatg aggggtaata aactgagaag aagggcagct tactaacgag
     6961 gccagaggga aatgtttccg tgtgtcctta tgtaactttt tacagtgcca ctaagttaat
     7021 agccctgtac atgggtgttt gcactatgta atgcttttaa tgcggtttta ttgtgttttt
     7081 gtgtgattaa acagttttca tttcattgtg ttttgtcatt attggaaata ggtaattttg
     7141 cttatgtaca gctaacccat tttctgttat ctttggcatg atttggggaa gtgattaaaa
     7201 gtcattggca gcttttccct
//
