LOCUS       NM_001302801            7223 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus transformed mouse 3T3 cell double minute 4 (Mdm4),
            transcript variant 1, mRNA.
ACCESSION   NM_001302801
VERSION     NM_001302801.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Yao,F., Huang,S., Liu,J., Tan,C., Xu,M., Wang,D., Huang,M., Zhu,Y.,
            Huang,X. and He,S.
  TITLE     Deletion of ARGLU1 causes global defects in alternative splicing in
            vivo and mouse cortical malformations primarily via apoptosis
  JOURNAL   Cell Death Dis 14 (8), 543 (2023)
   PUBMED   37612280
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7223)
  AUTHORS   Ueda,K.
  TITLE     Murine double minute X plays a central role in leukemic
            transformation and may be a promising target for leukemia
            prevention strategies
  JOURNAL   Exp Hematol 122, 10-18 (2023)
   PUBMED   37086813
  REMARK    GeneRIF: Murine double minute X plays a central role in leukemic
            transformation and may be a promising target for leukemia
            prevention strategies.
            Review article
REFERENCE   3  (bases 1 to 7223)
  AUTHORS   Alam,S.S., Kumar,S., Beauchamp,M.C., Bareke,E., Boucher,A.,
            Nzirorera,N., Dong,Y., Padilla,R., Zhang,S.J., Majewski,J. and
            Jerome-Majewska,L.A.
  TITLE     Snrpb is required in murine neural crest cells for proper splicing
            and craniofacial morphogenesis
  JOURNAL   Dis Model Mech 15 (6) (2022)
   PUBMED   35593225
REFERENCE   4  (bases 1 to 7223)
  AUTHORS   Bezzi,M., Teo,S.X., Muller,J., Mok,W.C., Sahu,S.K., Vardy,L.A.,
            Bonday,Z.Q. and Guccione,E.
  TITLE     Regulation of constitutive and alternative splicing by PRMT5
            reveals a role for Mdm4 pre-mRNA in sensing defects in the
            spliceosomal machinery
  JOURNAL   Genes Dev 27 (17), 1903-1916 (2013)
   PUBMED   24013503
  REMARK    GeneRIF: identify Mdm4 as one of these key mRNAs that senses the
            defects in the spliceosomal machinery and transduces the signal to
            activate the p53 response
REFERENCE   5  (bases 1 to 7223)
  AUTHORS   Wade,M., Li,Y.C. and Wahl,G.M.
  TITLE     MDM2, MDMX and p53 in oncogenesis and cancer therapy
  JOURNAL   Nat Rev Cancer 13 (2), 83-96 (2013)
   PUBMED   23303139
  REMARK    Review article
REFERENCE   6  (bases 1 to 7223)
  AUTHORS   Lenos,K. and Jochemsen,A.G.
  TITLE     Functions of MDMX in the modulation of the p53-response
  JOURNAL   J Biomed Biotechnol 2011, 876173 (2011)
   PUBMED   21541195
  REMARK    Review article
REFERENCE   7  (bases 1 to 7223)
  AUTHORS   Parant,J.M., Reinke,V., Mims,B. and Lozano,G.
  TITLE     Organization, expression, and localization of the murine mdmx gene
            and pseudogene
  JOURNAL   Gene 270 (1-2), 277-283 (2001)
   PUBMED   11404026
REFERENCE   8  (bases 1 to 7223)
  AUTHORS   Rallapalli,R., Strachan,G., Cho,B., Mercer,W.E. and Hall,D.J.
  TITLE     A novel MDMX transcript expressed in a variety of transformed cell
            lines encodes a truncated protein with potent p53 repressive
            activity
  JOURNAL   J Biol Chem 274 (12), 8299-8308 (1999)
   PUBMED   10075736
REFERENCE   9  (bases 1 to 7223)
  AUTHORS   Shvarts,A., Bazuine,M., Dekker,P., Ramos,Y.F., Steegenga,W.T.,
            Merckx,G., van Ham,R.C., van der Houven van Oordt,W., van der
            Eb,A.J. and Jochemsen,A.G.
  TITLE     Isolation and identification of the human homolog of a new
            p53-binding protein, Mdmx
  JOURNAL   Genomics 43 (1), 34-42 (1997)
   PUBMED   9226370
REFERENCE   10 (bases 1 to 7223)
  AUTHORS   Shvarts,A., Steegenga,W.T., Riteco,N., van Laar,T., Dekker,P.,
            Bazuine,M., van Ham,R.C., van der Houven van Oordt,W., Hateboer,G.,
            van der Eb,A.J. and Jochemsen,A.G.
  TITLE     MDMX: a novel p53-binding protein with some functional properties
            of MDM2
  JOURNAL   EMBO J 15 (19), 5349-5357 (1996)
   PUBMED   8895579
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CJ101093.1, AK017719.1,
            AK049576.1, AK083349.1, AK043359.1, AK046448.1 and BC058680.1.
            
            Summary: This gene encodes a protein that has been shown to
            negatively regulate the activity of the tumor suppressor protein
            p53. Homozygous knockout mice exhibit embryonic lethality as a
            result of p53-dependent apoptosis and cell cycle arrest.
            Amplification of this gene or overexpression of the encoded protein
            has been linked to a range of human cancers. A pseudogene has been
            identified on the X chromosome. Alternative splicing of this gene
            results in multiple transcript variants. [provided by RefSeq, Nov
            2014].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1). Both variants 1 and 2 encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN01164138,
                              SAMN01164142 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                CJ101093.1         1-50
            51-277              AK017719.1         1-227
            278-545             AK049576.1         216-483
            546-3483            AK083349.1         554-3491
            3484-5170           AK043359.1         910-2596
            5171-5932           AK046448.1         781-1542
            5933-7108           BC058680.1         1-1176
            7109-7223           AK046448.1         2719-2833
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 57.75 cM"
     gene            1..7223
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /note="transformed mouse 3T3 cell double minute 4"
                     /db_xref="GeneID:17248"
                     /db_xref="MGI:MGI:107934"
     exon            1..169
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     exon            170..277
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     CDS             200..1672
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein Mdm4; protein Mdmx; double minute 4 protein;
                     p53-binding protein Mdm4; mdm2-like p53-binding protein"
                     /codon_start=1
                     /product="protein Mdm4 isoform 1"
                     /protein_id="NP_001289730.1"
                     /db_xref="CCDS:CCDS78683.1"
                     /db_xref="GeneID:17248"
                     /db_xref="MGI:MGI:107934"
                     /translation="MTSHSTSAQCSASDSACRISSEQISQQVRPKLQLLKILHAAGAQ
                     GEVFTMKEVMHYLGQYIMVKQLYDQQEQHMVYCGGDLLGDLLGCQSFSVKDPSPLYDM
                     LRKNLVTSASINTDAAQTLALAQDHTMDFPSQDRLKHGATEYSNPRKRTEEEDTHTLP
                     TSRHKCRDSRADEDLIEHLSQDETSRLDLDFEEWDVAGLPWWFLGNLRNNCIPKSNGS
                     TDLQTNQDIGTAIVSDTTDDLWFLNETVSEQLGVGIKVEAANSEQTSEVGKTSNKKTV
                     EVGKDDDLEDSRSLSDDTDVELTSEDEWQCTECKKFNSPSKRYCFRCWALRKDWYSDC
                     SKLTHSLSTSNITAIPEKKDNEGIDVPDCRRTISAPVVRPKDGYLKEEKPRFDPCNSV
                     GFLDLAHSSESQEIISSAREQTDIFSEQKAETESMEDFQNVLKPCSLCEKRPRDGNII
                     HGKTSHLTTCFHCARRLKKSGASCPACKKEIQLVIKVFIA"
     exon            278..355
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     exon            356..489
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     exon            490..545
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     exon            546..613
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     exon            614..713
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     exon            714..874
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     exon            875..1021
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..1102
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     exon            1103..7223
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7089..7094
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /note="hexamer: ATTAAA"
     polyA_site      7108
                     /gene="Mdm4"
                     /gene_synonym="4933417N07Rik; Mdmx"
                     /note="major polyA site"
ORIGIN      
        1 tctatggttc ccccggcctc cccggaagct cttgcgaacg ctgggtttga gaggccggaa
       61 gtggtgctgc cgttgctcgc agtttcaaaa tgcagtgcag gccttagggt ctccggctgc
      121 cacccctccc ccagctagga gggggagcga ctcatggagc ggccgtaagt ttgctaactg
      181 tggagtcttc actgccaaaa tgacatcaca ttccacctcg gcccagtgtt cagcatctga
      241 cagtgcttgc agaatttctt cggaacaaat tagtcagcag gtgcggccaa aactgcagct
      301 tttgaagatt ttgcatgcag caggtgcgca gggggaagta ttcaccatga aagaggtaat
      361 gcactatcta ggccagtata taatggtgaa gcagctctat gatcaacagg agcaacatat
      421 ggtatactgt ggtggagatc ttttgggaga tctacttgga tgtcagagct tttctgtgaa
      481 agatccaagc cctctctatg acatgctaag aaagaatctt gttacatcag cttctattaa
      541 cacagatgct gctcagactc tcgctctcgc acaggatcac actatggatt ttccaagtca
      601 agaccgactg aagcacggtg caacagaata ctccaatccc agaaaaagaa ctgaagaaga
      661 ggatactcac acactgccta cctcacgaca taaatgcaga gactccagag cagatgaaga
      721 cttgatagaa catttatctc aagatgagac atctaggctt gaccttgatt ttgaggagtg
      781 ggacgttgct ggcctgcctt ggtggtttct agggaatttg agaaacaact gtattcctaa
      841 aagtaatggc tcaactgatt tacagacaaa tcaggatata ggtactgcca ttgtttcaga
      901 cactacggat gatttgtggt ttttaaatga gaccgtgtca gagcaattag gtgttggaat
      961 aaaagttgaa gctgctaatt ctgagcaaac aagtgaagta gggaaaacaa gtaacaagaa
     1021 gacggtggag gtgggaaagg atgatgatct tgaggactcc aggtccttga gcgatgatac
     1081 tgacgtggaa cttacctctg aggatgagtg gcagtgtacg gaatgcaaga agtttaattc
     1141 tccaagcaag aggtactgtt ttcgttgctg ggccttgaga aaggattggt attcggattg
     1201 ttctaaatta actcattccc tatctacatc taatattact gccatacctg aaaagaagga
     1261 caatgaagga attgatgttc ccgattgtag gagaaccatt tcagctcctg ttgttaggcc
     1321 taaagatgga tatttaaagg aggaaaagcc caggtttgac ccttgcaact cagtgggatt
     1381 tttggatttg gctcatagtt ctgaaagcca ggagatcatc tcaagcgcga gagaacaaac
     1441 agatattttt tctgagcaga aagctgaaac agaaagtatg gaagatttcc agaatgtctt
     1501 gaagccgtgt agcttatgtg aaaaaaggcc tcgggatggg aacattattc atgggaagac
     1561 gagccatctg acgacatgtt tccactgtgc caggagactg aagaagtctg gggcttcgtg
     1621 tcctgcttgt aagaaagaga ttcagttggt tattaaagtt tttatagcat agttgagtca
     1681 gtcacagaga aatactagga ggaccaggtc atttatcaaa aaaatcagta ttcttagagg
     1741 caggggcaga agatcaccaa ttttgatgcc agtctgggcc atataatgag atcttagtct
     1801 taaaaggatc agtattgagc atcttttata aatgtgaccc attgcatatg tttatttgta
     1861 taagcatata tgaactttta gctaagtttt gagggtttca ttagtgagaa gatactttgt
     1921 tcttccaaat tgtgaaccca gagggaataa tatcaataca aacatagcaa tgcattcttt
     1981 tattcacttc caaattattg caagaataat acattagttg atttattctc taccctggtg
     2041 ttatgtaatg ttcttgaggc atcaaaatct aaagcattta ttaaatactg gggctaacac
     2101 tgaagaggac atatccagtc agtcgctcat tgtgtagtca aggatgacct taaattcctg
     2161 atagctgtct ctaccaccta tgtgctcata ggctcatagg ccgccccact agcccatctg
     2221 aaatctctta gttttaggtc aggtaacaca ggtgtacatc tctccacagc cttattatca
     2281 gttccaaaaa ggaaaggcac agactttgtt gcccacactt tgcatttgtc tctcaagctg
     2341 gctgggtgtt ttgtgttttg tgctctgcag ctgttcacat tctgaggcag tggcctatga
     2401 taaggcttga acagtagaga ggaggaatgt agcagctgtg aaaggagggg aacaaattga
     2461 tctcttcaat tctgcttagt cattttagac cacatcaact tagtataata tcaatatatg
     2521 aactgaagat cttcacgctg aatcccgtat gcctgttctg tgtccactga acacatggcc
     2581 atcttgatgt ttccccaggg acatgagaac atcattttaa agaccaagtc cctagaacta
     2641 ttgagcttga gggcaatcta gctgtcttgg cttttcctat ggctcctgct gttagaaagg
     2701 gggagatgca tggtatccag tccccttaat gtcattatgt ccctgctgga ctgggaccac
     2761 tggggctcct ctaattacta ctaagaggag ctatgttagc tttatcttgc attgatccca
     2821 tgttccctgt ctgtcccatg ctctaccatt agacccaact ttaccaaatt ctcactggat
     2881 cagttatcat agttactctt taattttgat gacaaagcac caatgcaaaa tataaaagaa
     2941 agcatttaat ggggtttgct tttgagaggg ttagaatcca tgatggcagg cagggacagc
     3001 tgagagctca catcttgatc tgcaagtaga atgcagagaa aaagagagcc ctggaaaacc
     3061 ccagggcctg cccccccagt gtcgtaattg gtctaccttt tccaccgact ggggtccaag
     3121 tattcaaact taagagcctg tggtttatat tctcatgtaa accaccacat tccatgaaaa
     3181 gaattactaa ttactgtgct gcagaaccag tattaggcgt tctggacacc tcttctatca
     3241 aagaaattac ttttcactcc aggcttaggc aaattcttag gacaagggta gaaggcagcc
     3301 acattctttg tcaaacataa ccagaataag tctttagcca gttgttaata gttgttctac
     3361 cctgcagcct cttgatctag gcttcctctc agtactaact gtcttttagg ctccaactgg
     3421 acccattaag ctctgtttac agccttgaac cctttcttag tccaaagtct tacacattcc
     3481 tccaaaaaaa ccacatggtc aggtctttca cagcaaagat ccacttcctg gtagtagctt
     3541 ctgtgtcttg gctactctaa aattgctatg acaaaacatc ccgaccaagg cagaatgtac
     3601 aagaaagcat tgagtttatg gtttcagagg tatttagttc atgatagaac agttgagagc
     3661 ttacatcttg atctgtaagt aggaggcaga gagagaaccc aggggatggc atggactttt
     3721 gaaacctcca agccttcacc ttgtgacaga cctccccaac agagccacat ctcctaatcc
     3781 tttccaagta gttcctccaa ctggggacca aatactccag catgagcata ggggagcatt
     3841 ctcattcaca ccaccagttt gccgaggaac tttcctagcc atggcttgtc attcattgct
     3901 cccagttgat aatttcacta tttgtagcaa actcagtggc caagtccttg ctattatggg
     3961 tcttctgctt gtattgaaaa tactgcagct cctgttgggg ttgctgtgca gtctctctga
     4021 gggactgata acagacttgt atatccatat ctccatggca gtgagaaacg gtagcttaga
     4081 ggtaatccta gaagccatca ctcacagtcc ctgcttgaga ataggctatg atatgtctag
     4141 ccctggactt tagtcatttc ctagacatgg acctgctggg tggaattggg tcattatgat
     4201 gtttaattcc aagcactgca ttcaaaatag gatacatgat atagtatgtc agcttcattc
     4261 taacgtttct cctaatcttt tggggcagag tcttgctgtt tcaccatgac tggcctgggc
     4321 cttaccctac atctcaggct ggcctcagct ttgcagtgac cttcctgctt ttgcctctcg
     4381 agtgctggaa ttctaggcat gtaatgacta aatttcttat tttgcttttt atttaagttt
     4441 ttgttactct atcttggaca aaaagtgcag agcagaggtg tcggattact aggaaagtct
     4501 ccagggcttc gctgttactg tgttccccac agggacttcc tggttgttga cgtgcttctg
     4561 tttactactg tgtcagatga gcgactcaga gctcggcttc agataggtgt gactgccgcc
     4621 ctcgcccatt taggatgtag tagatttatg attgagggct ggaacgggta gattacattg
     4681 taccgccaag catttgtgac tgtgtggata tatattcatt atttatgtat atttaggtgt
     4741 gtgtgatact ttgcatggtt tgaaagaatc tattctgaaa gccaggatca tttgtaacct
     4801 ttgtgtgtca gttcaatgta atgttggggc ctttgaaaag ttgattgaga aattggggat
     4861 cattcccagt tctccgatgt tacttgttta gtcctcttta gaaagaaaga cacttttgga
     4921 ccagctgttt cgctgttttg ttctgtttcc atgatattct gtgaacctcc atggctttag
     4981 atactgtgac tgttattttc ctttttgccc aaattgttga ttcagtctaa agaatactat
     5041 tttttgatgc ctcttacaat tcatgcatgt gcacactata gattgatgct acctttgcta
     5101 aaaatgtctt aagataaaga actagctctt tagaacctgc acttcaaaga taaacctgga
     5161 agctgtcctg tgccaaaagt aaagtgtaca atgtttacag atgggtggaa ttttgcactg
     5221 ccatagggaa cggtgagctc gcatcacggg gttccttaga tgtatgggta gatagttgca
     5281 gttgagctta tgagaggaac tgtggagccc tctaaggcta agctgctaat gagtatccaa
     5341 gacaggtggt tggtgaacgt gactccctag acctagctgg ggtcttctgt tgacagctct
     5401 tcatggtttg cagacactat atgttttctt tagaggagct gccagctctc ccctcttgtg
     5461 aaagactgtc ttgtagacat tttgagatct tttgatgtaa tgtgcaatcc caagtaatgc
     5521 cattattggt gaatgtagca ttgttaaact tttattgtaa attatctctg atcttacctt
     5581 gatagcctta gggttttttg tttttgtttt tttttgttat gttttcagag acagagtctc
     5641 gctatttggt ttacctagaa tttgctttat gattaggctg tccccaaact cacagatgtc
     5701 tgcctttcta ccaccacgcc catgtaactg atgttaccaa aaatgatctc atatccatgg
     5761 tgttgcttat gtgtctttgc ataggcgcct ccttcagtcc catctaggag cacagcacag
     5821 tagaagtcca tttcttaagt gggcagagtc agagataagc taagagggca agtagttgct
     5881 tcagtggttt ttagagcttg gcaaacgctt tactgaagat tagcattgtg ttgtgacact
     5941 tgagggccaa tacgagcatt taatttgtta cctaaaggtg ctttgtgttc ttaaacagca
     6001 agtatttgat gtgtgtaaac atttcccttc attctccctc cctcctcacc gtttttatgg
     6061 tctcctattt ctgagttagc tgcatttgga cattgctttt atgccagagg ccatctgtca
     6121 ccttggatgt ttagaagtac agtgtactta tgtagggtcc tccttgagcc tgtcctgagc
     6181 agtgttgtgg ttacagaatt gcctacgagc tccaacagag aggtggggaa gctgggaggg
     6241 cacagctgtc tggcttcctt tgtggaggtg ttggtttgtt tgaacactaa ggaatttgag
     6301 gttaatcttc cccctcttcc tgcccatagt tttcacttcc ctttcagtaa gaacgacccc
     6361 ctaaaagaaa acaggaggag atgcagcgct ttaggccgcc ttagccagca gcttgtgtcc
     6421 tactctggaa gttgtaacct acagtttttc tcacctgtaa agccaacaca cacagctacc
     6481 cgtttctacc tcagtttgat gtggcgacta tggaggaaca actcttaaaa gctctgactg
     6541 ccttgaagat ggaggaagct agtggtcttg gtcgactgtg ctctgaggtt tatattgaga
     6601 atcctttatt ctggaagtga gtgcggttct cctgaccgcc tgctcagtag aacctgtgtt
     6661 gcctgcttta agtctctgcc cacctgcctg tcctttcctt gacactaggc tcttgattag
     6721 gaagagttgt ctccagatgc tttgagaagc acatgtaaat ctagatagca cattctaccc
     6781 tgatggaact tccttgcttg tcttgctgta taggaattca ttctgtatac acttgagtgg
     6841 gtggcatgag atgctcgact tgtgtgtcga gaggcagtgt catgacattc ccagtctggt
     6901 acttgccagt gcagaagcaa atgaggggta ataaactgag aagaagggca gcttactaac
     6961 gaggccagag ggaaatgttt ccgtgtgtcc ttatgtaact ttttacagtg ccactaagtt
     7021 aatagccctg tacatgggtg tttgcactat gtaatgcttt taatgcggtt ttattgtgtt
     7081 tttgtgtgat taaacagttt tcatttcatt gtgttttgtc attattggaa ataggtaatt
     7141 ttgcttatgt acagctaacc cattttctgt tatctttggc atgatttggg gaagtgatta
     7201 aaagtcattg gcagcttttc cct
//
