LOCUS       NM_010786               3124 bp    mRNA    linear   ROD 10-MAR-2024
DEFINITION  Mus musculus transformed mouse 3T3 cell double minute 2 (Mdm2),
            transcript variant 1, mRNA.
ACCESSION   NM_010786
VERSION     NM_010786.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Liu,T., Zhang,T., Guo,C., Liang,X., Wang,P. and Zheng,B.
  TITLE     Murine double minute 2-mediated estrogen receptor 1 degradation
            activates macrophage migration inhibitory factor to promote
            vascular smooth muscle cell dedifferentiation and oxidative stress
            during thoracic aortic aneurysm progression
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1871 (3), 119661 (2024)
   PUBMED   38218386
  REMARK    GeneRIF: Murine double minute 2-mediated estrogen receptor 1
            degradation activates macrophage migration inhibitory factor to
            promote vascular smooth muscle cell dedifferentiation and oxidative
            stress during thoracic aortic aneurysm progression.
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Sun,R., Zhou,Y., Liang,J., Yang,L., Fan,Z. and Wang,H.
  TITLE     Interference of MDM2 attenuates vascular endothelial dysfunction in
            hypertension partly through blocking Notch1/NLRP3 inflammasome
            pathway
  JOURNAL   Ann Anat 252, 152183 (2024)
   PUBMED   37926401
  REMARK    GeneRIF: Interference of MDM2 attenuates vascular endothelial
            dysfunction in hypertension partly through blocking Notch1/NLRP3
            inflammasome pathway.
REFERENCE   3  (bases 1 to 3124)
  AUTHORS   Ji,Q., Zhang,L. and Ye,H.
  TITLE     Melatonin improves stroke through MDM2-mediated ubiquitination of
            ACSL4
  JOURNAL   Aging (Albany NY) 16 (2), 1925-1937 (2024)
   PUBMED   38289595
  REMARK    GeneRIF: Melatonin improves stroke through MDM2-mediated
            ubiquitination of ACSL4.
REFERENCE   4  (bases 1 to 3124)
  AUTHORS   Shridhar,P., Glennon,M.S., Pal,S., Waldron,C.J., Chetkof,E.J.,
            Basak,P., Clavere,N.G., Banerjee,D., Gingras,S. and Becker,J.R.
  TITLE     MDM2 Regulation of HIF Signaling Causes Microvascular Dysfunction
            in Hypertrophic Cardiomyopathy
  JOURNAL   Circulation 148 (23), 1870-1886 (2023)
   PUBMED   37886847
  REMARK    GeneRIF: MDM2 Regulation of HIF Signaling Causes Microvascular
            Dysfunction in Hypertrophic Cardiomyopathy.
REFERENCE   5  (bases 1 to 3124)
  AUTHORS   Jin,X., Turcott,E., Englehardt,S., Mize,G.J. and Morris,D.R.
  TITLE     The two upstream open reading frames of oncogene mdm2 have
            different translational regulatory properties
  JOURNAL   J Biol Chem 278 (28), 25716-25721 (2003)
   PUBMED   12730202
REFERENCE   6  (bases 1 to 3124)
  AUTHORS   Brown,C.Y., Mize,G.J., Pineda,M., George,D.L. and Morris,D.R.
  TITLE     Role of two upstream open reading frames in the translational
            control of oncogene mdm2
  JOURNAL   Oncogene 18 (41), 5631-5637 (1999)
   PUBMED   10523842
REFERENCE   7  (bases 1 to 3124)
  AUTHORS   Oliner,J.D., Kinzler,K.W., Meltzer,P.S., George,D.L. and
            Vogelstein,B.
  TITLE     Amplification of a gene encoding a p53-associated protein in human
            sarcomas
  JOURNAL   Nature 358 (6381), 80-83 (1992)
   PUBMED   1614537
REFERENCE   8  (bases 1 to 3124)
  AUTHORS   Momand,J., Zambetti,G.P., Olson,D.C., George,D. and Levine,A.J.
  TITLE     The mdm-2 oncogene product forms a complex with the p53 protein and
            inhibits p53-mediated transactivation
  JOURNAL   Cell 69 (7), 1237-1245 (1992)
   PUBMED   1535557
REFERENCE   9  (bases 1 to 3124)
  AUTHORS   Donehower,L.A., Harvey,M., Slagle,B.L., McArthur,M.J.,
            Montgomery,C.A. Jr., Butel,J.S. and Bradley,A.
  TITLE     Mice deficient for p53 are developmentally normal but susceptible
            to spontaneous tumours
  JOURNAL   Nature 356 (6366), 215-221 (1992)
   PUBMED   1552940
REFERENCE   10 (bases 1 to 3124)
  AUTHORS   Fakharzadeh,S.S., Trusko,S.P. and George,D.L.
  TITLE     Tumorigenic potential associated with enhanced expression of a gene
            that is amplified in a mouse tumor cell line
  JOURNAL   EMBO J 10 (6), 1565-1569 (1991)
   PUBMED   2026149
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY323121.1, AK004719.1 and BP767471.1.
            
            [WARNING] On Mar 18, 2024 this sequence was replaced by
            NM_010786.5.
            
            On Jan 3, 2014 this sequence version replaced NM_010786.3.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC050902.1, AK004719.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            regulatory uORF        :: PMID: 12730202
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-42                BY323121.1         2-43
            43-3069             AK004719.1         2-3028
            3070-3124           BP767471.1         23-77               c
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 66.32 cM"
     gene            1..3124
                     /gene="Mdm2"
                     /gene_synonym="1700007J15Rik; Mdm-2"
                     /note="transformed mouse 3T3 cell double minute 2"
                     /db_xref="GeneID:17246"
                     /db_xref="MGI:MGI:96952"
     exon            1..207
                     /gene="Mdm2"
                     /gene_synonym="1700007J15Rik; Mdm-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      134..178
                     /regulatory_class="uORF"
                     /gene="Mdm2"
                     /gene_synonym="1700007J15Rik; Mdm-2"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:12730202]"
                     /note="this uORF is predicted to enocde a 14 aa peptide
                     (PMID:12730202)"
     regulatory      190..204
                     /regulatory_class="uORF"
                     /gene="Mdm2"
                     /gene_synonym="1700007J15Rik; Mdm-2"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:12730202]"
                     /note="this uORF is predicted to enocde a 4 aa peptide
                     (PMID:12730202)"
     exon            208..290
                     /gene="Mdm2"
                     /gene_synonym="1700007J15Rik; Mdm-2"
                     /inference="alignment:Splign:2.1.0"
     exon            291..375
                     /gene="Mdm2"
                     /gene_synonym="1700007J15Rik; Mdm-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             295..1764
                     /gene="Mdm2"
                     /gene_synonym="1700007J15Rik; Mdm-2"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1; E3
                     ubiquitin-protein ligase Mdm2; oncoprotein Mdm2; double
                     minute 2 protein; p53-binding protein Mdm2; RING-type E3
                     ubiquitin transferase Mdm2"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase Mdm2 isoform 1"
                     /protein_id="NP_034916.1"
                     /db_xref="CCDS:CCDS24194.1"
                     /db_xref="GeneID:17246"
                     /db_xref="MGI:MGI:96952"
                     /translation="MCNTNMSVSTEGAASTSQIPASEQETLVRPKPLLLKLLKSVGAQ
                     NDTYTMKEIIFYIGQYIMTKRLYDEKQQHIVYCSNDLLGDVFGVPSFSVKEHRKIYAM
                     IYRNLVAVSQQDSGTSLSESRRQPEGGSDLKDPLQAPPEEKPSSSDLISRLSTSSRRR
                     SISETEENTDELPGERHRKRRRSLSFDPSLGLCELREMCSGGSSSSSSSSSESTETPS
                     HQDLDDGVSEHSGDCLDQDSVSDQFSVEFEVESLDSEDYSLSDEGHELSDEDDEVYRV
                     TVYQTGESDTDSFEGDPEISLADYWKCTSCNEMNPPLPSHCKRCWTLRENWLPDDKGK
                     DKVEISEKAKLENSAQAEEGLDVPDGKKLTENDAKEPCAEEDSEEKAEQTPLSQESDD
                     YSQPSTSSSIVYSSQESVKELKEETQDKDESVESSFSLNAIEPCVICQGRPKNGCIVH
                     GKTGHLMSCFTCAKKLKKRNKPCPVCRQPIQMIVLTYFN"
     exon            376..450
                     /gene="Mdm2"
                     /gene_synonym="1700007J15Rik; Mdm-2"
                     /inference="alignment:Splign:2.1.0"
     exon            451..584
                     /gene="Mdm2"
                     /gene_synonym="1700007J15Rik; Mdm-2"
                     /inference="alignment:Splign:2.1.0"
     exon            585..634
                     /gene="Mdm2"
                     /gene_synonym="1700007J15Rik; Mdm-2"
                     /inference="alignment:Splign:2.1.0"
     exon            635..693
                     /gene="Mdm2"
                     /gene_synonym="1700007J15Rik; Mdm-2"
                     /inference="alignment:Splign:2.1.0"
     exon            694..790
                     /gene="Mdm2"
                     /gene_synonym="1700007J15Rik; Mdm-2"
                     /inference="alignment:Splign:2.1.0"
     exon            791..954
                     /gene="Mdm2"
                     /gene_synonym="1700007J15Rik; Mdm-2"
                     /inference="alignment:Splign:2.1.0"
     exon            955..1110
                     /gene="Mdm2"
                     /gene_synonym="1700007J15Rik; Mdm-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..1188
                     /gene="Mdm2"
                     /gene_synonym="1700007J15Rik; Mdm-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1189..3099
                     /gene="Mdm2"
                     /gene_synonym="1700007J15Rik; Mdm-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3054..3059
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mdm2"
                     /gene_synonym="1700007J15Rik; Mdm-2"
     polyA_site      3098
                     /gene="Mdm2"
                     /gene_synonym="1700007J15Rik; Mdm-2"
ORIGIN      
        1 gttctctccg cggacggtag ggggcgctcg tcacagaact ctgctttgtt aacggggcct
       61 ccggggccag cgtagcctag gagcggccgg tgaggagccg ccgccttctc gtcgctcgag
      121 ctctggagcg accatggtcg ctcaggcccc ggccgcgggg cctccgcgct ccccgtgaag
      181 ggtcggaaga tgcgcgggaa gtagcagccg tctgctgggc gagcgggaga ccgaccggac
      241 acccctgggg gaccctctcg gatcaccgcg cttctcctgc gggcctccag gccaatgtgc
      301 aataccaaca tgtctgtgtc taccgagggt gctgcaagca cctcacagat tccagcttcg
      361 gaacaagaga ctctggttag accaaaacca ttgcttttga agttgttaaa gtccgttgga
      421 gcgcaaaacg acacttacac tatgaaagag attatatttt atattggcca gtatattatg
      481 actaagaggt tatatgacga gaagcagcag cacattgtgt attgttcaaa tgatctccta
      541 ggagatgtgt ttggagtccc gagtttctct gtgaaggagc acaggaaaat atatgcaatg
      601 atctacagaa atttagtggc tgtaagtcag caagactctg gcacatcgct gagtgagagc
      661 agacgtcagc ctgaaggtgg gagtgatctg aaggatcctt tgcaagcgcc accagaagag
      721 aaaccttcat cttctgattt aatttctaga ctgtctacct catctagaag gagatccatt
      781 agtgagacag aagagaacac agatgagcta cctggggagc ggcaccggaa gcgccgcagg
      841 tccctgtcct ttgatccgag cctgggtctg tgtgagctga gggagatgtg cagcggcggc
      901 agcagcagca gtagcagcag cagcagcgag tccacagaga cgccctcgca tcaggatctt
      961 gacgatggcg taagtgagca ttctggtgat tgcctggatc aggattcagt ttctgatcag
     1021 tttagcgtgg aatttgaagt tgagtctctg gactcggaag attacagcct gagtgacgaa
     1081 gggcacgagc tctcagatga ggatgatgag gtctatcggg tcacagtcta tcagacagga
     1141 gaaagcgata cagactcttt tgaaggagat cctgagattt ccttagctga ctattggaag
     1201 tgtacctcat gcaatgaaat gaatcctccc cttccatcac actgcaaaag atgctggacc
     1261 cttcgtgaga actggcttcc agacgataag gggaaagata aagtggaaat ctctgaaaaa
     1321 gccaaactgg aaaactcagc tcaggcagaa gaaggcttgg atgtgcctga tggcaaaaag
     1381 ctgacagaga atgatgctaa agagccatgt gctgaggagg acagcgagga gaaggccgaa
     1441 cagacgcccc tgtcccagga gagtgacgac tattcccaac catcgacttc cagcagcatt
     1501 gtttatagca gccaagaaag cgtgaaagag ttgaaggagg aaacgcagga caaagacgag
     1561 agtgtggaat ctagcttctc cctgaatgcc atcgaaccat gtgtgatctg ccaggggcgg
     1621 cctaaaaatg gctgcattgt tcacggcaag actggacacc tcatgtcatg tttcacgtgt
     1681 gcaaagaagc taaaaaaaag aaacaagccc tgcccagtgt gcagacagcc aatccaaatg
     1741 attgtgctaa cttacttcaa ctagctgacc tgctcacaaa aatagaattt tatatttcta
     1801 actatatgac ccccaaatta gacaacatgg gtattatttt tatacattaa agccagaaaa
     1861 ctgtcttagt ccacataaaa ttcacttata atttatcctg gagagtaaat atggtgaata
     1921 ttttcttcct ttttagggaa atttcacttg tttattttat atttgtgttt taagtaattt
     1981 gcattggctg tttatatttt ccttatattt taaataatct ccgcttggaa ggactttgga
     2041 agtgtatgtg agaagtcctt tccatctcct gcagatgatg gtggaccttc ctcatcaagg
     2101 gctacagaag tacttgattt ctgttttttt tgttaataat aagaacattt aatttattta
     2161 gtgtctttca tgtaaagagt taaagactat gtgaaggatt gtatatttaa gttattgaaa
     2221 ttctgaaact gtagtaatct aaaatgtgtg agttgtgggc tgcagagaag actcagccag
     2281 taaaggcacc tgctgtgtac acccactgac acacatttga tccttggaac ccccaggaag
     2341 agtgaaccag ttccacaaag ttgttccctg atcttcaaat ggatgcacgc atgcacgcac
     2401 acacacgcgc gcgtgcgcac acacacacac acacacacac acacacacac acacacacac
     2461 acacacacag ttttaaaggc atgaattgca tctggtggta tgtaagtgaa aacacacgcc
     2521 ttattttcca gcattttcag ctttttgtca taggggtgtg gcacaagtgt tgcagtttgt
     2581 cccaggttga aaagcctgag gctggtagaa gcgccttttt gcctcagctc cgtggttcct
     2641 ggtggttgcc tatgtttcag gcctgtactt aggctaggtt tagaaaccag cccattcaga
     2701 aagactgaat cagaacatgg ataaagtgaa ctcattctaa gatgactcgt ctatccatgt
     2761 agattaatct cctggttcat aataggcctc ttccctttga ttgaagggtc acgtctaagt
     2821 atagaaaaca taaaactgta aggtagagga agcgaaggat agctttgtat taatgttgcg
     2881 ttaaagcttc agagacaaga acaagaacac tcctcccacg tgacagcatt tgaataggag
     2941 gcggtgggtg cggcagcctg ggcagcttca gtcccgattt acaataaagt accttgtgtg
     3001 ttattagttc ttaaatgttt atttagaaat ggcattgatg ttatttattt gcaaataaat
     3061 ggtttattga agaattgtga aagagatttg tcttacacaa aaaaaaaaaa aaaaaaaaaa
     3121 aaaa
//
