LOCUS       NM_207010               9813 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Mus musculus MAM domain containing glycosylphosphatidylinositol
            anchor 2 (Mdga2), transcript variant B, mRNA.
ACCESSION   NM_207010 XM_138145
VERSION     NM_207010.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9813)
  AUTHORS   Fertan,E., Wong,A.A., Montbrun,T.S.G., Purdon,M.K., Roddick,K.M.,
            Yamamoto,T. and Brown,R.E.
  TITLE     Early postnatal development of the MDGA2+/- mouse model of synaptic
            dysfunction
  JOURNAL   Behav Brain Res 452, 114590 (2023)
   PUBMED   37499910
  REMARK    GeneRIF: Early postnatal development of the MDGA2[+/-] mouse model
            of synaptic dysfunction.
REFERENCE   2  (bases 1 to 9813)
  AUTHORS   Chen,P., Liu,Z., Zhang,Q., Lin,D., Song,L., Liu,J., Jiao,H.F.,
            Lai,X., Zou,S., Wang,S., Zhou,T., Li,B.M., Zhu,L., Pan,B.X. and
            Fei,E.
  TITLE     DSCAM Deficiency Leads to Premature Spine Maturation and
            Autism-like Behaviors
  JOURNAL   J Neurosci 42 (4), 532-551 (2022)
   PUBMED   34848499
REFERENCE   3  (bases 1 to 9813)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9813)
  AUTHORS   Gautam,M., Xie,E.F., Kocak,N. and Ozdinler,P.H.
  TITLE     Mitoautophagy: A Unique Self-Destructive Path Mitochondria of Upper
            Motor Neurons With TDP-43 Pathology Take, Very Early in ALS
  JOURNAL   Front Cell Neurosci 13, 489 (2019)
   PUBMED   31787882
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9813)
  AUTHORS   Barham,C., Fil,D., Byrum,S.D., Rahmatallah,Y., Glazko,G. and
            Kiaei,M.
  TITLE     RNA-Seq Analysis of Spinal Cord Tissues from hPFN1G118V Transgenic
            Mouse Model of ALS at Pre-symptomatic and End-Stages of Disease
  JOURNAL   Sci Rep 8 (1), 13737 (2018)
   PUBMED   30213953
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9813)
  AUTHORS   Pettem,K.L., Yokomaku,D., Takahashi,H., Ge,Y. and Craig,A.M.
  TITLE     Interaction between autism-linked MDGAs and neuroligins suppresses
            inhibitory synapse development
  JOURNAL   J Cell Biol 200 (3), 321-336 (2013)
   PUBMED   23358245
  REMARK    GeneRIF: MDGA binding and suppression of synaptogenic activity was
            selective for neuroligin-2 and not neuroligin-1 excitatory synapse
            organizer.
REFERENCE   7  (bases 1 to 9813)
  AUTHORS   Suzuki,M., Solter,D. and Watanabe,T.
  TITLE     Transgene insertion in intronic sequences of Mdga2 gene shows
            methylation in an imprinted manner in an Acrodysplasia (Adp) mouse
            line
  JOURNAL   Biochem Biophys Res Commun 418 (3), 439-444 (2012)
   PUBMED   22281501
  REMARK    GeneRIF: The transgene was inserted into the intronic sequences
            between exon 3 and exon 4 of the Mdga2 gene;the transgene was
            highly or under methylated in normal and deformed mice,
            respectively.
REFERENCE   8  (bases 1 to 9813)
  AUTHORS   Watanabe,T., Tarttelin,E., Neubuser,A., Kimura,M. and Solter,D.
  TITLE     Fine genetic mapping defines the genetic order of Pax9, Tcf3a, and
            Acrodysplasia (Adp)
  JOURNAL   Mamm Genome 5 (12), 768-770 (1994)
   PUBMED   7894157
REFERENCE   9  (bases 1 to 9813)
  AUTHORS   Boulter,C.A., Aguzzi,A., Evans,M.J. and Affara,N.
  TITLE     A chimaeric mouse model for autosomal-dominant polycystic kidney
            disease
  JOURNAL   Contrib Nephrol 97, 60-70 (1992)
   PUBMED   1633717
REFERENCE   10 (bases 1 to 9813)
  AUTHORS   DeLoia,J.A. and Solter,D.
  TITLE     A transgene insertional mutation at an imprinted locus in the mouse
            genome
  JOURNAL   Dev Suppl, 73-79 (1990)
   PUBMED   2090433
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC117250.3, AC125379.4, AC140249.2 and AC164164.3.
            
            On Jul 20, 2010 this sequence version replaced NM_207010.1.
            
            Transcript Variant: This variant (B) lacks an in-frame exon in the
            coding region, compared to variant A. The resulting protein
            (isoform B) is shorter, compared to isoform A.
            
            Sequence Note: This RefSeq record was created from genomic sequence
            data to make the sequence consistent with the reference genome
            assembly. The genomic coordinates used for the transcript record
            were based on transcript alignments.
            
            CCDS Note: The coding region has been updated to annotate a
            downstream in-frame AUG. The downstream AUG is predicted to encode
            a signal peptide which is expected for this GPI-anchored protein.
            PMID: 23358245  shows evidence that this protein is processed
            through the ER and would likely require a signal peptide. The
            upstream in-frame AUG is likely bypassed by leaky scanning to allow
            for translation from the downstream AUG.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY371925.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849379,
                                           SAMN00849381 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: downstream AUG is associated
                                                with N-terminal localization
                                                signal (PMID:23358245)
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1580              AC117250.3         77224-78803         c
            1581-1720           AC125379.4         92035-92174
            1721-1895           AC125379.4         163039-163213
            1896-2092           AC140249.2         25173-25369
            2093-2225           AC140249.2         31567-31699
            2226-2495           AC140249.2         58775-59044
            2496-2825           AC164164.3         241947-242276       c
            2826-3119           AC164164.3         216744-217037       c
            3120-3389           AC164164.3         155582-155851       c
            3390-3538           AC164164.3         137320-137468       c
            3539-3716           AC164164.3         100021-100198       c
            3717-3872           AC164164.3         93506-93661         c
            3873-4031           AC164164.3         92945-93103         c
            4032-4162           AC164164.3         73550-73680         c
            4163-4268           AC164164.3         60041-60146         c
            4269-9813           AC164164.3         52898-58442         c
FEATURES             Location/Qualifiers
     source          1..9813
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 27.63 cM"
     gene            1..9813
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /note="MAM domain containing glycosylphosphatidylinositol
                     anchor 2"
                     /db_xref="GeneID:320772"
                     /db_xref="MGI:MGI:2444706"
     exon            1..1580
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /inference="alignment:Splign:2.1.0"
     CDS             1508..4357
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /note="isoform B precursor is encoded by transcript
                     variant B; MAM domain containing 1; MAM domain-containing
                     glycosylphosphatidylinositol anchor protein 2;
                     acrodysplasia"
                     /codon_start=1
                     /product="MAM domain-containing
                     glycosylphosphatidylinositol anchor protein 2 isoform B
                     precursor"
                     /protein_id="NP_996893.3"
                     /db_xref="CCDS:CCDS25945.1"
                     /db_xref="GeneID:320772"
                     /db_xref="MGI:MGI:2444706"
                     /translation="MDLVYGLVWLLTVLLEGISGQGVYAPPTVRIVHSGLACNIEEER
                     YSERVYTIREGETLELTCLVTGHPRPQIRWTKTAGSASDRFQDSSVFNETLRITNIQR
                     HQGGRYYCKAENGLGSPAIKSIRVDVYYLDDPVVTVHQSIGEAKEQFYYERTVFLRCV
                     ANSNPPVRYSWRRGQEVLLQGSDKGVEIYEPFFTQGETKILKLKNLRPQDYANYSCIA
                     SVRNVCNIPDKMVSFRLSNKTASPSIKLLVDDPIVVNPGEAITLVCVTTGGEPTPSLT
                     WVRSFGTLPEKIVLNGGTLTIPAITSDDAGTYSCIANNNVGNPAKKSTNIIVRALKKG
                     RFWITPDPYHKDDNIQIGREVKISCQVEAVPSEELTFSWFKNGRPLRSSERMVITQTD
                     PDVSPGTTNLDIIDLKFTDFGTYTCVASLKGGGISDISIDVNISSSTVPPNLTVPQEK
                     SPLVTREGDTIELQCQVTGKPKPIILWSRADKEVAMPDGTMQMESYDGTLRIVNVSRE
                     MSGMYRCQTSQYNGFNVKPREALVQLIVQYPPAVEPAFLEIRQGQDRSVTMSCRVLRA
                     YPIRVLTYEWRLGNKLLRTGQFDSQEYTEYPLKSLSNENYGVYNCSIINEAGAGRCSF
                     LVTGKAYAPEFYYDTYNPVWQNRHRVYSYSLQWTQMNPDAVDRIVAYRLGIRQAGQQR
                     WWEQEIKINGNIQKGELITYNLTELIKPEAYEVRLTPLTKFGEGDSTIRVIKYTGEFH
                     CGFEDGNICLFTQDDTDNFDWTKQSTATRNTKYTPNTGPSADRSGSKEGFYMYIETSR
                     PRLEGEKARLLSPVFSIAPKNPYGPTNSAYCFSFFYHMYGQHIGVLNVYLRLKGQTTI
                     ENPLWSSSGNKGQRWNEAHVNIYPITSFQLIFEGIRGPGIEGDIAIDDVSIAEGECAK
                     QDLPTKNSVDGAVGILVHIWLFPVIILISILSPRR"
     exon            1581..1720
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /inference="alignment:Splign:2.1.0"
     exon            1721..1895
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /inference="alignment:Splign:2.1.0"
     exon            1896..2092
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /inference="alignment:Splign:2.1.0"
     exon            2093..2225
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /inference="alignment:Splign:2.1.0"
     exon            2226..2495
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /inference="alignment:Splign:2.1.0"
     exon            2496..2825
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /inference="alignment:Splign:2.1.0"
     exon            2826..3119
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /inference="alignment:Splign:2.1.0"
     exon            3120..3389
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /inference="alignment:Splign:2.1.0"
     exon            3390..3538
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /inference="alignment:Splign:2.1.0"
     exon            3539..3716
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /inference="alignment:Splign:2.1.0"
     exon            3717..3872
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /inference="alignment:Splign:2.1.0"
     exon            3873..4031
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /inference="alignment:Splign:2.1.0"
     exon            4032..4162
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /inference="alignment:Splign:2.1.0"
     exon            4163..4268
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /inference="alignment:Splign:2.1.0"
     exon            4269..9813
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6285..6290
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /note="hexamer: AATAAA"
     polyA_site      6316
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /note="major polyA site"
     regulatory      8492..8497
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /note="hexamer: AATAAA"
     polyA_site      8514
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
     regulatory      9504..9509
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
                     /note="hexamer: AATAAA"
     polyA_site      9538
                     /gene="Mdga2"
                     /gene_synonym="6720489L24Rik; 9330209L04Rik; Adp; Mamdc1;
                     Tg(Prnp-PFN1*G118V)838Kiaei"
ORIGIN      
        1 ccccggcagc ctggccgcct gcgctccgcg tccgccgctt ctccatcctc ctcctcttct
       61 tcctcctcct cctccttcct atcgcctccg cggtgcgcaa cccccagcgt caatccaggg
      121 atcccgagca agcgtagggc cccggacgct cctctgccca gcttgggcat ctctgccgca
      181 actgcggccc gcaaggcggc cgcgcagccg gggtgccctc agcaggcggc cgcttctctg
      241 ttccgctccg gatgccagca ctccgttagc ccgggtgggg aaagcccttc tgcggctccc
      301 gggtctaaac tcccgccagt ctccaccgcc cccaccccct ccacctggcg ggggcggcgc
      361 ggaagcgaag aggactcgcg gtacgggtgg gcacgcgtgg acggtgtgcg gatctgctct
      421 gcgctcggca tcctccctct cctgcaagtg gtggtgctcg gactctgctt ttggctccgg
      481 agattggtgc tgcctccagg agcaccctct ttgctggcgg ggcgctcaag gctgtttgga
      541 agagccgtgg cggtggcggc agcggctgag gctggtgcct ggcgagctcc agacgggcgc
      601 tccgctcccg cttggaccct ttcgccctcc tctctcctcc tccgctccct cctccccatc
      661 ccccgtttcc cactcctcct cttcctcctc ctcctcttcc tctccccgct cggcgatgcg
      721 agtctctgtc gtgtaacagg attggcgttg caatggcaac tgatggaatg ggggaaggca
      781 agacagcagg gctgggggct ccggactgcg ggcgggcgag ccgctgccgc cgcttgacgc
      841 tcctctgggg accttcgcga gttactttgt aaaggcgaac ccgggcgagg gagccccgct
      901 tcgtgcccgg gtcagaggcc acttcctgct ccctggctgt ccagcgcggt tcgcgtcgcg
      961 cggcgccccc agggctcctg cccgctcctg ccgcttgccc gcgcccggag cccggagccg
     1021 cttccctgaa gaacacttgt cctcacagct ctgcttccca tcaccgcccc ctgctcctcc
     1081 ccggcgtctg cgattttccc aggcgcccgg tgtgagtgcg gcttgtgtgt gagtgcatgt
     1141 attggggtat tgtgtagtgg gagtgtgttc ccgcgtgtgt gtgcttatac gtgtgtgctt
     1201 gtgcgtgtgt gtgtgtgtgt gtgtgtgagc gtgtgtgtga gcgtgtgtgt gcgtgtgtgt
     1261 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgc atgagtgtgg ggtgtgtggg
     1321 gctccagcgc tccgctcacg gccgccgcca gggaaggaca gacggacagt gcttcctccc
     1381 attgctagca gttcccgggc acctcgactc ggcccgggca caagtggagc gcgccttgct
     1441 ggccgctggc ctccagaagg tcccactgcg tcccccttgg gctggatatg ttcatgttca
     1501 cgtgaagatg gatttagtgt acggtctcgt gtggctgctg acagtcctcc tggagggaat
     1561 ctctggccaa ggagtgtacg ctcccccgac agttcggatt gtgcactcag gattggcctg
     1621 taatatcgag gaggagcgct actccgaaag ggtctatacc atccgggaag gagaaactct
     1681 agaattgacc tgcctggtta ctggacatcc acgcccacag atcaggtgga ccaaaacagc
     1741 aggaagcgcc tctgacagat tccaagactc aagtgtcttc aatgagactt tgaggattac
     1801 aaacattcag agacaccaag gaggccgata ttactgtaaa gcagagaatg gcttggggtc
     1861 accagcaata aagtcgatcc gagtggatgt gtactacttg gatgatccag tagtaactgt
     1921 tcatcaaagt ataggtgaag ctaaagaaca attttactat gagagaacag tgtttctccg
     1981 atgtgtggcc aattccaacc cccctgtgcg ctatagctgg agacgtggtc aggaggtatt
     2041 actacaagga tctgacaagg gagtggagat ttatgagccc ttttttaccc agggggaaac
     2101 gaagatctta aagctaaaga atcttcgccc tcaagattat gctaactaca gctgcattgc
     2161 ttcagtaagg aacgtgtgta atattccaga caagatggtg tcgtttagac tttccaataa
     2221 aactgcttct ccgtcaatta agctcttggt ggatgatcct atagttgtaa atcctggaga
     2281 ggccataacg ttagtatgtg ttacaactgg aggagagcct acaccctctc ttacctgggt
     2341 caggtccttc gggactctgc ctgaaaagat tgttttgaat ggagggacat tgaccatacc
     2401 tgccatcacc tctgatgacg ctggtactta cagctgcatt gccaataata atgtgggaaa
     2461 ccctgcaaaa aagtccacca acatcattgt gagagcatta aaaaaaggac gattttggat
     2521 cactcctgat ccttatcaca aagatgacaa catccagatt gggcgtgagg tgaaaatatc
     2581 ttgccaagta gaagctgttc cctctgagga gttaacattc agttggttta aaaatggtcg
     2641 tccattaaga agttctgaac ggatggttat tacacagact gaccctgatg tgtcccccgg
     2701 aacaacaaac ttggatatca ttgacttaaa attcacagac tttgggactt acacatgcgt
     2761 ggcctctctg aagggaggag gaatatctga tatcagtatt gatgttaaca tttccagcag
     2821 tacagttcca cccaatctga ctgttccaca ggaaaaatca cctttggtca ccagagaagg
     2881 agacacaata gaacttcagt gtcaagtaac tgggaaacca aaaccaatca tcctttggtc
     2941 tagagcagat aaagaagttg caatgcctga tgggacaatg caaatggaaa gttatgatgg
     3001 gaccctgagg attgtgaatg tttcaaggga aatgtcagga atgtacagat gccagaccag
     3061 ccagtacaat ggattcaacg tgaagccaag agaagccttg gtgcagctca ttgtgcagta
     3121 tcccccagct gtggaaccag cattcttgga aatccgtcaa ggacaggaca gaagtgtgac
     3181 catgagctgt agggtcctga gagcctatcc aatacgagtg ctgacctatg agtggcgtct
     3241 gggcaataaa ttattacgga ccggacagtt tgactctcag gagtatacag agtacccact
     3301 aaagagtctt tccaatgaaa actatggagt ttataactgc agcatcataa atgaagctgg
     3361 agcagggaga tgcagctttc ttgttacagg aaaggcctat gccccagagt tctactatga
     3421 cacctacaac cccgtgtggc agaaccgaca ccgagtgtat tcttacagtc tacagtggac
     3481 acaaatgaac cctgatgcag ttgatcgaat tgttgcatac cgcttgggta ttcggcaggc
     3541 tggacagcaa cgctggtggg aacaggagat taaaataaat ggaaatattc agaaaggaga
     3601 actgattaca tataacttga ctgaacttat taaaccagag gcctatgaag tccgattgac
     3661 tcctctcact aaatttggtg aaggagattc aacaattcgt gtaattaaat atacagggga
     3721 atttcactgt ggatttgaag atggtaatat ttgtttgttc acccaagacg atacggataa
     3781 ctttgactgg acaaagcaaa gcactgcaac aaggaacaca aaatacaccc ctaacacggg
     3841 tcccagtgca gatcggagtg gttccaaaga aggtttttat atgtacattg agacatcgcg
     3901 acccagacta gaaggcgaaa aggctcgact tctcagccct gtattcagca tagctcccaa
     3961 aaatccatat ggacctacaa atagtgcata ttgtttcagt ttcttctatc acatgtacgg
     4021 gcaacatata ggggttttaa atgtatatct acgtttgaaa gggcagacaa cgatagagaa
     4081 tccgctatgg tcgtcgagtg ggaacaaagg acaacgatgg aatgaagctc atgttaatat
     4141 atatccaatt acttcatttc agttaatttt tgaaggcatt cgaggtcctg ggatagaggg
     4201 tgacatcgcc attgatgatg tatcaattgc tgaaggagaa tgtgcaaaac aagacctacc
     4261 aactaagaat tccgtggatg gtgctgttgg gatcttagtt catatatggc tttttccagt
     4321 tatcatcctc atctctatct taagccctcg aaggtgacct tatcctggca gaggctataa
     4381 atgattcacc aggcactggc atgaagaaag agtctttgta aatggacact gaaaaaacaa
     4441 actaccaaag attcctccac tgactactga ctcaaaaatt aaataataaa aacaacattt
     4501 ttttaagcac tggggattaa aaaaaaagac atcatggaaa tataacttat tccaaactac
     4561 atatagaaga tattcttggc ctgggtacaa aagagccctt caggtgcttt tgtggctaaa
     4621 atgattacag cttcatctgg ttgaactctg gaaaaagaaa ataaaagaaa aagaaagaat
     4681 gagagaaaaa gaagaagaaa aagagctata gaaatcctcg tcaaagcaca aagtcatggc
     4741 tggttttgtt tcaaatgaat agtttgcttg ttaccatgga aacctaatgg cctgccaaca
     4801 aaaacctcac tgtaaacagg gtacgtggag agctggcatt tattttcctt tcaagaaggt
     4861 tttatgtaga gaattaaata aatgtaggcc cttttacctt tggctgttac ccttccttga
     4921 aaatactcca aactcggaat tatttaaagg tttcatatcc ctccccgcct tatcccaccc
     4981 cacttttact gttgtttggg ttttgttctt gttgttcttg ttgttcttgt tgttgttact
     5041 gtttgttgta gttcttttct ttttcctttt ttctttcttt tttctccctc acatggctaa
     5101 gctagataac tgtatgatgt ctctttttgc atgcactact atccaggatg gtaaacctgg
     5161 gcttacatat tacacaggct tatcggagtt tgcttgcggc cacttgacac ccaaactacg
     5221 tcatctgttc ctatgaagaa accaaaccac catcttttat aaattgccat ggaaaccaag
     5281 tgccttcaat gaaacaagcc tgaataattt tcaactcaga agcttgcact gctaacaatg
     5341 gtttgtgtaa aactattttg taaacagacg acacagccta aatgtgcaaa ttacaggcaa
     5401 gacaccctag ccgcttctga agaagaaagg acccacgctg ctgcgtaaac ccatgcagat
     5461 tatgtgtttc acctcctaga cagccatggc cttttggctt aatgtccata gaaagtaact
     5521 tccattgaga ccagacatgg gagcaacact tttttcttcc aggttattaa atgggtcaac
     5581 cagagcaaaa ggttgttgag ataatactta gcgcagaagg tggtaaaaca cagaagtgat
     5641 ttttttttac tctaacctcc atttcaaatg agattggctt tgcctttaga gggaacgagg
     5701 aaatgtttga gattgccgtg catacaaact ctgccgcgga actgggcctg aaaacaatct
     5761 ggctttattc taaacactga gggtaatatg cctctgccct ttagtcagac tctgtgcaaa
     5821 ttgtgaaata ttattttatt attttaacaa gattaaaaaa aacactttta caaaaaaaca
     5881 aaaacctgta aaaatgattt tgagctacct gaggacaaac cataacttta accagccagt
     5941 tttccaatgc attgtaaatt tcacttaaac ctgttggtta tgacaatttg aagatctttt
     6001 tccttaaatt atctcagctt ttaacttcat ttaaaaagac ttttgtctaa agaagaatat
     6061 tattattatc tgttctttca acaccccagg atttaaaaaa aaattactga ttatgcaagt
     6121 aattgggatc taagtattaa attgcagcat ttgcaaatat taatataaaa agcacaacaa
     6181 aaatgtagtg gaaatgacaa agcttgattt ttttaaaaac aactgtagaa atgtttgtgc
     6241 aaattcaata attcaactta aaatataatt ttacagctct gttcaataaa gcctctttcc
     6301 gggtgaggag cgataagcgg tatgtgtgtt tgttgggcaa tatttactta tcattacaat
     6361 gagattagtt atgcaattta ttggttatat tatcttggga taaatcatca gtgcttcata
     6421 cacacttgat gtctgcagga acaaatttta aagaacatac aacacattct cttttatttg
     6481 ttaaacacat aagtaaaaaa atgttgtact ctgtaggtaa aatgcaaacc gacatgcaag
     6541 ttaaaattta ttgttaaatt tcatagtcgg gtcaaaaggg gacatatcag aataccaaca
     6601 tagtgataga atcaatatga cccaagtccc ttaactcaca caaaggaagt aaatgattat
     6661 cgtcatttat ttgaattaat tagtcatatt gaaattaaaa atttttttca aagtcatcca
     6721 tctattctat caaaccaaag atttgcagca ggtgataact gggcacttag catgggaaag
     6781 atctgcattc acctgcattt ccagcataca gccacccacc ccagttctgt gctccccaag
     6841 atgagtaaaa cacacgttta ttttattagg tcttttttca ttctctctgc caggacactg
     6901 atatgtgtct cctgtttaaa gagagcctct atttgtcatc ttctttcaat tgcctttaag
     6961 ctgatttgcg tgaggctttt gtaaatgaac ttaaaatgga aaaacccctt aaatgttgaa
     7021 ttttggaagg tacacatgtc atgtcaagaa aactcacagg gttttttttc cttctcagtt
     7081 tttccaagca aagaagctgt tgtgagtgaa agtttcaatt gtatcattaa gcatttatca
     7141 aacgttaaag tagttcctaa atttccacat agtaagagaa cacactccag aagttaaaga
     7201 gaactggctg gtactccaaa attaattttt attaaacatc taagaagttg cagatggttg
     7261 tgaagaacaa ggggcaatca gaggggaaag cagacagact agcgaggtgg tgagaaactg
     7321 aatttctcgc atgagattta caatgattta catcagttag tcaaaccaac agaatcacgg
     7381 tttttacatc agatcaaatg ctcagttgct ttaatgaaag taaacatcta aaaatgtgga
     7441 aaatttttaa atgattagga taaatgaact ggaaactttg tgaaatcatc ttgtctttaa
     7501 tcgaagtaat gattgtggaa cccttaaaac actttcccat tcctaacatt gttatattta
     7561 tcaaaactgg acccaatgac acgttcccct taccattttg ctaatgtgtc agctataatt
     7621 tgagaagcct cgtgggttcc tgattttttt ttttaaactg gggaagcatt tagcaatcag
     7681 ggttttattt tctgggagaa atgatatgct gtgtcatctc cagttattga cagtaactga
     7741 tctgcattag ttaggagtgg taccgctttc atcagttcaa tgatgctact ttacaggtta
     7801 acatttctag agttgggcct gttttcattg caattagcag cactgtcatg tagtgatatg
     7861 gagcatgcac acagagctga gaggacttga gtactgtcag atgtgacaaa caagactaaa
     7921 tatgatcttt ccttgaaagt taaaagtgag aaaaaaatat tgcccaatca cacttatcag
     7981 gtgtatgaat aatcatggac tatgcagttt tgaaaaaaat aacctactta cttttccttt
     8041 cttttaaaat acgtcttttt tttaggatga tttacataca tacctaccca tgattttcca
     8101 ttttctacct tttttcctgt tatgtgtatt tagagttcca gcatggacta tatgtatcac
     8161 tggtctgctg tggtccctgg tgccatgggg cttatggtat tcaacagatt atgagtttgg
     8221 cccatgattt atattcctgt aagagaactg atggatgctt ggttacaaag tcagacagcg
     8281 gaccttgaca ctaatggcac ttcacatttc ccacagaatt ggctcagcat agcaatgaat
     8341 tatatctgaa ttagaatcta actagtctgt tttaccatgt aatatacaat ggaaacaatt
     8401 ccattcttta cttggacata tgataaaata taaattacag ctgtactaac ttttcaccag
     8461 acatgcaatt ttattctaaa tactttataa taataaactt atctgcaaac aaaatgtgta
     8521 atttgaataa taattaattc atttatttct atactataaa catgactgtg gtattactca
     8581 gaatcatttt aattttgtgt ttctcttggt gattttaaag ttggtgacag actggtctga
     8641 ttttggataa aacaaaactt ttcaaatgat cctcctaaat ttgtgttcct tattacaaaa
     8701 cctattttgt ttactgtgcc tttcaaatat cagggccact gaatagacat ttaaactgga
     8761 aaaaaaaatt aaccttcccc aaatatattc tagttgctga ctacttccac ggtttcagct
     8821 gtgtctcaac aaagcagaag gtacactctt cttgcaactg ggcagttgat ttcaatgagg
     8881 agttattgca ttttctgtga aaacaataag atcatagggt tcaatcccaa actatttctt
     8941 ttcctacaac attccctttg tgactagccc atttaattca ttttcttagc gttactttta
     9001 gaaaggaatg tctgtccttt tctactgtct tagaagaaaa actactacaa aataaatttg
     9061 atttttttca aatctcatag gaaaagtttg ttgtatattc atagtatatc tacatataat
     9121 tgtatgtgtt attacatgag taaagaaaaa ttaaatcttg acactataat gtgaagtatg
     9181 tcagccaggg aagtccctag tgggttaatg tctgcttaga aatagaagtg agagattgag
     9241 ataaagcttc cacatatttc tatatgaatt acttcatgag catatggtca tttgaaaatt
     9301 aatacgtcta tgagtagtga tttcactact ctgaacccat attgagaatc tcaaactgga
     9361 atccaaaatt tggaataggg ctgaggttaa actctgggta gaaactgcaa tagttttctc
     9421 aaggatatat ttgcaactga ttgaatttaa tttcaattgt actaattttc catgagttga
     9481 tttgcaatgt attcttattt ttcaataaaa cgttgggaaa ttccatgagt agggagaagc
     9541 ttggtagttt tgcttgtctt cgtatggctc ttcattgttg ccctgggatt ccgggctttc
     9601 ctttttattt cctgagtctc aacagtatat ttaaatgtat aagtttaatc aaatatgcat
     9661 tgtgttaaat ttatatgaaa cattggagac cataaaattt agatttatct taatttacat
     9721 tttgatattt tccttcttcc aaatagtttc agatagttgt atgttatttt ccttggaatt
     9781 tttttcaata aatgaaatta tgttaaatac tct
//
