LOCUS       NM_001010833            7372 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus mediator of DNA damage checkpoint 1 (Mdc1), transcript
            variant 1, mRNA.
ACCESSION   NM_001010833 XM_484632
VERSION     NM_001010833.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7372)
  AUTHORS   Leem,J., Kim,J.S. and Oh,J.S.
  TITLE     Oocytes can repair DNA damage during meiosis via a
            microtubule-dependent recruitment of CIP2A-MDC1-TOPBP1 complex from
            spindle pole to chromosomes
  JOURNAL   Nucleic Acids Res 51 (10), 4899-4913 (2023)
   PUBMED   36999590
  REMARK    GeneRIF: Oocytes can repair DNA damage during meiosis via a
            microtubule-dependent recruitment of CIP2A-MDC1-TOPBP1 complex from
            spindle pole to chromosomes.
REFERENCE   3  (bases 1 to 7372)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7372)
  AUTHORS   Castaneda-Zegarra,S., Xing,M., Gago-Fuentes,R., Saeterstad,S. and
            Oksenych,V.
  TITLE     Synthetic lethality between DNA repair factors Xlf and Paxx is
            rescued by inactivation of Trp53
  JOURNAL   DNA Repair (Amst) 73, 164-169 (2019)
   PUBMED   30579708
REFERENCE   5  (bases 1 to 7372)
  AUTHORS   Testa,E., Nardozi,D., Antinozzi,C., Faieta,M., Di Cecca,S.,
            Caggiano,C., Fukuda,T., Bonanno,E., Zhenkun,L., Maldonado,A.,
            Roig,I., Di Giacomo,M. and Barchi,M.
  TITLE     H2AFX and MDC1 promote maintenance of genomic integrity in male
            germ cells
  JOURNAL   J Cell Sci 131 (6) (2018)
   PUBMED   29437857
  REMARK    GeneRIF: Mdc1 was dispensable for the synapsis of the autosomes and
            played only a minor role in X-Y synapsis, compared with the action
            of H2afx.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7372)
  AUTHORS   Dimitrova,N. and de Lange,T.
  TITLE     MDC1 accelerates nonhomologous end-joining of dysfunctional
            telomeres
  JOURNAL   Genes Dev 20 (23), 3238-3243 (2006)
   PUBMED   17158742
  REMARK    GeneRIF: MDC1 knockdown affected the formation of telomere
            dysfunction-induced foci.
REFERENCE   7  (bases 1 to 7372)
  AUTHORS   Lou,Z., Minter-Dykhouse,K., Franco,S., Gostissa,M., Rivera,M.A.,
            Celeste,A., Manis,J.P., van Deursen,J., Nussenzweig,A., Paull,T.T.,
            Alt,F.W. and Chen,J.
  TITLE     MDC1 maintains genomic stability by participating in the
            amplification of ATM-dependent DNA damage signals
  JOURNAL   Mol Cell 21 (2), 187-200 (2006)
   PUBMED   16427009
  REMARK    GeneRIF: MDC1, as a signal amplifier of the ATM pathway, is vital
            in controlling proper DNA damage response and maintaining genomic
            stability.
REFERENCE   8  (bases 1 to 7372)
  AUTHORS   Lee,A.C., Fernandez-Capetillo,O., Pisupati,V., Jackson,S.P. and
            Nussenzweig,A.
  TITLE     Specific association of mouse MDC1/NFBD1 with NBS1 at sites of
            DNA-damage
  JOURNAL   Cell Cycle 4 (1), 177-182 (2005)
   PUBMED   15611643
  REMARK    GeneRIF: identification and description of a functional homologue
            of human MDC1/ NFBD1; in response to ionizing radiation it forms
            foci that co-localize with the MRE11-RAD50-NBS1 (MRN) complex and
            factors such as gammaH2AX and 53BP1
REFERENCE   9  (bases 1 to 7372)
  AUTHORS   Polci,R., Peng,A., Chen,P.L., Riley,D.J. and Chen,Y.
  TITLE     NIMA-related protein kinase 1 is involved early in the ionizing
            radiation-induced DNA damage response
  JOURNAL   Cancer Res 64 (24), 8800-8803 (2004)
   PUBMED   15604234
REFERENCE   10 (bases 1 to 7372)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CR974451.9, BY022698.1,
            AK033009.1, BC094363.1 and BC085140.1.
            
            On Mar 15, 2007 this sequence version replaced NM_001010833.1.
            
            Summary: The protein encoded by this gene contains an N-terminal
            forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central
            domain with 7 divergent copies of an approximately 41-amino acid
            sequence. The encoded protein is required to activate the intra-S
            phase and G2/M phase cell cycle checkpoints in response to DNA
            damage. This nuclear protein interacts with phosphorylated histone
            H2AX near sites of DNA double-strand breaks through its BRCT
            motifs, and facilitates recruitment of the ATM kinase and meiotic
            recombination 11 protein complex to DNA damage foci. Mice with
            mutations in this gene exhibit growth retardation, male
            infertility, immune defects, chromosome instability, DNA repair
            defects, and radiation sensitivity. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849374,
                              SAMN00849375 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-149               CR974451.9         65893-66041
            150-300             BY022698.1         1-151
            301-350             AK033009.1         138-187
            351-351             CR974451.9         70263-70263
            352-1289            AK033009.1         189-1126
            1290-1297           CR974451.9         72229-72236
            1298-5415           BC094363.1         1-4118
            5416-7372           BC085140.1         3285-5241
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 18.7 cM"
     gene            1..7372
                     /gene="Mdc1"
                     /gene_synonym="6820401C03; mKIAA0170; Nfbd1"
                     /note="mediator of DNA damage checkpoint 1"
                     /db_xref="GeneID:240087"
                     /db_xref="MGI:MGI:3525201"
     exon            1..182
                     /gene="Mdc1"
                     /gene_synonym="6820401C03; mKIAA0170; Nfbd1"
                     /inference="alignment:Splign:2.1.0"
     exon            183..321
                     /gene="Mdc1"
                     /gene_synonym="6820401C03; mKIAA0170; Nfbd1"
                     /inference="alignment:Splign:2.1.0"
     CDS             186..5312
                     /gene="Mdc1"
                     /gene_synonym="6820401C03; mKIAA0170; Nfbd1"
                     /note="isoform a is encoded by transcript variant 1;
                     mediation of DNA damage checkpoint 1"
                     /codon_start=1
                     /product="mediator of DNA damage checkpoint protein 1
                     isoform a"
                     /protein_id="NP_001010833.2"
                     /db_xref="CCDS:CCDS37604.1"
                     /db_xref="GeneID:240087"
                     /db_xref="MGI:MGI:3525201"
                     /translation="MESTQVIDWDAEEEEETELSSGSLGYSVEPIGQLRLFSGTHGPE
                     RDFPLYLGKNVVGRSPDCSVALPFPSISKQHAVIEISAWNKAPILQDCGSLNGTQIVK
                     PPRVLPPGVSHRLRDQELILFADFPCQYHRLDVPPPLVPRSLLTIEKTPRIRIESQNS
                     RVLLAADSEEEGDFPSGRCVANGQRNTASPSATVVPESDEEVSSPAPSVPGPSSPFGL
                     GSDTDEEQGQQPGVEESSLADSSGAAGEAEQPEANGTTAGIQAQPTEHKLKDTKVKKE
                     AGRAGVSDGSVLERSPTLGEDSDTEVDEDHKPGFADSETDVEEERIPVTPPVAPVKKN
                     QVLLAVGIGDPEAPGVAHLQDCLAGSGTDVEDKTALDVPLERNHTPMVINSDTDEEEE
                     EEEEEVSAALTLAHLKERGIGLWSRDPGAEEVKSQPQVLVEQSQSASGRDSDTDVEEE
                     SSGRKREIIPDSPMDVDEALTVTQPESQPPRRPNDADEYMDMSSPGSHLVVNQASFAV
                     VGKTRAQVEEEVPGPSVILGEKHQVPLEGAQPPEEAWETAVQEGSSSPEAAASVRPSQ
                     QPVAEDAGTECATAVSEQESTLEVRSQSGSPAAPVEQVVIHTDTSGDPTLPQREGAQT
                     PTGREREAHVGRTKSAKECCDAEPEDLCLPATQCFVEGESQHPEAVQSLENEPTQLFP
                     CTLPQEPGPSHLSLQTPGADTLDVPWEVLATQPFCLREQSETSELHEAHGSQPSLPRE
                     PPGHQHLVHTSPVHTELLRIEGREIQTVEKAMGIPKEMADRMTPEREPLEREIRGRTE
                     NSERDVIGEELIQGTKDREPKKVLARDSQRKEADKDLEGNRESLEVEIEMSKDSQKRE
                     RKVEKPEPKREWEPADLEVTPDRGVTEEGSHDQKGQIASLTLKPGVGVKDLEGLASAP
                     IITGSQADGGKGDPLSPGRQQRGRLSCQTTPAGKASRGDPEPPDHCLFSSVPEASTQS
                     LLTSQSQKQSTPQPLFSTSSSEIPLPESLHTKPNVRPRRSSRMTPSPHSSAALKPNTT
                     CPTNQPAASRPTSRPTRGRANRSSTRTPELIVPVDPELQPSTSTEQPVIPKLTSQVTE
                     GRVQMPEPLLTGPEIQSPTSTEQSVTPDRKPRATRGRPSKSPNKTPEPLISTGPELQP
                     PTSIEQPVIPKPTSRVTRGRPRKSSVRTPESVVSTGPELQPLTSIEQPVIPEPRATRG
                     RPSKSSIKTPESVVPTGPELQPLTSAKQPVTPNLTSRASRGRSSKSIRTPEPVVQTGP
                     EFHPSTSTEQPDTREPSSQARTRRSAVKTPEASVPTTPELQPFTSKKQPAPKPTALVT
                     QGRTYKPSTEDCESVGPVAPDFEPSTSTDHLVTPKVTDQSLTLQSSPLSASPVSSTPD
                     LKPPVPIAQPVTPEPIPQANHQRKRRAAGKQGSRTVPLGHKSYSALSEPEPQSSASQS
                     SGASEADSPRQKRPRRQASQKTVVIKEEPVETEVKEEPQETAIPTPEKRKRDHAEEVT
                     QGKPTRSRRTKPNQETAPKVLFTGVMDSRGERAVLALGGSLASSVNEASHLVTDRIRR
                     TVKFLCALGKGIPILSLNWLYQSRKAGCFLPPDDYLVTDPEQEKNFSFSLRDSLCRAR
                     ERRLLEDYEIHVTPGVQPPPPQMGEIISCCGGTFLPSMPHSYKLHRVIITCTEDLPRC
                     AIPSRLGLPLLSPEFLLTGVLKQEATPEAFVLSNLEMSST"
     exon            322..702
                     /gene="Mdc1"
                     /gene_synonym="6820401C03; mKIAA0170; Nfbd1"
                     /inference="alignment:Splign:2.1.0"
     exon            703..778
                     /gene="Mdc1"
                     /gene_synonym="6820401C03; mKIAA0170; Nfbd1"
                     /inference="alignment:Splign:2.1.0"
     exon            779..2121
                     /gene="Mdc1"
                     /gene_synonym="6820401C03; mKIAA0170; Nfbd1"
                     /inference="alignment:Splign:2.1.0"
     exon            2122..2190
                     /gene="Mdc1"
                     /gene_synonym="6820401C03; mKIAA0170; Nfbd1"
                     /inference="alignment:Splign:2.1.0"
     exon            2191..2283
                     /gene="Mdc1"
                     /gene_synonym="6820401C03; mKIAA0170; Nfbd1"
                     /inference="alignment:Splign:2.1.0"
     exon            2284..2994
                     /gene="Mdc1"
                     /gene_synonym="6820401C03; mKIAA0170; Nfbd1"
                     /inference="alignment:Splign:2.1.0"
     exon            2995..3038
                     /gene="Mdc1"
                     /gene_synonym="6820401C03; mKIAA0170; Nfbd1"
                     /inference="alignment:Splign:2.1.0"
     exon            3039..4512
                     /gene="Mdc1"
                     /gene_synonym="6820401C03; mKIAA0170; Nfbd1"
                     /inference="alignment:Splign:2.1.0"
     exon            4513..4604
                     /gene="Mdc1"
                     /gene_synonym="6820401C03; mKIAA0170; Nfbd1"
                     /inference="alignment:Splign:2.1.0"
     exon            4605..4724
                     /gene="Mdc1"
                     /gene_synonym="6820401C03; mKIAA0170; Nfbd1"
                     /inference="alignment:Splign:2.1.0"
     exon            4725..4907
                     /gene="Mdc1"
                     /gene_synonym="6820401C03; mKIAA0170; Nfbd1"
                     /inference="alignment:Splign:2.1.0"
     exon            4908..5033
                     /gene="Mdc1"
                     /gene_synonym="6820401C03; mKIAA0170; Nfbd1"
                     /inference="alignment:Splign:2.1.0"
     exon            5034..5144
                     /gene="Mdc1"
                     /gene_synonym="6820401C03; mKIAA0170; Nfbd1"
                     /inference="alignment:Splign:2.1.0"
     exon            5145..7352
                     /gene="Mdc1"
                     /gene_synonym="6820401C03; mKIAA0170; Nfbd1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7331..7336
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mdc1"
                     /gene_synonym="6820401C03; mKIAA0170; Nfbd1"
                     /note="hexamer: AATAAA"
     polyA_site      7352
                     /gene="Mdc1"
                     /gene_synonym="6820401C03; mKIAA0170; Nfbd1"
                     /note="major polyA site"
ORIGIN      
        1 gcacatttga gctgtgagga ggtgaggcag gggcggggct tccgacttga atcaatcggc
       61 gcgcttccag cagtaaaaga cagttccagg agccaatcag cccaccgact ctcttccggg
      121 attcgcactc ccgccacctg gtagcggccg ttactggtgg cgcgcgcgcg cggattcagt
      181 cgatcatgga aagcacccag gtgattgact gggatgctga agaagaggaa gagacagaac
      241 tatccagtgg ctccttgggg tatagtgtgg agcctatagg gcagctacgt ctcttcagtg
      301 gaactcatgg accagaaaga gatttccccc tctaccttgg caagaatgta gttggtcgaa
      361 gccctgactg ctctgtggcc ctgccttttc catccatctc caaacagcat gcagtaattg
      421 aaatctcagc ttggaacaaa gcccctatcc tccaggattg tgggagcctc aatggcactc
      481 aaatcgtaaa gcctcctagg gtcctacctc ctggagtgag ccatcgtctg agggaccagg
      541 aattaattct gtttgcagat tttccctgcc agtaccatcg cctggatgtc ccaccaccct
      601 tggtccctcg gagtcttcta actatagaga aaacccccag aatacggata gaatcccaaa
      661 attccagagt tttgttggct gccgattcag aggaagaagg ggattttcct tctggaaggt
      721 gtgtggcgaa tggacaaagg aatacagcat ccccttcagc aacagtagtt ccagaaagtg
      781 atgaagaggt ctcttctccg gccccaagtg tccccgggcc atcttcaccc tttggtttgg
      841 gcagtgacac tgatgaagaa caaggtcagc aaccaggagt agaggagtcc tctttagctg
      901 acagcagtgg tgctgcaggg gaagctgagc agcctgaagc taacggaacg acagctggca
      961 tacaggctca gcctactgag cacaaattga aggacacaaa agtcaagaag gaggcaggca
     1021 gggcaggggt ctcagatggg tcagttctgg agaggagccc cactcttgga gaggacagtg
     1081 acacagaagt ggacgaagat cacaaacctg ggtttgcgga cagtgagaca gatgtggaag
     1141 aagagaggat ccctgtgacc ccccctgtag cccctgtgaa gaaaaaccaa gtgctacttg
     1201 cagttggtat aggagatcct gaagcacctg gtgtggcaca tctgcaggac tgcctagctg
     1261 gtagtggcac agatgtggag gacaagactg cactggatgt ccctctagag agaaaccaca
     1321 cacccatggt gatcaacagt gatacagatg aggaggagga ggaggaggag gaggaggtct
     1381 cagcagcact cactttggcc catctgaaag agagaggaat tggtttgtgg agtagagacc
     1441 caggcgcaga agaggtcaag tcccaaccac aggtccttgt agaacaaagc cagagtgcct
     1501 ctgggagaga cagtgacacg gatgtggagg aggaatcctc agggcgaaag agagagatta
     1561 tcccagacag tcccatggat gtagatgagg ctcttactgt cacacagcca gagagccagc
     1621 ctccccgtag gcccaatgat gcagatgagt acatggatat gagctcccct ggcagccatc
     1681 tagtggtaaa tcaggcctcc tttgctgtgg taggcaagac cagagcacaa gtggaggagg
     1741 aagtcccagg gccatctgtt atcctggggg agaagcacca ggtgcctcta gagggagccc
     1801 agcctcctga ggaagcctgg gaaacagctg ttcaggaagg ctcatcctca ccagaggcag
     1861 cagcctctgt aaggcctagc cagcagccag tagcagaaga tgctgggaca gagtgtgcta
     1921 cagctgtttc tgagcaggaa agtactcttg aggtgaggtc ccaaagcggg tcacctgcag
     1981 caccagtgga gcaagtggtg atacatacag atacttcagg ggatcccacc ctgccacaga
     2041 gagagggagc ccaaaccccc acaggaaggg agagagaagc acatgtgggc aggaccaaga
     2101 gtgccaaaga gtgctgtgat gcagaacctg aagatctgtg ccttccagct acccagtgct
     2161 ttgtggaagg ggagagccag cacccagaag ctgtccagag tttggaaaat gagcctaccc
     2221 aactcttccc atgtactctt ccacaagagc caggaccttc ccatctcagc cttcagactc
     2281 caggtgcaga taccttggat gtgccttggg aagtcttggc tacacagccg ttctgtctaa
     2341 gagaacagtc agagacttct gagcttcatg aagctcatgg atctcaacca tctctgccta
     2401 gggaaccacc aggacaccaa catctggttc ataccagccc agttcacaca gagctgttga
     2461 gaattgaagg cagagagata cagactgtgg agaaagccat gggtatacca aaggaaatgg
     2521 cagacaggat gacccctgag agagagccat tggagaggga aatcagggga agaacagaaa
     2581 acagtgagag agatgtgata ggagaagaac taattcaggg gacaaaggac agagagccaa
     2641 agaaggtgtt agctagagac agtcagagaa aagaggctga caaggatctt gaagggaata
     2701 gggagagttt ggaggtagaa atagagatgt ccaaagactc acagaagaga gagagaaaag
     2761 tagagaaacc agaaccaaag agagaatggg agccagctga tttagaagtg actccagata
     2821 gaggggtgac agaggaaggg agccatgatc agaaagggca aattgctagt ctaacactaa
     2881 agcctggagt tggggtgaag gaccttgagg gacttgcatc agccccaata atcacaggga
     2941 gccaggcaga tggaggaaag ggagaccctt tgagccctgg gaggcagcag agaggccgct
     3001 tgagttgcca gacgacacct gctggaaagg cttccagggg tgatccagag cccccagacc
     3061 actgcctgtt ttcttcagtg cctgaagctt caactcagag tctcctcacc tctcagagcc
     3121 aaaagcagtc tacacctcag cctctgtttt cgacctcttc ttctgagata ccccttcctg
     3181 agagtcttca cacaaagcct aatgtcaggc cacggcggtc ctccaggatg accccctccc
     3241 cgcattcctc tgctgccctt aagccaaata ctacctgccc cacaaaccag cctgctgcct
     3301 ctagaccaac atcccgaccc actcggggca gggcaaatag gtcctctacc aggaccccag
     3361 aactgattgt ccctgtagac cctgaactcc agccttccac ctccacagaa cagcctgtca
     3421 tcccaaaact tacgtcccag gtcactgagg gcagggtaca gatgcctgaa ccacttctca
     3481 caggtcccga gatccagtct cccacctcca cagaacagtc tgtcacccca gaccgcaaac
     3541 ctcgggccac tcggggcagg ccaagtaaat ctcccaacaa gaccccagaa ccacttattt
     3601 ctactggccc tgaactccaa cctcccacct ccatagaaca acctgtcata cctaaaccca
     3661 cttctcgggt cactcggggc agaccacgta agtcttctgt caggacccca gaatcagttg
     3721 tctccactgg ccctgaactt cagcctctca cctctataga acagcctgtt atccctgaac
     3781 ctagggccac tcgaggtaga ccaagtaagt cttctatcaa gaccccagaa tcagttgtcc
     3841 ccactggtcc tgagctgcag cctcttacct ctgcaaaaca gcctgtcacc cctaacctca
     3901 catctcgggc ctctcggggt agatcaagta agtctatcag gaccccggaa ccagttgtcc
     3961 aaactggccc tgaattccat ccatccacct ccacagaaca gcctgatacc cgtgagccct
     4021 catctcaggc taggacacgt aggtctgctg tcaagactcc tgaagcgagt gtccccacaa
     4081 cccctgaact ccagcctttc acttctaaaa aacagcctgc ccctaagcct acagctctgg
     4141 tcactcaggg aaggacatat aaaccctcca ctgaagattg tgaatcagtt gggccagtgg
     4201 cccctgattt tgagccttcc acctctacag accatcttgt cacccctaag gttacagatc
     4261 agagcttaac actacagtct tcacccctaa gtgcttcccc agtttccagt acccctgatc
     4321 tcaagcctcc tgtccccata gcccagcctg ttaccccaga gcccattcca caagccaatc
     4381 accaaaggaa acgaagggct gctgggaagc agggttcccg cacagtccct ctgggtcata
     4441 agtcttactc tgccctctct gaacctgagc cccaatcttc agccagccaa agctcaggag
     4501 catcagaagc agactcaccc cgtcaaaaac gtcccagaag acaagcctcc cagaaaactg
     4561 tagtcatcaa ggaagaacct gtagaaacag aagtgaagga agagcctcaa gagactgcaa
     4621 ttccaacacc agaaaagaga aagagggacc atgcagaaga agtgacccag ggaaaaccaa
     4681 ctcgaagccg gcgaactaaa cctaaccaag aaacagcccc caaggtactg ttcacaggag
     4741 tcatggattc tcgtggcgag cgtgcagtgc tggctctggg aggcagtctg gccagctcag
     4801 taaatgaggc ctcccactta gttactgatc gcatccgcag gacagtcaag ttcttgtgtg
     4861 ccctggggaa gggaattccc atcctgtccc tgaactggct gtaccagtcc cgaaaggctg
     4921 gttgcttctt gccgcctgat gactacttag tgactgatcc tgagcaagag aagaacttta
     4981 gcttcagcct tcgggattcc ctgtgccggg ctcgggaacg aagactgctg gaggactatg
     5041 agattcatgt gacccctggc gtgcagccac ccccccctca gatgggcgag atcatcagtt
     5101 gctgtggagg cactttcctg cccagcatgc ctcactccta taagcttcac cgagtcatca
     5161 taacttgcac tgaagaccta ccacgctgtg ctattccatc ccgcctgggg ctacccctcc
     5221 tctctcctga gttcctcctg actggagtgc tgaagcagga agccacacca gaggcctttg
     5281 ttctctccaa tttggaaatg tcatctacct aaaaaaatat gtacctttac cttctcagac
     5341 catcgaacag tcctagaaaa cacttggaaa aaggaacaca cacatacaca attaagacgg
     5401 ggcaaggtgg ttcacacctt taatctcagc acttgggagg cagaagtaat catatttttc
     5461 tgagttctgg gctagccaga ggtacataat gagaccccat ctcaaaaacc aaatcaaaca
     5521 acaaacctca gggtttcaca aaggcctaag gtatattgta tcatcgtaac tgtttgggga
     5581 atgtgaataa ggcctaaaat gttttgggga aaaaagggaa cttttttcct aagatagcca
     5641 gttttttagt tggctcacct gagctgggta tggctctcta ccgagcacct gccatctgcc
     5701 cagctgtgct ccgaggaagc tgctccaggc tcagtgccct gactctacct cctgtccaga
     5761 agccagcatt ttctctttac ttcccggggt tctactcaac atgaagcagc tcccaggagt
     5821 gcttggttga gaagatgagg gtagctcatg ggtgtggcta ggaaaggctt tagttttgct
     5881 gtacttgctc tgctttgcag tgttggcagt tagctgtggt catctccagt agctcagtgc
     5941 tcactctgcc ttcttggtgg caggtttctg tgtggagaca caatggattt gaacttgcac
     6001 ttcatttatt attattatta atttagagta aattgtattt gttttttaaa gttggctact
     6061 ttccaggatg tggagaaagt agaactcatg aatactgctg gtgtttggct tgtcctctgc
     6121 agtaaacagt agcaaagtta tttcaaagta agcagagcta gccgtgggca ttgcctgtag
     6181 ccccagtgct taggaggctg aagtaggaag ctcaccagtg ccagttcagc ctgggctaca
     6241 gcgtgagagt cgggcgtggt ggcgcactcg ggaggcagac gcaggcagag ctctgatttc
     6301 aaggccagcc tgggctacag agtgagttct agaacagtca gggctgttaa cagagtaaat
     6361 cctgtctcga aagagaagga aaaaattttt tttggtttgt tttttgccat tttctctgga
     6421 catgtgtaag gtgagtcagt gctcgttgtg cctgcctggt cactctatga cccagcaaga
     6481 ctgttctgta agcaaaaaaa aactctgttt tgtttggttt tgtttcttcg agacagggtt
     6541 tctctgtata gccctggctg tcctggaact cactttgtag accaggctgg ccttgaactc
     6601 agaaatctgc ctgcctctgc ctcccgagtg ctgggattaa aggcgtgtgc caccacgccc
     6661 ggctgcaaag aaaactttta aacatgcatt tggtatgtaa tcccagcagt ccctgtaaag
     6721 gctagtagga tcagaagttc aagctcatcg tggatttttg ggtttttgtt ttgtttttcc
     6781 gcccccaagg tcagctcagt ctaaactgca aaagattcaa aaatgaaaag cacttgatgt
     6841 tttatctttt tggttgggct ggggcttgaa cctggagctt ccttccttta tgcatgctag
     6901 gcaaagcctc taccactaaa ccttattccc agcccctcac ctctttccaa atatagaaaa
     6961 gctgaagatt tttgttcagc aaggatgagc atatatactg tctgcacaga aagcaatgtt
     7021 cagacatgaa gtgaaaacac agtatttgaa ggtggtcaaa gacaccctaa cacaagggcg
     7081 agacaaaagg gacaggtttg attccacctg cataataata tataaagtgt ggtgtgaatt
     7141 cacaaaggta agagcaaatt gatgcttgcc agaccatgag tacacagaag ctgttcaagg
     7201 ggtctagtgt tcccctgtgg ggagttgaaa gacttggaat cagaaagagg aagtgattgt
     7261 acataatgca gaatattaag tactaatgta taaaagggtt gggttttttt tgctttatga
     7321 atttcacttc aataaaaaag ttgcttactt taaaaaaaaa aaaaaaaaaa aa
//
