LOCUS       NM_025779               1191 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Mus musculus mitochondrial calcium uniporter dominant negative beta
            subunit (Mcub), transcript variant 1, mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_025779
VERSION     NM_025779.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Bround,M.J., Abay,E., Huo,J., Havens,J.R., York,A.J., Bers,D.M. and
            Molkentin,J.D.
  TITLE     MCU-independent Ca2+ uptake mediates mitochondrial Ca2+ overload
            and necrotic cell death in a mouse model of Duchenne muscular
            dystrophy
  JOURNAL   Sci Rep 14 (1), 6751 (2024)
   PUBMED   38514795
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1191)
  AUTHORS   Huo,J., Prasad,V., Grimes,K.M., Vanhoutte,D., Blair,N.S., Lin,S.C.,
            Bround,M.J., Bers,D.M. and Molkentin,J.D.
  TITLE     MCUb is an inducible regulator of calcium-dependent mitochondrial
            metabolism and substrate utilization in muscle
  JOURNAL   Cell Rep 42 (11), 113465 (2023)
   PUBMED   37976157
  REMARK    GeneRIF: MCUb is an inducible regulator of calcium-dependent
            mitochondrial metabolism and substrate utilization in muscle.
REFERENCE   4  (bases 1 to 1191)
  AUTHORS   Ghatge,M., Nayak,M.K., Flora,G.D., Kumskova,M., Jain,A.,
            Patel,R.B., Lin,Z., Usachev,Y.M. and Chauhan,A.K.
  TITLE     Mitochondrial calcium uniporter b deletion inhibits platelet
            function and reduces susceptibility to arterial thrombosis
  JOURNAL   J Thromb Haemost 21 (8), 2163-2174 (2023)
   PUBMED   37061131
  REMARK    GeneRIF: Mitochondrial calcium uniporter b deletion inhibits
            platelet function and reduces susceptibility to arterial
            thrombosis.
REFERENCE   5  (bases 1 to 1191)
  AUTHORS   Singh,R., Bartok,A., Paillard,M., Tyburski,A., Elliott,M. and
            Hajnoczky,G.
  TITLE     Uncontrolled mitochondrial calcium uptake underlies the
            pathogenesis of neurodegeneration in MICU1-deficient mice and
            patients
  JOURNAL   Sci Adv 8 (11), eabj4716 (2022)
   PUBMED   35302860
REFERENCE   6  (bases 1 to 1191)
  AUTHORS   Lambert,J.P., Luongo,T.S., Tomar,D., Jadiya,P., Gao,E., Zhang,X.,
            Lucchese,A.M., Kolmetzky,D.W., Shah,N.S. and Elrod,J.W.
  TITLE     MCUB Regulates the Molecular Composition of the Mitochondrial
            Calcium Uniporter Channel to Limit Mitochondrial Calcium Overload
            During Stress
  JOURNAL   Circulation 140 (21), 1720-1733 (2019)
   PUBMED   31533452
  REMARK    GeneRIF: MCUB-dependent changes in mitochondrial calcium uniporter
            stoichiometry are a prominent regulatory mechanism to modulate
            mitochondrial Ca(2+) uptake and cardiac myocyte cellular
            physiology.
REFERENCE   7  (bases 1 to 1191)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   8  (bases 1 to 1191)
  AUTHORS   Markus,N.M., Hasel,P., Qiu,J., Bell,K.F., Heron,S., Kind,P.C.,
            Dando,O., Simpson,T.I. and Hardingham,G.E.
  TITLE     Expression of mRNA Encoding Mcu and Other Mitochondrial Calcium
            Regulatory Genes Depends on Cell Type, Neuronal Subtype, and Ca2+
            Signaling
  JOURNAL   PLoS One 11 (2), e0148164 (2016)
   PUBMED   26828201
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 1191)
  AUTHORS   Raffaello,A., De Stefani,D., Sabbadin,D., Teardo,E., Merli,G.,
            Picard,A., Checchetto,V., Moro,S., Szabo,I. and Rizzuto,R.
  TITLE     The mitochondrial calcium uniporter is a multimer that can include
            a dominant-negative pore-forming subunit
  JOURNAL   EMBO J 32 (17), 2362-2376 (2013)
   PUBMED   23900286
  REMARK    GeneRIF: Functional characterization of the MCUb protein; shows
            that it inhibits mitochondrial calcium uptake
REFERENCE   10 (bases 1 to 1191)
  AUTHORS   Quina,L.A., Wang,S., Ng,L. and Turner,E.E.
  TITLE     Brn3a and Nurr1 mediate a gene regulatory pathway for habenula
            development
  JOURNAL   J Neurosci 29 (45), 14309-14322 (2009)
   PUBMED   19906978
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC118620.10.
            
            On Dec 21, 2021 this sequence version replaced NM_025779.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK018492.1, AK150995.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG   :: upstream AUG and CDS
                                                  extension is not conserved
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on manual assertion,
                                                  conservation, expression,
                                                  longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-130               AC118620.10        103377-103506
            131-206             AC118620.10        138756-138831
            207-377             AC118620.10        139703-139873
            378-482             AC118620.10        146987-147091
            483-643             AC118620.10        154710-154870
            644-847             AC118620.10        156314-156517
            848-964             AC118620.10        157637-157753
            965-1191            AC118620.10        158270-158496
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 59.56 cM"
     gene            1..1191
                     /gene="Mcub"
                     /gene_synonym="9030408N13Rik; Ccdc109b"
                     /note="mitochondrial calcium uniporter dominant negative
                     beta subunit"
                     /db_xref="GeneID:66815"
                     /db_xref="MGI:MGI:1914065"
     exon            1..130
                     /gene="Mcub"
                     /gene_synonym="9030408N13Rik; Ccdc109b"
                     /inference="alignment:Splign:2.1.0"
     CDS             32..1042
                     /gene="Mcub"
                     /gene_synonym="9030408N13Rik; Ccdc109b"
                     /note="isoform 1 is encoded by transcript variant 1;
                     coiled-coil domain-containing protein 109B; mitochondrial
                     calcium uniporter regulatory subunit MCUb; coiled-coil
                     domain containing 109B; calcium uniporter regulatory
                     subunit MCUb, mitochondrial"
                     /codon_start=1
                     /product="calcium uniporter regulatory subunit MCUb,
                     mitochondrial isoform 1"
                     /protein_id="NP_080055.3"
                     /db_xref="CCDS:CCDS17839.1"
                     /db_xref="GeneID:66815"
                     /db_xref="MGI:MGI:1914065"
                     /translation="MLPSGLCLGRWQLLRTIRARGRGDPRELPSTPQVLCMKLYGNPK
                     YHQALHYGTVEPQDEITVTYKHGLPLVTLTLPSRKERCQFVVKPMLSTVGSFLQDLQN
                     EDKGIKTAAIITADGSEIPASTLMDTLLMTDFKLIINKLRYDIRCHKKEEPSGEHMTE
                     LENTKSLVHRLFTILHLEEIQKRRERHLMAKIDHLQEQLRPLEQVKAAIEARSEANTS
                     GLLWAGLALLSVQGGALAWLTWWVYSWDIMEPVTFFLSFANSIVFFAYFIITRQNYTY
                     SSLRSRQFLQFFHKKSQRRCFDVEQYNKLKEDLAEATESLESVRRSLRLRIQGEEASE
                     KN"
     exon            131..206
                     /gene="Mcub"
                     /gene_synonym="9030408N13Rik; Ccdc109b"
                     /inference="alignment:Splign:2.1.0"
     exon            207..377
                     /gene="Mcub"
                     /gene_synonym="9030408N13Rik; Ccdc109b"
                     /inference="alignment:Splign:2.1.0"
     exon            378..482
                     /gene="Mcub"
                     /gene_synonym="9030408N13Rik; Ccdc109b"
                     /inference="alignment:Splign:2.1.0"
     exon            483..643
                     /gene="Mcub"
                     /gene_synonym="9030408N13Rik; Ccdc109b"
                     /inference="alignment:Splign:2.1.0"
     exon            644..847
                     /gene="Mcub"
                     /gene_synonym="9030408N13Rik; Ccdc109b"
                     /inference="alignment:Splign:2.1.0"
     exon            848..964
                     /gene="Mcub"
                     /gene_synonym="9030408N13Rik; Ccdc109b"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1191
                     /gene="Mcub"
                     /gene_synonym="9030408N13Rik; Ccdc109b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1149..1154
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mcub"
                     /gene_synonym="9030408N13Rik; Ccdc109b"
                     /note="hexamer: AATAAA"
     polyA_site      1175
                     /gene="Mcub"
                     /gene_synonym="9030408N13Rik; Ccdc109b"
                     /note="major polyA site"
ORIGIN      
        1 gttaatgcca ggagctctgt ccggcaggag gatgctcccg agcgggctat gtctgggtcg
       61 gtggcagctg ctgcggacga tccgagcccg cggccgcggg gatcccaggg agctgccatc
      121 cacaccccag gttttatgta tgaaactcta tggaaatcca aaataccacc aagcactcca
      181 ttatgggaca gtggagccac aggatgaaat aacagttact tataaacacg gccttccctt
      241 ggtaaccctc actctgccat ccagaaaaga gcgctgccag tttgtagtca agccgatgtt
      301 gtcgacagta ggctcattcc tgcaggactt acagaatgag gataaaggca tcaaaactgc
      361 agccatcatc acggcagatg gcagtgaaat tccagcttca acattgatgg acactttgct
      421 aatgacagat tttaagctca tcattaacaa actacggtat gatatacggt gtcacaagaa
      481 agaagagcca agtggagagc atatgactga gttggaaaac accaagtcct tggttcacag
      541 actattcact atcctgcatt tagaggagat ccagaagaga cgagagcgcc atctgatggc
      601 gaagatcgac cacctgcagg aacagctgcg gcccttggag caggtcaaag ctgcaataga
      661 agcccggtcg gaagccaaca ccagtggtct cctgtgggcc ggcttagctc tgctctccgt
      721 gcagggcggg gcgctggcct ggcttacttg gtgggtgtac tcctgggata tcatggagcc
      781 agttaccttc ttcctgtcgt ttgcgaattc catcgtcttt tttgcatact tcatcataac
      841 ccggcagaac tacacatact catccctccg gagcaggcag tttcttcagt tcttccacaa
      901 gaaatcgcag cggcggtgtt ttgatgtgga acaatacaac aagctaaagg aagaccttgc
      961 tgaggctaca gaatccctgg aaagcgtgcg ccgctccctc cgcttgcgaa tccaaggaga
     1021 ggaagccagc gagaagaact agacacacac tttaaactca gcaggcgatg tgctggtctg
     1081 atagcttacc aactggatgt ttttaaggtc ttgttcattt ccgcttgacg ttttcataca
     1141 gtttggttaa taaatttgtg tgaaacagca aagtattcat tgacaaccgc a
//
