LOCUS       NM_001033259            2872 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus mitochondrial calcium uniporter (Mcu), mRNA; nuclear
            gene for mitochondrial product.
ACCESSION   NM_001033259 XM_125687 XM_895614 XM_895621 XM_904478 XM_918861
            XM_918871
VERSION     NM_001033259.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   LaMoia,T.E., Hubbard,B.T., Guerra,M.T., Nasiri,A., Sakuma,I.,
            Kahn,M., Zhang,D., Goodman,R.P., Nathanson,M.H., Sancak,Y.,
            Perelis,M., Mootha,V.K. and Shulman,G.I.
  TITLE     Cytosolic calcium regulates hepatic mitochondrial oxidation,
            intrahepatic lipolysis, and gluconeogenesis via CAMKII activation
  JOURNAL   Cell Metab 36 (10), 2329-2340 (2024)
   PUBMED   39153480
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Hasan,P., Berezhnaya,E., Rodriguez-Prados,M., Weaver,D.,
            Bekeova,C., Cartes-Saavedra,B., Birch,E., Beyer,A.M., Santos,J.H.,
            Seifert,E.L., Elrod,J.W. and Hajnoczky,G.
  TITLE     MICU1 and MICU2 control mitochondrial calcium signaling in the
            mammalian heart
  JOURNAL   Proc Natl Acad Sci U S A 121 (35), e2402491121 (2024)
   PUBMED   39163336
REFERENCE   3  (bases 1 to 2872)
  AUTHORS   Kadier,T., Zhang,Y.G., Jing,Y.X., Weng,Z.Y., Liao,S.S., Luo,J.,
            Ding,K., Cao,C., Chen,R. and Meng,Q.T.
  TITLE     MCU inhibition protects against intestinal ischemia-reperfusion by
            inhibiting Drp1-dependent mitochondrial fission
  JOURNAL   Free Radic Biol Med 221, 111-124 (2024)
   PUBMED   38763207
  REMARK    GeneRIF: MCU inhibition protects against intestinal
            ischemia-reperfusion by inhibiting Drp1-dependent mitochondrial
            fission.
REFERENCE   4  (bases 1 to 2872)
  AUTHORS   Lebas,M., Chinigo,G., Courmont,E., Bettaieb,L., Machmouchi,A.,
            Goveia,J., Beatovic,A., Van Kerckhove,J., Robil,C., Angulo,F.S.,
            Vedelago,M., Errerd,A., Treps,L., Gao,V., Delgado De la
            Herran,H.C., Mayeuf-Louchart,A., L'homme,L., Chamlali,M., Dejos,C.,
            Gouyer,V., Garikipati,V.N.S., Tomar,D., Yin,H., Fukui,H.,
            Vinckier,S., Stolte,A., Conradi,L.C., Infanti,F., Lemonnier,L.,
            Zeisberg,E., Luo,Y., Lin,L., Desseyn,J.L., Pickering,J.G.,
            Kishore,R., Madesh,M., Dombrowicz,D., Perocchi,F., Staels,B.,
            Pla,A.F., Gkika,D. and Cantelmo,A.R.
  TITLE     Integrated single-cell RNA-seq analysis reveals mitochondrial
            calcium signaling as a modulator of endothelial-to-mesenchymal
            transition
  JOURNAL   Sci Adv 10 (32), eadp6182 (2024)
   PUBMED   39121218
REFERENCE   5  (bases 1 to 2872)
  AUTHORS   Sasaki,H., Nakagawa,I., Furuta,T., Yokoyama,S., Morisaki,Y.,
            Saito,Y. and Nakase,H.
  TITLE     Mitochondrial Calcium Uniporter (MCU) is Involved in an Ischemic
            Postconditioning Effect Against Ischemic Reperfusion Brain Injury
            in Mice
  JOURNAL   Cell Mol Neurobiol 44 (1), 32 (2024)
   PUBMED   38568450
  REMARK    GeneRIF: Mitochondrial Calcium Uniporter (MCU) is Involved in an
            Ischemic Postconditioning Effect Against Ischemic Reperfusion Brain
            Injury in Mice.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2872)
  AUTHORS   Qiu,J., Tan,Y.W., Hagenston,A.M., Martel,M.A., Kneisel,N.,
            Skehel,P.A., Wyllie,D.J., Bading,H. and Hardingham,G.E.
  TITLE     Mitochondrial calcium uniporter Mcu controls excitotoxicity and is
            transcriptionally repressed by neuroprotective nuclear calcium
            signals
  JOURNAL   Nat Commun 4, 2034 (2013)
   PUBMED   23774321
  REMARK    GeneRIF: Exogenously expressed Mcu is mitochondrially localized and
            increases mitochondrial Ca(2+) levels following NMDA receptor
            activation, leading to increased mitochondrial membrane
            depolarization and excitotoxic cell death.
REFERENCE   7  (bases 1 to 2872)
  AUTHORS   Plovanich,M., Bogorad,R.L., Sancak,Y., Kamer,K.J., Strittmatter,L.,
            Li,A.A., Girgis,H.S., Kuchimanchi,S., De Groot,J., Speciner,L.,
            Taneja,N., Oshea,J., Koteliansky,V. and Mootha,V.K.
  TITLE     MICU2, a paralog of MICU1, resides within the mitochondrial
            uniporter complex to regulate calcium handling
  JOURNAL   PLoS One 8 (2), e55785 (2013)
   PUBMED   23409044
REFERENCE   8  (bases 1 to 2872)
  AUTHORS   Tarasov,A.I., Semplici,F., Ravier,M.A., Bellomo,E.A., Pullen,T.J.,
            Gilon,P., Sekler,I., Rizzuto,R. and Rutter,G.A.
  TITLE     The mitochondrial Ca2+ uniporter MCU is essential for
            glucose-induced ATP increases in pancreatic beta-cells
  JOURNAL   PLoS One 7 (7), e39722 (2012)
   PUBMED   22829870
  REMARK    GeneRIF: Data show that the recently-identified mitochondrial
            Ca(2+) uniporter, MCU, is required for depolarisation-induced
            mitochondrial Ca(2+) increases, and for a sustained increase in
            cytosolic ATP/ADP ratio.
REFERENCE   9  (bases 1 to 2872)
  AUTHORS   De Stefani,D., Raffaello,A., Teardo,E., Szabo,I. and Rizzuto,R.
  TITLE     A forty-kilodalton protein of the inner membrane is the
            mitochondrial calcium uniporter
  JOURNAL   Nature 476 (7360), 336-340 (2011)
   PUBMED   21685888
  REMARK    GeneRIF: data demonstrate that the 40-kDa protein identified is the
            channel responsible for ruthenium-red-sensitive mitochondrial
            Ca(2+) uptake, thus providing a molecular basis for this process of
            utmost physiological and pathological relevance
            GeneRIF: Includes expression analysis of the mouse Ccdc109a (MCU)
            gene.
            Publication Status: Online-Only
REFERENCE   10 (bases 1 to 2872)
  AUTHORS   Baughman,J.M., Perocchi,F., Girgis,H.S., Plovanich,M.,
            Belcher-Timme,C.A., Sancak,Y., Bao,X.R., Strittmatter,L.,
            Goldberger,O., Bogorad,R.L., Koteliansky,V. and Mootha,V.K.
  TITLE     Integrative genomics identifies MCU as an essential component of
            the mitochondrial calcium uniporter
  JOURNAL   Nature 476 (7360), 341-345 (2011)
   PUBMED   21685886
  REMARK    GeneRIF: genomic, physiological, biochemical and pharmacological
            data firmly establish MCU as an essential component of the
            mitochondrial Ca(2+) uniporter
            GeneRIF: In vivo silencing of CCDC109a (MCU) in mouse liver
            abolishes mitochondrial calcium uptake without perturbing gross
            liver anatomy, mitochondrial oxygen consumption or membrane
            potential.
            Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK144727.1, AK161322.1 and AC022699.11.
            
            On Dec 21, 2011 this sequence version replaced NM_001033259.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13948564.1376494.1,
                                           SRR13422600.1056780.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-694               AK144727.1         2-695
            695-1259            AK161322.1         517-1081
            1260-2872           AC022699.11        84101-85713
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 29.55 cM"
     gene            1..2872
                     /gene="Mcu"
                     /gene_synonym="2010012O16Rik; C10orf42; Ccdc109a;
                     D130073L02Rik; Gm64"
                     /note="mitochondrial calcium uniporter"
                     /db_xref="GeneID:215999"
                     /db_xref="MGI:MGI:3026965"
     exon            1..169
                     /gene="Mcu"
                     /gene_synonym="2010012O16Rik; C10orf42; Ccdc109a;
                     D130073L02Rik; Gm64"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..1075
                     /gene="Mcu"
                     /gene_synonym="2010012O16Rik; C10orf42; Ccdc109a;
                     D130073L02Rik; Gm64"
                     /note="calcium uniporter protein, mitochondrial;
                     coiled-coil domain containing 109A"
                     /codon_start=1
                     /product="calcium uniporter protein, mitochondrial
                     precursor"
                     /protein_id="NP_001028431.2"
                     /db_xref="CCDS:CCDS23866.2"
                     /db_xref="GeneID:215999"
                     /db_xref="MGI:MGI:3026965"
                     /translation="MAAAAGRSLLLLLCSRGGGGGAGGCGALTAGCFPGLGVSRHRPH
                     QQHRTAHQRPASWQSVGAAYCSTVVPSDDVTVVYQNGLPVISVRLPSRRERCQFTLKP
                     ISDSVGVFLRQLQEEDRGIDRVAIYSPDGVRVAASTGIDLLLLDDFKLVINDLTYHVR
                     PPKRDLLSHEDAATLNDVKTLVQQLYTTLCIEQHQLNKERELVERLEDLKQQLAPLEK
                     VRIEISRKAEKRTTLVLWGGLAYMATQFGILARLTWWEYSWDIMEPVTYFITYGSAMA
                     MYAYFVMTRQEYVYPEARDRQYLLFFHKGAKKSRFDLEKYNQLKDAIAQAEMDLKRLR
                     DPLQVHLPLRQIGEKE"
     exon            170..239
                     /gene="Mcu"
                     /gene_synonym="2010012O16Rik; C10orf42; Ccdc109a;
                     D130073L02Rik; Gm64"
                     /inference="alignment:Splign:2.1.0"
     exon            240..410
                     /gene="Mcu"
                     /gene_synonym="2010012O16Rik; C10orf42; Ccdc109a;
                     D130073L02Rik; Gm64"
                     /inference="alignment:Splign:2.1.0"
     exon            411..515
                     /gene="Mcu"
                     /gene_synonym="2010012O16Rik; C10orf42; Ccdc109a;
                     D130073L02Rik; Gm64"
                     /inference="alignment:Splign:2.1.0"
     exon            516..676
                     /gene="Mcu"
                     /gene_synonym="2010012O16Rik; C10orf42; Ccdc109a;
                     D130073L02Rik; Gm64"
                     /inference="alignment:Splign:2.1.0"
     exon            677..880
                     /gene="Mcu"
                     /gene_synonym="2010012O16Rik; C10orf42; Ccdc109a;
                     D130073L02Rik; Gm64"
                     /inference="alignment:Splign:2.1.0"
     exon            881..997
                     /gene="Mcu"
                     /gene_synonym="2010012O16Rik; C10orf42; Ccdc109a;
                     D130073L02Rik; Gm64"
                     /inference="alignment:Splign:2.1.0"
     exon            998..2872
                     /gene="Mcu"
                     /gene_synonym="2010012O16Rik; C10orf42; Ccdc109a;
                     D130073L02Rik; Gm64"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2848..2853
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mcu"
                     /gene_synonym="2010012O16Rik; C10orf42; Ccdc109a;
                     D130073L02Rik; Gm64"
                     /note="hexamer: AATAAA"
     polyA_site      2872
                     /gene="Mcu"
                     /gene_synonym="2010012O16Rik; C10orf42; Ccdc109a;
                     D130073L02Rik; Gm64"
                     /note="major polyA site"
ORIGIN      
        1 cagcgccgtt tccagttgag agatggcggc cgccgcaggt agatcgctcc tgctgctgct
       61 ctgctcccgg ggcggcggcg ggggcgccgg cggctgcgga gcgctgactg ccggctgctt
      121 tcccgggctg ggcgtcagcc gccaccggcc gcaccagcag caccggacgg cacaccagag
      181 gccggcttcc tggcagagcg tgggagccgc atattgcagt acggttgtgc cctctgatga
      241 cgtgacggtg gtttaccaaa atggattacc ggtgatatct gtgaggctac cctctcgacg
      301 cgagcgctgc caattcacac tcaagcctat ctctgactca gtcggcgtgt ttttacgaca
      361 actgcaagag gaggatcggg gaatcgaccg ggtcgccatc tactcaccag atggcgttcg
      421 agtcgctgcc tccacgggga tagacctcct gctcctcgat gactttaagc ttgtcattaa
      481 tgacttaaca taccacgtac ggccaccaaa gagagacctc ctaagccatg aagatgcagc
      541 gacgctgaac gacgtgaaga ccctggtcca gcagctgtac accacactgt gcattgagca
      601 gcatcagctt aacaaagagc gggagctcgt ggagaggtta gaggacctca agcagcagct
      661 ggcccccctg gagaaggtac gaattgaaat tagcagaaaa gcagagaaga ggaccactct
      721 ggtgctgtgg ggaggcctgg cctacatggc cacccagttt ggcattctgg cccggctcac
      781 ctggtgggag tactcgtggg acatcatgga gcccgtcacc tacttcatca cgtacggaag
      841 cgccatggcc atgtatgcgt attttgtaat gacgcgccag gaatatgttt atccagaagc
      901 cagagacaga caatacttat tatttttcca taaaggagcc aaaaagtcac gtttcgacct
      961 agagaaatac aatcagctca aggatgcaat tgctcaggca gaaatggatc ttaagagact
     1021 gagagaccca ttacaagtac acctgcccct ccgacagatc ggagaaaagg aatgatccga
     1081 gatgaccgtg aatcccggca gagagtgcgc ctgtttgtaa ctcacgccgt tttaattaaa
     1141 gcatggcagg tggaggctgg ggtagagggt ttttaccttt aattctaaaa acaaaacaca
     1201 aaaaggatct tagggaataa agggatctta aatgctgagg atccagcatc atggaatcca
     1261 agtgcaaagg gcttagcgag tcttgtctta agcatctcca tttcggatga gagtgaagac
     1321 tccgtcgcag ctgggcggag gatgttgggt gtttgcttct tgcatctttg cagggtctgc
     1381 ggaacactaa cacaccagca ccccactcag ctccaggttt ttgtttttgt tttttaattt
     1441 aaagtttctt atttttgaca taggaataaa taagtgtata agaaaatcaa atcttctgat
     1501 tcatgaatgg cagagtctgg gccagtgcgg ctagccttca ttttaagctg gacctggcct
     1561 tcattttaag ctggacccgg tctctgcaaa ggtaccagaa tctctcggcc ctgcacccct
     1621 tttctgacca cctacctacc tgcagatcct ggttggttgg tcacactgct tttaaccaca
     1681 taatcattgg aattaagtta tgattgtttg atgattgaca cttggattat ctcttaatga
     1741 gatctcatgt ctatctcccc ggggctctga atcaatgtct agaagtcact ggcaatactg
     1801 catgagggtt tttgttgtat ttttttcctt tttttttttc ttttggtttt tttttttttt
     1861 tttttttact aattagtctt gagaggagga aaaattcctt cctttatata ctgatcccat
     1921 tgataacccc cacttcctgc acaccacgga tcagaaggcc cgtgctcatc catattacca
     1981 aaagaggagg atgtaaggtt tacagatcgg ctaaaaatca ggctccgagt ctatttcaga
     2041 ccaagagact ctgggttaat ctaacacaca cgtcatcatg tgctctcaca actccaccac
     2101 cccagctgct ggaaatcaag ggcggatctc aaaggctgca gctatctttt taccaggaat
     2161 atcttcctat ggcttccttt agcctgtctt ctccaccccg tttattttta tcttaaacag
     2221 agagacagga gaattactct tacactctga tagagtgttt tctaccatct tcccagatgg
     2281 tccctgcgct cgaagtcaaa gcactctgcc tagtggcctg tttttaactg ttctggggac
     2341 aggatggcaa ggaggacggg ggcttgccac acgaccgatt gattgggtgt ttggttttgg
     2401 tttgcttcct tcccatgatt caccccgcct catgggacaa ggagtttctt tttcctcctc
     2461 ctttggggat cattgtatat gaaagaaaac ctttgaatga caaacccaga ctctaggtgc
     2521 cttgcagagg ttgaaggcca gccacgccac tgcttccacc accagccgcc actggcctgc
     2581 cagagaaagg atgcatgtct cggtccccag cccgcctcct ccttccttcc tgtccttcaa
     2641 cctctcccct gtttcttttc cttgtgcaga ttatttttaa gccatctatc ctgtttgtgt
     2701 gcagggttct taagaaggca tggcacagtg caatgagcaa atcctctgag tgtgggaggc
     2761 ggggggtggg cgagcaagat gttggacacg tgtacaatcc tgtatcattt atgaaatatg
     2821 tataaaaagc aatgtacctt ccggaacaat aaatacttat tcaatttttg aa
//
