LOCUS       NM_019434               6470 bp    mRNA    linear   ROD 06-JUN-2024
DEFINITION  Mus musculus minichromosome maintenance complex component 3
            associated protein (Mcm3ap), mRNA.
ACCESSION   NM_019434
VERSION     NM_019434.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Eid,M.M.A., Shimoda,M., Singh,S.K., Almofty,S.A., Pham,P.,
            Goodman,M.F., Maeda,K. and Sakaguchi,N.
  TITLE     Integrity of immunoglobulin variable regions is supported by GANP
            during AID-induced somatic hypermutation in germinal center B cells
  JOURNAL   Int Immunol 29 (5), 211-220 (2017)
   PUBMED   28541550
  REMARK    GeneRIF: GANP works to maintain antibody structure by reducing
            drastic changes in the IgV framework regions in affinity maturation
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   Kuwahara,K., Yamamoto-Ibusuki,M., Zhang,Z., Phimsen,S., Gondo,N.,
            Yamashita,H., Takeo,T., Nakagata,N., Yamashita,D., Fukushima,Y.,
            Yamamoto,Y., Iwata,H., Saya,H., Kondo,E., Matsuo,K., Takeya,M.,
            Iwase,H. and Sakaguchi,N.
  TITLE     GANP protein encoded on human chromosome 21/mouse chromosome 10 is
            associated with resistance to mammary tumor development
  JOURNAL   Cancer Sci 107 (4), 469-477 (2016)
   PUBMED   26749495
  REMARK    GeneRIF: These results indicated that the GANP protein is
            associated with breast cancer resistance.
REFERENCE   3  (bases 1 to 6470)
  AUTHORS   Eid,M.M., Maeda,K., Almofty,S.A., Singh,S.K., Shimoda,M. and
            Sakaguchi,N.
  TITLE     GANP regulates the choice of DNA repair pathway by DNA-PKcs
            interaction in AID-dependent IgV region diversification
  JOURNAL   J Immunol 192 (12), 5529-5539 (2014)
   PUBMED   24808370
  REMARK    GeneRIF: GANP plays a positive role in IgV region diversification
            of DT40 B cells in a nonhomologous end joining-proficient state.
            DNA-PKcs physically interacts with GANP, and this interaction is
            dissociated by dsDNA breaks
REFERENCE   4  (bases 1 to 6470)
  AUTHORS   Singh,S.K., Maeda,K., Eid,M.M., Almofty,S.A., Ono,M., Pham,P.,
            Goodman,M.F. and Sakaguchi,N.
  TITLE     GANP regulates recruitment of AID to immunoglobulin variable
            regions by modulating transcription and nucleosome occupancy
  JOURNAL   Nat Commun 4, 1830 (2013)
   PUBMED   23652018
  REMARK    GeneRIF: GANP-mediated chromatin modification promotes
            transcription complex recruitment and positioning at immunoglobulin
            variable loci to favour AID targeting.
REFERENCE   5  (bases 1 to 6470)
  AUTHORS   Maeda,K., Singh,S.K., Eda,K., Kitabatake,M., Pham,P., Goodman,M.F.
            and Sakaguchi,N.
  TITLE     GANP-mediated recruitment of activation-induced cytidine deaminase
            to cell nuclei and to immunoglobulin variable region DNA
  JOURNAL   J Biol Chem 285 (31), 23945-23953 (2010)
   PUBMED   20507984
  REMARK    GeneRIF: GANP may serve as an essential link required to transport
            AID to B-cell nuclei and to target AID to actively transcribed IgV
            regions
REFERENCE   6  (bases 1 to 6470)
  AUTHORS   Kuwahara,K., Fujimura,S., Takahashi,Y., Nakagata,N., Takemori,T.,
            Aizawa,S. and Sakaguchi,N.
  TITLE     Germinal center-associated nuclear protein contributes to affinity
            maturation of B cell antigen receptor in T cell-dependent responses
  JOURNAL   Proc Natl Acad Sci U S A 101 (4), 1010-1015 (2004)
   PUBMED   14715907
  REMARK    GeneRIF: role in generation or maintenance of B cells with
            high-affinity B cell antigen receptors during the maturation in
            germinal centers
REFERENCE   7  (bases 1 to 6470)
  AUTHORS   Fujimura,S., Kuwahara,K., Ezaki,T., Tomita,K., Hirose,S. and
            Sakaguchi,N.
  TITLE     Spontaneous increase of plasma-like cells with high GANP expression
            in the extrafollicular region of lymphoid organs of
            autoimmune-prone mice
  JOURNAL   J Autoimmun 20 (4), 291-301 (2003)
   PUBMED   12791315
  REMARK    GeneRIF: GANP(hi)plasma-like cells appear in lymph nodes of
            autoimmune mice during aging
REFERENCE   8  (bases 1 to 6470)
  AUTHORS   Kono,Y., Maeda,K., Kuwahara,K., Yamamoto,H., Miyamoto,E.,
            Yonezawa,K., Takagi,K. and Sakaguchi,N.
  TITLE     MCM3-binding GANP DNA-primase is associated with a novel
            phosphatase component G5PR
  JOURNAL   Genes Cells 7 (8), 821-834 (2002)
   PUBMED   12167160
  REMARK    GeneRIF: interaction with MCM3 is associated with a novel
            phosphatase component G5PR
REFERENCE   9  (bases 1 to 6470)
  AUTHORS   EL-Gazzar,M.A., Maeda,K., Nomiyama,H., Nakao,M., Kuwahara,K. and
            Sakaguchi,N.
  TITLE     PU.1 is involved in the regulation of B lineage-associated and
            developmental stage-dependent expression of the germinal
            center-associated DNA primase GANP
  JOURNAL   J Biol Chem 276 (51), 48000-48008 (2001)
   PUBMED   11641399
REFERENCE   10 (bases 1 to 6470)
  AUTHORS   Kuwahara,K., Yoshida,M., Kondo,E., Sakata,A., Watanabe,Y., Abe,E.,
            Kouno,Y., Tomiyasu,S., Fujimura,S., Tokuhisa,T., Kimura,H.,
            Ezaki,T. and Sakaguchi,N.
  TITLE     A novel nuclear phosphoprotein, GANP, is up-regulated in
            centrocytes of the germinal center and associated with MCM3, a
            protein essential for DNA replication
  JOURNAL   Blood 95 (7), 2321-2328 (2000)
   PUBMED   10733502
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY004673.1, BC052452.1 and BC067414.1.
            
            On Jun 26, 2018 this sequence version replaced NM_019434.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ006590.1, AK122313.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-69                BY004673.1         13-81
            70-6452             BC052452.1         1-6383
            6453-6470           BC067414.1         5319-5336
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 38.88 cM"
     gene            1..6470
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /note="minichromosome maintenance complex component 3
                     associated protein"
                     /db_xref="GeneID:54387"
                     /db_xref="MGI:MGI:1930089"
     exon            1..313
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            314..1617
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     CDS             420..6335
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /EC_number="2.3.1.48"
                     /note="nuclear protein GANP; minichromosome maintenance
                     deficient 3-associated protein; 80 kDa MCM3-associated
                     protein; GC-associated DNA primase"
                     /codon_start=1
                     /product="germinal-center associated nuclear protein"
                     /protein_id="NP_062307.2"
                     /db_xref="CCDS:CCDS23947.1"
                     /db_xref="GeneID:54387"
                     /db_xref="MGI:MGI:1930089"
                     /translation="MHPVNPFGGQQPSAFAVSSSTTGTYQTKSPFRFGQPSLFGQNST
                     PSKSLAFSQVPSFATPSGGSHSSSLPAFGLTQTSSVGLFSSLESTPSFAATSSSSVPG
                     NTAFSFKSTSSVGVFPSGATFGPETGEVAGSGFRKTEFKFKPLENAVFKPIPGPESEP
                     EKTQSQISSGFFTFSHPVGSGSGGLTPFSFPQVTNSSVTSSSFIFSKPVTSNTPAFAS
                     PLSNQNVEEEKRVSTSAFGSSNSSFSTFPTASPGSLGEPFPANKPSLRQGCEEAISQV
                     EPLPTLMKGLKRKEDQDRSPRRHCHEAAEDPDPLSRGDHPPDKRPVRLNRPRGGTLFG
                     RTIQEVFKSNKEAGRLGSKESKESGFAEPGESDHAAVPGGSQSTMVPSRLPAVTKEEE
                     ESRDEKEDSLRGKSVRQSKRREEWIYSLGGVSSLELTAIQCKNIPDYLNDRAILEKHF
                     SKIAKVQRVFTRRSKKLAVIHFFDHASAALARKKGKGLHKDVVIFWHKKKISPSKKLF
                     PLKEKLGESEASQGIEDSPFQHSPLSKPIVRPAAGSLLSKSSPVKKPSLLKMHQFEAD
                     PFDSGSEGSEGLGSCVSSLSTLIGTVADTSEEKYRLLDQRDRIMRQARVKRTDLDKAR
                     AFVGTCPDMCPEKERYLRETRSQLSVFEVVPGTDQVDHAAAVKEYSRSSADQEEPLPH
                     ELRPSAVLSRTMDYLVTQIMDQKEGSLRDWYDFVWNRTRGIRKDITQQHLCDPLTVSL
                     IEKCTRFHIHCAHFMCEEPMSSFDAKINNENMTKCLQSLKEMYQDLRNKGVFCASEAE
                     FQGYNVLLNLNKGDILREVQQFHPDVRNSPEVNFAVQAFAALNSNNFVRFFKLVQSAS
                     YLNACLLHCYFNQIRKDALRALNVAYTVSTQRSTVFPLDGVVRMLLFRDSEEATNFLN
                     YHGLTVADGCVELNRSAFLEPEGLCKARKSVFIGRKLTVSVGEVVNGGPLPPVPRHTP
                     VCSFNSQNKYVGESLATELPISTQRAGGDPAGGGRGEDCEAEVDVPTLAVLPQPPPAS
                     SATPALHVQPLAPAAAPSLLQASTQPEVLLPKPAPVYSDSDLVQVVDELIQEALQVDC
                     EEVSSAGAAYVAAALGVSNAAVEDLITAATTGILRHVAAEEVSMERQRLEEEKQRAEE
                     ERLKQERELMLTQLSEGLAAELTELTVTECVWETCSQELQSAVEIDQKVRVARCCEAV
                     CAHLVDLFLAEEIFQTAKETLQELQCFCKYLQRWREAVAARKKFRRQMRAFPAAPCCV
                     DVNDRLQALVPSAECPITEENLAKGLLDLGHAGKVGVSCTRLRRLRNKTAHQIKVQHF
                     HQQLLRNAAWAPLDLPSIVSEHLPMKQKRRFWKLVLVLPDVEEQTPESPGRILENWLK
                     VKFTGDDSMVGDIGDNAGDIQTLSVFNTLSSKGDQTVSVNVCIKVAHGTLSDSALDAV
                     ETQKDLLGTSGLMLLLPPKVKSEEVAEEELSWLSALLQLKQLLQAKPFQPALPLVVLV
                     PSSRGDSAGRAVEDGLMLQDLVSAKLISDYIVVEIPDSVNDLQGTVKVSGAVQWLISR
                     CPQALDLCCQTLVQYVEDGISREFSRRFFHDRRERRLASLPSQEPSTIIELFNSVLQF
                     LASVVSSEQLCDISWPVMEFAEVGGSQLLPHLHWNSPEHLAWLKQAVLGFQLPQMDLP
                     PPGAPWLPVCSMVIQYTSQIPSSSQTQPVLQSQVENLLCRTYQKWKNKSLSPGQELGP
                     SVAEIPWDDIITLCINHKLRDWTPPRLPVTLEALSEDGQICVYFFKNLLRKYHVPLSW
                     EQARMQTQRELQLSHGRSGMRSIHPPTSTFPTPLLHVHQKGKKKEESGREGSLSTEDL
                     LRGASAEELLAQSLSSSLLEEKEENKRFEDQLQQWLSQDSQAFTESTRLPLYLPQTLV
                     SFPDSIKTQTMVKTSTSPQNSGTGKQLRFSEASGSSLTEKLKLLERLIQSSRAEEAAS
                     ELHLSALLEMVDM"
     exon            1618..1844
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..1923
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            1924..2065
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            2066..2256
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            2257..2396
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            2397..2594
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            2595..2863
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            2864..3026
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            3027..3187
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            3188..3429
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            3430..3629
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            3630..3730
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            3731..3862
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            3863..3976
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            3977..4129
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            4130..4327
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            4328..4396
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            4397..4531
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            4532..4685
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            4686..4944
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            4945..5042
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            5043..5433
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            5434..5691
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            5692..5821
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            5822..6025
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            6026..6176
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     exon            6177..6454
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6430..6435
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /note="hexamer: AATAAA"
     polyA_site      6452
                     /gene="Mcm3ap"
                     /gene_synonym="GANP"
                     /note="major polyA site"
ORIGIN      
        1 agcgcacacc caagagcccg ctccagtggc cggtttgttg cggtgcggtg ggcccggtag
       61 aggctgcacg cagactgtgg gcgagcacaa gcgctggcga cagtggccgt atctggcgga
      121 cttgctcctc cctccgcggc ctccgctgtc ccttgtgtct ttgccgagtt gctgaaggcc
      181 ttcactagtc ttcgctcgaa ggcgtctgtt aacctagcgg ccggcttccg gagtgttaag
      241 catcggggat aaaaagctat tatttctaga ccagggcatc gcaagttcga gttaccggga
      301 gaaaaatgag atggtcatcc tgaggatgaa ggagagcttc ccctggcaac agataattta
      361 aagaggagag ctacttgtgt atagtccata tttattgcct tcagataatt ggcttgaaga
      421 tgcacccggt gaaccccttc ggagggcagc agcccagtgc ttttgcggta tcttccagca
      481 ccacgggaac atatcagact aaatcaccat ttcgatttgg ccagccttcc ctttttggac
      541 agaacagcac acccagcaag agcctggcgt tttcacaagt accaagcttt gcaacaccct
      601 ctggaggaag ccattcttcc tccttgccag catttggact cacccaaacc tcaagtgtgg
      661 gactcttctc tagtctcgaa tccacacctt ctttcgcagc tacttcgagt tcctctgtgc
      721 ccggcaatac ggcattcagc tttaagtcaa cctctagtgt tggggttttc ccaagtggcg
      781 ctacttttgg gccagaaacc ggagaagtag caggttctgg ctttcggaag acggaattca
      841 agtttaaacc tctggaaaat gcagtcttca aaccgatacc ggggcctgag tcagagccag
      901 aaaaaaccca gagccagatt tcttctggat tttttacatt ttcccatccc gttggtagcg
      961 ggtctggagg cctgacccct ttttctttcc cacaggtgac aaatagttcg gtgactagct
     1021 caagttttat cttttcgaaa ccagttacta gtaatactcc tgcctttgcc tctcctttgt
     1081 ctaaccaaaa tgtagaagaa gagaagaggg tttctacgtc agcgtttgga agctcaaaca
     1141 gtagcttcag tactttcccc acagcgtcac caggatcttt gggggagccc ttcccagcta
     1201 acaaaccaag cctccgccaa ggatgtgagg aagccatctc ccaggtggag ccacttccca
     1261 ccctcatgaa gggattaaag aggaaagagg accaggatcg ctccccgagg agacattgcc
     1321 acgaggcagc agaagaccct gatcccctgt ccaggggcga ccatccccca gataaacggc
     1381 cagtccgcct caacagaccc cggggaggta ctttgtttgg ccggacaata caggaggtct
     1441 tcaaaagcaa taaagaggca ggccgcctgg gcagcaagga atccaaggag agtggctttg
     1501 cggaacctgg ggaaagtgac cacgcggccg tcccaggagg gagtcagtcc accatggtac
     1561 cttcccgcct tccagctgtg actaaagagg aagaagaaag tagagatgag aaagaagatt
     1621 ctctcagggg aaagtctgtg cgccagagta agcgaaggga agagtggatc tacagcctcg
     1681 ggggcgtgtc ttctttagag ctcacagcca tccagtgcaa gaacatcccc gactacctca
     1741 acgacagagc catcctggag aaacacttca gcaaaatcgc taaagtccag cgggtcttca
     1801 ccagacgcag caagaagctc gccgtgattc attttttcga ccacgcatcg gcagccctgg
     1861 ctaggaagaa ggggaaaggt ctgcataagg acgtggttat cttttggcac aagaagaaaa
     1921 taagtcccag caagaaactc tttcccctga aggagaagct tggtgagagt gaagccagcc
     1981 agggcatcga ggactccccc tttcagcact cgcctctcag caagcccatc gtgaggcctg
     2041 cagccggcag cctcctcagc aaaagctctc cagtgaagaa gccgagtctt ctgaagatgc
     2101 accagtttga ggcggatcct tttgactctg gatctgaggg ctccgagggc cttggttctt
     2161 gcgtgtcatc tcttagcacc ctgataggga ctgtggcaga cacatctgag gagaagtacc
     2221 gccttctgga ccagagagac cgcatcatgc ggcaagctcg agtgaagagg acggacctgg
     2281 acaaagccag ggcatttgtt gggacgtgcc ctgacatgtg tcccgagaag gagcggtact
     2341 tgagggagac ccggagccag ctgagcgtgt ttgaagttgt cccagggact gaccaggtgg
     2401 accatgcagc agccgtgaag gagtacagcc ggtcctctgc agatcaggag gagcccctgc
     2461 cacatgagct gagaccctca gcagttctca gcaggaccat ggactacctg gtgacccaga
     2521 tcatggacca aaaggaaggc agccttcggg attggtatga cttcgtgtgg aaccgcaccc
     2581 ggggtatacg gaaggacata acacagcagc acctctgtga tcccctgacg gtgtctctga
     2641 tcgagaagtg tacccgattt cacattcact gtgcccactt tatgtgtgag gagcctatgt
     2701 cttcctttga tgccaagatc aacaatgaga acatgaccaa gtgtctacag agtctgaagg
     2761 agatgtacca ggacctgagg aacaagggtg ttttttgtgc cagtgaagca gagtttcagg
     2821 gctacaatgt cctgcttaat ctcaacaaag gagacatttt gagagaagtg cagcagttcc
     2881 accctgacgt taggaactcc ccagaggtga acttcgctgt ccaggctttt gctgcattga
     2941 acagcaataa ttttgtgaga tttttcaaac tggttcagtc agcttcttac ctgaatgcgt
     3001 gcctgttaca ctgttacttt aatcagatcc gcaaggatgc cctccgggca ctcaatgttg
     3061 cttatactgt aagcacacag cgctctaccg tcttccccct ggatggtgtc gtccgcatgc
     3121 tgctgttcag agatagtgaa gaggcgacaa acttcctcaa ttaccatggc ctcactgtag
     3181 ctgatggctg tgttgagctg aatcggtcgg cattcttgga accggaggga ttatgcaagg
     3241 ccaggaagtc agtgtttatt ggccggaagc tgacggtgtc agttggggaa gttgtgaatg
     3301 gagggccgtt gccccctgtt cctcgccata cacctgtgtg cagcttcaac tcccagaata
     3361 agtacgttgg agagagcctg gctacggagc tgcccatcag cactcagaga gctggtggag
     3421 acccagcagg tggtggcaga ggagaggact gtgaggcaga ggtggacgtg ccaacattgg
     3481 cggtcctccc acagccgcct cctgcatcct cagccacgcc ggcgcttcat gtccagccac
     3541 tggccccagc cgcagcaccc agccttctcc aggcctccac gcagcctgag gtgctgcttc
     3601 caaagcctgc gcctgtgtac tctgactcgg acctggtaca ggtggtggac gagctcatcc
     3661 aggaggctct gcaagtggac tgtgaggaag tcagctccgc tggggcagcc tacgtagccg
     3721 cagctctggg cgtttccaat gctgctgtgg aggatctgat tactgctgcg accacgggca
     3781 ttctgaggca cgttgccgct gaggaagttt ccatggaaag gcagagacta gaggaagaga
     3841 agcaacgagc tgaggaggaa cggttgaagc aagagagaga actgatgtta actcagctga
     3901 gcgagggtct ggccgcagag ctgacagaac tcacggtgac agagtgtgtg tgggaaacct
     3961 gctctcagga gctacagagt gcagtagaaa tagaccagaa ggtccgtgtg gcccgctgtt
     4021 gtgaagccgt ctgtgcacac ctggtggatt tgtttcttgc tgaggaaatt ttccagactg
     4081 caaaagagac actccaggaa ctccagtgtt tctgcaagta tctacaacgg tggagggagg
     4141 ctgttgcagc tcggaagaaa ttccggcgtc agatgcgggc cttccctgca gcgccatgct
     4201 gtgtggatgt gaatgaccgg ctgcaggcac tagtgcccag cgcagagtgc cccattactg
     4261 aggagaacct ggccaagggt cttttggacc tgggccacgc aggcaaagta ggcgtctcct
     4321 gtaccaggtt gaggcggctt agaaacaaga cagctcacca gataaaggtc cagcacttcc
     4381 accagcagct gctgaggaat gctgcatggg cacctctgga cctgccatcc attgtgtctg
     4441 agcacctccc catgaagcag aagcgaaggt tttggaaact ggtgctggtg ttgcctgatg
     4501 tggaagagca gactccagag agtcctggca gaatactaga aaactggcta aaggtcaaat
     4561 tcacaggaga tgacagcatg gtgggtgaca taggagataa tgctggtgat atccagaccc
     4621 tctcagtctt taatacactt agtagtaaag gggatcaaac agtttctgtc aacgtgtgta
     4681 taaaggtggc tcatggcacc cttagtgaca gtgcccttga tgctgtggag acccagaagg
     4741 acctgttggg aaccagtggg ctcatgctgc tgcttccccc gaaagtgaag agtgaggagg
     4801 tggcagagga ggaactgtcc tggctgtcgg ctttactgca gctcaagcag cttctgcagg
     4861 ccaagccctt ccagcctgcc ctgccgctgg tggtcctcgt gcccagctcc agaggggact
     4921 ccgcggggag ggcagtagag gacggtctga tgttacagga tttggtttca gccaagctga
     4981 tttccgatta cattgttgtt gagattcctg actctgttaa tgatttacaa ggcacagtga
     5041 aggtttctgg agcagtccag tggctgatct cccgatgtcc tcaagcccta gacctttgct
     5101 gccagaccct tgttcagtat gttgaggatg ggatcagccg cgagttcagc cgtcggtttt
     5161 tccacgacag gagagagagg cgcctggcta gcctgccctc ccaggagcct agcaccatta
     5221 ttgagttgtt caacagtgtg ctgcagttcc tggcctctgt ggtatcctct gagcagctgt
     5281 gtgacatctc ctggcctgtc atggaatttg ccgaagtggg aggcagccag ctgcttcctc
     5341 acctgcactg gaactcacca gagcatctag cgtggctgaa acaagctgtg cttgggttcc
     5401 agcttccaca gatggacctt ccacccccag gggccccctg gctccctgtg tgttccatgg
     5461 tcattcagta cacctcccag attcccagct caagccagac acagcctgtc ctccagtccc
     5521 aggtggagaa cctgctgtgc agaacatacc agaagtggaa gaacaagagc ctctctccag
     5581 gccaggagtt ggggccttct gttgccgaga tcccgtggga tgacatcatc accttatgca
     5641 tcaatcataa gctgagggac tggacacccc ccaggctccc tgtcacatta gaggcgctga
     5701 gtgaagatgg tcaaatatgt gtgtattttt tcaaaaacct tttaagaaaa taccacgttc
     5761 ccttgtcatg ggaacaggcc agaatgcaga cgcagcggga actgcagctg agtcatggac
     5821 gttcggggat gaggtccatc catcctccta caagcacttt tcctactcca ttgcttcatg
     5881 tacaccagaa agggaagaaa aaggaagaga gtggccgaga ggggagcctc agtacagagg
     5941 acctgctgcg gggggcttct gcagaagagc tcctggcaca gagtctgtcc agcagtctgc
     6001 tggaagagaa ggaagagaac aagaggtttg aagatcaact tcagcagtgg ttatcgcaag
     6061 actcacaggc attcacagag tcaactcggc ttcctctcta cctccctcag acgctagtgt
     6121 cctttcctga ttctatcaaa actcagacca tggtgaaaac atctacaagt cctcagaatt
     6181 caggaacagg aaagcagttg aggttctcag aggcatccgg ttcatccctg acggaaaagc
     6241 tgaagctcct ggaaaggctg atccagagct caagggcgga agaagcagcc tccgagctgc
     6301 acctctctgc actgctggag atggtggaca tgtagctgtc tgacgggaga cggatctcta
     6361 attcataatg ctttgtctgt attcaattgt gttatagatg ctgttggaaa tgtgactatt
     6421 aattatgcaa ataaactttt tgaatcattc cagaaaaaaa aaaaaaaaaa
//
