LOCUS       NM_001030014            1917 bp    mRNA    linear   ROD 14-JUN-2024
DEFINITION  Mus musculus malonyl CoA:ACP acyltransferase (mitochondrial)
            (Mcat), transcript variant 1, mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_001030014 XM_110101
VERSION     NM_001030014.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Webb,B.D., Nowinski,S.M., Solmonson,A., Ganesh,J., Rodenburg,R.J.,
            Leandro,J., Evans,A., Vu,H.S., Naidich,T.P., Gelb,B.D.,
            DeBerardinis,R.J., Rutter,J. and Houten,S.M.
  TITLE     Recessive pathogenic variants in MCAT cause combined oxidative
            phosphorylation deficiency
  JOURNAL   Elife 12, e68047 (2023)
   PUBMED   36881526
  REMARK    GeneRIF: Recessive pathogenic variants in MCAT cause combined
            oxidative phosphorylation deficiency.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Li,H., Yuan,S., Minegishi,Y., Suga,A., Yoshitake,K., Sheng,X.,
            Ye,J., Smith,S., Bunkoczi,G., Yamamoto,M. and Iwata,T.
  TITLE     Novel mutations in malonyl-CoA-acyl carrier protein transacylase
            provoke autosomal recessive optic neuropathy
  JOURNAL   Hum Mol Genet 29 (3), 444-458 (2020)
   PUBMED   31915829
  REMARK    GeneRIF: Novel mutations in malonyl-CoA-acyl carrier protein
            transacylase provoke autosomal recessive optic neuropathy.
REFERENCE   3  (bases 1 to 1917)
  AUTHORS   Smith,S., Witkowski,A., Moghul,A., Yoshinaga,Y., Nefedov,M., de
            Jong,P., Feng,D., Fong,L., Tu,Y., Hu,Y., Young,S.G., Pham,T.,
            Cheung,C., Katzman,S.M., Brand,M.D., Quinlan,C.L., Fens,M.,
            Kuypers,F., Misquitta,S., Griffey,S.M., Tran,S., Gharib,A.,
            Knudsen,J., Hannibal-Bach,H.K., Wang,G., Larkin,S., Thweatt,J. and
            Pasta,S.
  TITLE     Compromised mitochondrial fatty acid synthesis in transgenic mice
            results in defective protein lipoylation and energy disequilibrium
  JOURNAL   PLoS One 7 (10), e47196 (2012)
   PUBMED   23077570
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL583889.8.
            
            On Jan 10, 2023 this sequence version replaced NM_001030014.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC099494.1, AK135457.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-465               AL583889.8         3903-4367
            466-553             AL583889.8         7017-7104
            554-771             AL583889.8         10303-10520
            772-1917            AL583889.8         11696-12841
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 39.4 cM"
     gene            1..1917
                     /gene="Mcat"
                     /note="malonyl CoA:ACP acyltransferase (mitochondrial)"
                     /db_xref="GeneID:223722"
                     /db_xref="MGI:MGI:2388651"
     exon            1..465
                     /gene="Mcat"
                     /inference="alignment:Splign:2.1.0"
     CDS             49..1194
                     /gene="Mcat"
                     /EC_number="2.3.1.39"
                     /note="isoform 1 is encoded by transcript variant 1;
                     malonyl-CoA-acyl carrier protein transacylase,
                     mitochondrial; MCT; mitochondrial malonyltransferase;
                     [Acyl-carrier-protein] malonyltransferase"
                     /codon_start=1
                     /product="malonyl-CoA-acyl carrier protein transacylase,
                     mitochondrial isoform 1"
                     /protein_id="NP_001025185.1"
                     /db_xref="CCDS:CCDS27704.1"
                     /db_xref="GeneID:223722"
                     /db_xref="MGI:MGI:2388651"
                     /translation="MSARVARAGWAWRSWGRRAASSLREPPPDAVDVAELLRDSSVAE
                     EGAQEAVARRRPPSQCSVLLFPGQGCQAVGMGSGLLHLPRVRQLYEAAHRVLGYDLLE
                     LCLRGPQEDLDRTVHCQPAVFVASLAAVEKLHHLQPAVIDNCVAAAGFSVGEFAALVF
                     AGAMDFSEGLYAVKARAEAMQEASEAVPSGMLSVLGQRQSNFSFACLEAQEHCKSLGI
                     ENPVCQVSNYLFPDCRVISGHLEALQFLRRNSAKYHFRRTKMLPVSGGFHTCLMEPAV
                     DPLMKVLGSINIKKPLVAVHSNVSGQKYTHPQHIRKLLGQQVVSPVKWEQTMHSIYER
                     KKGMEFPSTYEVGPGQQLGSILKCCNRQAWKSYSHVDVMQNIMDPDP"
     exon            466..553
                     /gene="Mcat"
                     /inference="alignment:Splign:2.1.0"
     exon            554..771
                     /gene="Mcat"
                     /inference="alignment:Splign:2.1.0"
     exon            772..1917
                     /gene="Mcat"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1890..1895
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mcat"
                     /note="hexamer: AATAAA"
     polyA_site      1917
                     /gene="Mcat"
                     /note="major polyA site"
ORIGIN      
        1 agtgcgtctc agccgcagcg ataacgacca ccggctctgc gcgtcgcaat gagcgctcgg
       61 gtagctcggg ccgggtgggc ctggcgctcc tggggccgcc gcgccgcctc gagcctccgg
      121 gagccgccgc cggacgccgt ggacgtggcg gagctgctgc gggactccag cgtggccgag
      181 gagggagccc aggaggcggt ggcgcggcgg cggccacctt cacagtgctc cgtgttgctc
      241 ttccccggcc agggctgcca ggcggtcggc atgggcagcg gcctgctcca cctcccgcgt
      301 gtgcggcagc tctacgaggc cgcgcaccgg gtactgggct acgatctgct ggagctgtgc
      361 ctgcgggggc cccaggagga cctggaccgc acagtgcatt gccagcccgc cgtctttgtg
      421 gcttcactgg ccgccgtgga gaagctacat catctgcagc cggcggtcat cgacaactgt
      481 gttgctgcgg ctggcttcag tgtgggagag tttgctgcct tggtatttgc tggagccatg
      541 gatttttctg aaggtctgta tgcggtgaag gcccgtgccg aagctatgca agaagcatcc
      601 gaagccgttc ccagtgggat gctgtctgtc cttggccagc gtcagtccaa cttctccttt
      661 gcctgcttgg aagcccagga gcactgcaag tctttgggta tagagaaccc agtgtgccag
      721 gtgtccaact acctctttcc tgactgcagg gtcatctcag gacaccttga ggcccttcag
      781 tttctccgga ggaattctgc taagtatcac ttccggcgca ccaagatgct gcctgtaagt
      841 ggaggcttcc atacctgcct catggagcca gccgtggatc ccctgatgaa agttctaggc
      901 tcgatcaaca tcaagaagcc actggttgcc gtccattcca atgtcagtgg gcagaagtac
      961 acacatccac aacacatccg aaagctgcta gggcagcagg tggtgtcgcc agtgaaatgg
     1021 gaacagacaa tgcactctat ctacgagcgg aagaaaggca tggagttccc cagcacctac
     1081 gaggtgggcc ccgggcagca gctgggaagc atcctcaagt gctgcaacag gcaggcgtgg
     1141 aagtcctaca gccatgtgga tgtgatgcag aacatcatgg atcctgaccc ctgagggcct
     1201 tgggcttaga atggccccag tgtgagcctc tgtgcctgcc tcttttcctc agacttgagg
     1261 acgatggaca ctgcagtctc agctgtggct caggctccct cagagcatag tctttgagtg
     1321 cctatcctcc aactgacagg acatcgggct gctggggaaa gggcgcctgt gcagacggcc
     1381 tgcccggcca gaatgctgct ctgcctctgc acccaaccat gttcctcggt gaacccagaa
     1441 gggctagcct gggcagtctt agactcatcc tgtgggtatt gtggacacac tgcttggctt
     1501 caagctaggc ctggtgccag aggatcgtct cagctgcctg tgacgtgctc ctttggtctt
     1561 gaatcttgaa tcaaggtgtg gtggccttgt tcttcactca cttggcaggt gggagattga
     1621 gtcccgtgtg ccatacagac ctcagaagca cttattaagt gcctgtgtat gtcctagaat
     1681 gggtggtcct ttgtataaga aactaggaga accgtgtaag gtgtggagtg ggaaccctgt
     1741 ctttcctagg ccagcctgtg ttcattcaca cagagacacc accaacagca ggtcatccat
     1801 gggacagtgt gtctcatact gtgtttatgg cttcctcaga gcagtctgct ccacagcagg
     1861 agtggggcaa tgctcctggg aagacatata ataaacatga gccattctaa gctgcca
//
