LOCUS       NM_001025259            1985 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus myelin basic protein (Mbp), transcript variant 6,
            mRNA.
ACCESSION   NM_001025259
VERSION     NM_001025259.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Sztachera,M., Wendlandt-Stanek,W., Serwa,R.A., Stanaszek,L.,
            Smuszkiewicz,M., Wronka,D. and Piwecka,M.
  TITLE     Interrogation of RNA-bound proteome with XRNAX illuminates
            molecular alterations in the mouse brain affected with
            dysmyelination
  JOURNAL   Cell Rep 44 (1), 115095 (2025)
   PUBMED   39709601
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Xu,X., Fang,M., Chen,L., Huang,H., Dai,Z.M., Yang,J. and Qiu,M.
  TITLE     Nzf2 promotes oligodendrocyte differentiation and regeneration via
            repressing HDAC1-mediated histone deacetylation
  JOURNAL   Sci Adv 10 (50), eadf8405 (2024)
   PUBMED   39671488
REFERENCE   3  (bases 1 to 1985)
  AUTHORS   Zerad,L., Gacem,N., Gayda,F., Day,L., Sinigaglia,K., Richard,L.,
            Parisot,M., Cagnard,N., Mathis,S., Bole-Feysot,C., O'Connell,M.A.,
            Pingault,V., Dambroise,E., Keegan,L.P., Vallat,J.M. and
            Bondurand,N.
  TITLE     Overexpression of Egr1 Transcription Regulator Contributes to
            Schwann Cell Differentiation Defects in Neural Crest-Specific Adar1
            Knockout Mice
  JOURNAL   Cells 13 (23), 1952 (2024)
   PUBMED   39682701
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1985)
  AUTHORS   Bagheri,H., Friedman,H., Hadwen,A., Jarweh,C., Cooper,E., Oprea,L.,
            Guerrier,C., Khadra,A., Collin,A., Cohen-Adad,J., Young,A.,
            Victoriano,G.M., Swire,M., Jarjour,A., Bechler,M.E., Pryce,R.S.,
            Chaurand,P., Cougnaud,L., Vuckovic,D., Wilion,E., Greene,O.,
            Nishiyama,A., Benmamar-Badel,A., Owens,T., Grouza,V., Tuznik,M.,
            Liu,H., Rudko,D.A., Zhang,J., Siminovitch,K.A. and Peterson,A.C.
  TITLE     Myelin basic protein mRNA levels affect myelin sheath dimensions,
            architecture, plasticity, and density of resident glial cells
  JOURNAL   Glia 72 (10), 1893-1914 (2024)
   PUBMED   39023138
  REMARK    GeneRIF: Myelin basic protein mRNA levels affect myelin sheath
            dimensions, architecture, plasticity, and density of resident glial
            cells.
REFERENCE   5  (bases 1 to 1985)
  AUTHORS   MacArthur,I.C., Ma,L., Huang,C.Y., Bhavsar,H., Suzuki,M. and
            Dawlaty,M.M.
  TITLE     Developmental DNA demethylation is a determinant of neural stem
            cell identity and gliogenic competence
  JOURNAL   Sci Adv 10 (35), eado5424 (2024)
   PUBMED   39196941
REFERENCE   6  (bases 1 to 1985)
  AUTHORS   Grima,B., Zelenika,D. and Pessac,B.
  TITLE     A novel transcript overlapping the myelin basic protein gene
  JOURNAL   J Neurochem 59 (6), 2318-2323 (1992)
   PUBMED   1279125
REFERENCE   7  (bases 1 to 1985)
  AUTHORS   Privat,A., Jacque,C., Bourre,J.M., Dupouey,P. and Baumann,N.
  TITLE     Absence of the major dense line in myelin of the mutant mouse
            'shiverer'
  JOURNAL   Neurosci Lett 12 (1), 107-112 (1979)
   PUBMED   460693
REFERENCE   8  (bases 1 to 1985)
  AUTHORS   Dupouey,P., Jacque,C., Bourre,J.M., Cesselin,F., Privat,A. and
            Baumann,N.
  TITLE     Immunochemical studies of myelin basic protein in shiverer mouse
            devoid of major dense line of myelin
  JOURNAL   Neurosci Lett 12 (1), 113-118 (1979)
   PUBMED   88695
REFERENCE   9  (bases 1 to 1985)
  AUTHORS   Bird,T.D., Farrell,D.F. and Sumi,S.M.
  TITLE     Brain lipid composition of the shiverer mouse: (genetic defect in
            myelin development)
  JOURNAL   J Neurochem 31 (1), 387-391 (1978)
   PUBMED   671037
REFERENCE   10 (bases 1 to 1985)
  AUTHORS   Doolittle,D.P. and Schweikart,K.M.
  TITLE     Myelin deficient, a new neurological mutant in the mouse
  JOURNAL   J Hered 68 (5), 331-332 (1977)
   PUBMED   599283
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY245551.1, AK005129.1 and
            AC119259.15.
            
            On May 11, 2006 this sequence version replaced NM_001025259.1.
            
            Summary: The protein encoded by the classic Mbp gene is a major
            constituent of the myelin sheath of oligodendrocytes and Schwann
            cells in the nervous system. However, Mbp-related transcripts are
            also present in the bone marrow and the immune system. These mRNAs
            arise from the long Mbp gene (otherwise called 'Golli-Mbp') that
            contains 3 additional exons located upstream of the classic Mbp
            exons. Alternative splicing from the Golli and the Mbp
            transcription start sites gives rise to 2 sets of Mbp-related
            transcripts and gene products. The Golli mRNAs contain 3 exons
            unique to Golli-Mbp, spliced in-frame to 1 or more Mbp exons. They
            encode hybrid proteins that have N-terminal Golli aa sequence
            linked to Mbp aa sequence. The second family of transcripts contain
            only Mbp exons and produce the well characterized myelin basic
            proteins. This complex gene structure is conserved among species
            suggesting that the Mbp transcription unit is an integral part of
            the Golli transcription unit and that this arrangement is important
            for the function and/or regulation of these genes. Mutation of the
            Mbp gene is associated with the 'shiverer' and 'myelin deficient'
            phenotypes in mouse. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (6) lacks exons 2 and 6 compared
            to transcript variant 1 and encodes one of the classic Mbp isoforms
            (6) that is missing a 26 aa and a 41 aa segment compared to isoform
            1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BY245551.1, SRR1660817.92464.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-67                BY245551.1         1-67
            68-914              AK005129.1         2-848
            915-1985            AC119259.15        26369-27439         c
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 55.84 cM"
     gene            1..1985
                     /gene="Mbp"
                     /gene_synonym="golli-mbp; Hmbpr; jve; mld; shi"
                     /note="myelin basic protein"
                     /db_xref="GeneID:17196"
                     /db_xref="MGI:MGI:96925"
     exon            1..288
                     /gene="Mbp"
                     /gene_synonym="golli-mbp; Hmbpr; jve; mld; shi"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..504
                     /gene="Mbp"
                     /gene_synonym="golli-mbp; Hmbpr; jve; mld; shi"
                     /note="isoform 6 is encoded by transcript variant 6;
                     shiverer; myelin A1 protein; myelin deficient"
                     /codon_start=1
                     /product="myelin basic protein isoform 6"
                     /protein_id="NP_001020430.1"
                     /db_xref="CCDS:CCDS89290.1"
                     /db_xref="GeneID:17196"
                     /db_xref="MGI:MGI:96925"
                     /translation="MASQKRPSQRSKYLATASTMDHARHGFLPRHRDTGILDSIGRFF
                     SGDRGAPKRGSGKDSHTRTTHYGSLPQKSQHGRTQDENPVVHFFKNIVTPRTPPPSQG
                     KGRGLSLSRFSWGGRDSRSGSPMARR"
     exon            289..390
                     /gene="Mbp"
                     /gene_synonym="golli-mbp; Hmbpr; jve; mld; shi"
                     /inference="alignment:Splign:2.1.0"
     exon            391..426
                     /gene="Mbp"
                     /gene_synonym="golli-mbp; Hmbpr; jve; mld; shi"
                     /inference="alignment:Splign:2.1.0"
     exon            427..459
                     /gene="Mbp"
                     /gene_synonym="golli-mbp; Hmbpr; jve; mld; shi"
                     /inference="alignment:Splign:2.1.0"
     exon            460..1985
                     /gene="Mbp"
                     /gene_synonym="golli-mbp; Hmbpr; jve; mld; shi"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1965..1970
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mbp"
                     /gene_synonym="golli-mbp; Hmbpr; jve; mld; shi"
                     /note="hexamer: AATAAA"
     polyA_site      1977
                     /gene="Mbp"
                     /gene_synonym="golli-mbp; Hmbpr; jve; mld; shi"
                     /note="major polyA site"
ORIGIN      
        1 ttttcccgag atgccccggg aagggaggac aacaccttca aagacaggcc ctcagagtcc
       61 gacgagcttc agaccatcca agaagacccc acagcagctt ccggaggcct ggatgtgatg
      121 gcatcacaga agagaccctc acagcgatcc aagtacctgg ccacagcaag taccatggac
      181 catgccaggc atggcttcct cccaaggcac agagacacgg gcatccttga ctccatcggg
      241 cgcttcttta gcggtgacag gggtgcgccc aagcggggct ctggcaagga ctcacacacg
      301 agaactaccc attatggctc cctgccccag aagtcgcagc acggccggac ccaagatgaa
      361 aacccagtag tccatttctt caagaacatt gtgacacctc gaacaccacc tccatcccaa
      421 gggaagggga gaggcctgtc cctcagcaga tttagctggg gaggaagaga cagccgctct
      481 ggatctccca tggcgagacg ctgagagccc tccccgctca gccttcccga atcctgccct
      541 cggcttctta atataactgc cttaaacttt taattctact tgcaccgatt agctagttag
      601 agcagaccct ctcttaatcc cgtggagccg tgatcgcggt ggggccaggc ccacggcacc
      661 ccgactggtt aaaactattc gtcccttttc gtttgaagat tgagttttct cggggtcttc
      721 tcagccctga cttgttcccc gtgcaccttg ttcgactccg gaggttcagg tgcacggaca
      781 cccttccaag ttcaccccta ctccatcctc agacttttca cggtgaggca cacccctcca
      841 gcttccgtgg gcactgcgga tagacaggca caccgccaag gagccagaga gcatggcgca
      901 ggggactgtg tggtccaggc ttcctttgtt ttcttccccc taaagagctt tgtttttcct
      961 aacaggatca gacagtcttg gagtggctta tacaacgggg gcttgtggta tgtgagcaca
     1021 ggctgggcag ctgtgagagt ccagagtggg gtggccctgg ggacacttcc aggccagcta
     1081 tcccctgcac cccaccagct gatttcgagc gtggcagagg gaaggaaagg ggcgagtggg
     1141 ctgggcaatg gccccaacag gaaacgggga cttaggagaa cacgctggag atatgtgtgg
     1201 ccgccaaatg tcaccatctc tcctcagtgg ctccccagag ctggtgcttt taagaaccct
     1261 gtttcctctc agagcccagg gagagtccaa ggacatggcg catctggaag tgggactgca
     1321 ggagttctct ggtggcctcg tgctgtccct ctggccactt ctcatggtgg ggtggtcagc
     1381 ggcagctcgc catggcagtg cccattggta cacactaacc tcggtggaaa aataaccatt
     1441 ccctgcctcc tagaaaggac tcattcttag ctttaggggg gttcctgtca ctgaatcgag
     1501 tcgctgccct ggatgcaggg ctggcctggg cgacgctcca gggatgagga gctgagaacc
     1561 ccagtctaat aatgtccatc gacacctcct tatccctcta acgtactatg tcttttgatt
     1621 tagcatgcct tctgtagacc ttccaaagag ccccacactg gcaccgtcac ccctaggaag
     1681 gcaggtgatg gttgatgtag cccaatactg catcttgtta atctgttcta actctgagta
     1741 gagtgtgggt ttaagataac accgattaat gtatcgccac aataacgtga gggtaagaga
     1801 aaaagcaggg aagaaatttc cagaaaaaaa ccctccagat tgtcccacgg gagtgtttgc
     1861 cctccagtgt gactgaacga ccttgcccat ggcttcgtcc agacagcgca gctgcagtat
     1921 ggctggacag aagcacctac tattcttgaa tattgaaata aaataataaa cttgcaatga
     1981 tcttt
//
