LOCUS       NM_001126314            1836 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus membrane bound O-acyltransferase domain containing 4
            (Mboat4), transcript variant 1, mRNA.
ACCESSION   NM_001126314 XM_001472220 XM_001476434
VERSION     NM_001126314.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Isokawa,M.
  TITLE     Ghrelin-O-acyltransferase (GOAT) acylates ghrelin in the
            hippocampus
  JOURNAL   Vitam Horm 118, 369-392 (2022)
   PUBMED   35180934
  REMARK    GeneRIF: Ghrelin-O-acyltransferase (GOAT) acylates ghrelin in the
            hippocampus.
REFERENCE   3  (bases 1 to 1836)
  AUTHORS   Murzinski,E.S., Saha,I., Ding,H., Strugatsky,D., Hollibaugh,R.A.,
            Liu,H., Tontonoz,P. and Harran,P.G.
  TITLE     In Search of Small Molecules That Selectively Inhibit MBOAT4
  JOURNAL   Molecules 26 (24), 7599 (2021)
   PUBMED   34946685
  REMARK    GeneRIF: In Search of Small Molecules That Selectively Inhibit
            MBOAT4.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1836)
  AUTHORS   Napolitano,T., Avolio,F., Silvano,S., Forcisi,S., Pfeifer,A.,
            Vieira,A., Navarro-Sanz,S., Friano,M.E., Ayachi,C.,
            Garrido-Utrilla,A., Atlija,J., Hadzic,B., Becam,J.,
            Sousa-De-Veiga,A., Plaisant,M.D., Balaji,S., Pisani,D.F.,
            Mondin,M., Schmitt-Kopplin,P., Amri,E.Z. and Collombat,P.
  TITLE     Gfi1 Loss Protects against Two Models of Induced Diabetes
  JOURNAL   Cells 10 (11), 2805 (2021)
   PUBMED   34831029
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1836)
  AUTHORS   Hornsby,A.K.E., Buntwal,L., Carisi,M.C., Santos,V.V., Johnston,F.,
            Roberts,L.D., Sassi,M., Mequinion,M., Stark,R., Reichenbach,A.,
            Lockie,S.H., Siervo,M., Howell,O., Morgan,A.H., Wells,T.,
            Andrews,Z.B., Burn,D.J. and Davies,J.S.
  TITLE     Unacylated-Ghrelin Impairs Hippocampal Neurogenesis and Memory in
            Mice and Is Altered in Parkinson's Dementia in Humans
  JOURNAL   Cell Rep Med 1 (7), 100120 (2020)
   PUBMED   33103129
  REMARK    GeneRIF: Unacylated-Ghrelin Impairs Hippocampal Neurogenesis and
            Memory in Mice and Is Altered in Parkinson's Dementia in Humans.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1836)
  AUTHORS   Sakata,I., Yang,J., Lee,C.E., Osborne-Lawrence,S., Rovinsky,S.A.,
            Elmquist,J.K. and Zigman,J.M.
  TITLE     Colocalization of ghrelin O-acyltransferase and ghrelin in gastric
            mucosal cells
  JOURNAL   Am J Physiol Endocrinol Metab 297 (1), E134-E141 (2009)
   PUBMED   19401456
  REMARK    GeneRIF: GOAT is the only member of the MBOAT family whose
            expression is highly enriched within gastric ghrelin cells and
            whose whole body distribution mirrors that of ghrelin.
REFERENCE   7  (bases 1 to 1836)
  AUTHORS   Gomez,R., Lago,F., Gomez-Reino,J.J., Dieguez,C. and Gualillo,O.
  TITLE     Expression and modulation of ghrelin O-acyltransferase in cultured
            chondrocytes
  JOURNAL   Arthritis Rheum 60 (6), 1704-1709 (2009)
   PUBMED   19479831
  REMARK    GeneRIF: GOAT mRNA was expressed in murine cartilage explants as
            well as in the cultured murine chondrogenic ATDC-5 cell line.
REFERENCE   8  (bases 1 to 1836)
  AUTHORS   Yang,J., Zhao,T.J., Goldstein,J.L. and Brown,M.S.
  TITLE     Inhibition of ghrelin O-acyltransferase (GOAT) by octanoylated
            pentapeptides
  JOURNAL   Proc Natl Acad Sci U S A 105 (31), 10750-10755 (2008)
   PUBMED   18669668
REFERENCE   9  (bases 1 to 1836)
  AUTHORS   Gutierrez,J.A., Solenberg,P.J., Perkins,D.R., Willency,J.A.,
            Knierman,M.D., Jin,Z., Witcher,D.R., Luo,S., Onyia,J.E. and
            Hale,J.E.
  TITLE     Ghrelin octanoylation mediated by an orphan lipid transferase
  JOURNAL   Proc Natl Acad Sci U S A 105 (17), 6320-6325 (2008)
   PUBMED   18443287
  REMARK    GeneRIF: The identification and characterization of human GOAT, the
            ghrelin O-acyl transferase, is reported.
REFERENCE   10 (bases 1 to 1836)
  AUTHORS   Yang,J., Brown,M.S., Liang,G., Grishin,N.V. and Goldstein,J.L.
  TITLE     Identification of the acyltransferase that octanoylates ghrelin, an
            appetite-stimulating peptide hormone
  JOURNAL   Cell 132 (3), 387-396 (2008)
   PUBMED   18267071
  REMARK    GeneRIF: Study identifies GOAT (Ghrelin O-Acyltransferase), a
            polytopic membrane-bound enzyme that attaches octanoate to serine-3
            of ghrelin; consistent with its function, GOAT mRNA is restricted
            to stomach and intestine, the ghrelin-secreting tissues.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC167537.7.
            
            On Sep 1, 2022 this sequence version replaced NM_001126314.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU721729.1, BC147844.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849376, SAMN00849389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-183               AC167537.7         59913-60095
            184-408             AC167537.7         64953-65177
            409-1836            AC167537.7         68638-70065
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 20.9 cM"
     gene            1..1836
                     /gene="Mboat4"
                     /gene_synonym="Gm171; GOAT"
                     /note="membrane bound O-acyltransferase domain containing
                     4"
                     /db_xref="GeneID:234155"
                     /db_xref="MGI:MGI:2685017"
     exon            1..183
                     /gene="Mboat4"
                     /gene_synonym="Gm171; GOAT"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..1372
                     /gene="Mboat4"
                     /gene_synonym="Gm171; GOAT"
                     /EC_number="2.3.1.-"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ghrelin O-acyltransferase; O-acyltransferase
                     domain-containing protein 4; membrane-bound
                     O-acyltransferase domain-containing protein 4"
                     /codon_start=1
                     /product="ghrelin O-acyltransferase isoform 1"
                     /protein_id="NP_001119786.1"
                     /db_xref="CCDS:CCDS52540.1"
                     /db_xref="GeneID:234155"
                     /db_xref="MGI:MGI:2685017"
                     /translation="MDWLQLFFLHPLSFYQGAAFPFALLFNYLCILDTFSTRARYLFL
                     LAGGGVLAFAAMGPYSLLIFIPALCAVALVSFLSPQEVHRLTFFFQMGWQTLCHLGLH
                     YTEYYLGEPPPVRFYITLSSLMLLTQRVTSLSLDICEGKVEAPRRGIRSKSSFSEHLW
                     DALPHFSYLLFFPALLGGSLCSFRRFQACVQRSSSLYPSISFRALTWRGLQILGLECL
                     KVALRSAVSAGAGLDDCQRLECIYLMWSTAWLFKLTYYSHWILDDSLLHAAGFGAEAG
                     QGPGEEGYVPDVDIWTLETTHRISLFARQWNRSTALWLRRLVFRKSRRWPLLQTFAFS
                     AWWHGLHPGQVFGFLCWSVMVKADYLIHTFANVCIRSWPLRLLYRALTWAHTQLIIAY
                     IMLAVEGRSLSSLCQLCCSYNSLFPVMYGLLLFLLAERKDKRN"
     exon            184..408
                     /gene="Mboat4"
                     /gene_synonym="Gm171; GOAT"
                     /inference="alignment:Splign:2.1.0"
     exon            409..1836
                     /gene="Mboat4"
                     /gene_synonym="Gm171; GOAT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1814..1819
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mboat4"
                     /gene_synonym="Gm171; GOAT"
                     /note="hexamer: ATTAAA"
     polyA_site      1836
                     /gene="Mboat4"
                     /gene_synonym="Gm171; GOAT"
                     /note="major polyA site"
ORIGIN      
        1 gacttccctt ttacaagggc accgcttagg gactctagga aggacagtgg gcctcacatt
       61 caggatggat tggctccagc tcttttttct gcatccttta tcattttatc aaggggctgc
      121 attccccttt gcgcttctgt ttaattatct ctgcatcttg gacacctttt ccacccgggc
      181 caggtacctc tttctcctgg ctggaggagg tgtcctggct tttgctgcca tgggtcccta
      241 ctctctgctc atcttcatcc ctgcgctctg cgctgtggct ctggtctcct tcctcagtcc
      301 acaggaagtc cataggctga ccttcttctt tcagatgggc tggcagaccc tgtgccatct
      361 gggtcttcac tacaccgaat actacctggg tgagcctcca cccgtgaggt tctacatcac
      421 tctttcttcc ctcatgctct tgacgcagag agtcacatcc ctctcactgg acatttgtga
      481 agggaaggtg gaggccccga ggcggggcat caggagcaag agttctttct ctgagcacct
      541 gtgggatgct ctacctcatt tcagctactt gctctttttc cctgctctcc tgggaggctc
      601 cctgtgttcc ttccggaggt ttcaggcttg cgttcaaaga tcaagctctt tgtatccgag
      661 tatctctttt cgggctctga cctggagggg tctgcagatt ctcgggctgg agtgcctcaa
      721 ggtggcgctg aggagcgcgg tgagtgctgg agctggactg gatgactgcc agcggctgga
      781 gtgcatctac ctcatgtggt ccacagcctg gctctttaaa ctcacctatt actcccattg
      841 gatcctggac gactctctcc tccacgcggc gggctttggc gctgaggctg gccaggggcc
      901 tggagaggag ggatacgtcc ccgacgtgga catttggacc ctggaaacta cccacaggat
      961 ctccctgttc gccaggcagt ggaaccgaag cacagctctg tggctcagga ggctcgtctt
     1021 ccggaagagc cggcgctggc ccctgctgca gacatttgcc ttctctgcct ggtggcacgg
     1081 gctccaccca ggtcaggtgt tcggcttcct gtgctggtct gtaatggtga aagccgatta
     1141 tctgattcac acttttgcca acgtatgtat cagatcctgg cccctgcggc tgctttatag
     1201 agccctcact tgggctcata cccaactcat cattgcctac atcatgctgg cggtggaggg
     1261 ccggagcctt tcctctctct gccaactgtg ctgttcttac aacagtctct tccctgtgat
     1321 gtacggtctt ttgctttttc tgttagcgga gagaaaagac aaacgtaact gaaatctttc
     1381 cctgcccttc atctcaccta gccattaaat actgcttagg aaatgccaga agatgccttg
     1441 atgaataatg tatttaaaac tattcatagt gaaagttttt aagtattgga tggataaacc
     1501 tgatggctgg ccacagagat gtgtttctct tctgtggtgg tttgaatagt aatggctccc
     1561 atatgtttat atatttaaat gcttagaggg tgacactact tgaaaggact aggaggtgtg
     1621 gcttcatcgg aggaagtgtg tcactaggct ttgaagtttc aaaagcccag tcctagttta
     1681 gcgtctttcc ctgtagcttg ccgatctgga tatagaactc ttagctcctc cagcaccatg
     1741 tctgcctgca tgccgccatg cttcctgcca tgatgataat ggactaaacc cctggaatta
     1801 taagtcagcc ccaattaaat gatttccttt ataaaa
//
