LOCUS       NM_029924               7055 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus methyl-CpG binding domain protein 5 (Mbd5), transcript
            variant 1, mRNA.
ACCESSION   NM_029924 XM_283719 XM_975065 XM_986540
VERSION     NM_029924.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7055)
  AUTHORS   Tang,F.L., Zhang,X.G., Ke,P.Y., Liu,J., Zhang,Z.J., Hu,D.M., Gu,J.,
            Zhang,H., Guo,H.K., Zang,Q.W., Huang,R., Ma,Y.L. and Kwan,P.
  TITLE     MBD5 regulates NMDA receptor expression and seizures by inhibiting
            Stat1 transcription
  JOURNAL   Neurobiol Dis 181, 106103 (2023)
   PUBMED   36997128
  REMARK    GeneRIF: MBD5 regulates NMDA receptor expression and seizures by
            inhibiting Stat1 transcription.
            Erratum:[Neurobiol Dis. 2023 Jun 15;182:106158. doi:
            10.1016/j.nbd.2023.106158.. PMID: 37244821]
REFERENCE   3  (bases 1 to 7055)
  AUTHORS   Seabra,C.M., Aneichyk,T., Erdin,S., Tai,D.J.C., De Esch,C.E.F.,
            Razaz,P., An,Y., Manavalan,P., Ragavendran,A., Stortchevoi,A.,
            Abad,C., Young,J.I., Maciel,P., Talkowski,M.E. and Gusella,J.F.
  TITLE     Transcriptional consequences of MBD5 disruption in mouse brain and
            CRISPR-derived neurons
  JOURNAL   Mol Autism 11 (1), 45 (2020)
   PUBMED   32503625
  REMARK    GeneRIF: Transcriptional consequences of MBD5 disruption in mouse
            brain and CRISPR-derived neurons.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7055)
  AUTHORS   Farini,D., Cesari,E., Weatheritt,R.J., La Sala,G., Naro,C.,
            Pagliarini,V., Bonvissuto,D., Medici,V., Guerra,M., Di Pietro,C.,
            Rizzo,F.R., Musella,A., Carola,V., Centonze,D., Blencowe,B.J.,
            Marazziti,D. and Sette,C.
  TITLE     A Dynamic Splicing Program Ensures Proper Synaptic Connections in
            the Developing Cerebellum
  JOURNAL   Cell Rep 31 (9), 107703 (2020)
   PUBMED   32492419
REFERENCE   5  (bases 1 to 7055)
  AUTHORS   Walz,K. and Young,J.I.
  TITLE     The methyl binding domain containing protein MBD5 is a
            transcriptional regulator responsible for 2q23.1 deletion syndrome
  JOURNAL   Rare Dis 2 (1), e967151 (2014)
   PUBMED   26942102
  REMARK    Erratum:[doi: 10.15252/emmm.201404044]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7055)
  AUTHORS   Camarena,V., Cao,L., Abad,C., Abrams,A., Toledo,Y., Araki,K.,
            Araki,M., Walz,K. and Young,J.I.
  TITLE     Disruption of Mbd5 in mice causes neuronal functional deficits and
            neurobehavioral abnormalities consistent with 2q23.1 microdeletion
            syndrome
  JOURNAL   EMBO Mol Med 6 (8), 1003-1015 (2014)
   PUBMED   25001218
  REMARK    GeneRIF: This study indicates that the Mbd5(+/) (GT) mouse model
            recapitulates most of the hallmark phenotypes observed in 2q23.1
            deletion carriers including abnormal social behavior, cognitive
            impairment, and motor and craniofacial abnormalities.
REFERENCE   7  (bases 1 to 7055)
  AUTHORS   Tao,Y., Wu,Q., Guo,X., Zhang,Z., Shen,Y. and Wang,F.
  TITLE     MBD5 regulates iron metabolism via methylation-independent genomic
            targeting of Fth1 through KAT2A in mice
  JOURNAL   Br J Haematol 166 (2), 279-291 (2014)
   PUBMED   24750026
  REMARK    GeneRIF: MBD5 regulates iron metabolism via methylation-independent
            genomic targeting of Fth1.
REFERENCE   8  (bases 1 to 7055)
  AUTHORS   Du,Y., Liu,B., Guo,F., Xu,G., Ding,Y., Liu,Y., Sun,X. and Xu,G.
  TITLE     The essential role of Mbd5 in the regulation of somatic growth and
            glucose homeostasis in mice
  JOURNAL   PLoS One 7 (10), e47358 (2012)
   PUBMED   23077600
  REMARK    GeneRIF: These results indicate Mbd5 as an essential factor for
            mouse postnatal growth and maintenance of glucose homeostasis
REFERENCE   9  (bases 1 to 7055)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   10 (bases 1 to 7055)
  AUTHORS   VanBuren,V., Piao,Y., Dudekula,D.B., Qian,Y., Carter,M.G.,
            Martin,P.R., Stagg,C.A., Bassey,U.C., Aiba,K., Hamatani,T.,
            Kargul,G.J., Luo,A.G., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Assembly, verification, and initial annotation of the NIA mouse
            7.4K cDNA clone set
  JOURNAL   Genome Res 12 (12), 1999-2003 (2002)
   PUBMED   12466305
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL845521.9, AL845298.5 and AK173176.1.
            
            On Sep 4, 2008 this sequence version replaced NM_029924.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a). This variant has an
            inferred 5' UTR exon combination based on the mRNA AK155574.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            ##Evidence-Data-START##
            CDS exon combination :: AK173176.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMN00849384, SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-42                AL845521.9         3378-3419
            43-114              AL845521.9         72124-72195
            115-177             AL845298.5         13819-13881
            178-270             AL845298.5         26946-27038
            271-424             AL845298.5         45881-46034
            425-529             AL845298.5         97138-97242
            530-706             AL845298.5         158901-159077
            707-1371            AL845298.5         172771-173435
            1372-1474           AL845298.5         177368-177470
            1475-1655           AL845298.5         178472-178652
            1656-3791           AL845298.5         183406-185541
            3792-7055           AK173176.1         2659-5922
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 28.43 cM"
     gene            1..7055
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /note="methyl-CpG binding domain protein 5"
                     /db_xref="GeneID:109241"
                     /db_xref="MGI:MGI:2138934"
     exon            1..42
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /inference="alignment:Splign:2.1.0"
     exon            43..114
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /inference="alignment:Splign:2.1.0"
     exon            115..177
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /inference="alignment:Splign:2.1.0"
     exon            178..270
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /inference="alignment:Splign:2.1.0"
     exon            271..424
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /inference="alignment:Splign:2.1.0"
     exon            425..529
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /inference="alignment:Splign:2.1.0"
     exon            530..706
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /inference="alignment:Splign:2.1.0"
     exon            707..1371
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /inference="alignment:Splign:2.1.0"
     CDS             1259..6445
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /note="isoform a is encoded by transcript variant 1;
                     methyl-CpG-binding protein MBD5"
                     /codon_start=1
                     /product="methyl-CpG-binding domain protein 5 isoform a"
                     /protein_id="NP_084200.1"
                     /db_xref="CCDS:CCDS38122.1"
                     /db_xref="GeneID:109241"
                     /db_xref="MGI:MGI:2138934"
                     /translation="MNGGKECDGGDKEGGLAAIQVPVGWQRRVDHNGVLYISPSGSLL
                     SCLDQVKTYLLTDGTCKCGLECPLILPKVFNFDPGAAVKQRTAEDVKADDDVTKLCIH
                     KRKIIAVATLHQSMEAPHPSLVLTSPGGGTNATPVVPSRAATPRSVRNKSHEGITNSV
                     MPECKNPFKLMTGSSNAMGRLYMQDLPGSQQQELHPVYPRQRLGSSEHGQKSPFRGSH
                     GGLPSPASSGSQIYGDGSISPRTDPLGSPDVFTRNNPGFHGAPNSSPIHLNRTPLSPP
                     SVMLHGSPVQSSCAMAGRTNIPLSPTLTTKSPVMKKPMCNFSTNMEIPRAMFHHKPPQ
                     GPPPPPPPSCALQKKPLTSEKDPLGILDPIPSKPVNQNPIIINPTSFHSNVHSQVPVM
                     NVSMPPAVVPLPSNLPLPTVKPGHMNHGSHVQRIQHSASTSLSPSPVTSPVHMMGTGI
                     GRIEASPQRSRSSSTSSDHGNFMMPPVGPQATCSGIKVPPRSPRSTIGSPRPSMPSSP
                     STKSDGHHQYKDIPNPLIAGMSNVLNTPSSAAFPTAPAGNGSVKSQPGLLGMPLNQIL
                     NQHNAASFPASSLLSAAAKAQLANQNKLAGNNSSSSNNSGAVASSGNTEGHSTLNTMF
                     PPTANMLLPTGEGQSGRAALRDKLMSQQKDSLRKRKQPPTTVLSLLRQSQMDSSAAPK
                     PGPDLLRKHGQGSFPISSMSQLLQSMSCQSSHLSSNSTPGCGGSNTALPCSANQLHFP
                     DPNMNSTVLQNSLTQSIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNC
                     GMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGGSSLPSSIAIA
                     GSNHPAITKTTSVLQDGVIVTTAAGNPLQSQLPIGSDFPFVGQEHALHFPSNSTANNH
                     LPHPLNPSLLSSLPISLPVNQQHLLNQNLLNILQPSAGEGKSEINLHPLGFLNPNVNA
                     ALAFLSGDVDGQVLQPVHFQLLAALLQNQAQAAAMLPLPSFNLTISDLLQPQNTPLPL
                     LTQMTAPPDRLPSNQSDNSRADTLLGSPLGNPLAGSDTTFNPLLLPAVTGASGLMALN
                     PQLLGGVLNSASANTAIHPEVSIATSSQATTTTTTTSSAVAALTVSTLGGTAVVSMAE
                     TLLNISNNAGNPPGPAKLNSNSMVPQLLNPLLGTGLLGDISSINNSLNNHQLTHLQSL
                     LNSNQMFPPNQQPQQHLLQGHQNLQAFQGQPTVPCPANNNPMACLFQNFQVRMQGDAA
                     LLNKRISTQPGLTTLPENPNLALPHFQDTPCELQPRIDLGQPMKDGLVMGGQGDASVD
                     AIYKAVVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLNLSSAVSAVI
                     HGRNMGGVDHDGRLRNARGARLPKNIDHGKNSSEGDGFECFKSASCHTSRKQWDGEQS
                     PRGERNRWKYEEFLDHPGHIHSSPCHERPNNVSTLPFLAGEQHPILLPPRNCQGDKIL
                     EENFRYNNYKRTMMSFKERLESTVERCTHINGNRPRQSRGFGELLGTAKQDLVLEGQS
                     PGSSNSLESSLVKDYIHYNGDFNAKTINGCVPSPSDAKSISSEDDLRNPDSPSSHELI
                     HYRPRTFNVGDLVWGQIKGLTSWPGKFIREDDVHNSCQQSPEEGKVEPEKLKTLTEGL
                     EAYSRVRKRSRKSGKLNNHLEAAIHEAMSELDKMSGTVHQIPQGDRQMRPPKPKRRKI
                     SR"
     exon            1372..1474
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1655
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..3791
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /inference="alignment:Splign:2.1.0"
     exon            3792..4808
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /inference="alignment:Splign:2.1.0"
     exon            4809..5020
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /inference="alignment:Splign:2.1.0"
     exon            5021..6223
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /inference="alignment:Splign:2.1.0"
     exon            6224..6297
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /inference="alignment:Splign:2.1.0"
     exon            6298..6373
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /inference="alignment:Splign:2.1.0"
     exon            6374..7055
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6943..6948
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /note="hexamer: AATAAA"
     regulatory      6949..6954
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /note="hexamer: AATACA"
     polyA_site      6974
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
     polyA_site      6984
                     /gene="Mbd5"
                     /gene_synonym="9430004D19Rik; C030040A15Rik; Gm1630"
                     /note="major polyA site"
ORIGIN      
        1 gccttccacc aaccagcctc ccgtcagaac gggacatgcg caataacttc tacaagaacc
       61 tgtgtatcgg atcctaacta aatcaaaatg tttgaaatca gaagatcatg atcggtgctt
      121 ttgaatgtgt gtagttttca gaggaattaa aaacagaaga gaaaaggaca actttgggag
      181 agcatcaaag atgtccactt ctgcctccgt aagatagcta ttgcaggctg tgtggtggag
      241 aaaataccta tagaaattaa gacactcaag cgccacagaa atattacaag aaatcaagag
      301 cacagaatat ttccttccat caatcctcag aaccctgaaa catttctaac tccatcaaac
      361 acagagcaca gaggaaggcc caccttggca gatggccaca gaggatgtca acaatttctt
      421 ggagagagaa gcagcactcc cttgctgagc acagtgcccc tggacacttg ccaccattac
      481 actgttggag ctcacgcttg ctatgcttgt cccagtttct ctccagaaga agttggaaac
      541 ctaaccgcct acagaggcca gaatggtgga atgaaacagg aagccgctga agggagctgg
      601 aaagccaagc cctttctgga ggagcagccc ctgctctgtt ccagataacc gtggcgaggc
      661 agaaatgttg actcactctt accgcactca ctgtccagtt tctcaggcta tatcaatgga
      721 attttgaaga catgaaaaca tcagatgaac cagcctgcaa caccttggat tcccattgga
      781 aaatgaagtg gaagcttaaa gcatgtggcc tttaacggcg ggcagtactg gagacattac
      841 ctggctgctg gtgtcaggac agaccggtgt ctagatagtc gaggttattt tagtccagta
      901 tcaaggttca aggcaaaatg tgcaactaaa aacttttcat cccttcctct tctgcttcag
      961 acaggtacca gcataggtga attatgattt tccctggttg agagtgtttg gttttgccta
     1021 tgtagaccat gcctaggaat gaaatacttt tttatttaat gtagattata atggggaaca
     1081 ctttcacaat ggctgatttc aaggacttgg ttccaaactg agcccaagtt tccccgtgta
     1141 cttcgtagtc cgggaaaact gtgctatgct ggcccagttt tgaaggccac catgctctgt
     1201 aatagacgga tatcatctta ttgctgatat ctttggagca cccaagcaga cacagaaaat
     1261 gaatggaggc aaagagtgtg acggaggaga caaggaagga ggtcttgcag ctatccaggt
     1321 tcctgtgggt tggcagcggc gtgtggacca taatggcgtg ctgtacatca gtcccagtgg
     1381 gtctttgtta tcttgcttgg accaggttaa gacatacctg ctcactgacg gaacatgcaa
     1441 gtgtggcttg gaatgtcctc ttattcttcc caaggtgttt aattttgatc ctggagctgc
     1501 tgtgaagcag agaactgcag aagatgttaa ggcagatgat gatgtcacaa agctatgcat
     1561 acataaaaga aagatcattg cagtggccac actccatcaa agcatggagg ccccgcatcc
     1621 ttctctggtg ctcaccagtc ctggaggagg aacaaatgca actccagtag taccttcacg
     1681 ggcggcaact ccaagatcag taagaaacaa gtctcatgaa ggaattacaa attctgtaat
     1741 gccagaatgt aagaatcctt tcaagttaat gactggatca tcaaatgcca tgggaaggct
     1801 atacatgcaa gacctgcctg gaagccagca gcaggagctc caccctgtct acccccgcca
     1861 gagattgggc agtagtgagc atggacagaa atctccattt cgtggcagcc atggaggcct
     1921 gcctagccca gcatcatcag ggtctcagat ctatggagat ggctcaatct ctccaaggac
     1981 tgacccgctc ggaagccctg atgtcttcac aagaaataac cctggttttc atggagctcc
     2041 caattccagc cctattcacc taaataggac tcctctttct ccaccttcag taatgctaca
     2101 tggttctccc gtacagtcat cctgtgcaat ggctggaaga actaatatac ctctttcccc
     2161 aaccttgact acaaagagtc cagtaatgaa aaaaccaatg tgtaattttt caactaatat
     2221 ggaaatacca cgagcaatgt tccaccacaa accaccccaa ggcccacctc cccctcctcc
     2281 accttcttgt gctcttcaga aaaagccatt aacatctgag aaagatccac ttggcattct
     2341 tgaccctatt cctagtaaac cagtgaatca gaaccctatt atcattaatc caaccagttt
     2401 ccattcaaat gtccactctc aggtacctgt gatgaatgta agcatgcctc ctgctgttgt
     2461 tcctttgcca agtaatctcc ctttgccaac tgtaaaacct ggtcatatga atcatgggag
     2521 tcatgtacaa agaattcagc attcagcttc aacctccctg tccccttctc cagtgacatc
     2581 cccagtgcac atgatgggga ctggaattgg aaggattgag gcatcgcccc aaagatcacg
     2641 ctcatcttcc acatcatcag atcatggaaa tttcatgatg ccacctgtag gaccccaggc
     2701 cacttgtagt ggcataaagg ttccacctag gtcaccaagg tcaacaatag gttccccaag
     2761 gccatcaatg ccatcaagcc cttctaccaa gtctgatgga catcatcagt acaaggatat
     2821 ccctaaccca ttaattgctg gaatgagtaa tgtactcaat accccaagca gtgcagcttt
     2881 tcctactgca cctgccggaa atggttctgt aaagagtcag cctggtttgc tgggaatgcc
     2941 tttaaatcag atcttgaacc agcacaatgc tgcctccttt ccagcaagta gtttactctc
     3001 agcagcagcc aaagcacagc tagcaaatca aaacaaactt gctggtaaca acagtagcag
     3061 cagtaacaat tctggagctg ttgccagcag cggcaacact gaaggacaca gcactttaaa
     3121 caccatgttc cctccaactg ccaacatgct tctcccaaca ggtgaaggtc aaagtggtcg
     3181 agcagcacta agagataagt tgatgtctca gcaaaaagac tcattacgga aaagaaagca
     3241 gccacccacc acagtgttga gtttgctcag acagtcccaa atggatagtt ctgcagctcc
     3301 taaacctgga cctgacttgc taaggaagca tgggcagggg tcatttccca tcagttcaat
     3361 gtctcagtta ctacagtcta tgagttgtca aagctctcac ttgagtagca atagtacccc
     3421 gggttgtggg ggatcaaata ctgctttgcc ttgctctgct aaccagctgc attttccaga
     3481 tcctaatatg aattccactg ttcttcagaa ttcactgaca cagagcatac ctttaagagg
     3541 ggaagccgtg cactgccaca atgcaaacac taactttgtt cacagtaaca gtccagtccc
     3601 caaccaccat cttgcaggtt taataaatca gattcaggct agcgggaact gtgggatgct
     3661 cagtcagtcg ggcatggctt taggaaactc attacatccc aatccacctc agtcaagaat
     3721 ttcaacgtcc tccactccag tgataccaaa cagcattgtt agcagctata atcaaacaag
     3781 ttctgaagca ggtggttcaa gcctgccgtc ctccatagct atagctggca gcaaccaccc
     3841 tgccatcaca aagacaacat ctgttctcca agatggtgtc atagtcacca ccgcagctgg
     3901 aaacccactg cagagtcagc tgcccattgg gagtgacttt ccttttgttg gccaagagca
     3961 tgcccttcat tttccatcca acagcaccgc aaacaaccat cttccacacc ccttgaaccc
     4021 cagcctcctc agctctctac ctatctcttt gccagtgaat caacagcatc tcctaaacca
     4081 gaatctatta aatatcctcc agccttcagc aggagaaggc aagtctgaga tcaacctcca
     4141 ccctttaggt tttctcaacc cgaatgtaaa cgctgcttta gctttcctct ccggtgacgt
     4201 ggatgggcag gtattacagc ctgtccactt tcagctctta gcagccctgc ttcagaacca
     4261 agcccaagca gctgccatgc ttcccctgcc gtctttcaat ctgaccatct cagatctttt
     4321 gcaaccgcaa aatacccctt tacccttatt aacacagatg acagccccac cagaccgttt
     4381 gccaagcaat cagtcagaca acagccgagc tgataccctt ttaggcagcc ccctgggaaa
     4441 ccctttagca ggcagtgaca ctacttttaa ccccctgctc ctcccagctg tcactggggc
     4501 ctcaggatta atggccttga atccccagct gttggggggt gtcctgaact cggcatcggc
     4561 caacaccgct attcatccag aggtttccat agcaacctcc tcccaggcaa ccactaccac
     4621 aaccactaca tcatcagcag tggcagcact gactgtctca acacttggtg ggacagcagt
     4681 ggtgtcaatg gcagaaacat tgctgaatat atctaataat gctgggaatc cacctggtcc
     4741 agctaaactc aacagtaact ctatggtgcc acagctactt aaccctctac tggggacagg
     4801 tctgcttggt gatatatcat ccataaacaa ttcattgaat aaccatcaac tgactcatct
     4861 acagtcgcta ctaaacagca atcagatgtt tcctccaaat cagcagccgc agcaacacct
     4921 tctccaggga caccagaatc tccaggcctt ccaaggacaa cccacagtcc cttgcccagc
     4981 taacaataac cccatggctt gtctgtttca gaacttccag gtgaggatgc aaggagatgc
     5041 agctcttcta aataaaagaa taagcactca gcctgggctc acaacacttc ctgaaaaccc
     5101 aaaccttgca cttccacatt tccaagatac accctgtgag ctgcagccaa ggattgacct
     5161 tggtcagcct atgaaggacg gcctcgttat gggtggccaa ggagatgcct ccgtagacgc
     5221 catttacaaa gcagttgtgg atgcagccag caaaggaatg caggttgtca tcaccactgc
     5281 tgtcaacagc acgactcaaa tcagccctat cccagccctg agcgccatga gtgccttcac
     5341 tgcctccatt ggtgacccat taaatctgtc cagtgctgtg agcgctgtca tccacgggcg
     5401 gaacatggga ggagtggatc atgatggcag gctgaggaat gcgagagggg cacggctacc
     5461 caagaacata gaccatggga agaactccag cgaaggagac gggtttgagt gtttcaagtc
     5521 agcgagctgc cacacatcca gaaaacagtg ggatggggag caaagtccca gaggggagcg
     5581 caacaggtgg aagtatgagg agtttttaga tcatccaggc catatccaca gtagtccttg
     5641 ccacgaaagg cccaacaatg tctctacact gccatttcta gctggggaac agcacccgat
     5701 actgttacca ccaagaaact gtcaaggtga taagattctg gaggaaaatt tcaggtataa
     5761 taattacaaa agaactatga tgagttttaa agagagacta gagagtactg tggagagatg
     5821 tacacacatt aacggaaaca gacctcgtca gagtcggggc tttggagagc tgctaggcac
     5881 tgcaaaacaa gacctagttc tagaggggca gtctccgggt tcttcaaata gtttggaaag
     5941 ttctctggtc aaagactaca tccattacaa tggagacttt aatgccaaaa ccattaatgg
     6001 gtgtgtgcct agtccctcag atgctaaaag cattagtagt gaagacgacc tacggaatcc
     6061 agactccccc tcctcacatg agttgataca ctatagacca aggacgttca acgttggcga
     6121 cttggtctgg ggccaaatca aaggactgac ttcctggcct gggaaattca taagagaaga
     6181 cgacgttcac aattcatgcc aacaaagccc cgaggaaggg aaggtggagc ccgagaagtt
     6241 gaagactctc acagaaggtt tagaagccta cagccgcgtc cggaaaagga gcagaaaaag
     6301 tgggaaacta aataaccatt tagaagctgc tattcacgag gccatgagcg aactggacaa
     6361 aatgtctggg actgttcacc aaatcccaca gggtgacaga caaatgaggc cccccaaacc
     6421 caagaggagg aagatctcca gataacagag actgttccac tgacacacag tgttgatcaa
     6481 aggagcatgt acagacacac ctgtatgtgg gtattgatat gaccaccgtt agatcaatct
     6541 gtaatctacg gcagatagct accaccacca cagggcccta aagggaacag caatggagac
     6601 aagcattgat caggaaggaa aatgaatgtc aaaagcaaaa caacaatcaa aatccctgta
     6661 tgaacaaagc ggtaaatggt cacgtgatcg ctgagaacgt tttctattgt actaaaattg
     6721 tgattataag aaatagtcca aatgtttctg gagaattttc agtgttgtat atagaaaata
     6781 cagaatttca tggcgatatt agaaatgtaa atattgtaca atattgtcat gtacaagcat
     6841 acttaatgca cactttatct tttattgtac aaagaaatag tgtacaaaaa tctttggttt
     6901 ctatggagta cacctaccag agactaccag tgtaaagtga taaataaaaa tacaacctaa
     6961 atgcttttct atgataatgt aaaagatgct ggttttgtat ttaatggcag aggaaaggca
     7021 tggtgatgta atacacacat catggcatac actgc
//
