LOCUS       NM_001306143            1563 bp    mRNA    linear   ROD 06-JUN-2024
DEFINITION  Mus musculus methyl-CpG binding domain protein 3 (Mbd3), transcript
            variant 2, mRNA.
ACCESSION   NM_001306143 XM_006513304
VERSION     NM_001306143.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Mendez-Frausto,G., Uresti-Rivera,E.E., Godina-Gonzalez,S.,
            Portales-Perez,D.P., Gonzalez-Amaro,R. and Garcia-Hernandez,M.H.
  TITLE     Expression of mBD4, mBD3 and CRAMP during type II collagen-induced
            arthritis/CIA and their association with inflammation and
            bone-remodeling markers
  JOURNAL   Exp Mol Pathol 123, 104689 (2021)
   PUBMED   34592200
  REMARK    GeneRIF: Expression of mBD4, mBD3 and CRAMP during type II
            collagen-induced arthritis/CIA and their association with
            inflammation and bone-remodeling markers.
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Burgold,T., Barber,M., Kloet,S., Cramard,J., Gharbi,S., Floyd,R.,
            Kinoshita,M., Ralser,M., Vermeulen,M., Reynolds,N., Dietmann,S. and
            Hendrich,B.
  TITLE     The Nucleosome Remodelling and Deacetylation complex suppresses
            transcriptional noise during lineage commitment
  JOURNAL   EMBO J 38 (12) (2019)
   PUBMED   31036553
REFERENCE   3  (bases 1 to 1563)
  AUTHORS   Tsuboi,M., Kishi,Y., Yokozeki,W., Koseki,H., Hirabayashi,Y. and
            Gotoh,Y.
  TITLE     Ubiquitination-Independent Repression of PRC1 Targets during
            Neuronal Fate Restriction in the Developing Mouse Neocortex
  JOURNAL   Dev Cell 47 (6), 758-772 (2018)
   PUBMED   30562514
REFERENCE   4  (bases 1 to 1563)
  AUTHORS   Elsen,G.E., Bedogni,F., Hodge,R.D., Bammler,T.K., MacDonald,J.W.,
            Lindtner,S., Rubenstein,J.L.R. and Hevner,R.F.
  TITLE     The Epigenetic Factor Landscape of Developing Neocortex Is
            Regulated by Transcription Factors Pax6--> Tbr2--> Tbr1
  JOURNAL   Front Neurosci 12, 571 (2018)
   PUBMED   30186101
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1563)
  AUTHORS   Jung,B.P., Purushotham,D. and Eubanks,J.H.
  TITLE     Identification of a murine methyl cytosine phosphate guanine
            binding domain-containing factor 3 (MBD3) promoter segment
            sufficient for driving reporter gene expression in neurons in vitro
            and in vivo
  JOURNAL   Neuroscience 145 (3), 1016-1025 (2007)
   PUBMED   17289276
  REMARK    GeneRIF: A 1072 base pair fragment of the MBD3 promoter is
            sufficient to drive expression in cell lines and primary cultured
            neurons.
REFERENCE   6  (bases 1 to 1563)
  AUTHORS   Hendrich,B., Guy,J., Ramsahoye,B., Wilson,V.A. and Bird,A.
  TITLE     Closely related proteins MBD2 and MBD3 play distinctive but
            interacting roles in mouse development
  JOURNAL   Genes Dev 15 (6), 710-723 (2001)
   PUBMED   11274056
REFERENCE   7  (bases 1 to 1563)
  AUTHORS   Wade,P.A., Gegonne,A., Jones,P.L., Ballestar,E., Aubry,F. and
            Wolffe,A.P.
  TITLE     Mi-2 complex couples DNA methylation to chromatin remodelling and
            histone deacetylation
  JOURNAL   Nat Genet 23 (1), 62-66 (1999)
   PUBMED   10471500
  REMARK    GeneRIF: Component of Mi-2 chromatin remodelling and histone
            deacetylation complex in Xenopus laevis oocytes
REFERENCE   8  (bases 1 to 1563)
  AUTHORS   Hendrich,B., Abbott,C., McQueen,H., Chambers,D., Cross,S. and
            Bird,A.
  TITLE     Genomic structure and chromosomal mapping of the murine and human
            Mbd1, Mbd2, Mbd3, and Mbd4 genes
  JOURNAL   Mamm Genome 10 (9), 906-912 (1999)
   PUBMED   10441743
REFERENCE   9  (bases 1 to 1563)
  AUTHORS   Zhang,Y., Ng,H.H., Erdjument-Bromage,H., Tempst,P., Bird,A. and
            Reinberg,D.
  TITLE     Analysis of the NuRD subunits reveals a histone deacetylase core
            complex and a connection with DNA methylation
  JOURNAL   Genes Dev 13 (15), 1924-1935 (1999)
   PUBMED   10444591
  REMARK    GeneRIF: Component of Nucleosome Remodelling and Histone
            Deacetylation (NuRD) co-repressor complex
REFERENCE   10 (bases 1 to 1563)
  AUTHORS   Hendrich,B. and Bird,A.
  TITLE     Identification and characterization of a family of mammalian
            methyl-CpG binding proteins
  JOURNAL   Mol Cell Biol 18 (11), 6538-6547 (1998)
   PUBMED   9774669
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC152062.6, CK625083.1,
            BQ042794.1 and BP770230.1.
            
            On Apr 16, 2015 this sequence version replaced XM_006513304.2.
            
            Summary: This gene encodes a member of the MBD family of nuclear
            proteins that contain a methyl-CpG binding domain (MBD). The
            encoded protein is a component of the nucleosome remodeling and
            histone deacetylation (NuRD) complex. Deletion of this gene causes
            embryonic lethality in mice. Embryonic stem cells lacking the
            encoded protein are severely compromised in their ability to
            differentiate and fail to commit to developmental lineages in the
            absence of leukemia inhibitory factor. Alternative splicing results
            in multiple transcript variants encoding different isoforms.
            [provided by RefSeq, Apr 2015].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 5' coding region compared to variant 1. It
            encodes isoform 2 which has a shorter and distinct N-terminus,
            compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC038264.1, SRR9219381.345.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-136               AC152062.6         37588-37723
            137-751             CK625083.1         1-615
            752-1116            BQ042794.1         364-728
            1117-1563           BP770230.1         1-447               c
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 39.72 cM"
     gene            1..1563
                     /gene="Mbd3"
                     /note="methyl-CpG binding domain protein 3"
                     /db_xref="GeneID:17192"
                     /db_xref="MGI:MGI:1333812"
     exon            1..314
                     /gene="Mbd3"
                     /inference="alignment:Splign:2.1.0"
     CDS             301..1062
                     /gene="Mbd3"
                     /note="isoform 2 is encoded by transcript variant 2;
                     methyl-CpG-binding protein MBD3"
                     /codon_start=1
                     /product="methyl-CpG-binding domain protein 3 isoform 2"
                     /protein_id="NP_001293072.1"
                     /db_xref="CCDS:CCDS78860.1"
                     /db_xref="GeneID:17192"
                     /db_xref="MGI:MGI:1333812"
                     /translation="MERKSPSGKKFRSKPQLARYLGGSMDLSTFDFRTGKMLMNKMNK
                     SRQRVRYDSSNQVKGKPDLNTALPVRQTASIFKQPVTKITNHPSNKVKSDPQKAVDQP
                     RQLFWEKKLSGLSAFDIAEELVRTMDLPKGLQGVGPGCTDETLLSAIASALHTSTLPI
                     TGQLSAAVEKNPGVWLNTAQPLCKAFMVTDDDIRKQEELVQQVRKRLEEALMADMLAH
                     VEELARDGEAPLDKACAEEEEEEEEEEEEPEPERV"
     exon            315..474
                     /gene="Mbd3"
                     /inference="alignment:Splign:2.1.0"
     exon            475..612
                     /gene="Mbd3"
                     /inference="alignment:Splign:2.1.0"
     exon            613..703
                     /gene="Mbd3"
                     /inference="alignment:Splign:2.1.0"
     exon            704..881
                     /gene="Mbd3"
                     /inference="alignment:Splign:2.1.0"
     exon            882..1067
                     /gene="Mbd3"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1547
                     /gene="Mbd3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1521..1526
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mbd3"
                     /note="hexamer: AATAAA"
     polyA_site      1547
                     /gene="Mbd3"
                     /note="major polyA site"
ORIGIN      
        1 tcctgcgagc gcgaaggggc ggggccgact ccacgcctgc gcagacgagc cccacgcgcg
       61 ggcgggtggg cggagcggcc cccctagcgg gggctgtgaa gcgcggggag ggggccgagc
      121 gggtggcgaa gccggcgcgc gcccggctgg gggcggaggg cggaggcccg tgggacagaa
      181 cagctgcggc gagtggcggc ggcggaggga gccgaatcgg cgacgagccc gggggtcgca
      241 acttgcagaa gcggcggcgg cggcggcatc ggccacggcg ggcggaaaag ccggggcgca
      301 atggagcgga agagccccag cgggaagaag ttccgcagca agccacaact ggcacgttac
      361 ctgggcggat ccatggacct cagcaccttc gacttccgca ccggaaagat gttgatgaac
      421 aagatgaata agagtcgcca gcgtgtgcgc tatgattctt ccaaccaggt caagggcaag
      481 cctgacctga acaccgcgct gcctgtacgg cagactgcat ccatcttcaa gcaaccggtg
      541 accaagatca ccaaccaccc cagcaacaag gtcaagagcg acccgcagaa ggcagtggac
      601 cagccgaggc agcttttctg ggagaagaag ctaagtggat tgagtgcctt tgacattgca
      661 gaagaactgg tcaggaccat ggacttgccc aagggcctgc agggagtggg ccctggctgt
      721 acagatgaga cgctgctgtc agccattgcg agtgctctac acaccagcac cctgcccatt
      781 acaggccagc tctctgcagc cgtggagaag aaccctggtg tgtggctgaa cactgcacag
      841 ccactgtgca aagccttcat ggtgacagat gacgacatca ggaagcagga ggagctggta
      901 cagcaggtac ggaagcgcct ggaggaggca ctgatggccg acatgctagc tcatgtggag
      961 gagcttgccc gagacgggga ggcaccactg gacaaggcct gtgcagagga ggaagaggag
     1021 gaggaagagg aggaggaaga gccggagcca gagcgagtgt agcacaggtg ccctgcccaa
     1081 gtctgggctg cagactgcct tcagccttgc ctggaccagg taggggccag acctgtagga
     1141 ggcagccgtc cacctccttt ccaaagcctc ctgcttccag gtctcagtgc agggagcccc
     1201 tgtggacctt gaactcactt gtccctgcgc tgcctggcag gaagccccac actgaaagca
     1261 gatgagcagt gacccaactg agaggccacc tggacacagt cacctccctg cctccttatc
     1321 ataggacaag gccttgcttg gcaccgagga gctgggagcc gtgttgggtg ctggaggaag
     1381 tttctggaaa cacacctggc tatgcccacc ttatgtccct aaggctatta caggccaggg
     1441 tctggactgc tccggcccac agggctgccc agcctcccca cactgagggt cagcagccca
     1501 ccaggaagtc actttccttc aataaactga tggtaggaac ttgtgaaaaa aaaaaaaaaa
     1561 aaa
//
