LOCUS       NM_001357423            2903 bp    mRNA    linear   ROD 08-FEB-2025
DEFINITION  Mus musculus methyl-CpG binding domain protein 1 (Mbd1), transcript
            variant 4, mRNA.
ACCESSION   NM_001357423 XM_006525704
VERSION     NM_001357423.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Hampton,B.K., Plante,K.S., Whitmore,A.C., Linnertz,C.L.,
            Madden,E.A., Noll,K.E., Boyson,S.P., Parotti,B., Xenakis,J.G.,
            Bell,T.A., Hock,P., Shaw,G.D., de Villena,F.P., Ferris,M.T. and
            Heise,M.T.
  TITLE     Forward genetic screen of homeostatic antibody levels in the
            Collaborative Cross identifies MBD1 as a novel regulator of B cell
            homeostasis
  JOURNAL   PLoS Genet 18 (12), e1010548 (2022)
   PUBMED   36574452
  REMARK    GeneRIF: Forward genetic screen of homeostatic antibody levels in
            the Collaborative Cross identifies MBD1 as a novel regulator of B
            cell homeostasis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2903)
  AUTHORS   Zhang,H., Jiang,J.C., Wu,Y.W., Yu,Y.S., Wang,H.N., Ding,N.Z. and
            Fan,H.Y.
  TITLE     The RNA ligase RNA terminal phosphate cyclase B regulates mRNA
            alternative splicing and is required for mouse oocyte development
            and maintenance
  JOURNAL   Development 149 (19) (2022)
   PUBMED   36178098
REFERENCE   4  (bases 1 to 2903)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2903)
  AUTHORS   Butterfield,N.C., Curry,K.F., Steinberg,J., Dewhurst,H.,
            Komla-Ebri,D., Mannan,N.S., Adoum,A.T., Leitch,V.D., Logan,J.G.,
            Waung,J.A., Ghirardello,E., Southam,L., Youlten,S.E.,
            Wilkinson,J.M., McAninch,E.A., Vancollie,V.E., Kussy,F.,
            White,J.K., Lelliott,C.J., Adams,D.J., Jacques,R., Bianco,A.C.,
            Boyde,A., Zeggini,E., Croucher,P.I., Williams,G.R. and
            Bassett,J.H.D.
  TITLE     Accelerating functional gene discovery in osteoarthritis
  JOURNAL   Nat Commun 12 (1), 467 (2021)
   PUBMED   33473114
  REMARK    Erratum:[Nat Commun. 2021 May 28;12(1):3302. doi:
            10.1038/s41467-021-23768-8.. PMID: 34050183]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2903)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 2903)
  AUTHORS   Carlone,D.L., Hart,S.R., Ladd,P.D. and Skalnik,D.G.
  TITLE     Cloning and characterization of the gene encoding the mouse
            homologue of CpG binding protein
  JOURNAL   Gene 295 (1), 71-77 (2002)
   PUBMED   12242013
REFERENCE   8  (bases 1 to 2903)
  AUTHORS   Ohki,I., Shimotake,N., Fujita,N., Jee,J., Ikegami,T., Nakao,M. and
            Shirakawa,M.
  TITLE     Solution structure of the methyl-CpG binding domain of human MBD1
            in complex with methylated DNA
  JOURNAL   Cell 105 (4), 487-497 (2001)
   PUBMED   11371345
  REMARK    Erratum:[Cell 2001 Jun 15;105(6):815]
REFERENCE   9  (bases 1 to 2903)
  AUTHORS   Hendrich,B., Abbott,C., McQueen,H., Chambers,D., Cross,S. and
            Bird,A.
  TITLE     Genomic structure and chromosomal mapping of the murine and human
            Mbd1, Mbd2, Mbd3, and Mbd4 genes
  JOURNAL   Mamm Genome 10 (9), 906-912 (1999)
   PUBMED   10441743
REFERENCE   10 (bases 1 to 2903)
  AUTHORS   Hendrich,B. and Bird,A.
  TITLE     Identification and characterization of a family of mammalian
            methyl-CpG binding proteins
  JOURNAL   Mol Cell Biol 18 (11), 6538-6547 (1998)
   PUBMED   9774669
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC147367.3.
            
            On Mar 23, 2022 this sequence version replaced NM_001357423.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF072240.1, SRR7974084.11540.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-156               AC147367.3         34003-34158         c
            157-292             AC147367.3         32898-33033         c
            293-407             AC147367.3         29113-29227         c
            408-652             AC147367.3         28779-29023         c
            653-735             AC147367.3         28619-28701         c
            736-776             AC147367.3         28380-28420         c
            777-923             AC147367.3         27875-28021         c
            924-1052            AC147367.3         27663-27791         c
            1053-1163           AC147367.3         27178-27288         c
            1164-1238           AC147367.3         27019-27093         c
            1239-1406           AC147367.3         26365-26532         c
            1407-1715           AC147367.3         25721-26029         c
            1716-1853           AC147367.3         25497-25634         c
            1854-1982           AC147367.3         25007-25135         c
            1983-2044           AC147367.3         24858-24919         c
            2045-2903           AC147367.3         19699-20557         c
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 50.7 cM"
     gene            1..2903
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /note="methyl-CpG binding domain protein 1"
                     /db_xref="GeneID:17190"
                     /db_xref="MGI:MGI:1333811"
     exon            1..156
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            157..292
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     CDS             183..2117
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /note="isoform 4 is encoded by transcript variant 4;
                     methyl-CpG-binding protein MBD1"
                     /codon_start=1
                     /product="methyl-CpG-binding domain protein 1 isoform 4"
                     /protein_id="NP_001344352.1"
                     /db_xref="CCDS:CCDS89280.1"
                     /db_xref="GeneID:17190"
                     /db_xref="MGI:MGI:1333811"
                     /translation="MAESWQDCPALGPGWKRRESFRKSGASFGRSDIYYQSPTGEKIR
                     SKVELTRYLGPACDLTLFDFRQGTLCHPIPKTHPLAVPSKKKKKPSKPAKTKKQQVGL
                     QRSEVRRETPQGEYKAPTATALASLSVSASASSSASASASSSASASASASSHAPVCCE
                     NCGIHFSWDGVKRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGDCAACLVKEDCGVCS
                     TCRLQLPSDVASGLYCKCERRRCLRIMEKSRGCGVCRGCQTQEDCGHCCICLRSPRPG
                     LKRQWRCLQRRCFWGKRDSSKRGSKVASQRHSQAPPLPPHPASQYTEPTELHISDIAP
                     TSPAEFIYYCVDEDEDELQPYTNQRQNRKCGACAACLRRMDCGRCDFCCDKPKFGGGN
                     QKRQKCRWRQCLQFAMKRLLPSAGSGSGEGAGLRPYQTHQTHQKRPASARQLQLSSPL
                     KAPWAVVTAPPGPVRDSRKQQAGRGSVLPQPDTDFVFLQEGTSSAMQMPGTAAACTEV
                     PVQAAQCSAPSWVVALPQVKQETADAPEEWTAVTTFLTSSTLQSGFPSKAADPDLSPV
                     KQEPPGPEEDGEEKKDDVSETTPAEEIGGVGTPVITEIFSLGGTRLRDAEAWLPRLHK
                     LLAVNEKEYFTELQLKEEVL"
     exon            293..407
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            408..652
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            653..735
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            736..776
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            777..923
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            924..1052
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1053..1163
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1238
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1239..1406
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..1715
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1716..1853
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1854..1982
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1983..2044
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2045..2903
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2249..2254
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /note="hexamer: AATAAT"
     regulatory      2271..2276
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /note="hexamer: AAGAAA"
     polyA_site      2281
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /note="major polyA site"
     polyA_site      2323
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /note="major polyA site"
ORIGIN      
        1 gggagtaagc aaccgccgca gaggcgactg ttttggaaga ggcactggtg gaggaggaag
       61 agaagaagag tggaacgggc cggtcgcgct agtgggatcc ttctcatggg tccacgggcc
      121 tagagcggag gaagctgcgg gcctggtgtc aagctggctt ctgttctgtc cctgaagcat
      181 ctatggctga gtcctggcag gactgcccag ccctgggccc tggctggaaa cgccgagagt
      241 cctttcgaaa gtcaggggcc tcgtttggac gctcagacat ctattaccag agccccacag
      301 gagaaaagat ccgaagcaaa gttgagctga ctcggtactt gggccctgca tgtgatctta
      361 ccctgtttga cttcagacaa ggcaccttat gccatcccat tcccaagact catcccttgg
      421 ctgtccccag caagaagaaa aagaagcctt ctaaaccagc caagactaag aaacagcagg
      481 ttgggctgca gcgaagtgag gtcaggagag agactccaca gggggaatat aaggccccta
      541 ctgccacagc tctagcttca ctatctgtgt ctgcatctgc gtcttcgtct gcatctgcat
      601 ctgcgtcttc gtctgcatct gcatctgcat ctgcgtcttc acatgcacct gtgtgctgtg
      661 agaattgtgg aatccacttt tcatgggatg gtgtcaaaag gcagagactt aagacgttgt
      721 gcaaagattg tcgagcacag agaatcgcct tcaaccgcga acagagaatg tttaagcgag
      781 tcggctgtgg ggactgtgca gcttgcctgg tgaaagagga ctgtggggtg tgctccacct
      841 gtcgcctgca actgcccagt gatgtggcct cagggcttta ctgcaagtgt gagcggagac
      901 gatgcctgcg gattatggag aagagccgag ggtgtggcgt gtgtcggggt tgtcagaccc
      961 aagaggactg tggccattgc tgcatctgtc ttcgctctcc ccgccctggt ctcaagcgcc
     1021 agtggaggtg tttgcaacgg cgctgttttt ggggtaaacg tgattctagt aagagaggct
     1081 cgaaggtagc gtctcagcgt cactcccaag ccccgccact tcctccacat cctgcatcac
     1141 agtacacaga gcccacagag ctgcacatca gcgacatagc acccacatca cctgctgagt
     1201 tcatctatta ctgtgtagac gaggacgagg acgagctaca gccctacacg aaccagcggc
     1261 agaaccgcaa gtgtggggcc tgtgcagcct gcctacggcg gatggactgt ggccgctgcg
     1321 acttctgctg cgacaagccc aaattcgggg gtggcaacca gaagcgccag aagtgtcgtt
     1381 ggcgccagtg cctgcagttt gccatgaagc ggctgctgcc cagtgctggg tcagggtctg
     1441 gggagggagc aggattgcgt ccatatcaga cccatcagac ccatcaaaag aggcctgctt
     1501 ctgctcgaca actccaactg agctctccct taaaggcccc ttgggctgtg gtcacagcac
     1561 caccaggccc tgtccgagat tcaagaaagc agcaagcagg tagaggctct gtactgcccc
     1621 agcctgacac agactttgtg tttttacaag agggtaccag cagtgccatg cagatgcctg
     1681 gcactgctgc agcttgcaca gaagtcccag tacaggcggc ccagtgctct gccccgagct
     1741 gggttgtggc cttaccccag gtgaagcaag agacggcaga tgctccagag gagtggacag
     1801 cagtcacgac cttcctgact tcctccacgc tgcagtctgg cttccctagc aaggctgcag
     1861 atccagacct ttcacctgtg aaacaagagc cccctggccc tgaagaggat ggagaagaga
     1921 aaaaagatga tgtctctgaa acaaccccag cagaggagat aggaggggtt ggcacgccag
     1981 tgatcacgga gattttcagc ctgggtggaa cccgcctccg agatgcagaa gcctggttgc
     2041 caaggctaca taaactgtta gcagtaaatg aaaaagagta ttttactgag ctgcagttga
     2101 aagaagaagt tttgtaggag agaaaagcaa ttattattaa gagaagaaag acattctttt
     2161 gtggctgaaa agctgaacct ggaattcagg aaatggaatg caaatctaga gatgtaccat
     2221 gtagagaatc tgaagtgggt gggttacaaa taattaagca cgctaattgg aagaaaacca
     2281 accttaaccg tgtatacaca tgaattgcac ttagtatctg tcagtgactg gccttattac
     2341 tgccctcccc tcccccctcc tgaccttgtt cccctacccc caggggcacc acagtagcaa
     2401 tcaattggct cttgagcagt ataactccat ttaactgtta ctgggaacta cagctctgat
     2461 ttccaagtgt gctgaagacc tgctagtttc ctggattgta ttgccagttt tgcctgggat
     2521 cctgacactc aagaatggag tatctggact tctgatccaa agccgattac tctgttcaga
     2581 ctattgtgtc cagtccagtt agacaactgc cccgccagtg tatctgatct gccctcttcc
     2641 tgtgttagcc caaactgtga gttccatttt ctgttcggtg cagtagggag attttattca
     2701 cagttgggtg actcgacaaa gcacttcggc ttcttgagcc ttaatcaact tcactgcaaa
     2761 ctaaagaaag ctaatacttt caattccttt aaccctgtac gagaattttg tcaacctcaa
     2821 aggttgatcc tgaaaattct ttgcaaattg taaaaaaaaa aaaaaaaaaa aaaacaaaca
     2881 aagaaaaaaa aaaaaaaacc aag
//
