LOCUS       NM_001357422            4487 bp    mRNA    linear   ROD 08-FEB-2025
DEFINITION  Mus musculus methyl-CpG binding domain protein 1 (Mbd1), transcript
            variant 3, mRNA.
ACCESSION   NM_001357422 XM_017317846
VERSION     NM_001357422.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Hampton,B.K., Plante,K.S., Whitmore,A.C., Linnertz,C.L.,
            Madden,E.A., Noll,K.E., Boyson,S.P., Parotti,B., Xenakis,J.G.,
            Bell,T.A., Hock,P., Shaw,G.D., de Villena,F.P., Ferris,M.T. and
            Heise,M.T.
  TITLE     Forward genetic screen of homeostatic antibody levels in the
            Collaborative Cross identifies MBD1 as a novel regulator of B cell
            homeostasis
  JOURNAL   PLoS Genet 18 (12), e1010548 (2022)
   PUBMED   36574452
  REMARK    GeneRIF: Forward genetic screen of homeostatic antibody levels in
            the Collaborative Cross identifies MBD1 as a novel regulator of B
            cell homeostasis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4487)
  AUTHORS   Zhang,H., Jiang,J.C., Wu,Y.W., Yu,Y.S., Wang,H.N., Ding,N.Z. and
            Fan,H.Y.
  TITLE     The RNA ligase RNA terminal phosphate cyclase B regulates mRNA
            alternative splicing and is required for mouse oocyte development
            and maintenance
  JOURNAL   Development 149 (19) (2022)
   PUBMED   36178098
REFERENCE   4  (bases 1 to 4487)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4487)
  AUTHORS   Butterfield,N.C., Curry,K.F., Steinberg,J., Dewhurst,H.,
            Komla-Ebri,D., Mannan,N.S., Adoum,A.T., Leitch,V.D., Logan,J.G.,
            Waung,J.A., Ghirardello,E., Southam,L., Youlten,S.E.,
            Wilkinson,J.M., McAninch,E.A., Vancollie,V.E., Kussy,F.,
            White,J.K., Lelliott,C.J., Adams,D.J., Jacques,R., Bianco,A.C.,
            Boyde,A., Zeggini,E., Croucher,P.I., Williams,G.R. and
            Bassett,J.H.D.
  TITLE     Accelerating functional gene discovery in osteoarthritis
  JOURNAL   Nat Commun 12 (1), 467 (2021)
   PUBMED   33473114
  REMARK    Erratum:[Nat Commun. 2021 May 28;12(1):3302. doi:
            10.1038/s41467-021-23768-8.. PMID: 34050183]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4487)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 4487)
  AUTHORS   Carlone,D.L., Hart,S.R., Ladd,P.D. and Skalnik,D.G.
  TITLE     Cloning and characterization of the gene encoding the mouse
            homologue of CpG binding protein
  JOURNAL   Gene 295 (1), 71-77 (2002)
   PUBMED   12242013
REFERENCE   8  (bases 1 to 4487)
  AUTHORS   Ohki,I., Shimotake,N., Fujita,N., Jee,J., Ikegami,T., Nakao,M. and
            Shirakawa,M.
  TITLE     Solution structure of the methyl-CpG binding domain of human MBD1
            in complex with methylated DNA
  JOURNAL   Cell 105 (4), 487-497 (2001)
   PUBMED   11371345
  REMARK    Erratum:[Cell 2001 Jun 15;105(6):815]
REFERENCE   9  (bases 1 to 4487)
  AUTHORS   Hendrich,B., Abbott,C., McQueen,H., Chambers,D., Cross,S. and
            Bird,A.
  TITLE     Genomic structure and chromosomal mapping of the murine and human
            Mbd1, Mbd2, Mbd3, and Mbd4 genes
  JOURNAL   Mamm Genome 10 (9), 906-912 (1999)
   PUBMED   10441743
REFERENCE   10 (bases 1 to 4487)
  AUTHORS   Hendrich,B. and Bird,A.
  TITLE     Identification and characterization of a family of mammalian
            methyl-CpG binding proteins
  JOURNAL   Mol Cell Biol 18 (11), 6538-6547 (1998)
   PUBMED   9774669
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC147367.3.
            
            On Mar 23, 2022 this sequence version replaced NM_001357422.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17784650.591587.1,
                                           SRR1660817.283350.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-156               AC147367.3         34003-34158         c
            157-292             AC147367.3         32898-33033         c
            293-537             AC147367.3         28779-29023         c
            538-620             AC147367.3         28619-28701         c
            621-661             AC147367.3         28380-28420         c
            662-808             AC147367.3         27875-28021         c
            809-937             AC147367.3         27663-27791         c
            938-1048            AC147367.3         27178-27288         c
            1049-1123           AC147367.3         27019-27093         c
            1124-1291           AC147367.3         26365-26532         c
            1292-1600           AC147367.3         25721-26029         c
            1601-1738           AC147367.3         25497-25634         c
            1739-1867           AC147367.3         25007-25135         c
            1868-1929           AC147367.3         24858-24919         c
            1930-4487           AC147367.3         21200-23757         c
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 50.7 cM"
     gene            1..4487
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /note="methyl-CpG binding domain protein 1"
                     /db_xref="GeneID:17190"
                     /db_xref="MGI:MGI:1333811"
     exon            1..156
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            157..292
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            293..537
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            538..620
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            621..661
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     CDS             653..1969
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /note="isoform 3 is encoded by transcript variant 3;
                     methyl-CpG-binding protein MBD1"
                     /codon_start=1
                     /product="methyl-CpG-binding domain protein 1 isoform 3"
                     /protein_id="NP_001344351.1"
                     /db_xref="GeneID:17190"
                     /db_xref="MGI:MGI:1333811"
                     /translation="MFKRVGCGDCAACLVKEDCGVCSTCRLQLPSDVASGLYCKCERR
                     RCLRIMEKSRGCGVCRGCQTQEDCGHCCICLRSPRPGLKRQWRCLQRRCFWGKRDSSK
                     RGSKVASQRHSQAPPLPPHPASQYTEPTELHISDIAPTSPAEFIYYCVDEDEDELQPY
                     TNQRQNRKCGACAACLRRMDCGRCDFCCDKPKFGGGNQKRQKCRWRQCLQFAMKRLLP
                     SAGSGSGEGAGLRPYQTHQTHQKRPASARQLQLSSPLKAPWAVVTAPPGPVRDSRKQQ
                     AGRGSVLPQPDTDFVFLQEGTSSAMQMPGTAAACTEVPVQAAQCSAPSWVVALPQVKQ
                     ETADAPEEWTAVTTFLTSSTLQSGFPSKAADPDLSPVKQEPPGPEEDGEEKKDDVSET
                     TPAEEIGGVGTPVITEIFSLGGTRLRDAEAWLPRSKDLKNPEAKMQ"
     exon            662..808
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            809..937
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1048
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1123
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1291
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1600
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1738
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1739..1867
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1868..1929
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1930..4487
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4469..4474
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /note="hexamer: AACAAA"
     polyA_site      4474
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
     polyA_site      4487
                     /gene="Mbd1"
                     /gene_synonym="Cxxc3; PCM1"
                     /note="major polyA site"
ORIGIN      
        1 gggagtaagc aaccgccgca gaggcgactg ttttggaaga ggcactggtg gaggaggaag
       61 agaagaagag tggaacgggc cggtcgcgct agtgggatcc ttctcatggg tccacgggcc
      121 tagagcggag gaagctgcgg gcctggtgtc aagctggctt ctgttctgtc cctgaagcat
      181 ctatggctga gtcctggcag gactgcccag ccctgggccc tggctggaaa cgccgagagt
      241 cctttcgaaa gtcaggggcc tcgtttggac gctcagacat ctattaccag agactcatcc
      301 cttggctgtc cccagcaaga agaaaaagaa gccttctaaa ccagccaaga ctaagaaaca
      361 gcaggttggg ctgcagcgaa gtgaggtcag gagagagact ccacaggggg aatataaggc
      421 ccctactgcc acagctctag cttcactatc tgtgtctgca tctgcgtctt cgtctgcatc
      481 tgcatctgcg tcttcgtctg catctgcatc tgcatctgcg tcttcacatg cacctgtgtg
      541 ctgtgagaat tgtggaatcc acttttcatg ggatggtgtc aaaaggcaga gacttaagac
      601 gttgtgcaaa gattgtcgag cacagagaat cgccttcaac cgcgaacaga gaatgtttaa
      661 gcgagtcggc tgtggggact gtgcagcttg cctggtgaaa gaggactgtg gggtgtgctc
      721 cacctgtcgc ctgcaactgc ccagtgatgt ggcctcaggg ctttactgca agtgtgagcg
      781 gagacgatgc ctgcggatta tggagaagag ccgagggtgt ggcgtgtgtc ggggttgtca
      841 gacccaagag gactgtggcc attgctgcat ctgtcttcgc tctccccgcc ctggtctcaa
      901 gcgccagtgg aggtgtttgc aacggcgctg tttttggggt aaacgtgatt ctagtaagag
      961 aggctcgaag gtagcgtctc agcgtcactc ccaagccccg ccacttcctc cacatcctgc
     1021 atcacagtac acagagccca cagagctgca catcagcgac atagcaccca catcacctgc
     1081 tgagttcatc tattactgtg tagacgagga cgaggacgag ctacagccct acacgaacca
     1141 gcggcagaac cgcaagtgtg gggcctgtgc agcctgccta cggcggatgg actgtggccg
     1201 ctgcgacttc tgctgcgaca agcccaaatt cgggggtggc aaccagaagc gccagaagtg
     1261 tcgttggcgc cagtgcctgc agtttgccat gaagcggctg ctgcccagtg ctgggtcagg
     1321 gtctggggag ggagcaggat tgcgtccata tcagacccat cagacccatc aaaagaggcc
     1381 tgcttctgct cgacaactcc aactgagctc tcccttaaag gccccttggg ctgtggtcac
     1441 agcaccacca ggccctgtcc gagattcaag aaagcagcaa gcaggtagag gctctgtact
     1501 gccccagcct gacacagact ttgtgttttt acaagagggt accagcagtg ccatgcagat
     1561 gcctggcact gctgcagctt gcacagaagt cccagtacag gcggcccagt gctctgcccc
     1621 gagctgggtt gtggccttac cccaggtgaa gcaagagacg gcagatgctc cagaggagtg
     1681 gacagcagtc acgaccttcc tgacttcctc cacgctgcag tctggcttcc ctagcaaggc
     1741 tgcagatcca gacctttcac ctgtgaaaca agagccccct ggccctgaag aggatggaga
     1801 agagaaaaaa gatgatgtct ctgaaacaac cccagcagag gagataggag gggttggcac
     1861 gccagtgatc acggagattt tcagcctggg tggaacccgc ctccgagatg cagaagcctg
     1921 gttgccaagg tccaaggacc ttaaaaatcc tgaagctaaa atgcagtaga cgggaggctt
     1981 ctgctgactg taggactgaa ggtgatattt gcagaccggc tttatgaaag acaaccctga
     2041 tctccagggg ctgggccctg gcctggttgc agagcttgag tgtgaatcaa ccctaggaga
     2101 gaaacctaca agcacaacga ggagcaggcc tgctcagtcc ttggacctta gaggaagcaa
     2161 gctggagagg aagagaaaga gagtagaagg ctggcagcac atctgctcct ccgaggatct
     2221 cagtgagatg agaagttgga tagtaggcta gggaaggata gctgaagcac tacagacaac
     2281 tcacacagcc atcttcagtg tatttgttgg tggcttctgg atgacctggc agtgtgtctg
     2341 aacattttct cagggaaaca gcagttgcaa tgctctgcaa ataaaggcgg gaaagggact
     2401 gctgtttgct gagctccgct gtggagccag cgacagtgaa cctcacagta gtctgtagga
     2461 acacactggg attgtgcact tttgcaggtg ggtaagattc agaagagatg agctttccca
     2521 gcatcaaaga gttagtgttg gtcgagtcta gaaatacacc taggctataa aagctttgtt
     2581 ctttaagcca ctaaactgtt cactgcaacc ttctgtgcaa taatgtatat tacaataaca
     2641 gtttcagtga ctgtaaacat agcagtgggt cactgtgaac actaagagat tctttatgtc
     2701 ttgcctttgt cgtggtttag gggaggccca ggctgtgagt agatggatca ccttgttagg
     2761 ccctggatgc tgcgtaggct ccctctgttc ctcagcgtgt ccttccccac cgctggctga
     2821 cagtttgctg tgcccagaca ctcgcacttc aggcagggcg gtgcttggga ctaaagctgt
     2881 tagcctgacc cctcttttcc tggcagctgc tctctgatga tctttgcttg tttcctgccc
     2941 tcagcccaaa ggtttgttag gaggctgagt gaatttatac actgagtcct acccgaggac
     3001 cagtcttggc tcagccttac ctcatttccc attcttaaaa tgccaaaaag tgtaaagttt
     3061 gttacttagt caactttaca gggcaggact gtacctcacc atatgtgtgt cctgtgtacc
     3121 ccatgtatta gctttgtccc agctctgctg tcttaggtaa tgcagatctg gatccctctc
     3181 acagacagag ctctgccacc gtggtctctg cctttgccta gttgtgtcct agaccatgct
     3241 gctcttcaac ccctagagcg cacatccact tgtcgcttgt atcctggtag ctctgacagt
     3301 gagtggtgtg ttaagggacg tgtgcgtggt tggcctttga cttgtgaatg aagattctga
     3361 ctactatgtg tgtttgacta ggggataatc aaaaggtcag aatcagtaca acaaaggctg
     3421 gtgtatgcat gttaaggacg ttttgggaga cgttttacat tatgtaattt gcagctatgg
     3481 tttagcggcc tctaggactg tcatttatat ataatgctgg gacttaatag tattcaggat
     3541 ggggagtcag gaagggtaga gtattctgga tggggagtta ggaagggtag gtggacatat
     3601 gggagccctt gaaacaagag ttggaattca agaagatggc ctggaatcca tgcagcgtct
     3661 caccgcctcc agcttcagtg atgtttatat cctgcagaag aagctagtgt ccttaggagc
     3721 agctggccta ggactggaga agctaaagga agatcccagg gcaattgtag cagctaaaag
     3781 cctggctgga ggccaggctg ttttccccat accaaggtga gacagcagac tagtgacaga
     3841 gtgtatgaac tgcaaagtgc atggtgtcct gcaccggcct cccaagtttt aacaaccgtc
     3901 cactgctgct tcccttccac tagccacagc gtttctgtgg atgttcttaa ctggaagtaa
     3961 ctggggagga tcccaggaag catagcctat agtccaggtg acatgttaga aagccactct
     4021 gccatgagcc ggctcccaag ggctcaccac tcacctgagg atgacataac aaaactacat
     4081 tgctgaaaat gtaaggccga agaccatgga tgtcatgatg ttcccagcaa gtaaagaaat
     4141 cctgtccagc tcatccgtaa caggcttgct ggtctcagcc gtgcttgttc cgttcatcca
     4201 ggtctcgaac gcttggccca tagttctcac tggacgtgac actgggattc agaaatgcag
     4261 ttttataaaa tgcatcaata tatattacaa gtgataaaaa gatctccagt ttattatgga
     4321 ctttgatgct caaatataga attgagtata ttgtggaaaa gccagtcctt ttttgagttg
     4381 gtttgttttg ttttgttgtt tttgaaagta tagaagttgg aaacaatcat ttctatttct
     4441 aaggtgttct attgtaaaat tattttaaaa caaattttct tgttcta
//
