LOCUS       NM_001285854            1844 bp    mRNA    linear   ROD 23-DEC-2022
DEFINITION  Mus musculus megakaryocyte-associated tyrosine kinase (Matk),
            transcript variant 3, mRNA.
ACCESSION   NM_001285854
VERSION     NM_001285854.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Nagy Z, Mori J, Ivanova VS, Mazharian A and Senis YA.
  TITLE     Interplay between the tyrosine kinases Chk and Csk and phosphatase
            PTPRJ is critical for regulating platelets in mice
  JOURNAL   Blood 135 (18), 1574-1587 (2020)
   PUBMED   32016283
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Kim JH, Kim K, Kim I, Seong S and Kim N.
  TITLE     c-Src-Dependent and -Independent Functions of Matk in Osteoclasts
            and Osteoblasts
  JOURNAL   J Immunol 200 (7), 2455-2463 (2018)
   PUBMED   29440352
  REMARK    GeneRIF: Matk plays a critical role in bone metabolism by impairing
            the functions of osteoclasts and osteoblasts via distinct
            mechanisms involving inhibition of c-Src-dependent and -independent
            signaling pathways.
REFERENCE   3  (bases 1 to 1844)
  AUTHORS   Lindquist S, Karitkina D, Langnaese K, Posevitz-Fejfar A, Schraven
            B, Xavier R, Seed B and Lindquist JA.
  TITLE     Phosphoprotein associated with glycosphingolipid-enriched
            microdomains differentially modulates SRC kinase activity in brain
            maturation
  JOURNAL   PLoS One 6 (9), e23978 (2011)
   PUBMED   21915273
REFERENCE   4  (bases 1 to 1844)
  AUTHORS   Lee BC, Avraham S, Imamoto A and Avraham HK.
  TITLE     Identification of the nonreceptor tyrosine kinase MATK/CHK as an
            essential regulator of immune cells using Matk/CHK-deficient mice
  JOURNAL   Blood 108 (3), 904-907 (2006)
   PUBMED   16574955
REFERENCE   5  (bases 1 to 1844)
  AUTHORS   Robinson DR, Wu YM and Lin SF.
  TITLE     The protein tyrosine kinase family of the human genome
  JOURNAL   Oncogene 19 (49), 5548-5557 (2000)
   PUBMED   11114734
  REMARK    Review article
REFERENCE   6  (bases 1 to 1844)
  AUTHORS   Ershler MA, Samokhvalov IM, Belyavsky AV and Visser JW.
  TITLE     Genomic structure and alternative splicing of the murine
            bhk/ctk/ntk gene
  JOURNAL   FEBS Lett 375 (1-2), 50-52 (1995)
   PUBMED   7498479
REFERENCE   7  (bases 1 to 1844)
  AUTHORS   Brinkley PM, Class K, Bolen JB and Penhallow RC.
  TITLE     Structure and developmental regulation of the murine ctk gene
  JOURNAL   Gene 163 (2), 179-184 (1995)
   PUBMED   7590263
REFERENCE   8  (bases 1 to 1844)
  AUTHORS   Avraham S, Jiang S, Ota S, Fu Y, Deng B, Dowler LL, White RA and
            Avraham H.
  TITLE     Structural and functional studies of the intracellular tyrosine
            kinase MATK gene and its translated product
  JOURNAL   J Biol Chem 270 (4), 1833-1842 (1995)
   PUBMED   7530249
REFERENCE   9  (bases 1 to 1844)
  AUTHORS   Ershler,M.A., Krivtsov,A.V., Krotkova,A.V., Beliavskii,A.V. and
            Visser,J.V.
  TITLE     [VNK--a new gene for nonreceptor protein-tyrosine kinase, expressed
            in the murine brain and hematopoietic system]
  JOURNAL   Dokl Akad Nauk 339 (5), 679-683 (1994)
   PUBMED   7874011
REFERENCE   10 (bases 1 to 1844)
  AUTHORS   Klages S, Adam D, Class K, Fargnoli J, Bolen JB and Penhallow RC.
  TITLE     Ctk: a protein-tyrosine kinase related to Csk that defines an
            enzyme family
  JOURNAL   Proc Natl Acad Sci U S A 91 (7), 2597-2601 (1994)
   PUBMED   7511815
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC155932.6.
            
            [WARNING] On Mar 15, 2024 this sequence was replaced by
            NM_001285854.2.
            
            Transcript Variant: This variant (3) contains a distinct 5' UTR and
            lacks an in-frame portion of the 5' coding region, compared to
            variant 1. The resulting isoform (3) has a shorter N-terminus,
            compared to isoform 1. Both variants 3 and 4 encode the same
            isoform (3).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U05210.1, AK013606.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-276               AC155932.6         216905-217180
            277-336             AC155932.6         218086-218145
            337-447             AC155932.6         218783-218893
            448-563             AC155932.6         218989-219104
            564-783             AC155932.6         219193-219412
            784-877             AC155932.6         219695-219788
            878-943             AC155932.6         220514-220579
            944-1043            AC155932.6         221049-221148
            1044-1128           AC155932.6         221237-221321
            1129-1202           AC155932.6         221401-221474
            1203-1398           AC155932.6         221567-221762
            1399-1485           AC155932.6         222054-222140
            1486-1844           AC155932.6         222229-222587
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 39.72 cM"
     gene            1..1844
                     /gene="Matk"
                     /gene_synonym="CHK; HYL; Ntk; p56ntk"
                     /note="megakaryocyte-associated tyrosine kinase"
                     /db_xref="GeneID:17179"
                     /db_xref="MGI:MGI:99259"
     exon            1..276
                     /gene="Matk"
                     /gene_synonym="CHK; HYL; Ntk; p56ntk"
                     /inference="alignment:Splign:2.1.0"
     exon            277..336
                     /gene="Matk"
                     /gene_synonym="CHK; HYL; Ntk; p56ntk"
                     /inference="alignment:Splign:2.1.0"
     CDS             328..1725
                     /gene="Matk"
                     /gene_synonym="CHK; HYL; Ntk; p56ntk"
                     /EC_number="2.7.10.2"
                     /note="isoform 3 is encoded by transcript variant 3;
                     megakaryocyte-associated tyrosine-protein kinase; protein
                     kinase NTK; tyrosine-protein kinase CTK; Csk homologous
                     kinase; tyrosine protein kinase"
                     /codon_start=1
                     /product="megakaryocyte-associated tyrosine-protein kinase
                     isoform 3"
                     /protein_id="NP_001272783.1"
                     /db_xref="CCDS:CCDS70078.1"
                     /db_xref="GeneID:17179"
                     /db_xref="MGI:MGI:99259"
                     /translation="MPTRWAPGTQCMTKCENSRPKPGELAFRKGDMVTILEACEDKSW
                     YRAKHHGSGQEGLLAAAALRQREALSTDPKLSLMPWFHGKISGQEAIQQLQPPEDGLF
                     LVRESARHPGDYVLCVSFGRDVIHYRVLHRDGHLTIDEAVCFCNLMDMVEHYTKDKGA
                     ICTKLVKPRRKQGAKSAEEELAKAGWLLDLQHLTLGAQIGEGEFGAVLQGEYLGQKVA
                     VKNIKCDVTAQAFLDETAVMTKLQHRNLVRLLGVILHHGLYIVMEHVSKGNLVNFLRT
                     RGRALVSTSQLLQFALHVAEGMEYLESKKLVHRDLAARNILVSEDLVAKVSDFGLAKA
                     ERKGLDSSRLPVKWTAPEALKNGRFSSKSDVWSFGVLLWEVFSYGRAPYPKMSLKEVS
                     EAVEKGYRMEPPDGCPGSVHTLMGSCWEAEPARRPPFRKIVEKLGRELRSVGVSAPAG
                     GQEAEGSAPTRSQDP"
     exon            337..447
                     /gene="Matk"
                     /gene_synonym="CHK; HYL; Ntk; p56ntk"
                     /inference="alignment:Splign:2.1.0"
     exon            448..563
                     /gene="Matk"
                     /gene_synonym="CHK; HYL; Ntk; p56ntk"
                     /inference="alignment:Splign:2.1.0"
     exon            564..783
                     /gene="Matk"
                     /gene_synonym="CHK; HYL; Ntk; p56ntk"
                     /inference="alignment:Splign:2.1.0"
     exon            784..877
                     /gene="Matk"
                     /gene_synonym="CHK; HYL; Ntk; p56ntk"
                     /inference="alignment:Splign:2.1.0"
     exon            878..943
                     /gene="Matk"
                     /gene_synonym="CHK; HYL; Ntk; p56ntk"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1043
                     /gene="Matk"
                     /gene_synonym="CHK; HYL; Ntk; p56ntk"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1128
                     /gene="Matk"
                     /gene_synonym="CHK; HYL; Ntk; p56ntk"
                     /inference="alignment:Splign:2.1.0"
     exon            1129..1202
                     /gene="Matk"
                     /gene_synonym="CHK; HYL; Ntk; p56ntk"
                     /inference="alignment:Splign:2.1.0"
     exon            1203..1398
                     /gene="Matk"
                     /gene_synonym="CHK; HYL; Ntk; p56ntk"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..1485
                     /gene="Matk"
                     /gene_synonym="CHK; HYL; Ntk; p56ntk"
                     /inference="alignment:Splign:2.1.0"
     exon            1486..1844
                     /gene="Matk"
                     /gene_synonym="CHK; HYL; Ntk; p56ntk"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tttcccaggg tgggcgaagg aaggctagga ttcagcgggg acccgagagc acccggcact
       61 tggtactcag cactctgccc ggacggagtg ggaggcgcag cgggaggagg tggcagcgcg
      121 gtcttagtga gaccggcgct aggacccgcg ggggctccga gccgggtccc ctccatcctt
      181 gccccaaaat gaggaagcct tgcaagttgc tccgagttga gccactgggt gctcgacccg
      241 ctggcaggga ccctccgagg gtcgcgccct aagcaggtga gccctagatt gttcggagct
      301 tggcaccccg cgcctgctgc agctaggatg ccaacgcgct gggcccctgg gactcaatgc
      361 atgaccaagt gtgagaactc tcgccccaag cccggtgagc tagcctttcg aaagggtgac
      421 atggtgacca tcttggaggc ctgtgaggac aagagctggt accgagccaa gcaccatggc
      481 agtgggcagg aagggctgct ggcggccgct gctctgcgac agcgggaggc cctctccaca
      541 gaccccaagc tcagcctcat gccatggttt catggcaaga tctccggcca ggaagccata
      601 cagcagctgc agccacccga ggacgggctg ttccttgtga gggaatcagc tcgtcaccct
      661 ggagactatg tcttgtgtgt cagtttcggc cgtgacgtca tccactaccg tgttttgcat
      721 cgagatgggc acctcaccat cgatgaggcc gtgtgtttct gtaacctgat ggacatggtg
      781 gagcactaca ccaaggacaa gggggccatc tgcaccaagc tggtgaagcc aaggaggaaa
      841 cagggcgcaa agtctgcaga ggaggagctc gccaaggctg gctggctact cgacctgcag
      901 catctgactc tgggagcaca gattggagag ggggagtttg gagccgtcct acagggtgag
      961 tacctgggac agaaggtggc tgtgaagaat atcaagtgtg atgtgacagc ccaggccttc
     1021 ctggatgaga cggctgtgat gacgaagctg cagcacagga acctagtgcg actcctgggt
     1081 gtgatcctgc accacggctt gtacattgtc atggagcacg tgagcaaggg caacctggtg
     1141 aacttcctgc gcacgcgggg ccgtgctctt gtgagcacct ctcagcttct gcagtttgct
     1201 cttcatgttg ctgaaggcat ggaatacctg gagagcaaga agctggtgca ccgggacctg
     1261 gctgctcgga acatcctggt ctctgaggac ttggtggcca aggtcagtga ctttggctta
     1321 gccaaggcag agcgcaaggg gctggactca agccggctgc cagtcaagtg gacggcacct
     1381 gaggctctca aaaacgggcg gttctccagc aagtcggatg tctggagttt tggggtgctg
     1441 ttgtgggaag tcttctctta tggaagagcc ccatacccca agatgtcgct aaaggaggtt
     1501 tcagaggctg tggagaaggg ttaccgcatg gagccccccg atggctgccc aggctctgtg
     1561 cacaccctca tgggtagctg ctgggaggca gagcctgcgc gccgaccacc cttccgcaaa
     1621 atagtggaga agctgggccg tgagctccgc agtgtgggtg tctcggcccc cgctggggga
     1681 caggaggctg agggctcagc tcccacacgg agccaggacc cctgatccct gaagcagaaa
     1741 gcaagaggac atagatgcag ggtcacagag ccaggaagag ctctgcagcc cctgagaccc
     1801 ccatgcctgg gacactccat aaagagcatt tggaaggatt ttgt
//
