LOCUS       NM_145569               2805 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus methionine adenosyltransferase 2A (Mat2a), transcript
            variant 1, mRNA.
ACCESSION   NM_145569
VERSION     NM_145569.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Li,M.L., Cao,S.Y., Qu,J., Zhang,L., Gao,Q., Wang,X., Yin,M.,
            Liu,Y., Lei,M.Z. and Lei,Q.Y.
  TITLE     S-adenosyl-L-methionine supplementation alleviates damaged
            intestinal epithelium and inflammatory infiltration caused by Mat2a
            deficiency
  JOURNAL   Development 150 (20) (2023)
   PUBMED   36975381
REFERENCE   3  (bases 1 to 2805)
  AUTHORS   Li,J.T., Yang,H., Lei,M.Z., Zhu,W.P., Su,Y., Li,K.Y., Zhu,W.Y.,
            Wang,J., Zhang,L., Qu,J., Lv,L., Lu,H.J., Chen,Z.J., Wang,L.,
            Yin,M. and Lei,Q.Y.
  TITLE     Dietary folate drives methionine metabolism to promote cancer
            development by stabilizing MAT IIA
  JOURNAL   Signal Transduct Target Ther 7 (1), 192 (2022)
   PUBMED   35729157
  REMARK    GeneRIF: Dietary folate drives methionine metabolism to promote
            cancer development by stabilizing MAT IIA.
            Erratum:[Signal Transduct Target Ther. 2022 Dec 28;7(1):401. doi:
            10.1038/s41392-022-01255-w.. PMID: 36577756]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2805)
  AUTHORS   Golbourn,B.J., Halbert,M.E., Halligan,K., Varadharajan,S., Krug,B.,
            Mbah,N.E., Kabir,N., Stanton,A.J., Locke,A.L., Casillo,S.M.,
            Zhao,Y., Sanders,L.M., Cheney,A., Mullett,S.J., Chen,A.,
            Wassell,M., Andren,A., Perez,J., Jane,E.P., Premkumar,D.R.D.,
            Koncar,R.F., Mirhadi,S., McCarl,L.H., Chang,Y.F., Wu,Y.L.,
            Gatesman,T.A., Cruz,A.F., Zapotocky,M., Hu,B., Kohanbash,G.,
            Wang,X., Vartanian,A., Moran,M.F., Lieberman,F., Amankulor,N.M.,
            Wendell,S.G., Vaske,O.M., Panigrahy,A., Felker,J., Bertrand,K.C.,
            Kleinman,C.L., Rich,J.N., Friedlander,R.M., Broniscer,A.,
            Lyssiotis,C., Jabado,N., Pollack,I.F., Mack,S.C. and Agnihotri,S.
  TITLE     Loss of MAT2A compromises methionine metabolism and represents a
            vulnerability in H3K27M mutant glioma by modulating the epigenome
  JOURNAL   Nat Cancer 3 (5), 629-648 (2022)
   PUBMED   35422502
  REMARK    GeneRIF: Loss of MAT2A compromises methionine metabolism and
            represents a vulnerability in H3K27M mutant glioma by modulating
            the epigenome.
            Erratum:[Nat Cancer. 2022 Jul;3(7):899. doi:
            10.1038/s43018-022-00407-9.. PMID: 35739422]
REFERENCE   5  (bases 1 to 2805)
  AUTHORS   Kang,H., Guo,Q., Dong,Y., Peng,R., Song,K., Wang,J., Liu,H.,
            Zhu,M., Zhao,H., Guan,H. and Li,F.
  TITLE     Inhibition of MAT2A suppresses osteoclastogenesis and prevents
            ovariectomy-induced bone loss
  JOURNAL   FASEB J 36 (2), e22167 (2022)
   PUBMED   35064691
  REMARK    GeneRIF: Inhibition of MAT2A suppresses osteoclastogenesis and
            prevents ovariectomy-induced bone loss.
            Erratum:[FASEB J. 2023 Mar;37(3):e22755. doi: 10.1096/fsb2.22755..
            PMID: 36786683]
REFERENCE   6  (bases 1 to 2805)
  AUTHORS   Ramani,K., Yang,H., Kuhlenkamp,J., Tomasi,L., Tsukamoto,H.,
            Mato,J.M. and Lu,S.C.
  TITLE     Changes in the expression of methionine adenosyltransferase genes
            and S-adenosylmethionine homeostasis during hepatic stellate cell
            activation
  JOURNAL   Hepatology 51 (3), 986-995 (2010)
   PUBMED   20043323
  REMARK    GeneRIF: MAT2A and MAT2beta genes are induced during hepatic
            stellate cell activation
REFERENCE   7  (bases 1 to 2805)
  AUTHORS   Li,S., Arning,E., Liu,C., Vitvitsky,V., Hernandez,C., Banerjee,R.,
            Bottiglieri,T. and Lin,J.D.
  TITLE     Regulation of homocysteine homeostasis through the transcriptional
            coactivator PGC-1alpha
  JOURNAL   Am J Physiol Endocrinol Metab 296 (3), E543-E548 (2009)
   PUBMED   19158324
REFERENCE   8  (bases 1 to 2805)
  AUTHORS   Chen,H., Xia,M., Lin,M., Yang,H., Kuhlenkamp,J., Li,T., Sodir,N.M.,
            Chen,Y.H., Josef-Lenz,H., Laird,P.W., Clarke,S., Mato,J.M. and
            Lu,S.C.
  TITLE     Role of methionine adenosyltransferase 2A and S-adenosylmethionine
            in mitogen-induced growth of human colon cancer cells
  JOURNAL   Gastroenterology 133 (1), 207-218 (2007)
   PUBMED   17631143
  REMARK    GeneRIF: up-regulation of MAT2A also provides a growth advantage
            and s-adenosylmethionine and methylthioadenosine can block
            mitogenic signaling in colon cancer cells.
            Erratum:[Gastroenterology. 2007 Nov;133(5):1747]
REFERENCE   9  (bases 1 to 2805)
  AUTHORS   Farrar,C. and Clarke,S.
  TITLE     Altered levels of S-adenosylmethionine and S-adenosylhomocysteine
            in the brains of L-isoaspartyl (D-Aspartyl)
            O-methyltransferase-deficient mice
  JOURNAL   J Biol Chem 277 (31), 27856-27863 (2002)
   PUBMED   12023972
REFERENCE   10 (bases 1 to 2805)
  AUTHORS   LeGros,H.L. Jr., Halim,A.B., Geller,A.M. and Kotb,M.
  TITLE     Cloning, expression, and functional characterization of the beta
            regulatory subunit of human methionine adenosyltransferase (MAT II)
  JOURNAL   J Biol Chem 275 (4), 2359-2366 (2000)
   PUBMED   10644686
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC116115.11.
            
            On Jun 3, 2018 this sequence version replaced NM_145569.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK166671.1, AK052764.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-211               AC116115.11        203434-203644       c
            212-289             AC116115.11        201459-201536       c
            290-412             AC116115.11        201083-201205       c
            413-525             AC116115.11        200505-200617       c
            526-669             AC116115.11        200285-200428       c
            670-888             AC116115.11        199899-200117       c
            889-1071            AC116115.11        199294-199476       c
            1072-1205           AC116115.11        199069-199202       c
            1206-2805           AC116115.11        196884-198483       c
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 32.27 cM"
     gene            1..2805
                     /gene="Mat2a"
                     /gene_synonym="D630045P18Rik; MAT 2"
                     /note="methionine adenosyltransferase 2A"
                     /db_xref="GeneID:232087"
                     /db_xref="MGI:MGI:2443731"
     exon            1..211
                     /gene="Mat2a"
                     /gene_synonym="D630045P18Rik; MAT 2"
                     /inference="alignment:Splign:2.1.0"
     CDS             121..1308
                     /gene="Mat2a"
                     /gene_synonym="D630045P18Rik; MAT 2"
                     /EC_number="2.5.1.6"
                     /note="isoform 1 is encoded by transcript variant 1;
                     adoMet synthetase 2; methionine adenosyltransferase II,
                     alpha; methionine adenosyltransferase 2; adoMet synthase
                     2"
                     /codon_start=1
                     /product="S-adenosylmethionine synthase isoform type-2
                     isoform 1"
                     /protein_id="NP_663544.1"
                     /db_xref="CCDS:CCDS39515.1"
                     /db_xref="GeneID:232087"
                     /db_xref="MGI:MGI:2443731"
                     /translation="MNGQLNGFHEAFIEEGTFLFTSESVGEGHPDKICDQISDAVLDA
                     HLQQDPDAKVACETVAKTGMILLAGEITSRAAIDYQKVVREAIKHIGYDDSSKGFDYK
                     TCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKL
                     NAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEM
                     RDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGA
                     HGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFH
                     YGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKKPIYQRTAAYGHFGRDSFPWEVPKK
                     LKY"
     exon            212..289
                     /gene="Mat2a"
                     /gene_synonym="D630045P18Rik; MAT 2"
                     /inference="alignment:Splign:2.1.0"
     exon            290..412
                     /gene="Mat2a"
                     /gene_synonym="D630045P18Rik; MAT 2"
                     /inference="alignment:Splign:2.1.0"
     exon            413..525
                     /gene="Mat2a"
                     /gene_synonym="D630045P18Rik; MAT 2"
                     /inference="alignment:Splign:2.1.0"
     exon            526..669
                     /gene="Mat2a"
                     /gene_synonym="D630045P18Rik; MAT 2"
                     /inference="alignment:Splign:2.1.0"
     exon            670..888
                     /gene="Mat2a"
                     /gene_synonym="D630045P18Rik; MAT 2"
                     /inference="alignment:Splign:2.1.0"
     exon            889..1071
                     /gene="Mat2a"
                     /gene_synonym="D630045P18Rik; MAT 2"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1205
                     /gene="Mat2a"
                     /gene_synonym="D630045P18Rik; MAT 2"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..2805
                     /gene="Mat2a"
                     /gene_synonym="D630045P18Rik; MAT 2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2781..2786
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mat2a"
                     /gene_synonym="D630045P18Rik; MAT 2"
                     /note="hexamer: AATAAA"
     polyA_site      2805
                     /gene="Mat2a"
                     /gene_synonym="D630045P18Rik; MAT 2"
                     /note="major polyA site"
ORIGIN      
        1 gttcgctccg agtctccagc ctgccgagag ctgaagcgat cctccctctg tgtcttccgc
       61 agccctccgc ctcgccgcct ctcagtctcc tcagagtcgc cctctctcac cgccgccgcc
      121 atgaacgggc agctcaacgg cttccacgag gcgttcattg aggaggggac gttcctcttc
      181 acttccgagt ctgtagggga aggtcaccca gataagattt gtgaccaaat cagtgatgct
      241 gtccttgatg cacaccttca acaagaccct gatgctaaag tggcttgtga aactgttgct
      301 aaaactggaa tgattcttct tgctggggaa attacatcca gagctgccat tgattaccag
      361 aaagtggttc gtgaagccat aaagcacatt ggatatgatg actcttccaa agggtttgac
      421 tacaagactt gtaatgtgtt ggttgccttg gaacaacagt caccagatat tgcccaaggt
      481 gttcatcttg accggaatga ggaagatatt ggtgcaggag accagggttt gatgtttggt
      541 tatgccactg atgaaactga agagtgtatg cctttaacca ttgtcttagc acacaagcta
      601 aatgccaaat tggctgaact acgccgcaat ggtacattgc cttggttacg cccagattct
      661 aaaactcaag tgactgtgca atatatgcaa gatcggggtg ctgtgcttcc catcagagtc
      721 cacacgattg ttatatctgt tcagcacgat gaagaagttt gtcttgatga gatgagggat
      781 gctctgaagg agaaagtgat caaggctgtt gtacctgcaa aataccttga tgaggataca
      841 atttaccacc tacagccaag tggcagattt gttattggtg ggcctcaggg tgatgctggc
      901 ctgactggcc gaaaaatcat tgtggatact tatggcggtt ggggagctca tggaggaggg
      961 gccttttcag gaaaggatta taccaaagtg gaccgttcag ctgcttatgc tgctcgttgg
     1021 gtggcaaaat cccttgttaa aggaggtctg tgcaggaggg ttcttgttca ggtctcttat
     1081 gctattggag tttctcatcc attgtcgatc tccattttcc attatggcac ttctcagaag
     1141 agtgagagag agctattaga aattgtaaag aagaattttg atcttcgccc tggggtcatt
     1201 gtcagggatc tggatctgaa gaagccaatt tatcagagga ctgcagccta tggccacttt
     1261 ggtagggaca gcttcccatg ggaagtgccc aaaaagctta aatattgaaa gtgttagcct
     1321 tttttcccca gacttgttgg cgtaggttac agagaagcct tcaagctctg agggaaaggg
     1381 ccttcttcct aaatttttct gtcctctttc agctcctgat cagttgcagt cactctaatc
     1441 aatgacatga attttagctt ttgtggggga ctgtaagttg ggcttgctat tctgtcccta
     1501 ggtgttttgt tcaccattat aatggatata gtgagcatag gtgagccatg taactgccta
     1561 gaaacaaaca ctgtagtgaa taatgctttg aaatcgaacc tttgtgccct atcacctaat
     1621 cctccaaagt cctaattgca attactttcc caccagatgc tgaaaacatc cttgtagtgt
     1681 gcacgtaaag tacttgtagt ttgactgtag actctggcaa tgccacagcc ctgtcagcat
     1741 gaatctgtaa cgtcttgagc tctatttatt gaatgtgaag cccctgtcct tatcctccct
     1801 gtaactcatt tctaattatg tagttcttta tcagggagtg ttcctatcca atcaatcttg
     1861 catgaaacga aagtttcagt tggagctcta gcctgacttt gaaaaaaaaa aaaaaaaagg
     1921 cagttacgat taaaccatct ccctggtgct tatgctatta attgccacct caaacagcac
     1981 caaatcaaaa tctctccact tttcagctgt ctttggagga cgtacgtaat aaggttttat
     2041 ttagtaaacc aaccctatgc atggtttcag cactagccaa acctcaccaa cttttagtct
     2101 agaaaaacag gcatttggca cccttgtgat atacagagaa gtcacagggc agtacctgag
     2161 ggtctgtagg ttgcacactt tggtaccagg taactttttt ttctttataa gaaagagtac
     2221 tccacactgc acaatagctc ctcccagggt tttaactttg ttttattttc aaaaccaggt
     2281 ccaatgagct ttctgaacag ctggtgtagc tacagagaaa ccagcttcct tcagagagca
     2341 gtgcttttgg cggggaggag gaaatccctt catacttgaa cattttctaa ttgcttattt
     2401 attgtattct ggggtatggc gtaagtacag agaagccatc acctcagatg gcagctttta
     2461 aaagattttt ttttttcttc aacaccatga attcctttaa catgtttcca gcattcccag
     2521 gtaggccaag gtgtcctaca gaaaaacctt gggttagacc tacagggggt ctggctggtg
     2581 ttaacagaag ggagggcaga gctggtgcag ctggccatgg agaaagctga cttggctggt
     2641 gtggtacaga gaagccagct tgtttacatg cttattccat gactgcttgc cctaagcaag
     2701 aaagtgcctt tcaggatcta ttttttggag gtttattacg tatgtctggt tctcaattcc
     2761 aacagtttaa tgaagatcta aataaaatgc taggttctac ccaaa
//
