LOCUS       NM_025979               4652 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus microtubule associated serine/threonine kinase-like
            (Mastl), mRNA.
ACCESSION   NM_025979
VERSION     NM_025979.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Sanz-Castillo,B., Hurtado,B., Vara-Ciruelos,D., El Bakkali,A.,
            Hermida,D., Salvador-Barbero,B., Martinez-Alonso,D.,
            Gonzalez-Martinez,J., Santiveri,C., Campos-Olivas,R.,
            Ximenez-Embun,P., Munoz,J., Alvarez-Fernandez,M. and Malumbres,M.
  TITLE     The MASTL/PP2A cell cycle kinase-phosphatase module restrains
            PI3K-Akt activity in an mTORC1-dependent manner
  JOURNAL   EMBO J 42 (2), e110833 (2023)
   PUBMED   36354735
  REMARK    GeneRIF: The MASTL/PP2A cell cycle kinase-phosphatase module
            restrains PI3K-Akt activity in an mTORC1-dependent manner.
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Bisteau,X., Lee,J., Srinivas,V., Lee,J.H.S., Niska-Blakie,J.,
            Tan,G., Yap,S.Y.X., Hom,K.W., Wong,C.K., Chae,J., Wang,L.C.,
            Kim,J., Rancati,G., Sobota,R.M., Tan,C.S.H. and Kaldis,P.
  TITLE     The Greatwall kinase safeguards the genome integrity by affecting
            the kinome activity in mitosis
  JOURNAL   Oncogene 39 (44), 6816-6840 (2020)
   PUBMED   32978522
  REMARK    GeneRIF: The Greatwall kinase safeguards the genome integrity by
            affecting the kinome activity in mitosis.
REFERENCE   3  (bases 1 to 4652)
  AUTHORS   Hurtado,B., Trakala,M., Ximenez-Embun,P., El Bakkali,A.,
            Partida,D., Sanz-Castillo,B., Alvarez-Fernandez,M., Maroto,M.,
            Sanchez-Martinez,R., Martinez,L., Munoz,J., Garcia de Frutos,P. and
            Malumbres,M.
  TITLE     Thrombocytopenia-associated mutations in Ser/Thr kinase MASTL
            deregulate actin cytoskeletal dynamics in platelets
  JOURNAL   J Clin Invest 128 (12), 5351-5367 (2018)
   PUBMED   30252678
  REMARK    GeneRIF: reveal an unexpected role of Mastl in actin cytoskeletal
            dynamics in postmitotic cells and suggest that the
            thrombocytopenia-associated mutation in MASTL is a pathogenic
            dominant mutation that mimics decreased PP2A activity resulting in
            altered phosphorylation of cytoskeletal regulatory pathways.
REFERENCE   4  (bases 1 to 4652)
  AUTHORS   Soeda,S., Yamada-Nomoto,K., Michiue,T. and Ohsugi,M.
  TITLE     RSK-MASTL Pathway Delays Meiotic Exit in Mouse Zygotes to Ensure
            Paternal Chromosome Stability
  JOURNAL   Dev Cell 47 (3), 363-376 (2018)
   PUBMED   30293837
  REMARK    GeneRIF: Findings reveal the role of RSK in mouse oocytes, showing
            that the RSK-MASTL pathway allows mammalian-specific prolonged
            meiotic exit and ensures the faithful conversion from sperm to
            paternal pronuclei.
REFERENCE   5  (bases 1 to 4652)
  AUTHORS   Risal,S., Zhang,J., Adhikari,D., Liu,X., Shao,J., Hu,M.,
            Busayavalasa,K., Tu,Z., Chen,Z., Kaldis,P. and Liu,K.
  TITLE     MASTL is essential for anaphase entry of proliferating primordial
            germ cells and establishment of female germ cells in mice
  JOURNAL   Cell Discov 3, 16052 (2017)
   PUBMED   28224044
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4652)
  AUTHORS   Diril,M.K., Bisteau,X., Kitagawa,M., Caldez,M.J., Wee,S.,
            Gunaratne,J., Lee,S.H. and Kaldis,P.
  TITLE     Loss of the Greatwall Kinase Weakens the Spindle Assembly
            Checkpoint
  JOURNAL   PLoS Genet 12 (9), e1006310 (2016)
   PUBMED   27631493
  REMARK    GeneRIF: using in vitro dephosphorylation assays, we demonstrate
            that Mastl promotes persistent MPS1 phosphorylation by inhibiting
            PP2A/B55-mediated MPS1 dephosphorylation rather than affecting Cdk1
            kinase activity. Our findings establish a key regulatory function
            of the Greatwall kinase/Mastl - PP2A/B55 pathway in preventing
            premature SAC silencing
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 4652)
  AUTHORS   Adhikari,D., Diril,M.K., Busayavalasa,K., Risal,S., Nakagawa,S.,
            Lindkvist,R., Shen,Y., Coppola,V., Tessarollo,L., Kudo,N.R.,
            Kaldis,P. and Liu,K.
  TITLE     Mastl is required for timely activation of APC/C in meiosis I and
            Cdk1 reactivation in meiosis II
  JOURNAL   J Cell Biol 206 (7), 843-853 (2014)
   PUBMED   25246615
  REMARK    GeneRIF: Mastl is required for the timely activation of
            anaphase-promoting complex/cyclosome to allow meiosis I exit and
            for the rapid rise of Cdk1 activity.
REFERENCE   8  (bases 1 to 4652)
  AUTHORS   Alvarez-Fernandez,M., Sanchez-Martinez,R., Sanz-Castillo,B.,
            Gan,P.P., Sanz-Flores,M., Trakala,M., Ruiz-Torres,M., Lorca,T.,
            Castro,A. and Malumbres,M.
  TITLE     Greatwall is essential to prevent mitotic collapse after nuclear
            envelope breakdown in mammals
  JOURNAL   Proc Natl Acad Sci U S A 110 (43), 17374-17379 (2013)
   PUBMED   24101512
  REMARK    GeneRIF: Data show that Mastl (Greatwall)-null cells display
            mitotic collapse after nuclear envelope breakdown (NEB)
            characterized by defective chromosome condensation and prometaphase
            arrest.
REFERENCE   9  (bases 1 to 4652)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 4652)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL845257.2, AK030140.1, CN536399.1 and CD546688.1.
            
            On Jul 21, 2009 this sequence version replaced NM_025979.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK030140.1, BC103779.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164143 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-108               AL845257.2         139306-139413       c
            109-367             AK030140.1         4-262
            368-935             CN536399.1         141-708
            936-1320            CD546688.1         159-543
            1321-3131           AK030140.1         1216-3026
            3132-4652           AL845257.2         99933-101453        c
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 15.23 cM"
     gene            1..4652
                     /gene="Mastl"
                     /gene_synonym="2700091H24Rik; GW; GWL; MAST-L; THC2"
                     /note="microtubule associated serine/threonine
                     kinase-like"
                     /db_xref="GeneID:67121"
                     /db_xref="MGI:MGI:1914371"
     exon            1..346
                     /gene="Mastl"
                     /gene_synonym="2700091H24Rik; GW; GWL; MAST-L; THC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             164..2761
                     /gene="Mastl"
                     /gene_synonym="2700091H24Rik; GW; GWL; MAST-L; THC2"
                     /EC_number="2.7.11.1"
                     /note="microtubule-associated serine/threonine-protein
                     kinase-like"
                     /codon_start=1
                     /product="serine/threonine-protein kinase greatwall"
                     /protein_id="NP_080255.3"
                     /db_xref="CCDS:CCDS15727.1"
                     /db_xref="GeneID:67121"
                     /db_xref="MGI:MGI:1914371"
                     /translation="MESASASEENEGGAAIEECVSRIPVPRPPSIEEFTIVKPISRGA
                     FGKVYLGQKGGKLYAVKVVKKADMINKNMTHQVQAERDALALSKSPFVVHLYYSLQSA
                     SNIYLIMEYLIGGDVKSLLHIYGYFDEEMAIKYISEVALALDYLHRHGIIHRDLKPDN
                     MLISNEGHIKLTDFGLSKVTLNRDINMMDILTTPSMSKPKQDYSRTPGQVLSLISSLG
                     FFTPVGEKDQDSANMFSAPKSAAQLSRGFICPMSVDQKEPTSYSSKLLKSCFETLSSN
                     PEIPVKCLTSNLLQCRKRLGTSSTSSQSHTFVSSVESECHSNPKWERDCQSTESSGCA
                     MSWNAVEMLYAKSTSAIKTKTELELALSPIHDSSAIPAAGSNQVTLPRKCFREISWEA
                     RDPDNENMTIDKGQSGFCQSSQRSVNSSATSEEHLGKRNYKRNFHLVDSSPCQEIMQS
                     KKNCTEYEANKERQGCRANQSTGLTTEVQNLKLSGCESQQLDYANKENIVTYLTDRQT
                     PEKLHIPTIAKNLMSELDEDCELSSKKDCLSSNSVCSDEDRALKTTCVDSDSSFPGVS
                     MMESSLEIQALEPDKSIRDYSFEEPNTEDLFVLPKCQENSLPQDDCHACIQDSSQVSA
                     HPSKAPKALTSKINVVAFRSFNSHINASTNSEPSKISITSLDAMDISCDYSGSYPMAV
                     SPTEKGRHYTSHQTPNQVKLGTSYRTPKSVRRGAAPVDDGRILGTPDYLAPELLLGTA
                     HGPAVDWWALGVCLFEFLTGIPPFNDETPQQVFQNILKRDIPWPEGEEKLSDNAQSAM
                     DMLLTIDDSKRAGMRELKQHPLFSEVDWENLQHQTMPFVPQPDDETDTSYFEARNNAQ
                     HLTISGFSL"
     exon            347..484
                     /gene="Mastl"
                     /gene_synonym="2700091H24Rik; GW; GWL; MAST-L; THC2"
                     /inference="alignment:Splign:2.1.0"
     exon            485..624
                     /gene="Mastl"
                     /gene_synonym="2700091H24Rik; GW; GWL; MAST-L; THC2"
                     /inference="alignment:Splign:2.1.0"
     exon            625..713
                     /gene="Mastl"
                     /gene_synonym="2700091H24Rik; GW; GWL; MAST-L; THC2"
                     /inference="alignment:Splign:2.1.0"
     exon            714..820
                     /gene="Mastl"
                     /gene_synonym="2700091H24Rik; GW; GWL; MAST-L; THC2"
                     /inference="alignment:Splign:2.1.0"
     exon            821..968
                     /gene="Mastl"
                     /gene_synonym="2700091H24Rik; GW; GWL; MAST-L; THC2"
                     /inference="alignment:Splign:2.1.0"
     exon            969..1141
                     /gene="Mastl"
                     /gene_synonym="2700091H24Rik; GW; GWL; MAST-L; THC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..2248
                     /gene="Mastl"
                     /gene_synonym="2700091H24Rik; GW; GWL; MAST-L; THC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2249..2387
                     /gene="Mastl"
                     /gene_synonym="2700091H24Rik; GW; GWL; MAST-L; THC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2388..2501
                     /gene="Mastl"
                     /gene_synonym="2700091H24Rik; GW; GWL; MAST-L; THC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2502..2603
                     /gene="Mastl"
                     /gene_synonym="2700091H24Rik; GW; GWL; MAST-L; THC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2604..4652
                     /gene="Mastl"
                     /gene_synonym="2700091H24Rik; GW; GWL; MAST-L; THC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3036..3041
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mastl"
                     /gene_synonym="2700091H24Rik; GW; GWL; MAST-L; THC2"
                     /note="hexamer: ACTAAA"
     polyA_site      3057
                     /gene="Mastl"
                     /gene_synonym="2700091H24Rik; GW; GWL; MAST-L; THC2"
     regulatory      4552..4557
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mastl"
                     /gene_synonym="2700091H24Rik; GW; GWL; MAST-L; THC2"
                     /note="hexamer: CATAAA"
     polyA_site      4572
                     /gene="Mastl"
                     /gene_synonym="2700091H24Rik; GW; GWL; MAST-L; THC2"
                     /note="major polyA site"
     polyA_site      4652
                     /gene="Mastl"
                     /gene_synonym="2700091H24Rik; GW; GWL; MAST-L; THC2"
                     /note="major polyA site"
ORIGIN      
        1 agactgcgcc cagcagtgcc gccggccccc acccgtcgat gcctgcttac agggagccgg
       61 cagagcgcag acgcgcggtg acgtcacggg aggcgtccgg cgtaggcgaa accttttgaa
      121 tctagctggc gggagttgct gctgcagggc gagctgtgca gcaatggagt ccgcctcggc
      181 aagtgaggag aatgaaggag gtgcggcgat agaggagtgc gtgagtagga tcccggtacc
      241 aagaccaccc tccattgagg agttcaccat agtgaagccc attagccgtg gtgcattcgg
      301 gaaagtgtac ctgggacaga aaggcggcaa gttgtacgca gtgaaggttg tcaaaaaggc
      361 agacatgatc aataaaaata tgactcatca ggtacaagct gagagagatg ccctggcact
      421 gagcaaaagc ccttttgttg tgcatctcta ttactcactg cagtcagcaa gcaacatcta
      481 cttgataatg gagtatctta ttggtggaga tgtcaagtct ctcctacata tatatggtta
      541 ttttgatgaa gagatggcta taaaatatat ttctgaagta gcattggctt tagactacct
      601 gcacagacat ggaatcatcc atagggattt gaaaccggac aatatgctta tttccaatga
      661 gggtcatatt aaactgacag attttggcct ttccaaagtt actttgaata gagacatcaa
      721 tatgatggat attctcacaa caccatcaat gtctaaacct aagcaagatt attcaagaac
      781 tccaggacaa gtcttatctc tcatcagttc tttgggattt ttcacaccag ttggagaaaa
      841 agatcaagac tctgcaaaca tgttttcagc ccctaagtct gctgcacaac tttctcgagg
      901 attcatttgt cctatgtctg tagatcagaa ggagccgact tcttattcga gcaaactgct
      961 aaaatcatgt tttgaaaccc tttcatccaa tccagaaata cctgtgaaat gtctgacttc
     1021 taatctactc cagtgtagga aaagactggg tacctcaagt actagtagcc agtctcatac
     1081 cttcgtatcc agtgtggagt cagaatgcca cagcaatccc aaatgggaaa gagactgcca
     1141 gagtaccgaa tcatcaggct gtgcaatgag ctggaatgca gttgaaatgc tgtatgcaaa
     1201 atctacaagt gccatcaaga caaaaacaga actggaatta gcactttctc ccattcatga
     1261 cagcagtgcc attcctgctg ctggaagcaa ccaggtgacc ctccctagaa aatgcttcag
     1321 ggaaatctct tgggaagcaa gggacccaga taatgagaac atgaccattg acaagggaca
     1381 gtctggtttc tgtcagtcca gtcagaggtc tgtaaattct agtgctacat ctgaagaaca
     1441 ccttggtaaa agaaattata aaagaaattt ccacttggtt gactccagtc cttgtcagga
     1501 aattatgcaa agtaaaaaaa actgtacaga gtatgaggcc aataaggaaa ggcaaggctg
     1561 ccgtgcaaat caaagtacag gcttgacaac cgaagtccag aacctgaagc tatcagggtg
     1621 tgaaagtcag caacttgact atgccaataa ggagaacatt gtcacctatt taactgacag
     1681 acaaacccca gaaaaactgc atataccaac gatagcaaag aaccttatgt ctgaactaga
     1741 tgaagactgt gagctgagca gtaaaaagga ctgcctcagt tctaattctg tatgttctga
     1801 tgaggataga gctcttaaaa ctacctgtgt ggattcggat tcatcttttc ctggagtgtc
     1861 tatgatggag agttcactgg aaattcaggc cttagaacca gataaaagca tcagagacta
     1921 ctcttttgaa gaaccaaaca ctgaagatct atttgtgtta ccaaaatgcc aagaaaattc
     1981 cttgccacaa gatgactgcc atgcttgtat ccaagacagt agccaagtgt cagctcatcc
     2041 ctcaaaggcg cccaaagcat tgacctctaa aatcaacgtc gtggcttttc gaagttttaa
     2101 cagtcatatt aacgcatcta ctaattcgga accatccaaa atcagcataa cttctttaga
     2161 tgctatggac atttcctgtg attacagtgg ttcatacccc atggctgtga gccctactga
     2221 gaaaggaagg cactacacgt cacatcagac tccaaatcag gtcaagttgg gaacatcata
     2281 cagaactcca aagagtgtga gaagaggggc agccccagta gatgacgggc gaattctagg
     2341 gactccagat tacctggcac ctgagctctt actgggtaca gcccatggtc ctgcagtaga
     2401 ctggtgggct cttggagttt gcttgttcga atttctgaca ggaattcccc ctttcaatga
     2461 tgaaacacca caacaagtat tccagaatat tttaaaaaga gatatcccat ggccagaagg
     2521 cgaagaaaag ctatctgata atgctcaaag tgcaatggac atgcttttaa ccattgatga
     2581 ttcaaagaga gctggaatga gagaactaaa acagcatcct ctcttcagtg aagtggactg
     2641 ggaaaatctg cagcatcaga ctatgccttt cgtaccccaa ccagacgacg aaacagatac
     2701 atcctatttt gaagccagaa ataatgctca acatctgacc atatctgggt ttagtctgta
     2761 gcacatgcgt gtcattttta tctaacttgt gatatagaat taagttttac agtaatatgc
     2821 tacttaatac tagattggtc taaatgggat aaaagtcatt attttaccca gactgaacag
     2881 cttttaatta ctaagtacaa cagtttttac agaattaaaa tactataagc aatataatca
     2941 gtaattaatc tttaccttag aactgtatat aagccataat agcttttttc atcttattta
     3001 ttcactgcac tttatgaaga gcaaagtatc aataaactaa aacactacca ctctaaatag
     3061 agggagtgag ccgtaagctt atctttatct gctgatttta attctattca ctttggggtt
     3121 aatatcaaac caaaaaaaag gactatttca tttaaattta atttatttgc acatggttac
     3181 atgcaaagct gttcttcagg ctgtcatatg agacaaacat gactgaaaat catgtgaacc
     3241 agacatgatg gtggaggtcc ccatgatcag gagcagatgc aacacaggag tatgtctgca
     3301 acttgaagac taaggccagc caggactata ttactaagca ctgtctctgc agggtgtgga
     3361 ctagtcaaaa gaactaaggt tccagctcac cttactagtg atggagtctc actgccttga
     3421 atgctcttgt tcccctctac aattcctgct tctttaaatg ggtagtggaa agattgggtg
     3481 gttttaatag cttgttcact tttaatagaa aaatttatac tttatgtcat atcatttcaa
     3541 acatcataag agacaaatag taataatttc ttttgcttac aaaatgtaac tgtaattagt
     3601 gggttcagta tcattctttt aaagaatatg tattaaaact cagctcacaa gtcttatctt
     3661 aaactagttg agactttcaa tatttctcta gaagaggaaa attaagattt acatgtctca
     3721 tgggtaagat tttactaatg cccttgtgat actggccata gtgctttcat taacacctag
     3781 tcacaggcta tacagaatgt cgtttacaaa gatgcataat cagtgctgag tattgaatct
     3841 ttacttattt tagtaacgac tacttaatgt tccagtccct tcttactaaa aatattcaaa
     3901 atatattgac aacttcactt actatgactc actttcaggt aacagcataa ttctattcta
     3961 tttactcaac catcctctta gagtgttttc aagtactaga aagaaaaaag cttttggaat
     4021 caaataatgg ctaaatgatt gtagtgccaa actgaaatga tgtgggcagg ttaaaagtga
     4081 gcactaagat gctggaggct ggaggtagca attgcttagt gtgcaaaaaa ccttgtgttc
     4141 aatccccact aacacacaaa tattaattac tttgtagaat tagaataagc agaaccatat
     4201 taagaaacaa cctggggttc aagagtcctg gcctatgata tgagagcact ggaccccagc
     4261 tgtggggtaa aaatgaatct gcagaagtaa gcccctaatg gcttcttaaa tcaaatcatt
     4321 ggacaattgg ttatagtaaa attaaattct gctgtggatt attgagaggt cctggcctgc
     4381 cggggtctac atgaatatag aactgacata gtcagaaaag tattgtaaat ggatggctga
     4441 ataagcaggg aaacaatgtg ctatgtgtaa ggatacagaa gagaaggaag acaccatgta
     4501 tattttttgt aaaatgcatt tttctacttt ccctgtttct acagagtgaa tcataaatag
     4561 tttgcacatt tatgtttcat tttcttttat ttgtacagaa tgtgatttaa aaaaaaaaag
     4621 aattacttct cctttcaaga ttatttatcc ta
//
