LOCUS       NM_175439               2882 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus methionine-tRNA synthetase 2 (mitochondrial) (Mars2),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_175439
VERSION     NM_175439.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Cheong,A., Archambault,D., Degani,R., Iverson,E., Tremblay,K.D. and
            Mager,J.
  TITLE     Nuclear-encoded mitochondrial ribosomal proteins are required to
            initiate gastrulation
  JOURNAL   Development 147 (10) (2020)
   PUBMED   32376682
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2882)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 2882)
  AUTHORS   Spencer,A.C., Heck,A., Takeuchi,N., Watanabe,K. and Spremulli,L.L.
  TITLE     Characterization of the human mitochondrial methionyl-tRNA
            synthetase
  JOURNAL   Biochemistry 43 (30), 9743-9754 (2004)
   PUBMED   15274629
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122223.4.
            
            On Aug 30, 2006 this sequence version replaced NM_175439.2.
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2882              AC122223.4         164636-167517       c
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 28.08 cM"
     gene            1..2882
                     /gene="Mars2"
                     /gene_synonym="C730026E21Rik; MetRS"
                     /note="methionine-tRNA synthetase 2 (mitochondrial)"
                     /db_xref="GeneID:212679"
                     /db_xref="MGI:MGI:2444136"
     exon            1..2882
                     /gene="Mars2"
                     /gene_synonym="C730026E21Rik; MetRS"
                     /inference="alignment:Splign:2.1.0"
     CDS             64..1824
                     /gene="Mars2"
                     /gene_synonym="C730026E21Rik; MetRS"
                     /EC_number="6.1.1.10"
                     /note="methionyl-tRNA synthetase, mitochondrial; mtMetRS;
                     methionine--tRNA ligase 2; mitochondrial methionine--tRNA
                     ligase; methionyl-tRNA synthetase 2; mitochondrial
                     methionyl-tRNA synthetase; methionine--tRNA ligase,
                     mitochondrial"
                     /codon_start=1
                     /product="methionine--tRNA ligase, mitochondrial
                     precursor"
                     /protein_id="NP_780648.1"
                     /db_xref="CCDS:CCDS14963.1"
                     /db_xref="GeneID:212679"
                     /db_xref="MGI:MGI:2444136"
                     /translation="MLRQCARWVLTRTRFGRGCRRYGSCSPSASGDAGEARAYFTTPI
                     FYVNAAPHIGHLYSALLADALCRHRRLRVPGSASTRFSTGTDEHGLKIQQAAATAGLA
                     PIELCDRVSAQFLQLFREADISSTDFIRTTEARHRVAVQHFWGVLEARGLLYKGIYEG
                     WYCASDECFLPEAKVTRQVGPSGDPCPVSLESGHPVSWTKEENYIFKLSQFREPLQRW
                     LGNNPQAITPEPFHQAVLQWLEEELPDLSVSRRSSHLHWGIPVPGDDSQTIYVWLDAL
                     VNYLTVVGYPDADFKSWWPATSHIIGKDILKFHAIYWPALLLGAGLRPPHRIYVHSHW
                     TVSGQKMSKSLGNVVDPRTCLDRYTVDGFRYFLLRQGVPNWDCDYYDEKVVKLLDSEL
                     ADALGGLLNRCTAYRINPSGTYPSFCAACFPSEPGLTGPSVRVQAEDYALVTAVATLP
                     KLVAGYYNDFQIYKALEAVSSCVRQTNGFVQRHAPWKLNWESPEDAPWLGTVLHVALE
                     CLRVFGTLLQPVTPNLADKLLSRLGVSTTERGLGELYFLPRFYGHPCPFEGRKLGPDT
                     GLLFPRLDQSRTRLVKAHRT"
     regulatory      2853..2858
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mars2"
                     /gene_synonym="C730026E21Rik; MetRS"
                     /note="hexamer: ATTAAA"
     polyA_site      2873
                     /gene="Mars2"
                     /gene_synonym="C730026E21Rik; MetRS"
                     /note="major polyA site"
ORIGIN      
        1 ctgcgcacgc gccctttccc gtgctcggga acaccgcctc cagtgctggg gccggctgtc
       61 accatgctgc ggcagtgtgc tcgctgggtg ttgacgcgca cccggttcgg ccgcggctgt
      121 cgccgctacg gttcgtgctc gccgagcgct agtggcgacg ccggggaagc gcgggcctac
      181 ttcaccacac ctatcttcta cgtgaacgcc gcgccgcaca tcgggcacct gtactcggcg
      241 ctactggccg acgccctgtg ccgccaccgt cgtctccgag ttcccggctc tgcgtccacg
      301 cggttttcca ccggcacgga tgagcacggc ctgaagatcc agcaggcggc agccaccgcc
      361 ggcttggccc cgatcgagct gtgcgatcga gtgtctgccc agtttttgca gcttttccgg
      421 gaggctgaca tctcttccac ggacttcatc cgcaccacgg aggctcggca ccgagtagcg
      481 gtgcagcatt tctggggtgt gctggaggcc cggggtctgc tttacaaagg gatctatgaa
      541 gggtggtatt gtgcctcgga tgagtgcttc ttgccggagg ccaaggttac tcggcaggtg
      601 ggtccgtccg gggatccctg tcccgtgtct ctcgagagcg gacatcctgt ctcctggacg
      661 aaggaagaaa attacatttt caagctttct cagtttcgag aaccactcca gcggtggctt
      721 ggaaacaatc ctcaggcaat cacaccggaa ccgttccacc aggcagttct tcagtggctg
      781 gaggaggagc tgcccgactt gtccgtttct cgaaggagta gccatctgca ctggggcata
      841 ccagttcccg gggacgactc gcagaccatc tacgtgtggc tggatgccct ggtcaactac
      901 ctcactgtag ttggctaccc agatgcagat ttcaagtctt ggtggccagc cacctctcat
      961 atcataggta aggacattct caaatttcat gctatttatt ggcctgccct tctcctaggg
     1021 gcaggattga ggccaccaca tcgcatctat gtccactcgc actggacggt gagtggccaa
     1081 aaaatgtcca aaagcttggg caacgtggtg gatcccagga cttgccttga tcgctatact
     1141 gtggatggct ttcgctactt tctccttcgg cagggcgttc ccaattggga ctgcgattac
     1201 tatgatgaaa aggtggttaa attgctggac tccgagctgg cagatgcctt aggagggctt
     1261 ttaaaccggt gcactgccta cagaataaat ccttctggaa cctacccatc tttctgtgct
     1321 gcctgtttcc ccagtgagcc agggttaacg gggccgtcag ttcgtgtgca ggcagaagac
     1381 tatgcgcttg taactgcagt ggccacttta cccaaactgg tagcgggcta ttataatgac
     1441 tttcagatct ataaggctct ggaggcggta tccagctgtg ttcggcaaac taatggtttc
     1501 gtccaaaggc acgcaccatg gaaattgaac tgggagagcc ctgaggatgc tccctggctg
     1561 ggtaccgtgc ttcacgtagc cttggagtgc ttgcgagtgt ttggaacttt gcttcagcct
     1621 gttactccaa acttagcaga taaactgctg tcaagactgg gagtctctac cacagagaga
     1681 ggccttggag agctctactt cctacctcga ttctatggac acccatgccc ctttgagggg
     1741 agaaagttgg gacctgacac tgggcttttg tttcctagac tggaccaatc taggactcgg
     1801 ctagtgaaag cccacaggac ctaggaactc agctcttact gactggtagt aaaagatcaa
     1861 atgtattctt tttgcgtttt taagtaaagt catgcttaaa gagtatgtat caaatgagca
     1921 cataaactat gtccttatga acagaacgat tcaggtctct ctaacccagt ccttttcatt
     1981 tctcagtgcc attaatcact gttcttgctc accaatgtct ccaagagaag gatggaagat
     2041 gctgacactt ccttctcatt gcaccttctc ccaacccagg gtcagggctt ctgctcccca
     2101 ggctaattta acacagcccc ttcttcaact aaatctaggg ttggaagtcc ctgctctgtt
     2161 tttacatgtc agccctactt tgttaaacag agtctcctgt taaagaggtt tttttttttt
     2221 tgaactttag gaactagtgt cctagacttt tacccatgtc aacccagcag ttgtgctttg
     2281 taatactaca ctccatccag tagatggctc tgctcgcaca cattgagctc cttgagagtg
     2341 tcctttcaga agacagaatt attttcagct gtttaattca cagaatttta gaattgtact
     2401 gttcttaaag gataactttg gaaacactga tatttttaca acagaaggac actagaattc
     2461 taattttaaa actattaaag tgacatgtac aataggatat aagtaaatat gacaggttta
     2521 tattaaaaaa ctaaaaagct cttgaggtac actgtactac agataatagt aacagttaca
     2581 tgttcatcta taaagccctc aaaagggttg tattctttat attatttctt atccacatac
     2641 ataatttacc tcatgattat ttttggtcaa ccaggataca taatatagga aagatataat
     2701 attgtaatta gtgaatttat gtcaaaatag tttttgttga ttactctctt tgagacaagc
     2761 tttcactatg taatttgtta tcatcagtct cttgcttcca cttttcaggt gttgggatta
     2821 cagggtatgc caccatgcct gccttttaac ttattaaagt gtggttctaa gtaccttctt
     2881 ta
//
