LOCUS       NM_001003913            2948 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus methionine-tRNA synthetase 1 (Mars1), transcript
            variant 2, mRNA.
ACCESSION   NM_001003913 XM_125952
VERSION     NM_001003913.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Xiang,J., Pompetti,A.J., Faranda,A.P., Wang,Y., Novo,S.G., Li,D.W.
            and Duncan,M.K.
  TITLE     ATF4 May Be Essential for Adaption of the Ocular Lens to Its
            Avascular Environment
  JOURNAL   Cells 12 (22), 2636 (2023)
   PUBMED   37998373
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Furuya,S., Yoshida,K., Kawakami,Y., Yang,J.H., Sayano,T., Azuma,N.,
            Tanaka,H., Kuhara,S. and Hirabayashi,Y.
  TITLE     Inactivation of the 3-phosphoglycerate dehydrogenase gene in mice:
            changes in gene expression and associated regulatory networks
            resulting from serine deficiency
  JOURNAL   Funct Integr Genomics 8 (3), 235-249 (2008)
   PUBMED   18228065
REFERENCE   3  (bases 1 to 2948)
  AUTHORS   Kim,J.Y., Kang,Y.S., Lee,J.W., Kim,H.J., Ahn,Y.H., Park,H., Ko,Y.G.
            and Kim,S.
  TITLE     p38 is essential for the assembly and stability of macromolecular
            tRNA synthetase complex: implications for its physiological
            significance
  JOURNAL   Proc Natl Acad Sci U S A 99 (12), 7912-7916 (2002)
   PUBMED   12060739
REFERENCE   4  (bases 1 to 2948)
  AUTHORS   Ubeda,M., Schmitt-Ney,M., Ferrer,J. and Habener,J.F.
  TITLE     CHOP/GADD153 and methionyl-tRNA synthetase (MetRS) genes overlap in
            a conserved region that controls mRNA stability
  JOURNAL   Biochem Biophys Res Commun 262 (1), 31-38 (1999)
   PUBMED   10448063
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC114678.20, BC079643.1 and
            CJ278192.1.
            
            On Jan 21, 2010 this sequence version replaced NM_001003913.1.
            
            Summary: The encoded protein belongs to the class I family of tRNA
            synthetases, a class of enzymes that charge tRNAs with their
            cognate amino acids. The related human gene product is essential
            for the translation initiation of mRNAs. This gene has an
            overlapping 3' UTR tail-to-tail arrangement with an adjacent gene
            on the opposite strand that encodes an inhibitor of the
            CCAAT/enhancer-binding protein's DNA binding activity. This
            arrangement, conserved in human and mouse, may be involved in mRNA
            stability and possible functional and regulatory interaction of
            these adjacent overlapping genes. Alternative splicing results in
            multiple transcript variants.[provided by RefSeq, Jan 2010].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 3' coding region, compared to variant 1. The encoded
            protein (isoform 2) is shorter, compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC079643.1, SRR13422586.296702.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-147               AC114678.20        145664-145810
            148-2934            BC079643.1         1-2787
            2935-2948           CJ278192.1         724-737
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 74.5 cM"
     gene            1..2948
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /note="methionine-tRNA synthetase 1"
                     /db_xref="GeneID:216443"
                     /db_xref="MGI:MGI:1345633"
     exon            1..266
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     CDS             158..2866
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /EC_number="6.1.1.10"
                     /note="isoform 2 is encoded by transcript variant 2;
                     methionine tRNA ligase; methionyl-tRNA synthetase,
                     cytoplasmic; methionine--tRNA ligase, cytoplasmic"
                     /codon_start=1
                     /product="methionine--tRNA ligase, cytoplasmic isoform 2"
                     /protein_id="NP_001003913.1"
                     /db_xref="CCDS:CCDS24237.1"
                     /db_xref="GeneID:216443"
                     /db_xref="MGI:MGI:1345633"
                     /translation="MRLFVSEGSPGSLPVLAAAARARGRAELLISTVGPEECVVPFLT
                     RPKVPVLQLDSGNYLFSASAICRYFFLLCGWEQDDLTNQWLEWEATELQPVLSAALHC
                     LVVQGKKGEDILGPLRRVLTHIDHSLSRQNCPFLAGDTESLADIVLWGALYPLLQDPA
                     YLPEELGALQSWFQTLSTQEPCQRAAETVLKQQGVLALRLYLQKQPQPQPPPPEGRTV
                     SNELEEEELATLSEEDIVTAVAAWEKGLESLPPLKLQQHPVLPVPGERNVLITSALPY
                     VNNVPHLGNIIGCVLSADVFARYCRLRQWNTLYLCGTDEYGTATETKAMEEGLTPREI
                     CDKYHAIHADIYRWFGISFDTFGRTTTPQQTKITQDIFQRLLTRGFVLRDTVEQLRCE
                     RCARFLADRFVEGVCPFCGYEEARGDQCDRCGKLINAIELKKPQCKICRSCPVVRSSQ
                     HLFLDLPKLEKRLEDWLGKTVPGSDWTPNARFIIRSWLRDGLKPRCITRDLKWGTPVP
                     LEGFEDKVFYVWFDATIGYVSITANYTDQWEKWWKNPEQVDLYQFMAKDNVPFHGLVF
                     PCSVLGAEDNYTLVKHIIATEYLNYEDGKFSKSRGIGVFGDMAKDTGIPADIWRFYLL
                     YIRPEGQDSAFSWTDLLIKNNSELLNNLGNFINRAGMFVSKFFGGCVPEMALTPDDRR
                     LVAHVSWELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGGEMDRQRA
                     GTVTGMAVNMAALLSVMLQPYMPTVSSTIQTQLQLPPAACRILATSFICTLPAGHRIG
                     TVSPLFQKLENDQIENLRQRFGGGQAKGSPKPAAVEAVTAAGSQHIQTLTDEVTKQGN
                     VVRELKAQKADKNQVAAEVAKLLDLKKQLALAEGKPIETPKGKKKK"
     exon            267..357
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     exon            358..436
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     exon            437..571
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     exon            572..647
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     exon            648..826
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     exon            827..933
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     exon            934..1050
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1254
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     exon            1255..1456
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     exon            1457..1531
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..1702
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     exon            1703..1798
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     exon            1799..1916
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     exon            1917..2130
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     exon            2131..2262
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     exon            2263..2367
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     exon            2368..2554
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     exon            2555..2626
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     exon            2627..2719
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     exon            2720..2939
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2908..2913
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /note="hexamer: AATAAA"
     polyA_site      2934
                     /gene="Mars1"
                     /gene_synonym="Mars; Metrs; Mtrns"
                     /note="major polyA site"
ORIGIN      
        1 gtttcgtaat ggtgcgtgat gggatgagta gtcggctttc ctggagccta ccgcgagcgt
       61 ccggccgaca cagaactcca tttcccgacg tgccttgcag cttaacccag gagcgggagg
      121 ccggttccga ttgcatcagc gtgggctccg aggcacgatg agactgttcg tgagcgaggg
      181 ttccccgggg agcctgcccg tgctggctgc ggccgcgagg gcccggggtc gggcggagct
      241 gctcatcagc accgtaggcc ccgaagagtg tgtggtacca ttccttaccc ggcctaaggt
      301 ccctgtcttg cagctggata gtggcaacta cctcttctct gctagtgcaa tctgccgata
      361 tttttttctg ttatgtggct gggaacaaga tgatctcacc aaccagtggc tggaatggga
      421 ggcaacagaa ctgcagccag ttctgtctgc tgccctacac tgtctagtgg ttcaaggcaa
      481 gaaaggggaa gatatacttg gcccacttcg gagagtcctg actcacattg atcacagctt
      541 gagtcgtcaa aactgtcctt tcctggctgg ggacacagaa tctctagctg acattgtttt
      601 gtggggagca ctgtatcctt tactgcaaga cccagcttac ctccctgagg agttgggtgc
      661 cctgcaaagt tggttccaga cactgagtac ccaggaaccg tgtcagcgag ctgcagagac
      721 ggtgctaaaa cagcagggtg tcctggcact tcgtctgtac ctccagaaac agccacagcc
      781 tcagcccccg cctcctgagg ggagaactgt cagcaacgag ctggaggaag aggaactggc
      841 taccttgtct gaggaggaca tcgttacagc tgttgccgcg tgggagaagg gtctggaaag
      901 cctgcctccg ctaaagctcc agcagcatcc agtgttgcct gtgcctggag agaggaatgt
      961 tctcatcacc agtgccctcc cctatgtcaa caatgtcccc caccttggaa acatcattgg
     1021 ctgtgtgctc agtgctgatg tctttgcaag gtattgtcgc cttcgccagt ggaataccct
     1081 ctatctgtgt ggtacagatg agtatggtac tgcgacagag accaaggcca tggaggaggg
     1141 cctaacccca cgggaaatct gtgacaagta ccatgccatc catgctgaca tctaccgctg
     1201 gttcggcata tcgttcgata ctttcgggcg cactaccact cctcagcaga ccaaaatcac
     1261 ccaggacatc ttccagaggt tgctgacccg ggggtttgtg ctgcgagata ctgtggagca
     1321 gcttcggtgt gagcggtgtg cacgtttcct ggctgaccgc tttgtggagg gtgtgtgtcc
     1381 cttctgtggc tatgaagagg cccgaggtga ccagtgtgac aggtgtggca agctcatcaa
     1441 tgccattgag ctcaagaaac cacagtgcaa aatctgccgc tcctgccctg tggtgaggtc
     1501 ctcacagcac ctgtttctag acttgcctaa gttggaaaag cgtctggagg actggttggg
     1561 gaagacagtg cctggcagtg actggacacc caatgccagg ttcattatac gttcctggct
     1621 tcgagatggc ctcaagccac gatgcatcac cagagacctc aaatggggaa cgcctgtgcc
     1681 cttggaaggt tttgaggaca aggtatttta cgtctggttt gatgctacta ttggctacgt
     1741 gtccatcaca gccaactaca cagaccaatg ggagaaatgg tggaagaacc cagaacaagt
     1801 ggacctttac cagttcatgg ccaaagacaa tgttcccttc catggcttgg tctttccgtg
     1861 ttcagtccta ggagctgagg acaactacac cctggtcaag cacatcattg ctacagagta
     1921 cctgaactat gaggatggga aattctctaa gagccggggc ataggagtgt ttggagacat
     1981 ggccaaggat acaggaatcc ctgctgacat ctggcgattc tatctgctat acattcggcc
     2041 tgagggccag gacagtgcct tctcctggac agacttgttg attaaaaaca attctgagct
     2101 gctcaacaac ctgggcaact tcatcaacag agctggcatg tttgtttcta agttttttgg
     2161 cggttgtgtg cctgagatgg cgctaacccc tgatgacaga cgcctggtgg cccatgtctc
     2221 ttgggaactc cagcactatc accagctgtt ggaaaaggtt cggatccggg atgccttgcg
     2281 cagtatcctc accatatctc gccatggcaa ccaatacatt caagtgaatg agccctggaa
     2341 acggattaaa ggtggtgaga tggacaggca gcgggcaggc acagtgacag gcatggcagt
     2401 gaacatggct gccttgctgt ctgtcatgct gcagccatac atgcccacag tcagctctac
     2461 catccagacc cagctgcagc tcccacctgc agcctgccgc atccttgcca caagcttcat
     2521 ttgtaccttg ccagcaggcc accgaattgg cacagtcagt cctttgttcc aaaaactgga
     2581 aaatgaccag attgaaaatt tgaggcagcg ctttggaggg ggtcaggcta aaggctcccc
     2641 caagccagca gctgtggagg cagttacagc agcaggctcg cagcacatac aaacgctgac
     2701 ggatgaggtg accaagcagg gcaacgtcgt ccgggaactg aaagcacaga aggcagacaa
     2761 gaaccaggtt gctgcagagg tggctaaact cttggatcta aagaaacagt tggctttggc
     2821 tgaggggaaa cccattgaaa ctcctaaagg caagaagaaa aagtgagagg ctgttgacac
     2881 aaagtcactt tactggacat ggacagtaat aaacaatgta ccgtctatgt gcaagccgaa
     2941 aaaaaaaa
//
