LOCUS       NM_172279               3581 bp    mRNA    linear   ROD 03-AUG-2024
DEFINITION  Mus musculus MAP/microtubule affinity regulating kinase 4 (Mark4),
            transcript variant 1, mRNA.
ACCESSION   NM_172279
VERSION     NM_172279.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Wu,Y., Zhang,J., Wang,W., Wu,D., Kang,Y. and Fu,L.
  TITLE     MARK4 aggravates cardiac dysfunction in mice with STZ-induced
            diabetic cardiomyopathy by regulating ACSL4-mediated myocardial
            lipid metabolism
  JOURNAL   Sci Rep 14 (1), 12978 (2024)
   PUBMED   38839927
  REMARK    GeneRIF: MARK4 aggravates cardiac dysfunction in mice with
            STZ-induced diabetic cardiomyopathy by regulating ACSL4-mediated
            myocardial lipid metabolism.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Sultanakhmetov,G., Limlingan,S.J.M., Fukuchi,A., Tsuda,K.,
            Suzuki,H., Kato,I., Saito,T., Weitemier,A.Z. and Ando,K.
  TITLE     Mark4 ablation attenuates pathological phenotypes in a mouse model
            of tauopathy
  JOURNAL   Brain Commun 6 (3), fcae136 (2024)
   PUBMED   38712317
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3581)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 3581)
  AUTHORS   Yu,X., Chen,X., Amrute-Nayak,M., Allgeyer,E., Zhao,A.,
            Chenoweth,H., Clement,M., Harrison,J., Doreth,C., Sirinakis,G.,
            Krieg,T., Zhou,H., Huang,H., Tokuraku,K., Johnston,D.S., Mallat,Z.
            and Li,X.
  TITLE     MARK4 controls ischaemic heart failure through microtubule
            detyrosination
  JOURNAL   Nature 594 (7864), 560-565 (2021)
   PUBMED   34040253
  REMARK    GeneRIF: MARK4 controls ischaemic heart failure through microtubule
            detyrosination.
REFERENCE   5  (bases 1 to 3581)
  AUTHORS   Yang,K., Cai,J., Pan,M., Sun,Q. and Sun,C.
  TITLE     Mark4 Inhibited the Browning of White Adipose Tissue by Promoting
            Adipocytes Autophagy in Mice
  JOURNAL   Int J Mol Sci 21 (8), 2752 (2020)
   PUBMED   32326642
  REMARK    GeneRIF: Mark4 Inhibited the Browning of White Adipose Tissue by
            Promoting Adipocytes Autophagy in Mice.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3581)
  AUTHORS   Magnani,I., Novielli,C., Fontana,L., Tabano,S., Rovina,D.,
            Moroni,R.F., Bauer,D., Mazzoleni,S., Colombo,E.A., Tedeschi,G.,
            Monti,L., Porta,G., Bosari,S., Frassoni,C., Galli,R., Bello,L. and
            Larizza,L.
  TITLE     Differential signature of the centrosomal MARK4 isoforms in glioma
  JOURNAL   Anal Cell Pathol (Amst) 34 (6), 319-338 (2011)
   PUBMED   22156016
  REMARK    GeneRIF: The balance between the MARK4 isoforms is carefully
            guarded during neural differentiation but may be subverted in
            gliomagenesis.
REFERENCE   7  (bases 1 to 3581)
  AUTHORS   Schneider,A., Laage,R., von Ahsen,O., Fischer,A., Rossner,M.,
            Scheek,S., Grunewald,S., Kuner,R., Weber,D., Kruger,C.,
            Klaussner,B., Gotz,B., Hiemisch,H., Newrzella,D.,
            Martin-Villalba,A., Bach,A. and Schwaninger,M.
  TITLE     Identification of regulated genes during permanent focal cerebral
            ischaemia: characterization of the protein kinase 9b5/MARKL1/MARK4
  JOURNAL   J Neurochem 88 (5), 1114-1126 (2004)
   PUBMED   15009667
  REMARK    GeneRIF: MARK4 is a functional protein kinase which specifically
            phosphorylates a cognate peptide substrate for the AMP-kinase
            family. On overexpression in heterologous cells functional
            wild-type protein, but not its kinase-dead mutant, decreased cell
            viability
REFERENCE   8  (bases 1 to 3581)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
REFERENCE   9  (bases 1 to 3581)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 3581)
  AUTHORS   Lee,Y.J., Park,C.W., Hahn,Y., Park,J., Lee,J., Yun,J.H., Hyun,B.
            and Chung,J.H.
  TITLE     Mit1/Lb9 and Copg2, new members of mouse imprinted genes closely
            linked to Peg1/Mest(1)
  JOURNAL   FEBS Lett 472 (2-3), 230-234 (2000)
   PUBMED   10788617
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC149052.3 and AC118017.15.
            
            On Feb 13, 2019 this sequence version replaced NM_172279.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY151083.1, SRR7345562.3477673.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164141 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-400               AC149052.3         187353-187752
            401-601             AC118017.15        2759-2959
            602-655             AC118017.15        5857-5910
            656-704             AC118017.15        6041-6089
            705-770             AC118017.15        6768-6833
            771-841             AC118017.15        7264-7334
            842-898             AC118017.15        9350-9406
            899-1135            AC118017.15        11162-11398
            1136-1255           AC118017.15        16997-17116
            1256-1355           AC118017.15        17478-17577
            1356-1465           AC118017.15        19594-19703
            1466-1625           AC118017.15        19799-19958
            1626-1843           AC118017.15        20979-21196
            1844-1947           AC118017.15        22643-22746
            1948-2226           AC118017.15        24911-25189
            2227-2271           AC118017.15        26397-26441
            2272-3581           AC118017.15        28104-29413
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 9.78 cM"
     gene            1..3581
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /note="MAP/microtubule affinity regulating kinase 4"
                     /db_xref="GeneID:232944"
                     /db_xref="MGI:MGI:1920955"
     exon            1..400
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /inference="alignment:Splign:2.1.0"
     CDS             350..2608
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     MAP/microtubule affinity-regulating kinase 4L; MARK4
                     serine/threonine protein kinase"
                     /codon_start=1
                     /product="MAP/microtubule affinity-regulating kinase 4
                     isoform 1"
                     /protein_id="NP_758483.1"
                     /db_xref="CCDS:CCDS20903.1"
                     /db_xref="GeneID:232944"
                     /db_xref="MGI:MGI:1920955"
                     /translation="MSSRTALAPGNDRNSDTHGTLGSGRSSDKGPSWSSRSLGARCRN
                     SIASCPEEQPHVGNYRLLRTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPSSLQK
                     LFREVRIMKGLNHPNIVKLFEVIETEKTLYLVMEYASAGEVFDYLVSHGRMKEKEARA
                     KFRQIVSAVHYCHQKNIVHRDLKAENLLLDAEANIKIADFGFSNEFTLGSKLDTFCGS
                     PPYAAPELFQGKKYDGPEVDIWSLGVILYTLVSGSLPFDGHNLKELRERVLRGKYRVP
                     FYMSTDCESILRRFLVLNPAKRCTLEQIMKDKWINIGYEGEELKPYTEPEEDFGDTKR
                     IEVMVGMGYTREEIKEALTNQKYNEVTATYLLLGRKTEEGGDRGAPGLALARVRAPSD
                     TTNGTSSSKGSSHNKGQRASSSTYHRQRRHSDFCGPSPAPLHPKRSPTSTGDTELKEE
                     RMPGRKASCSAVGSGSRGLPPSSPMVSSAHNPNKAEIPERRKDSTSTPNNLPPSMMTR
                     RNTYVCTERPGSERPSLLPNGKENSSGTSRVPPASPSSHSLAPPSGERSRLARGSTIR
                     STFHGGQVRDRRAGSGSGGGVQNGPPASPTLAHEAAPLPSGRPRPTTNLFTKLTSKLT
                     RRVTDEPERIGGPEVTSCHLPWDKTETAPRLLRFPWSVKLTSSRPPEALMAALRQATA
                     AARCRCRQPQPFLLACLHGGAGGPEPLSHFEVEVCQLPRPGLRGVLFRRVAGTALAFR
                     TLVTRISNDLEL"
     exon            401..601
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /inference="alignment:Splign:2.1.0"
     exon            602..655
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /inference="alignment:Splign:2.1.0"
     exon            656..704
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /inference="alignment:Splign:2.1.0"
     exon            705..770
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /inference="alignment:Splign:2.1.0"
     exon            771..841
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /inference="alignment:Splign:2.1.0"
     exon            842..898
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /inference="alignment:Splign:2.1.0"
     exon            899..1135
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1255
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1256..1355
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1356..1465
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1466..1625
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1626..1843
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1844..1947
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1948..2226
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /inference="alignment:Splign:2.1.0"
     exon            2227..2271
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /inference="alignment:Splign:2.1.0"
     exon            2272..3581
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3195..3200
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /note="hexamer: AATAAA"
     polyA_site      3218
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /note="major polyA site"
     regulatory      3527..3532
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /note="hexamer: AATAAA"
     polyA_site      3578
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
     polyA_site      3581
                     /gene="Mark4"
                     /gene_synonym="2410090P21Rik; Markl1"
                     /note="major polyA site"
ORIGIN      
        1 gtcccttcct ccccggcccc ttggtcgcct ccctccgccg ccgccccggg ccggctccgc
       61 gccccctccc cggcccccgc ccgtgcaccc tcccgcctgc ccgccgccca gatggcgccc
      121 cgggttcccc ttgcacgggg ccactaggac cctcggtgtc ttctcccctc ccccgtcctg
      181 ccctctcttg ctctcgctct gcgaggactc gggcgtccac ggcgcccagc ttttgagctc
      241 gcgtccccag gtcggctggg gggggggagg ggaagagagg ggaccccggg atccccaccc
      301 cccccgcccg gccgcccctg tccctttccc ttctgggacc ccagagaaga tgtcttcgag
      361 gacggcgctg gccccgggca acgatcgaaa ctcggacacg catggcacat tgggcagtgg
      421 acgatcttcg gacaaagggc cgtcctggtc cagccgttcc ctgggtgccc gttgccggaa
      481 ctctatcgct tcctgccctg aggaacaacc ccatgtgggc aactataggc tgctaaggac
      541 catcgggaag ggcaacttcg ccaaagtcaa gctggctcgg catatcctca cgggccggga
      601 ggtcgctatt aagatcattg ataagaccca gctgaacccc agtagcttgc agaagctgtt
      661 cagagaagtc cgaattatga agggactcaa ccaccccaac atcgtgaagc tttttgaggt
      721 gatagagacg gagaagacgc tatacctggt gatggaatac gctagcgcag gagaagtgtt
      781 tgactacctc gtgtcgcacg gccgcatgaa ggagaaggag gctcgagcca agttccggca
      841 gatcgtgtca gccgtgcact actgtcatca gaagaacatt gtacacaggg atctaaaggc
      901 tgaaaacctg ttgctggatg ccgaggccaa catcaaaatc gccgacttcg gcttcagcaa
      961 tgagttcacg ctgggctcca agctggacac cttctgtggg agccccccat acgccgcccc
     1021 agagctgttc cagggcaaga agtatgatgg gccagaggtg gacatctgga gcctgggtgt
     1081 catcctgtac acgctggtca gcggctccct gcccttcgat gggcacaacc tcaaggagct
     1141 gcgggagcga gtcctcagag gaaagtaccg ggtccccttc tacatgtcta cagactgcga
     1201 gagcattctg cggagatttc tggtgctgaa ccccgcaaaa cgctgtactc tggagcaaat
     1261 catgaaagac aaatggatca acatcggcta tgagggtgag gagctgaagc catacacgga
     1321 gcctgaggag gacttcgggg acaccaagag aattgaggtg atggtgggta tgggctacac
     1381 acgggaagaa atcaaagagg ccttgaccaa ccagaagtac aacgaggtga ccgccaccta
     1441 cctcctgctg ggcaggaaga ctgaggaggg tggggaccgg ggtgccccag ggctggccct
     1501 ggcacgggtg cgggcgccca gcgacaccac caacgggaca agctccagca aaggcagcag
     1561 ccacaacaaa gggcaacggg cttcttcctc cacctaccac cgccagcgcc gtcacagtga
     1621 cttctgtggc ccgtcccctg ccccgctgca cccgaagcgc agcccaacca gcacgggaga
     1681 cacggagctc aaagaagagc ggatgccggg tcggaaagcg agctgcagtg cagtgggcag
     1741 tggaagtcga ggcttgcccc cctccagccc catggtcagc agtgcccaca accccaataa
     1801 ggcagagatc cctgagcggc ggaaggacag cactagcacc cctaacaacc tcccccccag
     1861 catgatgacc cgaagaaaca cctatgtgtg cacagagcga ccaggatctg aacgcccgtc
     1921 cttgttgcca aatggcaaag aaaatagctc cggtacctcg cgggtgcccc ctgcctcgcc
     1981 ttccagtcat agcctggctc ccccgtcagg cgagcggagc cgcctggctc ggggctccac
     2041 catccgcagc accttccatg ggggccaggt ccgagaccgg cgggcaggga gcgggagtgg
     2101 cgggggtgtg cagaatggac ccccagcctc acccacgctt gcccacgagg ccgcacccct
     2161 gccctccggg cggcctcgcc ccaccaccaa cctcttcacc aagctgacct ccaaactgac
     2221 ccgaagggtc acagacgaac ctgagagaat cgggggacct gaggtcacaa gttgccatct
     2281 accttgggat aaaacggaaa ccgcccccag gctgctccga ttcccctgga gtgtgaagct
     2341 gaccagctcg cgacctcctg aggccctgat ggctgccctg cgacaggcca cagcggccgc
     2401 ccgctgccgg tgccgccagc cgcagccgtt cctgctggcc tgcctgcacg ggggtgcggg
     2461 cgggcccgag cccctgtccc atttcgaagt ggaggtgtgc cagctgcccc ggcccggcct
     2521 caggggcgtc ctcttccgcc gtgtggcggg caccgccctg gccttccgca ccctcgtcac
     2581 ccgcatctcc aacgacctcg aactctgagc caccgccacc actaccaccg ccacagccac
     2641 catcacagcc cgggtccctt ctttctctgg ttcctttcac ttccccaaga ggggaagagg
     2701 acagaggaga gggtgccctg tgtcatgact gaagtttccc tggattagat tggtggacag
     2761 agacagtgtg gggacacatg acatgataag agggctcagc agggggagct ggcaccctcc
     2821 tagggcctct ggtgggaccc ccctccccac aatcttgttc ttctgcaggg cacctgagga
     2881 gactttgggg acaggagtga gaagggaaac tgaggaaatt ctcccattca gggagagctg
     2941 ccaggattaa tgactggaga cagacttggg gggttcaggg agttggggga gtcacagaca
     3001 gaaaccttcc cctcactccc ccttatgatc gaacctcctt catgccccag gctggcgcgg
     3061 ggcactttgt acaaatccgt gtatatactc ctgtccctct gcagaggtct ctcggggagc
     3121 tgctgctgcc gcctccgatt tttaagttat tgccccgccc cttctgtcag ctcctcatct
     3181 gcagcctgtt actcaataaa cagtaggagt ccctccaacc ccgacctcct ccctggccga
     3241 cctggggttt cccttctcag cccttggcct gcaggtgagc cagggagctg gggacttgac
     3301 cccaacctgt ggttctgctt gctgagcctt tgttatctca tcttcagaat gggaacagtg
     3361 gggttggagg atgggtcaag gatgactatg gaagagggca gaacagagct cagcctcttc
     3421 cacgaggccc cagccttctg tgacaccctc ctcttggcca ctcactcccc tctgccatat
     3481 tacactggac ccagagcctc ttcctattcc agtaatacat gtattcaata aacaatcaac
     3541 gactggtgcc gactccacgc taggcccagt tctggacata a
//
