LOCUS       NM_008538               4186 bp    mRNA    linear   ROD 05-DEC-2024
DEFINITION  Mus musculus myristoylated alanine rich protein kinase C substrate
            (Marcks), mRNA.
ACCESSION   NM_008538
VERSION     NM_008538.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Thowfeequ,S., Fiorentino,J., Hu,D., Solovey,M., Ruane,S.,
            Whitehead,M., Zhou,F., Godwin,J., Mateo-Otero,Y.,
            Vanhaesebroeck,B., Scialdone,A. and Srinivas,S.
  TITLE     An integrated approach identifies the molecular underpinnings of
            murine anterior visceral endoderm migration
  JOURNAL   Dev Cell 59 (17), 2347-2363 (2024)
   PUBMED   38843837
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4186)
  AUTHORS   Yang,Y., Yang,H. and Yang,C.
  TITLE     Circ-AMOTL1 enhances cardiac fibrosis through binding with EIF4A3
            and stabilizing MARCKS expression in diabetic cardiomyopathy
  JOURNAL   Cell Signal 111, 110853 (2023)
   PUBMED   37586467
  REMARK    GeneRIF: Circ-AMOTL1 enhances cardiac fibrosis through binding with
            EIF4A3 and stabilizing MARCKS expression in diabetic
            cardiomyopathy.
REFERENCE   4  (bases 1 to 4186)
  AUTHORS   Ji,S., Yang,Z., Gozali,L., Kenney,T., Kocabas,A., Jinsook Park,C.
            and Hynes,M.
  TITLE     Distinct expression of select and transcriptome-wide isolated
            3'UTRs suggests critical roles in development and transition states
  JOURNAL   PLoS One 16 (5), e0250669 (2021)
   PUBMED   33951080
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4186)
  AUTHORS   Yang,P., Xu,C., Reece,E.A., Chen,X., Zhong,J., Zhan,M.,
            Stumpo,D.J., Blackshear,P.J. and Yang,P.
  TITLE     Tip60- and sirtuin 2-regulated MARCKS acetylation and
            phosphorylation are required for diabetic embryopathy
  JOURNAL   Nat Commun 10 (1), 282 (2019)
   PUBMED   30655546
  REMARK    GeneRIF: Study in mice reports that Marcks is regulated by
            acetylation through Tip60 and Sirt2. Maternal diabetes-induced
            MARCKS acetylation is required for its phosphorylation, which
            disables the protective effects of MARCKS on mitochondria and the
            endoplasmic reticulum, leading to cellular organelle stress and
            neural tube defect formation.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4186)
  AUTHORS   Herget,T., Brooks,S.F., Broad,S. and Rozengurt,E.
  TITLE     Relationship between the major protein kinase C substrates acidic
            80-kDa protein-kinase-C substrate (80K) and myristoylated
            alanine-rich C-kinase substrate (MARCKS). Members of a gene family
            or equivalent genes in different species
  JOURNAL   Eur J Biochem 209 (1), 7-14 (1992)
   PUBMED   1396720
REFERENCE   7  (bases 1 to 4186)
  AUTHORS   Li,J. and Aderem,A.
  TITLE     MacMARCKS, a novel member of the MARCKS family of protein kinase C
            substrates
  JOURNAL   Cell 70 (5), 791-801 (1992)
   PUBMED   1516135
REFERENCE   8  (bases 1 to 4186)
  AUTHORS   Blackshear,P.J., Tuttle,J.S., Oakey,R.J., Seldin,M.F., Chery,M.,
            Philippe,C. and Stumpo,D.J.
  TITLE     Chromosomal mapping of the human (MACS) and mouse (Macs) genes
            encoding the MARCKS protein
  JOURNAL   Genomics 14 (1), 168-174 (1992)
   PUBMED   1427822
REFERENCE   9  (bases 1 to 4186)
  AUTHORS   Blackshear,P.J., Verghese,G.M., Johnson,J.D., Haupt,D.M. and
            Stumpo,D.J.
  TITLE     Characteristics of the F52 protein, a MARCKS homologue
  JOURNAL   J Biol Chem 267 (19), 13540-13546 (1992)
   PUBMED   1618855
REFERENCE   10 (bases 1 to 4186)
  AUTHORS   Brooks,S.F., Herget,T., Erusalimsky,J.D. and Rozengurt,E.
  TITLE     Protein kinase C activation potently down-regulates the expression
            of its major substrate, 80K, in Swiss 3T3 cells
  JOURNAL   EMBO J 10 (9), 2497-2505 (1991)
   PUBMED   1868832
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB569984.1, M60474.1, BC046601.1 and AC133944.4.
            
            On Sep 1, 2006 this sequence version replaced NM_008538.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M60474.1, BC046601.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                BB569984.1         1-31
            32-39               M60474.1           21-28
            40-1971             BC046601.1         1-1932
            1972-4186           AC133944.4         90450-92664
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 19.49 cM"
     gene            1..4186
                     /gene="Marcks"
                     /gene_synonym="Macs; PKCSL"
                     /note="myristoylated alanine rich protein kinase C
                     substrate"
                     /db_xref="GeneID:17118"
                     /db_xref="MGI:MGI:96907"
     exon            1..493
                     /gene="Marcks"
                     /gene_synonym="Macs; PKCSL"
                     /inference="alignment:Splign:2.1.0"
     CDS             392..1321
                     /gene="Marcks"
                     /gene_synonym="Macs; PKCSL"
                     /note="80K protein"
                     /codon_start=1
                     /product="myristoylated alanine-rich C-kinase substrate"
                     /protein_id="NP_032564.1"
                     /db_xref="CCDS:CCDS23784.1"
                     /db_xref="GeneID:17118"
                     /db_xref="MGI:MGI:96907"
                     /translation="MGAQFSKTAAKGEATAERPGEAAVASSPSKANGQENGHVKVNGD
                     ASPAAAEPGAKEELQANGSAPAADKEEPASGSAATPAAAEKDEAAAATEPGAGAADKE
                     AAEAEPAEPSSPAAEAEGASASSTSSPKAEDGAAPSPSSETPKKKKKRFSFKKSFKLS
                     GFSFKKSKKESGEGAEAEGATAEGAKDEAAAAAGGEGAAAPGEQAGGAGAEGAAGGEP
                     REAEAAEPEQPEQPEQPAAEEPQAEEQSEAAGEKAEEPAPGATAGDASSAAGPEQEAP
                     AATDEAAASAAPAASPEPQPECSPEAPPAPTAE"
     exon            494..4186
                     /gene="Marcks"
                     /gene_synonym="Macs; PKCSL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2599..2604
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Marcks"
                     /gene_synonym="Macs; PKCSL"
                     /note="hexamer: AATAAA"
     polyA_site      2609
                     /gene="Marcks"
                     /gene_synonym="Macs; PKCSL"
                     /note="major polyA site"
     regulatory      4034..4039
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Marcks"
                     /gene_synonym="Macs; PKCSL"
                     /note="hexamer: AATAAA"
     polyA_site      4057
                     /gene="Marcks"
                     /gene_synonym="Macs; PKCSL"
ORIGIN      
        1 gcacttgggc gttggacccc gcatcttatt agcaaccagg gagatttctc cattttcctc
       61 ttgtctacag tgcggctaca aatctgggat ttttttttat tacttctttt tttaaaaaaa
      121 ctacacttgg gctccttttt tgtgctcgac ttttccacct ttttccctcc ttcctgcgct
      181 gctgcttttt tgatctcttc gactaaaaat tttttatccg gagtatttaa tcgggtctct
      241 tctgtcctcc tcgccacccc caccccctcc ctccggtgtg tgtgccgccg ccgctgttgc
      301 tgctgctgct gctcgccccg tcgttacacc aaccgaaggc tctttgtttc ctctcttgga
      361 tctgttgagt ttctttgttg aagaagccag catgggtgcc cagttctcca agaccgcagc
      421 gaagggagaa gccaccgccg agaggcccgg ggaggcggct gtggcctcgt cgccttccaa
      481 agcaaatggg caggagaatg gccacgtaaa agtgaacggg gacgcgtctc ccgccgccgc
      541 cgagccgggc gccaaggagg agctgcaagc caacggcagc gccccggccg ccgacaagga
      601 ggagcccgcg agcggcagtg ccgcgacccc cgccgcggcc gaaaaggatg aggctgccgc
      661 ggccaccgag ccgggcgccg gcgcggccga caaggaggct gcggaggccg agcccgccga
      721 gcccagctcc ccggccgccg aggccgaggg cgcgtccgcc tcctccacgt cgtcgcccaa
      781 ggcggaggac ggggccgcgc cgtcgcccag cagcgagacc ccgaaaaaaa aaaagaagcg
      841 cttttccttc aagaagtcct tcaagctgag cggcttctcc ttcaagaaga gcaagaagga
      901 gtcgggcgag ggcgctgaag cagagggagc gaccgcggaa ggcgccaagg acgaggctgc
      961 agccgcagcg ggcggcgagg gggccgcggc ccccggggag caggcaggcg gggcgggcgc
     1021 cgagggcgcg gcgggcggag agccccggga ggccgaggcg gcggagcccg agcagccgga
     1081 gcagccggag cagcccgcgg cggaggagcc gcaggcggag gagcagtcgg aggcggcggg
     1141 ggagaaggcg gaggagcccg cgcccggcgc caccgcgggc gatgcgtcct ccgccgcagg
     1201 gcctgagcag gaggcgcccg ctgccaccga cgaggccgcg gcgtccgcag cccccgccgc
     1261 gtcgccggag ccgcagcccg agtgcagtcc ggaggcgccc cccgcgccaa cggccgagta
     1321 agctccagag ccctcacgca attcaagaac ttttcccccc agtttgtttg ttggagtggt
     1381 gccaggtact ggttttggag aacttgtcta caaccaggga ttgattttaa agattttttt
     1441 ttaaatttca catttttttt aagcagcaaa ttttttgttt tttgtttttt ttaagccccc
     1501 ttccccacag atcccatctc agatagttgt ttccaccatt ccgacaggcc gaggacgtgt
     1561 tagacagctt cctctgcctt ctttctttac ttttactttt tttttttttt tttggcatca
     1621 gtattaatgt tttttgcata ctttgcatct ttattaaaaa agtgtaaact ttctttgtca
     1681 gatctataga catacccata tatgaaggag atgggtgggt caaaaggaat aacaaatgaa
     1741 gtgatagggg ccactatggg aaattgaagc agtgcataac attgccaaga taatatgcca
     1801 ctaaaatggt ggtgggtgta aaggcttagg gttcttgtcc tttctttctt tctttctttc
     1861 tttctttctt tctttctttc tttttttttt taaagaaaaa ttattacgat gtattttgtg
     1921 aggcaggttt acaacactac acgttttgaa taagaaggaa agagaaaaaa ataaaaacac
     1981 caatacccag atttaaaaaa aaaaaaaaag atcatagtct taggagttca tgtaaaccat
     2041 aggaacttcc tgcttatctc atgttagctg taccagtcag tgattaagta gaactacaag
     2101 ttgtataggc tttattgttt attgctggtt tatgacctta ataaagtgta attatgtatt
     2161 accagcaggg tgtttttaac tgtgactatt gtataaaaac aaatcttgat atccttcaga
     2221 agcacatgaa gtttgcaagt ctccaccctg cccatttttg taaaactgca gtcatcttgg
     2281 accttttaaa cacaaaattt taaactcaac caagctgtga tacgtggaat ggttactgtt
     2341 tatactgtgg tatgtttttt gattacagca gacaatgctt tcctttccag ttgtctttga
     2401 gaataaagga gaaacagatc ttcagatgca atggttttgt gtagcatctc gtctctcgtg
     2461 ttttgtaaat actggaggag ctttgaccaa tttgacatag agctggaatg taacgttgct
     2521 tacaagcatt gctattcaac tcctgcttaa ggtgttctaa ttttctgtga gcacactaaa
     2581 agcaaaaaaa taaatgtgaa taaaatgtac aaatttgttg tgttttttta tgttctgagg
     2641 ctgacttcta ggtcttagat taatttttta ggaaggcctt gcatgccatc aaaagtacat
     2701 ttggctgtgg tttttgataa gaagttttca agctcagaaa ttcataatca gtgtcaaatt
     2761 aagtatgtag gaagatctta tactgtgtca gttgttacca tttacctgct tttagttata
     2821 gcatgcgaga ataagtcctt taagacaaaa gtggcttgac cctaagactt tcaaatgtct
     2881 tatgagacta tttcataaac tgtttccttg tcattgcagg tccagaaagt tacattttat
     2941 aatcaggaag aatgtggagt tcagactgca cataagtcat gaaaagatgg ccaaacctgt
     3001 ttaaatgttg atggtttatg tagtataagc cttagcgttt ggctcgtttc caaagtgttc
     3061 ttgaactgat atgctatact tcatggacag gtttctataa ggaagtaatg gtgttgaatg
     3121 gacttattgt cagtttgggc cattagcata gctgcaagct tggttttgtt tttgtttttt
     3181 agtcaaagtg ttagcatctt ttattcaaat gaaaagataa tcctttatat attaactctt
     3241 actagtttac tttctgctta ttttccatct cataactgga tatttttact ttactggacc
     3301 ctaaactgaa ttctttttat tttttaccat tgctttatta aatgcaaaat agtctacctg
     3361 ttgtgctctt ttcttgagta gagtgcaggg tggagttcag ggtaaagttg aaccactctg
     3421 gtggcacctc aatattacat cgtattaaaa aaagagataa cagcattagc ctttcaataa
     3481 tgttcctcta attgaaataa tagatcaaaa ttaatggata cttttctttc taaatatcct
     3541 ccctgataat aaaaccaagt aacacttgaa agaataaact tgaccctgag tttcttgagg
     3601 ttttggaaga ttaaagtcaa acctggtaac caaaggactg agtggtgggt cgttcccgct
     3661 ttaaccaact tgtttgagaa cccctagttg gccagatatg gtgcctggta tgtagtagga
     3721 cttaactgaa atgggtttta aaaaaataac tcctgaactg agtagttaaa aatactgtgt
     3781 agaaaagtat tctatattgc taggtttgtt cttttaacag ctgagattta ttaagatgca
     3841 ttattttgat tttaatcact gcctaaaaca ctttgggtgg tattgatgga gcggtggatt
     3901 ttcctccaag tggctaaatg caatttgaca tacttttcat ccaaagtttt gtacatcctg
     3961 ttgttttcta atggaaaaaa aaatgttaat atggcttttt tgtactacta aaaatagctt
     4021 tgagattaag gaaaataaat aactcttgta cagttgagaa tcgtgttatt aagtctgtat
     4081 tggcagtgtg tgcagtggcc tttgctgtgg ttatgaacac tctggattat aagaatcatt
     4141 tggtccaatt cctattgctt gtaaataaag ttttaccagt tgatat
//
