LOCUS       NM_001401668            5298 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus membrane associated ring-CH-type finger 8 (Marchf8),
            transcript variant 5, mRNA.
ACCESSION   NM_001401668 XM_006506639
VERSION     NM_001401668.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Chen,C., Wang,Y., Zhao,Q., Li,G.D., Wang,Y.H., Xu,L.C., Huang,H.Z.,
            Song,G., Li,W.T. and He,X.H.
  TITLE     E3 Ubiquitin Ligase MARCH8 Promotes Pancreatic Cancer Growth and
            Metastasis by Activating STAT3 via Degradation of PTPN4
  JOURNAL   Pancreas 52 (4), e224-e234 (2023)
   PUBMED   37747937
  REMARK    GeneRIF: E3 Ubiquitin Ligase MARCH8 Promotes Pancreatic Cancer
            Growth and Metastasis by Activating STAT3 via Degradation of PTPN4.
REFERENCE   3  (bases 1 to 5298)
  AUTHORS   Yang,X., Shi,C., Li,H., Shen,S., Su,C. and Yin,H.
  TITLE     MARCH8 attenuates cGAS-mediated innate immune responses through
            ubiquitylation
  JOURNAL   Sci Signal 15 (732), eabk3067 (2022)
   PUBMED   35503863
  REMARK    GeneRIF: MARCH8 attenuates cGAS-mediated innate immune responses
            through ubiquitylation.
REFERENCE   4  (bases 1 to 5298)
  AUTHORS   Liu,H., Wilson,K.R., Firth,A.M., Macri,C., Schriek,P., Blum,A.B.,
            Villar,J., Wormald,S., Shambrook,M., Xu,B., Lim,H.J.,
            McWilliam,H.E.G., Hill,A.F., Edgington-Mitchell,L.E., Caminschi,I.,
            Lahoud,M.H., Segura,E., Herold,M.J., Villadangos,J.A. and
            Mintern,J.D.
  TITLE     Ubiquitin-like protein 3 (UBL3) is required for MARCH
            ubiquitination of major histocompatibility complex class II and
            CD86
  JOURNAL   Nat Commun 13 (1), 1934 (2022)
   PUBMED   35411049
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5298)
  AUTHORS   Liu,X., Xu,F., Ren,L., Zhao,F., Huang,Y., Wei,L., Wang,Y., Wang,C.,
            Fan,Z., Mei,S., Song,J., Zhao,Z., Cen,S., Liang,C., Wang,J. and
            Guo,F.
  TITLE     MARCH8 inhibits influenza A virus infection by targeting viral M2
            protein for ubiquitination-dependent degradation in lysosomes
  JOURNAL   Nat Commun 12 (1), 4427 (2021)
   PUBMED   34285233
  REMARK    GeneRIF: MARCH8 inhibits influenza A virus infection by targeting
            viral M2 protein for ubiquitination-dependent degradation in
            lysosomes.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5298)
  AUTHORS   Jung,H., Park,H., Choi,Y., Kang,H., Lee,E., Kweon,H., Roh,J.D.,
            Ellegood,J., Choi,W., Kang,J., Rhim,I., Choi,S.Y., Bae,M.,
            Kim,S.G., Lee,J., Chung,C., Yoo,T., Park,H., Kim,Y., Ha,S.,
            Um,S.M., Mo,S., Kwon,Y., Mah,W., Bae,Y.C., Kim,H., Lerch,J.P.,
            Paik,S.B. and Kim,E.
  TITLE     Sexually dimorphic behavior, neuronal activity, and gene expression
            in Chd8-mutant mice
  JOURNAL   Nat Neurosci 21 (9), 1218-1228 (2018)
   PUBMED   30104731
REFERENCE   7  (bases 1 to 5298)
  AUTHORS   von Rohrscheidt,J., Petrozziello,E., Nedjic,J., Federle,C.,
            Krzyzak,L., Ploegh,H.L., Ishido,S., Steinkasserer,A. and Klein,L.
  TITLE     Thymic CD4 T cell selection requires attenuation of March8-mediated
            MHCII turnover in cortical epithelial cells through CD83
  JOURNAL   J Exp Med 213 (9), 1685-1694 (2016)
   PUBMED   27503071
  REMARK    GeneRIF: CD83-mediated MHCII stabilization through antagonism of
            March8 as a novel functional adaptation of cortical epithelial
            cells for T cell selection.
REFERENCE   8  (bases 1 to 5298)
  AUTHORS   Liu,H., Jain,R., Guan,J., Vuong,V., Ishido,S., La Gruta,N.L.,
            Gray,D.H., Villadangos,J.A. and Mintern,J.D.
  TITLE     Ubiquitin ligase MARCH 8 cooperates with CD83 to control surface
            MHC II expression in thymic epithelium and CD4 T cell selection
  JOURNAL   J Exp Med 213 (9), 1695-1703 (2016)
   PUBMED   27503069
  REMARK    GeneRIF: orchestrated regulation of MHC II surface expression in
            thymic epithelial cells by MARCH 8 and CD83 plays a major role in
            CD4(+) T cell selection.
REFERENCE   9  (bases 1 to 5298)
  AUTHORS   Toyomoto,M., Ishido,S., Miyasaka,N., Sugimoto,H. and Kohsaka,H.
  TITLE     Anti-arthritic effect of E3 ubiquitin ligase, c-MIR, expression in
            the joints
  JOURNAL   Int Immunol 23 (3), 177-183 (2011)
   PUBMED   21393633
REFERENCE   10 (bases 1 to 5298)
  AUTHORS   Ohmura-Hoshino,M., Matsuki,Y., Aoki,M., Goto,E., Mito,M.,
            Uematsu,M., Kakiuchi,T., Hotta,H. and Ishido,S.
  TITLE     Inhibition of MHC class II expression and immune responses by c-MIR
  JOURNAL   J Immunol 177 (1), 341-354 (2006)
   PUBMED   16785530
  REMARK    Erratum:[J Immunol. 2006 Sep 1;177(5):3493]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC123548.4.
            
            On Feb 17, 2022 this sequence version replaced XM_006506639.5.
            
            Summary: The protein encoded by this gene is a member of the
            membrane-associated really interesting new gene-CH family of
            proteins. These proteins are E3 ubiquitin-protein ligases that
            modulate antigen presentation by downregulating major
            histocompatibility complex class II surface expression through
            endocytosis. The transcript is primarily expressed by dendritic
            cells and macrophages. Overexpression of this gene in antigen
            presenting cells results in immune defective phenotypes, including
            resistance to autoimmune disease onset. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Oct
            2014].
            
            ##Evidence-Data-START##
            Transcript exon combination :: ERR2680377.171198.1,
                                           SRR7345562.4221401.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-194               AC123548.4         137222-137415       c
            195-299             AC123548.4         136947-137051       c
            300-470             AC123548.4         98574-98744         c
            471-521             AC123548.4         82863-82913         c
            522-607             AC123548.4         74315-74400         c
            608-1453            AC123548.4         73255-74100         c
            1454-1634           AC123548.4         71837-72017         c
            1635-1782           AC123548.4         69655-69802         c
            1783-5298           AC123548.4         65897-69412         c
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 53.78 cM"
     gene            1..5298
                     /gene="Marchf8"
                     /gene_synonym="1300017E09Rik; MARCH-VIII; March8; Mir"
                     /note="membrane associated ring-CH-type finger 8"
                     /db_xref="GeneID:71779"
                     /db_xref="MGI:MGI:1919029"
     exon            1..194
                     /gene="Marchf8"
                     /gene_synonym="1300017E09Rik; MARCH-VIII; March8; Mir"
                     /inference="alignment:Splign:2.1.0"
     exon            195..299
                     /gene="Marchf8"
                     /gene_synonym="1300017E09Rik; MARCH-VIII; March8; Mir"
                     /inference="alignment:Splign:2.1.0"
     exon            300..470
                     /gene="Marchf8"
                     /gene_synonym="1300017E09Rik; MARCH-VIII; March8; Mir"
                     /inference="alignment:Splign:2.1.0"
     CDS             378..2084
                     /gene="Marchf8"
                     /gene_synonym="1300017E09Rik; MARCH-VIII; March8; Mir"
                     /EC_number="2.3.2.27"
                     /note="isoform 3 is encoded by transcript variant 5;
                     c-mir, cellular modulator of immune recognition;
                     membrane-associated ring finger (C3HC4) 8; E3
                     ubiquitin-protein ligase MARCH8; membrane-associated
                     RING-CH protein VIII; membrane-associated RING finger
                     protein 8; RING-type E3 ubiquitin transferase MARCH8;
                     RING-type E3 ubiquitin transferase MARCHF8; E3
                     ubiquitin-protein ligase MARCHF8"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase MARCHF8 isoform 3"
                     /protein_id="NP_001388597.1"
                     /db_xref="GeneID:71779"
                     /db_xref="MGI:MGI:1919029"
                     /translation="MSMPLHQISAIPSQDAISARVYRSKTKDKEQNEKTLGHSMSHPS
                     NISKAGSSPPSTTAPVSAFSRTSVTPSNQDICSSSAVFSECCHFSPVQSAVVLKAPPC
                     QSSLTQGLTVTVLCKDTLQASKKNPFGSDWDQVLTPAKNTKTRRALRFSKSLSDVGEK
                     TQESLESFDYMERTCSEGKLVLPQGSSLKKNRLRHKEKRAEHCPPLSHSKHSPILSLS
                     TNHSAVSKREAGKGSVRIPLLEEKADGEARLRSQRLLRYLFSLSRGSSASSLHRFHEL
                     ESHASHLHTAKSSSWLAGSMDFCSDEMGDDDVFEDTSSAKLKNRVLRAPLCSVEKDSD
                     LDCPSLLSEKCTPISPVSTSGDACRICHCEGDDESPLITPCHCTGSLHFVHQACLQQW
                     IKSSDTRCCELCKYEFIMETKLKPLRKWEKLQMTASERRKIMCSVTFHVIAITCVVWS
                     LYVLIDRTAEEIKQGQVTGILEWPFWTKLVVVAIGFTGGLLFMYVQCKVYLQLWKRLK
                     AYNRVIYVQNCPETSKKNIFEKSALTEPTLENKEGHGMCHSTTNSSCTEPEDTGAEII
                     NV"
     exon            471..521
                     /gene="Marchf8"
                     /gene_synonym="1300017E09Rik; MARCH-VIII; March8; Mir"
                     /inference="alignment:Splign:2.1.0"
     exon            522..607
                     /gene="Marchf8"
                     /gene_synonym="1300017E09Rik; MARCH-VIII; March8; Mir"
                     /inference="alignment:Splign:2.1.0"
     exon            608..1453
                     /gene="Marchf8"
                     /gene_synonym="1300017E09Rik; MARCH-VIII; March8; Mir"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1634
                     /gene="Marchf8"
                     /gene_synonym="1300017E09Rik; MARCH-VIII; March8; Mir"
                     /inference="alignment:Splign:2.1.0"
     exon            1635..1782
                     /gene="Marchf8"
                     /gene_synonym="1300017E09Rik; MARCH-VIII; March8; Mir"
                     /inference="alignment:Splign:2.1.0"
     exon            1783..5298
                     /gene="Marchf8"
                     /gene_synonym="1300017E09Rik; MARCH-VIII; March8; Mir"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5274..5279
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Marchf8"
                     /gene_synonym="1300017E09Rik; MARCH-VIII; March8; Mir"
                     /note="hexamer: AATAAA"
     polyA_site      5298
                     /gene="Marchf8"
                     /gene_synonym="1300017E09Rik; MARCH-VIII; March8; Mir"
                     /note="major polyA site"
ORIGIN      
        1 agtcggcgtc ctctcgctgg gtggtgtagg ctgcgcgctg acccagcggc cctgctgcgt
       61 ccggcccggg tcatgcagga agcgggccgc gcgtggagct ccgcgcccac ctgagctgct
      121 cgcgccgagg tgcggcgtgg cggggccgcg cgctccgggc caccaggggt gtggccgctg
      181 ggaggccgcg acagtctccg cgtccgtccg agatggaagt gtggattcct ggtgtctgag
      241 tgcctgacgt ggcgtctgga cggtgctgct ctagtagaag cgctggaggc aaagcgtagc
      301 ttgaagtcaa tggcccaaaa tgactctgct agaggagact cacttccgta aggaggaaga
      361 ccaggtcgga ggctgggatg agcatgccat tgcaccagat ctctgccatc ccttctcagg
      421 atgccatttc tgctagagtc tacagaagca agaccaaaga taaggagcag aatgagaaga
      481 ctttgggaca ttccatgagt catccaagca acatttctaa ggctgggagt agtcctccat
      541 ccacgacggc tccagtgtct gccttctctc gcacttctgt cacaccatcc aaccaggaca
      601 tctgcagttc cagtgcagtg ttttctgagt gttgtcactt cagtcccgtg cagtctgctg
      661 ttgtcttgaa agctcctcca tgccagagtt ctctgacaca agggctcact gtgacagttc
      721 tatgtaaaga cacattacag gcatcaaaga aaaatccctt tggttcagac tgggaccaag
      781 tcttgacgcc cgctaaaaat actaagacca gaagagcact cagattctca aagtcactaa
      841 gtgatgtggg tgagaagacc caggagagtt tggaaagctt tgactatatg gaaagaactt
      901 gttctgaagg gaaattggtg cttcctcaag gttcatctct caaaaagaac aggctccgtc
      961 ataaagaaaa aagagcagag cactgcccac ccctcagcca ttccaaacac tctcctatct
     1021 tatccctttc taccaaccat tcagctgtct caaagaggga agctggcaag ggaagcgtgc
     1081 gcatcccact tttggaggag aaagctgatg gcgaggccag gttgagaagc cagcgactac
     1141 tccggtacct gttctcgctc tcccgtggct caagtgccag tagcctacat aggttccatg
     1201 agctggagag ccatgccagc catctgcaca ctgccaagtc ctccagctgg ctggcaggga
     1261 gcatggactt ctgttctgat gaaatgggag atgacgacgt ctttgaggat acatcatctg
     1321 ccaaattgaa gaacagggtc ctgcgtgcac ccctctgctc agtagagaag gacagtgacc
     1381 tggattgtcc ttctctgctc tctgaaaaat gtactcccat ctctcctgtg tccacttcag
     1441 gggatgcctg caggatctgc cactgtgaag gggatgacga gagccctctg atcaccccct
     1501 gtcactgcac agggagcctc catttcgtgc atcaggcttg cctgcagcag tggatcaaga
     1561 gttctgacac acgctgctgt gaactctgca agtacgagtt catcatggag accaagctga
     1621 aacctttgag gaaatgggag aagttgcaga tgactgccag tgagcgcagg aagatcatgt
     1681 gctcagtgac cttccatgtc attgctatca cctgtgtggt ctggtccttg tatgtgctca
     1741 ttgaccgcac agcagaggaa atcaagcagg gtcaggtaac aggaatccta gagtggcctt
     1801 tctggacgaa gctggtagtt gtggccatcg gcttcactgg aggacttctc tttatgtatg
     1861 ttcagtgcaa ggtgtaccta cagttatgga aaagactcaa ggcttacaat agagtgatct
     1921 atgttcagaa ctgtccagaa acaagtaaaa agaatatttt tgaaaagtct gcacttacag
     1981 agcccaccct tgaaaataaa gaaggacatg gaatgtgtca ttccaccaca aattcttctt
     2041 gcacagagcc tgaagacact ggagcagaaa ttattaacgt ctgaccatgt gagggtgtca
     2101 tttccttgat gtccaccaac agctgaagga atttgtctgc tgccagttgt gttccttctt
     2161 tctgtcgttt aatagcatag actgggcagg tgactattta tagtggcctc tctttttctc
     2221 aaccctcctt ggtgtgaatg tcctagaatt cttgtggggc aggtaccact gggttccatt
     2281 ctgccaggct tctctgtggt ctgggaaggt gggagacccc atgtatccat gacaaggagc
     2341 tggctaagtt ccagcccctg gtcttgaata gattatggaa gtaaaaagct aaagcagaag
     2401 acagagcagc atggggagag gaggcgggtg atggagggtc cccagaggac agagcagcat
     2461 ggtgcgggag ggctgctgct gctgggaggg gtccctcaca ctaacgagag ctaggagtgg
     2521 caagggagca gtgcctccag acagcagccc tgcagcccct gccttgctca gctgatgcca
     2581 ccaccttgct cggggggcag acaaggcctt gggctgtgtc tgctggtgtg tgactcactg
     2641 agaaaacagc actttatgag agtttttatt ttggaattcc tgtgtttaat gggagtgaca
     2701 tgtttaaaca cgtgtcacat ttagaaatgt tctctttatt gcctgttatc tctttgcatc
     2761 aaaatcgaac tgaaaccatt tctgccttat atattctgag tagtagatgt ccttccatgt
     2821 ccagaaaaga cttcagactt taagcaagac agacaggtct gagactccat gggaattaat
     2881 ggatgctaag agtgtaattc ctctggtttt gagcctgttt cctgaaaagt cctatctcac
     2941 aagctcaccc atacatttct ttgttgtgag cgttgaaatg aaagggtttt gagatgtaat
     3001 gaatctgaat cagggtgagc atttaaaaaa cgctcctttg actgacagat gtctccagat
     3061 ggagtgggta cacattgggg acactcctgt gatccacctc tgctgtcccc tgtgctgaca
     3121 aagttctgtg gagtcattgc tgagggctgg gaggtcaaca gaccagctct gggatgaggt
     3181 cttgggtgag cagtcagcta attgcatcgc cattgtgtca ctgacctggc ctcataccca
     3241 cttattgcct taagcagact tgacaggatc actagggtcc ttagctccaa tgtgacctat
     3301 tctgtcttgg ggttacctcc tgcgcgatat tttcacaaga cagacaaaat caaatcacta
     3361 ctgctaagtg gccattgccc ctttaaaaag tttgttgaac attttttttc ttgaaggcca
     3421 ttgtcaaagt gccaggcaat agtcaactta caatgtgaca agaggaaccg cagaagtgta
     3481 ctactctcca agtggctgct gtcggctcca gggtcttcag attctcttga ggtggccagg
     3541 gcaatgccta gggtccatct ccctgagctt tggctgcttc acctgtcttc tccagagact
     3601 gaggcaaacc gaagatgaat cttttgtagt tcctagtaga agccccgtgc ttagttttca
     3661 gaagcatgtg gaggctagct agaaggcttg tggaagcaag ttctgtcctg ttatcccctt
     3721 gtctactccc tggagtcatg gagttcagtt gtggagctgt tctgccaggg ctttcagcca
     3781 ttatctaatg ggcccacccc ttaggtcagc ctgccctctc tctcccagac cacataagcc
     3841 tccctgtgct cactgtgccc caaagtgcaa ttactcacac tcagtgagga caccaggcag
     3901 ctacagactg cccactaagc tctgactatt ccttttagca gctgctgcgg cgagtctctg
     3961 accctgtcca tctgtctgtg acaagatgtt ccaacattgg gtgggcagtc aaatgaagat
     4021 ggaggcattt gtagagaaga aagtgtccct caatgggggt tgtctctgca cagctgccct
     4081 aggggacttg tactcccaca tagataggtg gctttggaag catggcttca tgttgttccc
     4141 tttatttcag gaaggcctga tgctatctct tctctggctt ctctgatgaa ctctgtcctt
     4201 catccttccc ccgctcgttt tccctgaagg ttctctgatc tagtaaacaa ctacacatgt
     4261 gactcagtgc aggagactgg caaggcacgg ctgcgaagca tctggcagag ctctgggtct
     4321 gcccagactc tcctagtcct tgcctctttg gcctctgagt aattttgcag ggctatctgt
     4381 ctaccccatt gttcctcact ttgagcttag atatagtggg aagatggcat gtagctagac
     4441 caagcagatc taggatgagg gagacctcag gccaggaggg cttgtgcttg tgggtcaaat
     4501 gaagaaggaa gtcgtctcag ggtgtcactg gcagccctct aacttggcct ttaccccgta
     4561 tgtcatagac ccattcgcac agctttgact ctagctgcca caagtgagta gttgagggtt
     4621 atgctcaagt gtatcacaag attttcctgg aaatgtaaac attctcttag tctaggtctc
     4681 tctgtctctc ctttctggct tcaggacact cttcgttaag taggcttagt tttcaaacct
     4741 gtgggtatgt ccaagtggac acagatgtgc aggctcaggc ggtgggggat aggacagttc
     4801 aaggcctggg gtagtatcta agtgacagag gggaaaggct gtgtgggcag ggtgaagagc
     4861 tgtaatcttg tgggtgtggt tagatgcaca tgtatatatt tcattctacc tgttcaaatc
     4921 tggtttggaa taatgcagct tatagctgca gctcaaggaa agcccaaagc atagggagag
     4981 aaaaggcact tgtctctgtg tcgggagtga gtgacgaagg catgtaactg gtgcacattt
     5041 agatgcaacg tttcaagttg attgttaata aaaaccaaat ttacactggt gtgtgtagtg
     5101 tagtttctaa aaagcacttc acattttaaa tttttcttgg aaaatttcta gtaatctaaa
     5161 tgtctaattt tttaatcttt ttgtgtatgt tcattgtttc tcagtattac tgcttgaata
     5221 attctctgta caggggattt gtttgtgcta tatacggggt gtctaaagtt agcaataaag
     5281 cctttcttta caaaggaa
//
