LOCUS       NM_153058               4044 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus microtubule-associated protein, RP/EB family, member 2
            (Mapre2), transcript variant 1, mRNA.
ACCESSION   NM_153058
VERSION     NM_153058.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Yang,Y., Ma,D., Liu,B., Sun,X., Fu,W., Lv,F. and Qiu,C.
  TITLE     E3 Ubiquitin Ligase ASB14 Inhibits Cardiomyocyte Proliferation by
            Regulating MAPRE2 Ubiquitination
  JOURNAL   Cell Biochem Biophys 82 (2), 715-727 (2024)
   PUBMED   38319584
  REMARK    GeneRIF: E3 Ubiquitin Ligase ASB14 Inhibits Cardiomyocyte
            Proliferation by Regulating MAPRE2 Ubiquitination.
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Liu,H., Yue,J., Huang,H., Gou,X., Chen,S.Y., Zhao,Y. and Wu,X.
  TITLE     Regulation of Focal Adhesion Dynamics and Cell Motility by the EB2
            and Hax1 Protein Complex
  JOURNAL   J Biol Chem 290 (52), 30771-30782 (2015)
   PUBMED   26527684
  REMARK    GeneRIF: Knockdown of HAX1 and EB2 in skin epidermal cells
            stabilizes focal adhesions and impairs epidermal migration.
            Erratum:[J Biol Chem. 2019 Feb 22;294(8):2948. doi:
            10.1074/jbc.AAC119.007825.. PMID: 30796176]
REFERENCE   3  (bases 1 to 4044)
  AUTHORS   Yue,J., Xie,M., Gou,X., Lee,P., Schneider,M.D. and Wu,X.
  TITLE     Microtubules regulate focal adhesion dynamics through MAP4K4
  JOURNAL   Dev Cell 31 (5), 572-585 (2014)
   PUBMED   25490267
REFERENCE   4  (bases 1 to 4044)
  AUTHORS   Castro,D.S., Martynoga,B., Parras,C., Ramesh,V., Pacary,E.,
            Johnston,C., Drechsel,D., Lebel-Potter,M., Garcia,L.G., Hunt,C.,
            Dolle,D., Bithell,A., Ettwiller,L., Buckley,N. and Guillemot,F.
  TITLE     A novel function of the proneural factor Ascl1 in progenitor
            proliferation identified by genome-wide characterization of its
            targets
  JOURNAL   Genes Dev 25 (9), 930-945 (2011)
   PUBMED   21536733
REFERENCE   5  (bases 1 to 4044)
  AUTHORS   Jaworski,J., Kapitein,L.C., Gouveia,S.M., Dortland,B.R., Wulf,P.S.,
            Grigoriev,I., Camera,P., Spangler,S.A., Di Stefano,P., Demmers,J.,
            Krugers,H., Defilippi,P., Akhmanova,A. and Hoogenraad,C.C.
  TITLE     Dynamic microtubules regulate dendritic spine morphology and
            synaptic plasticity
  JOURNAL   Neuron 61 (1), 85-100 (2009)
   PUBMED   19146815
REFERENCE   6  (bases 1 to 4044)
  AUTHORS   Komarova,Y., Lansbergen,G., Galjart,N., Grosveld,F., Borisy,G.G.
            and Akhmanova,A.
  TITLE     EB1 and EB3 control CLIP dissociation from the ends of growing
            microtubules
  JOURNAL   Mol Biol Cell 16 (11), 5334-5345 (2005)
   PUBMED   16148041
REFERENCE   7  (bases 1 to 4044)
  AUTHORS   Jimenez-Mateos,E.M., Paglini,G., Gonzalez-Billault,C., Caceres,A.
            and Avila,J.
  TITLE     End binding protein-1 (EB1) complements microtubule-associated
            protein-1B during axonogenesis
  JOURNAL   J Neurosci Res 80 (3), 350-359 (2005)
   PUBMED   15789376
  REMARK    GeneRIF: Thus, we suggest that EB1 could complement MAP1B function
            during neural development.
REFERENCE   8  (bases 1 to 4044)
  AUTHORS   Zhou,F.Q., Zhou,J., Dedhar,S., Wu,Y.H. and Snider,W.D.
  TITLE     NGF-induced axon growth is mediated by localized inactivation of
            GSK-3beta and functions of the microtubule plus end binding protein
            APC
  JOURNAL   Neuron 42 (6), 897-912 (2004)
   PUBMED   15207235
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC132419.3 and AK148954.1.
            
            On Jul 9, 2009 this sequence version replaced NM_153058.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK046221.1, BC035254.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                AC132419.3         94866-94891         c
            27-4036             AK148954.1         2-4011
            4037-4044           AC132419.3         5000-5007           c
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 12.08 cM"
     gene            1..4044
                     /gene="Mapre2"
                     /gene_synonym="C820009F03Rik; D18Abb1e; EB1; EB2; RP1"
                     /note="microtubule-associated protein, RP/EB family,
                     member 2"
                     /db_xref="GeneID:212307"
                     /db_xref="MGI:MGI:106271"
     exon            1..252
                     /gene="Mapre2"
                     /gene_synonym="C820009F03Rik; D18Abb1e; EB1; EB2; RP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             134..1114
                     /gene="Mapre2"
                     /gene_synonym="C820009F03Rik; D18Abb1e; EB1; EB2; RP1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     end-binding protein 2; T-cell activation protein, EB1
                     family, member 2; APC-binding protein EB1; APC-binding
                     protein EB2"
                     /codon_start=1
                     /product="microtubule-associated protein RP/EB family
                     member 2 isoform 1"
                     /protein_id="NP_694698.3"
                     /db_xref="CCDS:CCDS29095.1"
                     /db_xref="GeneID:212307"
                     /db_xref="MGI:MGI:106271"
                     /translation="MPGPTQTLSPNGENNNDIIQDNGTIIPFRKHTVRGERSYSWGMA
                     VNVYSTSITQETMSRHDIIAWVNDIVSLNYTKVEQLCSGAAYCQFMDMLFPGCISLKK
                     VKFQAKLEHEYIHNFKLLQASFKRMNVDKVIPVEKLVKGRFQDNLDFIQWFKKFYDAN
                     YDGKEYDPVEARQGQDAIPPPDPGEQIFNLPKKSHHANSPTAGAAKSSPASKPGSTPS
                     RPSSAKRASSSGSASRSDKDLETQVIQLNEQVHSLKLALEGVEKERDFYFGKLREIEL
                     LCQEHGQENDDLVQRLMEVLYASDEQEGQTEEPEAEEQAHDQQPQQQEEY"
     exon            253..380
                     /gene="Mapre2"
                     /gene_synonym="C820009F03Rik; D18Abb1e; EB1; EB2; RP1"
                     /inference="alignment:Splign:2.1.0"
     exon            381..526
                     /gene="Mapre2"
                     /gene_synonym="C820009F03Rik; D18Abb1e; EB1; EB2; RP1"
                     /inference="alignment:Splign:2.1.0"
     exon            527..740
                     /gene="Mapre2"
                     /gene_synonym="C820009F03Rik; D18Abb1e; EB1; EB2; RP1"
                     /inference="alignment:Splign:2.1.0"
     exon            741..880
                     /gene="Mapre2"
                     /gene_synonym="C820009F03Rik; D18Abb1e; EB1; EB2; RP1"
                     /inference="alignment:Splign:2.1.0"
     exon            881..1039
                     /gene="Mapre2"
                     /gene_synonym="C820009F03Rik; D18Abb1e; EB1; EB2; RP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1040..4044
                     /gene="Mapre2"
                     /gene_synonym="C820009F03Rik; D18Abb1e; EB1; EB2; RP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2525..2530
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mapre2"
                     /gene_synonym="C820009F03Rik; D18Abb1e; EB1; EB2; RP1"
                     /note="hexamer: AATAAA"
     polyA_site      2552
                     /gene="Mapre2"
                     /gene_synonym="C820009F03Rik; D18Abb1e; EB1; EB2; RP1"
                     /note="major polyA site"
     regulatory      4018..4023
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mapre2"
                     /gene_synonym="C820009F03Rik; D18Abb1e; EB1; EB2; RP1"
                     /note="hexamer: AATAAA"
     polyA_site      4040
                     /gene="Mapre2"
                     /gene_synonym="C820009F03Rik; D18Abb1e; EB1; EB2; RP1"
                     /note="major polyA site"
ORIGIN      
        1 ggcctttgga gggcggggcc gggcacagag ctggagcaga gcgcggctgc agggtgagca
       61 gcaagcagcg gggagacaca accctccgcc ccagtcagcc accgcgtgtg ttccctctgt
      121 cgggagagcg ccaatgcccg ggccgaccca aacgttgtcc ccaaatggcg agaacaacaa
      181 cgacatcatc caggataacg ggactatcat tcctttccgg aagcacacag tgcgcgggga
      241 gcggtcctac agttggggaa tggcggtcaa tgtgtattct acctcgataa cccaggagac
      301 tatgagcagg catgacatca ttgcatgggt caatgacatc gtgtctctaa actacacaaa
      361 agtggaacag ctctgttcag gagcagccta ttgccagttc atggacatgc tcttcccggg
      421 ctgtattagt ttgaagaaag taaaattcca agcaaagttg gagcatgagt atattcacaa
      481 ctttaaactt ctgcaagcat cctttaaacg gatgaatgtt gataaggtaa tcccagtgga
      541 gaagctggtg aaagggcgtt tccaagacaa cctggatttt attcagtggt ttaagaagtt
      601 ctacgatgct aactatgacg ggaaggagta cgatcctgtt gaggcccgac aagggcaaga
      661 tgcaattcct ccccctgacc ctggtgaaca gatcttcaac ctgcccaaga agtcgcacca
      721 tgcaaactcc cccacagcag gcgcggctaa gtcaagtcct gcatctaagc caggatccac
      781 accttctcgc ccctcgtctg ccaaaagggc ttcatccagt ggctctgcat ccagatctga
      841 caaagattta gaaacgcagg tcatacagct caacgagcag gtacattcat tgaaactcgc
      901 cctagaaggt gtggagaagg agagggattt ctacttcggg aagttgagag agattgagct
      961 gctgtgccaa gaacatgggc aggagaacga tgacctcgtg cagcgactaa tggaagtgct
     1021 ctacgcttcc gatgaacagg agggccaaac ggaagagcca gaagcagagg agcaagccca
     1081 tgaccagcag ccccagcagc aggaggagta ctgaaccgtc tcatcagctc ttgacacttc
     1141 catcgtgcgt ggggactttg cttccagaga attggaacgt gtgtggcctg gagcgcagta
     1201 gaaaccagcc gttcccaacc tattcccatt gacagtgatc ttgcatccgc cagccattgc
     1261 tcttggctcc cacttcctct gccaagatgc gttagcagat ggccattctg gtcagctccc
     1321 tgccaggtca tagggtcaca taccgtcaac ttcacctcct ggctgcttaa gactagttct
     1381 actcccccct cataactatg tcccacccca acaccatcac caccaccacc accaccacca
     1441 ccccttttta tcctggtgtg aaacaatggt aatttgatat atggtattta tattggcatt
     1501 tttcaatcca gtgtcagtag ctgtcacgcg catttgtggt gctttgatgt ttgcgagtct
     1561 aacctcctct cataaatttg gtcagataat taattggaac aaagggaaac agatatttga
     1621 tatgaaagcc atatgacagt agcttgtgtc atggggaaac agtttctccc tctactcaca
     1681 acactaaagg gaaaaaggtg gattcagaac tatgacttca gggcccagca gtgttgatag
     1741 agcagcatat tagacaaaca cacagttgtt agttttcaga cattcgctca cggacaatac
     1801 catctcagct ttgcccttgc tccgtgtgtc cactcctgcc cccctccccc aggttttcct
     1861 gttattttct ttctcagggt cctttctttg gtgggggtcc actccccgaa agatgttgtc
     1921 attggcaatc tgcagttgca aagggggtag ggtggtctca gaaggcacga ggcttcctgc
     1981 ctcttccctc ttcctccttg taagagcttc aacccaagtc ctccaattca cattaaggaa
     2041 tccatactaa agggggactt gagggtgtgt accctacagg ccagccttcc acatcgtgtc
     2101 agtgttggct gcccaggttg gaagcgaaga cttgattgat agcatggttt caagagagaa
     2161 gtggggccac acacaaaatc accaatgatt ccgttacctt ctggagtaag gaccccaccc
     2221 tctctgcagc aagggggccc tccttgtcac tgacatttgc taaaccgtgg caattcatca
     2281 acgggaaaca ttagtgaatt gaaggcattc cttgttttta actaatattt gcacattttc
     2341 ttagtctcct tcctgatggt cgcccgtcct ccccttttga cggatggtat tctttcctaa
     2401 ctccctctgt tttgtttaat tttggcttta ggtttgctgt cactggtcat atggccagca
     2461 agtgcagaga aacaagaaac aaatgtgccc accccacctt ccacttaaag aaaaaaaatc
     2521 tcaaaataaa tttctgaatt atgcatcata aagctttgaa atttcttttt taattgatgg
     2581 gatactaacc ttaaattcta gcgttggtgc tttgcaccaa gagaaagctt tccaaaatta
     2641 accttcacag cagcgttgta agtaactgag gtccatgtgg aaaagatggc ttgtattttt
     2701 gaaatgatca caacacactg tgcagaagtg gacagatgtt ccatggcgtt tggttctcct
     2761 tccttctctc tcctgctcac ttctctgccg cgcaacacat ctctctgagg ctggcaagcc
     2821 tcgcccctcg cagtgacgtc ctcccacgcc tggcggccgt agtggctgtg ctccgggcag
     2881 catcgggctt cataggttcg ttgctctcct gagctttttt gattcacttg ctttgttttc
     2941 tgtttttttt aatagctatt ataaagaggt acaataagca gctattcaca gacacctggg
     3001 tggttcctat acctgcccag gacatcttaa taaccccagg gctggggact ccatggccac
     3061 actcctgtga atgggtatcg gggagttagg atctttcttc tcccaatgcc tcttgctgga
     3121 agcactctat acctctccac ctccctggac tactaagctg gagacctact ggccacatgt
     3181 ctgaaaaaat gttccctggc tagtgtactc cattctcttt cctagtggct cgggccagac
     3241 ttcatccatt tagagagaag cagtacttcc tgtttgtgtg cccatcttaa ataccttgaa
     3301 aagtactatg tcccaggtgg tgtcatggat gctttgggga atccttcgca gctatttcca
     3361 cccctgcctc tgggcaccca ccactcacgg atggcagaac ccattcagtc tcctaccttg
     3421 cattcaggat gcacagttct ctaagtcctg gccagcccca gctcatcaca ttgggtccac
     3481 aggcatccca gcatgggtcc ctgcgcctcc ctctcaagga gttgattttt tttcccctca
     3541 gtttccccac acccactgtt ggctggaagt gcttccatta ccactgtcag attggaagtg
     3601 accgaagcag gggatgaaga gaaagctcac attctaagta gagaagcagg aaaaattgtt
     3661 caaaagacaa acaccctgtt gaactgagag aagtaaactc cagaagggaa ccaagatttc
     3721 ctccctttcc tggtgagtgt ttccattatt ccgttaaggt ttaatatgca ttcagattac
     3781 ttttactgaa tagtacacca taaagctttt gttatatatt aaatgtaaac tgaaaggaat
     3841 gtaaacatat gtattgttaa ttataaatat cgataagtaa tagcataata gatgaagagg
     3901 tcttattcag atgcttcatg tccattttac attacccatc tactgtcgca tggtaggata
     3961 gctttttgtc tctgaatatg tgaataactt gacttgcgtt gatcttttta catatttaat
     4021 aaaaaaacta tacgttaaaa tctg
//
