LOCUS       NM_177345               3452 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus mitogen-activated protein kinase associated protein 1
            (Mapkap1), transcript variant 1, mRNA.
ACCESSION   NM_177345 NM_133841 XM_006497974
VERSION     NM_177345.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Lin,X., Cheng,L., Wan,Y., Yan,Y., Zhang,Z., Li,X., Wu,J., Wang,X.
            and Xu,M.
  TITLE     Ang II Controls the Expression of Mapkap1 by miR-375 and Affects
            the Function of Islet beta Cells
  JOURNAL   Endocr Metab Immune Disord Drug Targets 23 (9), 1186-1200 (2023)
   PUBMED   36748222
  REMARK    GeneRIF: Ang II Controls the Expression of Mapkap1 by miR-375 and
            Affects the Function of Islet beta Cells.
REFERENCE   3  (bases 1 to 3452)
  AUTHORS   Liu,H., Zhang,D., Zhou,Y. and Cui,S.
  TITLE     MicroRNA-7a inhibits Isl1 expression to regulate insulin secretion
            by targeting Raf1 and Mapkap1 in NIT-1 cells
  JOURNAL   In Vitro Cell Dev Biol Anim 57 (8), 817-824 (2021)
   PUBMED   34713362
  REMARK    GeneRIF: MicroRNA-7a inhibits Isl1 expression to regulate insulin
            secretion by targeting Raf1 and Mapkap1 in NIT-1 cells.
REFERENCE   4  (bases 1 to 3452)
  AUTHORS   Baffi,T.R., Lorden,G., Wozniak,J.M., Feichtner,A., Yeung,W.,
            Kornev,A.P., King,C.C., Del Rio,J.C., Limaye,A.J., Bogomolovas,J.,
            Gould,C.M., Chen,J., Kennedy,E.J., Kannan,N., Gonzalez,D.J.,
            Stefan,E., Taylor,S.S. and Newton,A.C.
  TITLE     mTORC2 controls the activity of PKC and Akt by phosphorylating a
            conserved TOR interaction motif
  JOURNAL   Sci Signal 14 (678) (2021)
   PUBMED   33850054
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3452)
  AUTHORS   Li,M., Lazorchak,A.S., Ouyang,X., Zhang,H., Liu,H., Arojo,O.A.,
            Yan,L., Jin,J., Han,Y., Qu,G., Fu,Y., Xu,X., Liu,X., Zhang,W.,
            Yang,Z., Ruan,C., Wang,Q., Liu,D., Huang,C., Lu,L., Jiang,S., Li,F.
            and Su,B.
  TITLE     Sin1/mTORC2 regulate B cell growth and metabolism by activating
            mTORC1 and Myc
  JOURNAL   Cell Mol Immunol 16 (9), 757-769 (2019)
   PUBMED   30705387
  REMARK    GeneRIF: Sin1/mTORC2 regulate B cell growth and metabolism by
            activating mTORC1 and Myc.
REFERENCE   6  (bases 1 to 3452)
  AUTHORS   Jacinto,E., Facchinetti,V., Liu,D., Soto,N., Wei,S., Jung,S.Y.,
            Huang,Q., Qin,J. and Su,B.
  TITLE     SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt
            phosphorylation and substrate specificity
  JOURNAL   Cell 127 (1), 125-137 (2006)
   PUBMED   16962653
  REMARK    GeneRIF: Results reveal that the SIN1-rictor-mTOR function in
            Akt-Ser473 phosphorylation is required for TORC2 function in cell
            survival but is dispensable for TORC1 function.
REFERENCE   7  (bases 1 to 3452)
  AUTHORS   Michel,J.J., Townley,I.K., Dodge-Kafka,K.L., Zhang,F.,
            Kapiloff,M.S. and Scott,J.D.
  TITLE     Spatial restriction of PDK1 activation cascades by anchoring to
            mAKAPalpha
  JOURNAL   Mol Cell 20 (5), 661-672 (2005)
   PUBMED   16337591
REFERENCE   8  (bases 1 to 3452)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   9  (bases 1 to 3452)
  AUTHORS   Sarbassov,D.D., Ali,S.M., Kim,D.H., Guertin,D.A., Latek,R.R.,
            Erdjument-Bromage,H., Tempst,P. and Sabatini,D.M.
  TITLE     Rictor, a novel binding partner of mTOR, defines a
            rapamycin-insensitive and raptor-independent pathway that regulates
            the cytoskeleton
  JOURNAL   Curr Biol 14 (14), 1296-1302 (2004)
   PUBMED   15268862
REFERENCE   10 (bases 1 to 3452)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB852269.1, AK042154.1, AK052045.1, BC090644.1 and AL845262.3.
            
            On or before Mar 15, 2014 this sequence version replaced
            XM_006497974.1, NM_177345.3.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a). Variants 1, 2, and 4 all encode the same isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC096618.1, AK052045.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-215               BB852269.1         2-216
            216-332             AK042154.1         206-322
            333-2407            AK052045.1         1-2075
            2408-3444           BC090644.1         1982-3018
            3445-3452           AL845262.3         122149-122156
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 22.84 cM"
     gene            1..3452
                     /gene="Mapkap1"
                     /gene_synonym="D230039K05Rik; Sin1"
                     /note="mitogen-activated protein kinase associated protein
                     1"
                     /db_xref="GeneID:227743"
                     /db_xref="MGI:MGI:2444554"
     exon            1..404
                     /gene="Mapkap1"
                     /gene_synonym="D230039K05Rik; Sin1"
                     /inference="alignment:Splign:2.1.0"
     exon            405..732
                     /gene="Mapkap1"
                     /gene_synonym="D230039K05Rik; Sin1"
                     /inference="alignment:Splign:2.1.0"
     CDS             474..2042
                     /gene="Mapkap1"
                     /gene_synonym="D230039K05Rik; Sin1"
                     /note="isoform a is encoded by transcript variant 1;
                     target of rapamycin complex 2 subunit MAPKAP1; TORC2
                     subunit MAPKAP1; SAPK-interacting protein 1;
                     stress-activated map kinase-interacting protein 1;
                     mitogen-activated protein kinase 2-associated protein 1"
                     /codon_start=1
                     /product="target of rapamycin complex 2 subunit MAPKAP1
                     isoform a"
                     /protein_id="NP_796319.1"
                     /db_xref="CCDS:CCDS15948.1"
                     /db_xref="GeneID:227743"
                     /db_xref="MGI:MGI:2444554"
                     /translation="MAFLDNPTIILAHIRQSHVTSDDTGMCEMVLIDHDVDLEKTHPP
                     SVPGDSGSEVQGSSGETQGYIYAQSVDITSSWDFGIRRRSNTAQRLERLRKERQNQIK
                     CKNIQWKERNSKQSAQELKSLFEKKSLKEKPPSSGKQSILSVRLEQCPLQLNNPFNEY
                     SKFDGKGHVGTTATKKIDVYLPLHSSQDRLLPMTVVTMASARVQDLIGLICWQYTSEG
                     REPKLNDNVSAYCLHIAEDDGEVDTDFPPLDSNEPIHKFGFSTLALVEKYSSPGLTSK
                     ESLFVRINAAHGFSLIQVDNTKVTMKEILLKAVKRRKGSQKISGPQYRLEKQSEPNIA
                     VDLESTLESQNAWEFCLVRENSSRADGVFEEDSQIDIATVQDMLSSHHYKSFKVSMIH
                     RLRFTTDVQLGISGDKVEIDPVTNQKASTKFWIKQKPISIDCDLLCACDLAEEKSPSH
                     AVFKLTYLSSHDYKHLYFESDAATVSEIVLKVNYILESRASTARADYLAQKQRKLNRR
                     TSFSFQKEKKSGQQ"
     exon            733..822
                     /gene="Mapkap1"
                     /gene_synonym="D230039K05Rik; Sin1"
                     /inference="alignment:Splign:2.1.0"
     exon            823..971
                     /gene="Mapkap1"
                     /gene_synonym="D230039K05Rik; Sin1"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1144
                     /gene="Mapkap1"
                     /gene_synonym="D230039K05Rik; Sin1"
                     /inference="alignment:Splign:2.1.0"
     exon            1145..1321
                     /gene="Mapkap1"
                     /gene_synonym="D230039K05Rik; Sin1"
                     /inference="alignment:Splign:2.1.0"
     exon            1322..1431
                     /gene="Mapkap1"
                     /gene_synonym="D230039K05Rik; Sin1"
                     /inference="alignment:Splign:2.1.0"
     exon            1432..1539
                     /gene="Mapkap1"
                     /gene_synonym="D230039K05Rik; Sin1"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1680
                     /gene="Mapkap1"
                     /gene_synonym="D230039K05Rik; Sin1"
                     /inference="alignment:Splign:2.1.0"
     exon            1681..1818
                     /gene="Mapkap1"
                     /gene_synonym="D230039K05Rik; Sin1"
                     /inference="alignment:Splign:2.1.0"
     exon            1819..1916
                     /gene="Mapkap1"
                     /gene_synonym="D230039K05Rik; Sin1"
                     /inference="alignment:Splign:2.1.0"
     exon            1917..3452
                     /gene="Mapkap1"
                     /gene_synonym="D230039K05Rik; Sin1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2389..2394
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mapkap1"
                     /gene_synonym="D230039K05Rik; Sin1"
                     /note="hexamer: AATAAA"
     polyA_site      2409
                     /gene="Mapkap1"
                     /gene_synonym="D230039K05Rik; Sin1"
                     /note="major polyA site"
     regulatory      3425..3430
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mapkap1"
                     /gene_synonym="D230039K05Rik; Sin1"
                     /note="hexamer: AATAAA"
     polyA_site      3444
                     /gene="Mapkap1"
                     /gene_synonym="D230039K05Rik; Sin1"
                     /note="major polyA site"
ORIGIN      
        1 acgcggggta cggttccggg tcgtggcgcg gctctgggta acagggctgc tgttgccggc
       61 cggcggcggc ggggtcatga gggcgtgccc ggggcgccag ggccgagtgg gccggtagga
      121 ctgccgagga cttcgtaggc gctgggttcg gtgcaggggc cgggaggcgt gggggagtcg
      181 ggacctccct cagggacgtg tcccgggcga ggcggcctgt agtgtgggag cagcttcagg
      241 taaaggttgc cccagccgtc gccccctcgc ccgcgggacc ctccgacctc tcagcacccc
      301 gctgccgtcg ctgcccagcg gcgcccgttg gggctgtgct cggtggcctg ggaacatcgg
      361 tttccttgct cttcctgagc ctgacagctg ggtgtgtgac ttaggtaggt gagcctgtga
      421 aacagtatga agagaagaaa atagcctttt aaagagactg gcccacagaa aggatggcct
      481 tcttggacaa tccaactatc attctagctc atattcgaca gtcacacgtg accagtgatg
      541 acacaggaat gtgtgagatg gttctcattg atcatgacgt tgacctagag aagactcatc
      601 ctccgtcagt gcctggagac agtgggtcag aagttcaggg aagcagtggt gagacgcagg
      661 gctacatata cgcccagtct gttgatatta catcgagctg ggactttggt attagaagac
      721 gctcaaacac agctcaaaga ttagaacgac tccgcaaaga gagacaaaac cagatcaaat
      781 gcaaaaatat tcagtggaaa gaaagaaatt ctaaacaatc agcccaggag ttaaagtcac
      841 tgtttgaaaa aaaatccctc aaagagaagc ctccaagttc aggcaagcag tccatattgt
      901 ctgtacgcct ggagcagtgc cctctgcagc tgaataaccc ctttaatgag tactccaagt
      961 ttgatggcaa gggtcatgtg ggtacaactg caactaagaa gatcgacgtc tacctccccc
     1021 tgcactccag ccaggacaga ctgctgccaa tgaccgtggt gaccatggcc agcgccaggg
     1081 tgcaggacct catcgggctc atctgttggc agtacacgag tgaaggacgg gagccgaagc
     1141 tcaatgacaa tgtcagtgcc tactgcctgc atattgctga ggatgatggg gaggtcgaca
     1201 cggattttcc gccactggat tccaatgagc ccattcataa gtttggcttc agtactttgg
     1261 ccctggttga aaaatactcc tctcctggtc tgacctccaa agagtcgctc tttgttcgaa
     1321 taaatgctgc tcatggattc tcccttatcc aagtagacaa cacaaaggtc accatgaagg
     1381 agatcttgct caaggcagtg aaaagaagaa aaggatccca gaaaatttca ggccctcagt
     1441 accgcctgga gaagcagagt gagcctaaca tcgctgtgga cctggagagc acgctggaga
     1501 gccagaacgc ctgggagttc tgcctggttc gcgagaacag ttcaagggca gacggagttt
     1561 tcgaggagga ttcacaaatt gacatcgcta cagtacagga tatgcttagc agccaccatt
     1621 acaagtcatt caaagtcagc atgatccaca gactgcgatt cacgacggat gtgcagttag
     1681 gtatctctgg agacaaagta gagatagacc ctgttacgaa tcagaaagcc agcactaagt
     1741 tttggattaa gcagaaaccc atctcaatcg attgtgacct gctctgtgcc tgtgaccttg
     1801 ccgaggagaa gagccccagt cacgcggtgt ttaagctcac gtatctaagc agccatgact
     1861 acaagcacct gtacttcgag tccgacgcag ccacagtcag tgagatcgtg ctcaaggtta
     1921 actacatcct ggaatcacga gcgagcactg cccgagctga ttatcttgct caaaaacaaa
     1981 gaaaactgaa caggcgaaca agcttcagct tccagaagga gaagaaatca gggcagcagt
     2041 gacactggcc tctagtctgg tgccacagcc caggtggcct gcaaggccag gtggaccgga
     2101 gtccaccagc atcctgtagt gccgagaccc gcaggcacag gctgctgggc tctaggggaa
     2161 ggtttagagg taggggtgcg attggactga aacttggcaa tggttctaag tagcaatgcc
     2221 caggagccag gccacctcct ggaggggctg agtgctgctc agccgtgtgt cctgcctggc
     2281 agtcccagca agactgtaca tagccatgcc agacatctcc ttgcaacttg atcaaatttc
     2341 ttaaagcaaa caaagaacaa aagtgtacat ttctctttct ttccttttaa taaacaggta
     2401 tactctttat cgtggttggt atgatggacc attctttggg gtagaggatt gactatatta
     2461 tcttccttaa aatctgttcc catattgaac aggcagatta caaaagctgt ggttggagct
     2521 ctcagaagca atgtttaggg cttagccagt agtttaactg tgccatttgt ttaaataagt
     2581 gatttgcttt gagaatagca tcttactaaa gttaggaatt gaggacctag catgtgccca
     2641 gcgtaagtgc atgcgtgggt gcgtgcgtgc gtgcgtgcgt gcgtgcgtag ccttctaagg
     2701 ccttctcaac atgtcagcgc ctgtgatctg agagacaggt gctggtaata tagtgtgaca
     2761 gtgtctccaa gtggtgctga gttcccagcc agggtcttct ctcatctcct catagcaagt
     2821 atgatatggt cagcctgcct ctctgaccta tctccaatcc tcttaccggt gcctgctgtg
     2881 gagccagacg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     2941 gctgctgctg ctgctgctgc tgctgctata ctgaatcagg gagaaggaga gcaagccctg
     3001 ggcagaaaag gcaaggtacc cagcaccctg acaccacacc acacctctgt ctacaccaga
     3061 tgcctctgca gacctcacca tggtcccgcc tgtggcagca tccttgggcc agggccacag
     3121 ccgatatctt atacgacctg tgtttaggac attttttaaa tcattgtgtc taagcatctc
     3181 agagtcaagt gtgcttcttt gcaaaggggt cgactgaatg aatgcctctc tcattctgat
     3241 ctgttcagcc aacaggagca cccttttcta atgtcttcca ttcctatctc cccccaaaag
     3301 aaatattatt tgtagcttgc tctgtatttg aatttttagc aattttatat ttagatatct
     3361 ttgaaattgt aaatgactaa tttggtcatt aaatcttgtc acatattcaa tattaaaata
     3421 ctaaaataaa agtcataaat accctctttt gt
//
