LOCUS       NM_201519               4490 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus mitogen-activated protein kinase kinase kinase kinase
            5 (Map4k5), mRNA.
ACCESSION   NM_201519 NM_024275
VERSION     NM_201519.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   3  (bases 1 to 4490)
  AUTHORS   Shi,C.S., Huang,N.N., Harrison,K., Han,S.B. and Kehrl,J.H.
  TITLE     The mitogen-activated protein kinase kinase kinase kinase GCKR
            positively regulates canonical and noncanonical Wnt signaling in B
            lymphocytes
  JOURNAL   Mol Cell Biol 26 (17), 6511-6521 (2006)
   PUBMED   16914735
  REMARK    GeneRIF: Germinal center kinase-related enzyme (GCKR) facilitates
            both canonical and noncanonical Wnt signaling in B lymphocytes.
REFERENCE   4  (bases 1 to 4490)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK053775.1, AK032123.1, AK019468.1, AK084891.1 and BC048173.1.
            
            On or before Dec 21, 2007 this sequence version replaced
            NM_024275.2, NM_201519.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK053775.1, AK084891.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-113               AK053775.1         2-114
            114-484             AK032123.1         1-371
            485-556             AK019468.1         332-403
            557-4089            AK032123.1         443-3975
            4090-4251           AK084891.1         3798-3959
            4252-4490           BC048173.1         4134-4372
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 28.94 cM"
     gene            1..4490
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /note="mitogen-activated protein kinase kinase kinase
                     kinase 5"
                     /db_xref="GeneID:399510"
                     /db_xref="MGI:MGI:1925503"
     exon            1..511
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     CDS             404..2947
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /EC_number="2.7.11.1"
                     /note="MEKKK 5; MEK kinase kinase 5; MAPK/ERK kinase
                     kinase kinase 5"
                     /codon_start=1
                     /product="mitogen-activated protein kinase kinase kinase
                     kinase 5"
                     /protein_id="NP_958927.2"
                     /db_xref="GeneID:399510"
                     /db_xref="MGI:MGI:1925503"
                     /translation="MEAPLRPAADILRRNPQHDYELVQRVGSGTYGDVYKARNVHTGE
                     LAAVKIIKLEPGDDFSLIQQEIFMVKECKHCNIVAYFGSYLSREKLWICMEYCGGGSL
                     QDIYHVTGPLSEMQIAYVCRETLQGLAYLHTKGKMHRDIKGANILLTDHGDVKLADFG
                     VAAKITATIAKRKSFIGTPYWMAPEVAAVEKNGGYNQLCDIWAVGITAIELGELQPPM
                     FDLHPMRALFLMSKSNFQPPKLKDKTKWSSTFHNFVKIALTKNPKKRPTAERLLTHTF
                     VGQPGLSRALAVELLDKVSNPDNHAPYSEGDEDDLEPHAIIRHTIRSTNRNSRAERTA
                     SEINFDKLQFEPPLRKETEARDEMGLSSEPNFILHWNPFVDGANTGRSTSKRAIPPPL
                     PPKPRVNTYPEDSLPDEEKSSTIKRCPDLEARAPQVLRRQSSPSCVPVAETSSSIGNG
                     DGISKLISENTEGSAQAPQLPRKKDKRDFPKPTINGLPPTPKVLMGACFSKVFDGCPL
                     KINCATSWIHPDTKDQYIIFGTEDGIYTLNLNELHEATMEQLFPRKCTWLYVINNTLM
                     SLSEGKTFQLYSHNLIALFEQAKKPGLAAHIQTHRFPDRILPRKFALTTKIPDTKGCH
                     KCCIVRNPYTGHKYLCGALQSGIVLLQWYEPMQKFMLIKHFDFPLPSPLNVFEMLVIP
                     EQEYPMVCVAISKGSDSSQVVQFETINLNSASSWFTEIGAGSQQLDSIHVTQLERDTV
                     LVCLDKFVKIVNLQGKLKSSKKLASELSFDFRIESVVCLQDSVLAFWKHGMQGKSFKS
                     DEVTQEISDETRVFRLLGSDRVVVLESRPTENPAAHSNLYILAGHENSY"
     exon            512..569
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            570..660
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            661..725
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            726..781
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            782..829
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            830..872
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            873..945
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1077
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1078..1140
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1141..1222
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1339
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1418
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1477
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1478..1545
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1546..1588
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1745
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1746..1843
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1844..1885
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..1970
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            1971..2050
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            2051..2111
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            2112..2114
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            2115..2229
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            2230..2288
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            2289..2392
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            2393..2570
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            2571..2639
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            2640..2732
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            2733..2803
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            2804..2859
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     exon            2860..4454
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4433..4438
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /note="hexamer: AATAAA"
     polyA_site      4454
                     /gene="Map4k5"
                     /gene_synonym="4432415E19Rik; GCKR; KHS; MAPKKKK5"
                     /note="major polyA site"
ORIGIN      
        1 actcggcggg cctcagggcg gcagtctggg cccgcggcct gcgttctcgg gcgggccagg
       61 ctcgatccgc gccggccgcg aacgtgtctc ccggtgacgc agccgcgggt ggggaacgtg
      121 gtgcggcggc gactgtgcgc acggccgtct cggagaagac gcgggtctcc gacgccggcg
      181 ctcggccaag aacggacggc ctctcgggta cggccggcgc ggggcctggc gggaccgacg
      241 aggcccggag gctgccgggg ttgcgccccg tgacgccccc cgcgctcgct ctcgccggtg
      301 caggcgcagc tgcgagccga ggctccatgt tgggaagcgc cgcgcgccct cgttagcggg
      361 agacggctgc ggcgggcaga gccccggact gggcgcagcg aagatggagg ccccgctgag
      421 gcctgccgcc gacatcctga gacggaaccc gcagcacgac tacgagctgg tgcagagggt
      481 cggcagcggc acctacgggg acgtgtacaa ggccagaaat gtacatacag gagaattagc
      541 tgcagtaaag atcatcaagt tggagcctgg tgatgatttt tctttgattc agcaagagat
      601 atttatggtt aaagaatgta agcattgcaa catcgtagcc tactttggga gctatctcag
      661 tagggagaag ctgtggattt gcatggagta ctgcggtggc ggatcacttc aagatatcta
      721 tcacgtgact gggccattat ctgaaatgca aatagcctac gtctgcagag agaccttaca
      781 gggtcttgcc tacttgcata ctaaaggcaa aatgcataga gatatcaaag gtgcaaatat
      841 cttattaaca gaccatggtg atgtaaaatt agctgatttt ggtgtggctg caaaaattac
      901 agctaccatt gcaaagagga agtccttcat cggcacccct tactggatgg ctccagaagt
      961 tgcagcagtg gaaaagaatg gtggctacaa ccaactctgt gatatctggg ccgtcggaat
     1021 aacagcaatc gagctgggag aactgcagcc gcctatgttt gacctgcatc caatgagggc
     1081 tctcttctta atgtcaaaaa gtaatttcca gcctccaaag ctaaaggaca aaactaaatg
     1141 gtcatcgaca ttccataatt ttgtcaaaat agcactaact aaaaacccca agaaaagacc
     1201 aactgctgaa aggcttctga ctcacacttt tgttgggcag ccaggcctgt ctcgggcgct
     1261 cgcggttgag ctgttggaca aggtgagcaa tccggacaac catgctccct acagtgaggg
     1321 cgatgaggac gacttggagc cccacgcaat cattcgtcat accattagat caacaaacag
     1381 gaattccaga gcggagcgga cagcttcaga aataaacttt gacaaattac aatttgaacc
     1441 tcctctgaga aaagaaacag aagcccggga tgaaatggga ttatcatcag agccaaactt
     1501 tatattacat tggaatcctt ttgtggatgg agcaaataca ggcagatcaa cctctaagcg
     1561 tgcaatacca cctccgctac ctcctaagcc gagggtcaac acttaccctg aagacagcct
     1621 cccagatgaa gaaaaatcat caaccatcaa acgttgccct gatctggagg cgagagcacc
     1681 tcaggtcctc agaagacaga gtagcccaag ttgtgttcct gtggcggaga catcctcctc
     1741 tataggaaat ggtgatggta tttcaaaact gatcagtgaa aatacagagg gatccgcaca
     1801 agctccgcag ttacctcgga aaaaggacaa gcgggacttc cccaaaccaa ccatcaatgg
     1861 ccttccaccc accccaaaag tactgatggg agcatgtttt tcaaaagtat ttgatggctg
     1921 ccctttgaaa attaattgtg caacatcctg gatacaccct gatacaaaag atcagtatat
     1981 tatttttgga actgaagatg gtatttacac attgaatctc aatgagctac atgaagcaac
     2041 tatggaacag ttatttccac ggaaatgtac gtggctctac gtcatcaata atacactgat
     2101 gtcattatca gaaggaaaaa cctttcagct ctactctcac aaccttattg ctttgtttga
     2161 acaagccaaa aaaccagggt tagccgccca catccaaact cacaggtttc ctgaccgcat
     2221 tctgccaaga aaatttgcct taacaactaa gattcccgat acaaaaggct gccacaaatg
     2281 ctgcatagtt agaaaccctt acacgggaca taagtacctc tgtggagctt tgcagtctgg
     2341 catcgttctg cttcagtggt atgagccgat gcagaaattc atgctgataa agcatttcga
     2401 ttttccttta ccaagtccac tgaatgtttt tgaaatgctg gtgatcccag agcaggagta
     2461 ccccatggtt tgtgtggcta tcagcaaagg ctcggactcc agccaagtag ttcagtttga
     2521 gacaatcaac ttgaattctg catcttcctg gtttacagaa attggtgcag gcagccaaca
     2581 gctggattcc attcatgtga cacagttgga gagagatact gttttggtgt gtttagacaa
     2641 atttgtaaaa attgtaaatc tacaaggaaa gctgaagtcc agtaagaagc tggcatctga
     2701 gctcagtttt gacttccgca ttgagtctgt ggtgtgcctt caagacagcg tgttggcttt
     2761 ctggaaacac gggatgcagg gtaaaagctt caaatcagat gaggttaccc aggaaatttc
     2821 agatgagacc agggttttcc gcctgctggg atcagacagg gtggtggttt tggaaagccg
     2881 gccaacagaa aaccctgcag cacacagcaa tctctacatc ctggctggac atgaaaacag
     2941 ttactaagca gcagaggcga attccaagtg acaagaggat gaatgaacat gttccattga
     3001 aaacaagagt gtgttaaggg aattcagtgc cgactgcagt gtaacttgct ttaattattg
     3061 tgggttttat atcaaatgaa ctgtacatta ggatagaatt cagtattttc tgtagctgga
     3121 aacagctagt caatctcttg ccactgtgtg gtggttatat caagtttgct taataaaagc
     3181 tatgagacaa atagtcctct agttccaaga aacacagtcc tttttaaaaa acaatgtttg
     3241 taacaaggtg ccatggtgtt tttaaataac tcatgattct ctggagaggg agtttactga
     3301 gcattatatc attttatacc atgtcttact ccttcttagt gaacataatt tgataagacg
     3361 ttatcgaaca agcctttcac tttgacattg gccgttctgt atgtttttgt aagatagaat
     3421 atttaatcct tatttattaa tctcttgctg gagtggtgta atgtttctaa cttttagcaa
     3481 aggaaggttg cagagcagct gaaatttttt ataatgtgtg aaaatgttgt ttaccttctc
     3541 agagtagtgc tttgggggtt cctggcggcg tgttggaggt ctagcacata cagcttccct
     3601 taacagaata ttttacgaca gtttcataca gggttgcctt aacgggaatt ggtttccaac
     3661 tccagatggt tgtaagggct cgtgcagaaa tactgatggt ggacacaaag cctcagcagg
     3721 gacgtgtagt gtccaggtgt gactaccgga gttttgacaa gcagtataat atacctgtga
     3781 ttttgttttt tacattaggg ataatgcata agaaattaat ctttatatat atcatgaccc
     3841 ctaatgtttg ggaaaaaagt atttaactgc ccatattatg tattttactt atgttatgcc
     3901 aagggagaaa gtaattacac gtgtaatctt ggggttttca catacataga tattcattgt
     3961 gagtaggctt aaggaaaaaa aaaactttaa gtgaaattgg aatttctgat gggatagtat
     4021 agataaatga tgttgtttta tattttttaa aaaaccagta tactaaaatt aaaaaaggat
     4081 agggttgtct tttagtttgt ttttatttta caattcttag tattcagaat tgatgtgttt
     4141 tattggtacc agctgtctta atgcatttat taagaacagt tagcattgag gtaatgaaca
     4201 caggggctaa gagtagcaaa cgatagacgt agtctgaccg gatacttgag gaaaacaatg
     4261 tacctatctc atctttacag ttttataaag tccatgttat catctccacc ttccaaatga
     4321 ggaggtaaga gtttaaccaa gttaaacaac ttacacgtta catactggtt agttctttga
     4381 gccagaccaa gagtcctccg actctgctct tttcactatg caatatacaa gtaataaaat
     4441 gttacgcaaa tgaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//
