LOCUS       NM_001252200            5782 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus mitogen-activated protein kinase kinase kinase kinase
            4 (Map4k4), transcript variant 1, mRNA.
ACCESSION   NM_001252200
VERSION     NM_001252200.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Juin,A., Spence,H.J. and Machesky,L.M.
  TITLE     Dichotomous role of the serine/threonine kinase MAP4K4 in
            pancreatic ductal adenocarcinoma onset and metastasis through
            control of AKT and ERK pathways
  JOURNAL   J Pathol 262 (4), 454-466 (2024)
   PUBMED   38229581
  REMARK    GeneRIF: Dichotomous role of the serine/threonine kinase MAP4K4 in
            pancreatic ductal adenocarcinoma onset and metastasis through
            control of AKT and ERK pathways.
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   Huang,H., Kuang,X., Zou,Y., Zeng,J., Du,H., Tang,H., Long,C.,
            Mao,Y., Yu,X., Wen,C., Yan,J. and Shen,H.
  TITLE     MAP4K4 is involved in the neuronal development of retinal
            photoreceptors
  JOURNAL   Exp Eye Res 233, 109524 (2023)
   PUBMED   37290629
  REMARK    GeneRIF: MAP4K4 is involved in the neuronal development of retinal
            photoreceptors.
REFERENCE   3  (bases 1 to 5782)
  AUTHORS   Molla,M.R., Shimizu,A., Komeno,M., Rahman,N.I.A., Soh,J.E.C.,
            Nguyen,L.K.C., Khan,M.R., Tesega,W.W., Chen,S., Pang,X.,
            Tanaka-Okamoto,M., Takashima,N., Sato,A., Suzuki,T. and Ogita,H.
  TITLE     Vascular smooth muscle RhoA counteracts abdominal aortic aneurysm
            formation by modulating MAP4K4 activity
  JOURNAL   Commun Biol 5 (1), 1071 (2022)
   PUBMED   36207400
  REMARK    GeneRIF: Vascular smooth muscle RhoA counteracts abdominal aortic
            aneurysm formation by modulating MAP4K4 activity.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5782)
  AUTHORS   Lin,Y.C., Lu,Y.H., Lee,Y.C., Hung,C.S. and Lin,J.C.
  TITLE     Altered expressions and splicing profiles of Acin1 transcripts
            differentially modulate brown adipogenesis through an alternative
            splicing mechanism
  JOURNAL   Biochim Biophys Acta Gene Regul Mech 1863 (9), 194601 (2020)
   PUBMED   32629174
REFERENCE   5  (bases 1 to 5782)
  AUTHORS   Wu,C., Watts,M.E. and Rubin,L.L.
  TITLE     MAP4K4 Activation Mediates Motor Neuron Degeneration in Amyotrophic
            Lateral Sclerosis
  JOURNAL   Cell Rep 26 (5), 1143-1156 (2019)
   PUBMED   30699345
  REMARK    GeneRIF: These studies highlight a MAP4K4-initiated signaling
            cascade that induces motor neuron degeneration.
REFERENCE   6  (bases 1 to 5782)
  AUTHORS   Poinat,P., De Arcangelis,A., Sookhareea,S., Zhu,X., Hedgecock,E.M.,
            Labouesse,M. and Georges-Labouesse,E.
  TITLE     A conserved interaction between beta1 integrin/PAT-3 and
            Nck-interacting kinase/MIG-15 that mediates commissural axon
            navigation in C. elegans
  JOURNAL   Curr Biol 12 (8), 622-631 (2002)
   PUBMED   11967148
REFERENCE   7  (bases 1 to 5782)
  AUTHORS   Xue,Y., Wang,X., Li,Z., Gotoh,N., Chapman,D. and Skolnik,E.Y.
  TITLE     Mesodermal patterning defect in mice lacking the Ste20 NCK
            interacting kinase (NIK)
  JOURNAL   Development 128 (9), 1559-1572 (2001)
   PUBMED   11290295
REFERENCE   8  (bases 1 to 5782)
  AUTHORS   Becker,E., Huynh-Do,U., Holland,S., Pawson,T., Daniel,T.O. and
            Skolnik,E.Y.
  TITLE     Nck-interacting Ste20 kinase couples Eph receptors to c-Jun
            N-terminal kinase and integrin activation
  JOURNAL   Mol Cell Biol 20 (5), 1537-1545 (2000)
   PUBMED   10669731
REFERENCE   9  (bases 1 to 5782)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
REFERENCE   10 (bases 1 to 5782)
  AUTHORS   Su,Y.C., Han,J., Xu,S., Cobb,M. and Skolnik,E.Y.
  TITLE     NIK is a new Ste20-related kinase that binds NCK and MEKK1 and
            activates the SAPK/JNK cascade via a conserved regulatory domain
  JOURNAL   EMBO J 16 (6), 1279-1290 (1997)
   PUBMED   9135144
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ142598.1, BB651050.1, BC145390.1, U88984.1, AV445090.1,
            BQ571658.1, AK159799.1 and BF721981.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC145390.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-28                CJ142598.1         8-35
            29-266              BB651050.1         111-348             c
            267-1375            BC145390.1         1-1109
            1376-1856           U88984.1           1056-1536
            1857-3490           BC145390.1         1591-3224
            3491-3800           U88984.1           3009-3318
            3801-4226           BC145390.1         3535-3960
            4227-4602           AV445090.1         135-510
            4603-5106           BQ571658.1         60-563
            5107-5777           AK159799.1         366-1036
            5778-5782           BF721981.1         7-11                c
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 18.53 cM"
     gene            1..5782
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /note="mitogen-activated protein kinase kinase kinase
                     kinase 4"
                     /db_xref="GeneID:26921"
                     /db_xref="MGI:MGI:1349394"
     exon            1..374
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     CDS             318..4184
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1; MEKKK
                     4; MEK kinase kinase 4; nck-interacting kinase;
                     HPK/GCK-like kinase HGK; MAPK/ERK kinase kinase kinase 4;
                     NCK interacting kinase"
                     /codon_start=1
                     /product="mitogen-activated protein kinase kinase kinase
                     kinase 4 isoform 1"
                     /protein_id="NP_001239129.1"
                     /db_xref="CCDS:CCDS78573.1"
                     /db_xref="GeneID:26921"
                     /db_xref="MGI:MGI:1349394"
                     /translation="MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRH
                     VKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLW
                     LVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVL
                     LTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWS
                     CGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQ
                     RPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVP
                     EQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLL
                     AERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEE
                     RRRAEEEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQAMLLHDHRRPHAQQQPPP
                     PQQQDRSKPSFHAPEPKPHYDPADRAREVEDRFRKTNHSSPEAQAKQTGRGLEPPVPS
                     RSESFSNGNSESVHPALQRPAEPQVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHL
                     RSQDPCPPSRSEGLSQSSDSKSEVPEPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRR
                     DSPLQGGGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHP
                     GSQSGSGERFRVRSSSKSEGSPSPRQESAAKKPDDKKEVFRPLKPAGEVDLTALAKEL
                     RAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSPNLSNG
                     ETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLL
                     QISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKK
                     RFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVT
                     ISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVI
                     ALKSSVEVYAWAPKPYHKFMAFKSFGELLHKPLLVDLTVEEGQRLKVIYGSCAGFHAV
                     DVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVV
                     LQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKV
                     FFASVRSGGSSQVYFMTLGRTSLLSW"
     exon            375..440
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            441..497
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            498..623
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            624..734
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            735..825
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            826..956
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1011
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1090
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            1091..1266
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            1267..1339
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1550
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            1551..1712
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            1713..1799
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..1925
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            1926..2087
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            2088..2318
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            2319..2425
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            2426..2577
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            2578..2675
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            2676..2684
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            2685..2860
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            2861..2981
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            2982..3099
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            3100..3270
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            3271..3414
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            3415..3549
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            3550..3650
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            3651..3800
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            3801..3960
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            3961..4100
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     exon            4101..5782
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4399..4404
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /note="hexamer: AATAAA"
     polyA_site      4441
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /note="major polyA site"
     polyA_site      4968
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
     regulatory      5755..5760
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /note="hexamer: AATAAA"
     polyA_site      5778
                     /gene="Map4k4"
                     /gene_synonym="9430080K19Rik; HGK; Nik"
                     /note="major polyA site"
ORIGIN      
        1 gtctcgcggc ctcgcgctcg ctcgctcgcc gcgctctccg gccccagctc cgggccgctc
       61 gtgcaggcgc gcgcgcgcgg ggcgccgggg cctgaggcgg cgggcgacgc ccggggggcg
      121 ggtgggggcc gagggaggac cgccaagcca tggtgtgagc tccgcggcca gcgctctccg
      181 cgccgcgctc cgctccgacc ggacgacaag ccgcccgggc ggcggcggcc tgcccgtgcg
      241 gcccgagccc cggcgccgcc gagcccctgc ccgtctctcg ctctctcgcc cgctcccgga
      301 gcggcgaccc ggggaagatg gcgaacgact ctcccgcgaa gagcctggtg gacattgacc
      361 tgtcgtccct gcgggaccct gctgggattt ttgagctggt ggaagtggtt ggaaatggca
      421 cctatggaca agtctataag ggtcgacatg ttaaaacggg tcagctggcc gccatcaagg
      481 ttatggacgt caccgaggat gaagaggaag aaatcaaact ggagataaat atgctgaaga
      541 agtattctca tcatcgaaat attgccacgt actatggtgc tttcattaag aagagccctc
      601 caggacatga tgaccaactc tggcttgtta tggagttttg tggggctggg tccatcacag
      661 accttgtgaa gaacaccaaa gggaacactc tcaaagaaga ctggattgct tacatctcca
      721 gggaaatcct caggggattg gcacatctcc atattcacca cgttattcac cgagatatca
      781 agggccaaaa tgtgctgctg accgagaatg ctgaggtgaa acttgttgat tttggtgtaa
      841 gcgctcagct ggacaggacg gttggacgga gaaatacgtt cataggcaca ccctactgga
      901 tggctccaga ggtcatcgcc tgtgatgaga acccagacgc cacttacgac tacagaagtg
      961 acctctggtc ctgtggcatc acagccatcg agatggctga aggggccccc cctctctgtg
     1021 acatgcatcc aatgagagcg ctgtttctca tccccagaaa ccctcctccc aggctgaagt
     1081 caaaaaaatg gtcaaagaaa tttttcagct ttatagaagg ctgtctggtg aagaattaca
     1141 tgcagcggcc ctctacagag caacttttaa aacacccttt cataagggat cagcccaatg
     1201 aaaggcaggt tcgaatccag cttaaggatc acatagaccg gaccagaaag aagagaggcg
     1261 agaaagatga gacggagtac gagtacagcg ggagcgagga ggaggaggag gaagtgcctg
     1321 agcaggaggg agagccaagt tccatcgtca atgtgcctgg agagtcaact ctgcgacgtg
     1381 atttcctgag actgcagcag gagaacaagg agcggtctga ggctctgcgg agacagcagc
     1441 ttctgcagga gcagcagctc cgggagcagg aggagtataa gaggcagctg ctggctgaga
     1501 ggcagaagcg gattgaacag cagaaagaac agaggaggcg gctggaagag caacaaagaa
     1561 gagaacggga agccaggagg cagcaggagc gtgagcagcg gcggcgtgaa caagaggaga
     1621 agaggcgtct cgaggaactg gaaaggcggc gtaaagaaga ggaagagagg agacgggcag
     1681 aagaggagaa gaggagagtg gagagggaac aggagtacat caggcggcag ctagaggagg
     1741 agcagcggca cctggagatc ctgcagcagc agctgctcca ggagcaggcc atgttactgc
     1801 acgaccacag gaggccgcac gcacagcagc agccgccgcc cccgcagcag caggacagga
     1861 gcaaaccgag ctttcatgct ccagagccca agcctcacta tgaccctgct gacagagctc
     1921 gggaggtgga agatagattt agaaagacta accacagctc ccctgaggcc caggctaagc
     1981 aaacgggcag aggactggag ccacccgtgc cttcccggtc agagtctttt tccaatggca
     2041 actccgagtc tgtgcaccct gccctgcaga gaccagcgga gccacaggta cagtggtccc
     2101 acctggcatc tctcaagaac aatgtctccc ctgtctcgag atcccattcc ttcagtgacc
     2161 cttctcccaa attcgcacac caccatctcc gctctcagga cccatgtcca ccttcccgca
     2221 gtgaggggct cagtcagagc tctgactcta agtcggaggt gcccgagccc acccaaaagg
     2281 cttggtctag atcagacagt gacgaggtgc ctccaagggt tcccgtgaga acgacctctc
     2341 gttcccctgt cctctcccgt cgggattccc cactgcaggg cggtggacag cagaatagcc
     2401 aagcaggaca gagaaattcc accagcagta ttgagccccg gcttctttgg gagagagtgg
     2461 aaaagctggt ccccaggcca ggcagtggca gctcttcagg gtccagcaac tcaggatccc
     2521 agcctggctc ccatcctggg tctcagagcg gctccggtga acgcttcaga gtgagatcat
     2581 catccaagtc tgaaggttct ccatcgccac gccaggaaag tgcagccaaa aagcctgacg
     2641 ataagaaaga agtattccgg cctctcaagc ctgcgggaga agtggacttg actgcgttgg
     2701 ccaaagagct tcgagcagtg gaagatgttc ggccacctca caaagtaaca gactactcat
     2761 cctccagtga ggagtctggg accacagatg aggaggagga ggacgtggaa caggaggggg
     2821 ctgatgattc tacctcggga ccagaagaca ccagagcagc gtcatctccg aacctgagca
     2881 acggtgaaac agaatctgtg aaaacaatga tcgttcatga tgatgtagaa agtgagccag
     2941 ccatgacccc gtccaaggag ggcaccctca tcgtccgcca gactcagtcc gctagtagca
     3001 cactccagaa acacaaatct tcctcctcct ttacaccttt tatagacccc aggttactac
     3061 agatctctcc atctagtggg acgacagtga cttccgtggt gggattttcc tgtgatgggc
     3121 tgagaccaga agccataagg caagatccta ctcggaaagg ctcagtggtc aatgtgaacc
     3181 ccacgaacac taggccacag agtgataccc ccgagattcg taaatacaag aagagattta
     3241 actcagagat cctgtgtgct gccttatggg gagtgaactt gctggttggt acagagagcg
     3301 gtctgatgct cttggacaga agtggccaag ggaaagtata ccctctgatc agccgaagac
     3361 ggttccagca aatggatgtg ctcgaaggcc taaatgtcct ggtgacaata tctggcaaaa
     3421 aggataagtt acgtgtctac tatttatcct ggctaagaaa taagattctt cataatgatc
     3481 cagaggttga gaagaagcag gggtggacca ctgtgggcga cttggaaggg tgcgtgcact
     3541 ataaagtcgt aaaatatgaa agaatcaagt ttctggtaat tgctttgaag agttctgtgg
     3601 aagtctatgc atgggcaccg aagccatatc acaaatttat ggcctttaag tcatttggag
     3661 aactgctaca taagccatta ctggtggatc tcactgtgga ggaaggccag aggttgaaag
     3721 tgatctatgg atcctgtgcg ggattccatg ctgttgatgt ggattcaggc tcagtctatg
     3781 acatttatct accaacacat attcagtgta gcatcaaacc ccatgcaatc attatcctcc
     3841 ccaacacgga tgggatggag ctgctggtgt gctacgaaga cgagggggtt tacgttaaca
     3901 cgtacggaag gatcaccaag gacgtggttc tgcagtgggg agagatgccg acatctgtag
     3961 catatatccg gtcgaatcag acaatgggct ggggagagaa ggcaatagag atacgatctg
     4021 tggaaactgg tcacttggat ggtgtattta tgcacaaaag ggctcagaga ctaaagttcc
     4081 tgtgtgaacg caatgacaag gtgttctttg cctccgtccg ctctggtggc agcagccagg
     4141 tctatttcat gaccttaggc aggacttctc ttctgagctg gtagaggcag tgtgagctgg
     4201 gatcactgac ctctggagtc ggcaagaccc tgagcgagac cttggaattc cttgcaactg
     4261 gagctcagag ctgtgctggt gcagccagga cagctgtctg caggccacct gcgcgtcgca
     4321 tcgggtcggg tcgggccctc tcccctccct cctacacttt ttgccagttt attcccattc
     4381 tctccttttc ctttctcaaa taaatcaagg ctgcattgca gctggtgctg tctggactcc
     4441 accatcggtg ctaggagtgc ttgggaatgg actcagaccg ggaagagcga gtgagcgagc
     4501 gagcgctgcg ccttccctgc agctccagga ggccgtctcc gagcgagcga gcgagcgagc
     4561 gactgtccta tgggtgtctg aagggtgtga tgagcgcacc gtcagacttt ggcagtgggc
     4621 acaaagaagt gaaaagtggg cgtaagaagc agcttgcaga agcagagaac agatttaata
     4681 tagtaacatt aacaatgtat ttaattgaca tttctctttt tgtaatgtga cagtatgtgg
     4741 acagagaaga aggtgcaggt ttaagaagtt aatatttata aaatgtgaaa gacacagtta
     4801 ctaggatacc ttttttttgt gggtggggct tagggtgggg tggggtgggt taaagggtcc
     4861 cattttgttt ctttgaaagt tttttggagg ggattgtcct ggccaagaac tcagtcattt
     4921 ttctgtgtac caagttttgc ctaaattgtg caggtggttc ttaaaaaaga aagaaaagga
     4981 aaagaaaagt gtgtgtcttg ttggagggcc agaagtttgt cgtgtgacag tattagcccg
     5041 gcctcggcta caggtttaaa gtctgttagg cctaagtgca gctccccgtg gcctgcacac
     5101 accgtaggag aaggtcagct ttcctgtggg gttttgtttc attttgtttt gttttttgcc
     5161 tacctcactg tttttaatgc attgagaggt gacttagttt atggttttgg cagaaaacat
     5221 taatgtttaa tttaacctta ataccaaaac tatctgatta aagtttgact ttgtcacaag
     5281 tctaaatcag gtacaaaaaa ggaaaaaaac aaaaaataaa aaacaagcct gtctatgaca
     5341 gcagggagta gccttgtagc tgctgcagtc tgcttctagc atgcagactg ctctgttctc
     5401 accaagttca ccattagcgg gtgacaggca gccagctgcc acctgtgaca cataagagat
     5461 gtcattgttc tcagttgggt ttggaagcag gcaggggtca cactggggag tcacacagac
     5521 tgttgatgtt gcaggagcct ttcttgggag tgacggtgtg tcccttttct aagaatgcaa
     5581 tgcactaaaa ctgttttaag aatgtagttt gccctacttc ttcatagatg gcccctccct
     5641 gcctgtgttt caggtgtaaa atcgacactc tggcttttct ccttttctca cgtgctctct
     5701 tgttctcttt ttttaaaact aatttttctt tatgaatatg tctgtgacat ttgtaataaa
     5761 tgtcttgtaa taatttgctt ca
//
